(Circular) (Six-base) MAPSORT of: pFN21AE5254.seq Check: 5769 from: 1 to: 5341 pFN21AE5254 5341 bp With 182 enzymes: SgfI * January 30, 2014 17:16 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3151 278 Size: 53 83 186 936 1615 2468 Fragments arranged by size: 2468 1615 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2684 1937 Size: 747 4594 Acc65I G'GTAC_C Cuts at: 2662 2662 Size: 5341 AclI AA'CG_TT Cuts at: 1563 3468 3841 1563 Size: 1905 373 3063 Fragments arranged by size: 3063 1905 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3404 4788 5056 1632 Size: 1772 1384 268 1917 Fragments arranged by size: 1917 1772 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2093 820 Size: 197 1076 4068 Fragments arranged by size: 4068 1076 197 AflIII A'CryG_T Cuts at: 1203 2385 2401 4240 5217 1203 Size: 1182 16 1839 977 1327 Fragments arranged by size: 1839 1327 1182 977 16 AgeI A'CCGG_T Cuts at: 4172 4172 Size: 5341 AhdI GACnn_n'nnGTC Cuts at: 4070 4070 Size: 5341 AleI CACnn'nnGTG Cuts at: 1166 2486 1166 Size: 1320 4021 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5341 AlwNI CAG_nnn'CTG Cuts at: 3084 4656 3084 Size: 1572 3769 ApaI G_GGCC'C Cuts at: 2140 2140 Size: 5341 ApaLI G'TGCA_C Cuts at: 3398 4554 3398 Size: 1156 4185 ApoI r'AATT_y Cuts at: 1493 2650 2876 3028 5137 1493 Size: 1157 226 152 2109 1697 Fragments arranged by size: 2109 1697 1157 226 152 AseI AT'TA_AT Cuts at: 160 1032 3895 160 Size: 872 2863 1606 Fragments arranged by size: 2863 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5341 AvaI C'yCGr_G Cuts at: 1870 1943 2333 2666 1870 Size: 73 390 333 4545 Fragments arranged by size: 4545 390 333 73 BamHI G'GATC_C Cuts at: 2671 3038 2671 Size: 367 4974 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2662 4117 618 Size: 325 217 627 875 1455 1842 Fragments arranged by size: 1842 1455 875 627 325 217 BanII G_rGCy'C Cuts at: 1391 2032 2140 2660 1391 Size: 641 108 520 4072 Fragments arranged by size: 4072 641 520 108 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4370 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3532 3566 1903 Size: 34 1595 34 3678 Fragments arranged by size: 3678 1595 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2351 3233 4443 2351 Size: 882 1210 3249 Fragments arranged by size: 3249 1210 882 BclI T'GATC_A Cuts at: 1548 1548 Size: 5341 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3952 136 Size: 107 122 71 631 605 2280 1525 Fragments arranged by size: 2280 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5336 5336 Size: 5341 BlpI GC'TnA_GC Cuts at: 2758 2758 Size: 5341 Bme1580I G_kGCm'C Cuts at: 1790 2140 2275 3402 4558 1790 Size: 350 135 1127 1156 2573 Fragments arranged by size: 2573 1156 1127 350 135 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5341 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3072 4030 442 Size: 416 53 83 399 24 1655 958 1753 Fragments arranged by size: 1753 1655 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5341 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2168 4001 1239 Size: 929 1833 2579 Fragments arranged by size: 2579 1833 929 Bpu10I CC'TnA_GC Cuts at: 2024 2417 2024 Size: 393 4948 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3466 4331 4629 3466 Size: 865 298 4178 Fragments arranged by size: 4178 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2270 4004 882 Size: 617 771 1734 2219 Fragments arranged by size: 2219 1734 771 617 BsaAI yAC'GTr Cuts at: 493 1204 3132 493 Size: 711 1928 2702 Fragments arranged by size: 2702 1928 711 BsaBI GATnn'nnATC Cuts at: 5335 5335 Size: 5341 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3148 3530 4165 275 Size: 53 83 186 936 1615 382 635 1451 Fragments arranged by size: 1615 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2103 2169 3774 4172 4446 4593 5004 2103 Size: 66 1605 398 274 147 411 2440 Fragments arranged by size: 2440 1605 411 398 274 147 66 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2180 2210 1178 Size: 30 165 30 777 30 4309 Fragments arranged by size: 4309 777 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5341 BseYI C'CCAG_C Cuts at: 2305 4544 5040 2305 Size: 2239 496 2606 Fragments arranged by size: 2606 2239 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4768 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2791 3552 4580 1540 Size: 275 186 790 761 1028 2301 Fragments arranged by size: 2301 1028 790 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3402 3487 4558 3402 Size: 85 1071 4185 Fragments arranged by size: 4185 1071 85 BsmI GAATG_Cn' Cuts at: 2315 2852 2945 2315 Size: 537 93 4711 Fragments arranged by size: 4711 537 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2032 2140 2275 2660 3402 3487 4558 Size: 399 242 108 135 385 742 85 1071 Cuts at: 4558 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 385 242 135 108 85 BspEI T'CCGG_A Cuts at: 2103 2169 5004 2103 Size: 66 2835 2440 Fragments arranged by size: 2835 2440 66 BspHI T'CATG_A Cuts at: 2154 3230 4960 2154 Size: 1076 1730 2535 Fragments arranged by size: 2535 1730 1076 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2696 844 Size: 1852 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3228 1394 Size: 429 1405 3507 Fragments arranged by size: 3507 1405 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2590 3836 4010 65 Size: 1183 1342 1246 174 1396 Fragments arranged by size: 1396 1342 1246 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3985 4172 1700 Size: 110 2175 187 2869 Fragments arranged by size: 2869 2175 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5341 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5341 BssSI C'ACGA_G Cuts at: 3401 4413 3401 Size: 1012 4329 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5341 BstBI TT'CG_AA Cuts at: 4145 4145 Size: 5341 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5341 BstYI r'GATC_y Cuts at: 1972 2671 3038 3423 3440 4892 4978 4990 5336 Size: 699 367 385 17 1452 86 12 346 Cuts at: 5336 1972 Size: 1977 Fragments arranged by size: 1977 1452 699 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3850 Fragments arranged by size: 3850 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4602 Fragments arranged by size: 4602 683 56 BtsI GCAGTG_nn' Cuts at: 2051 2481 2857 3651 3671 2051 Size: 430 376 794 20 3721 Fragments arranged by size: 3721 794 430 376 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5341 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5306 DraI TTT'AAA Cuts at: 2645 2645 Size: 5341 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4348 809 Size: 507 3032 1802 Fragments arranged by size: 3032 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2085 2788 3677 Size: 54 1425 50 275 18 255 703 889 Cuts at: 3677 5306 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 703 275 255 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2788 1537 Size: 275 976 4090 Fragments arranged by size: 4090 976 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2171 3271 1353 Size: 818 1100 3423 Fragments arranged by size: 3423 1100 818 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3928 4302 4448 387 Size: 1064 138 2339 374 146 1280 Fragments arranged by size: 2339 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2168 3404 4001 4788 5056 1239 Size: 393 536 1236 597 787 268 1524 Fragments arranged by size: 1524 1236 787 597 536 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2573 2573 Size: 5341 EcoO109I rG'GnC_Cy Cuts at: 2137 2464 2137 Size: 327 5014 EcoRI G'AATT_C Cuts at: 2650 3028 2650 Size: 378 4963 FspI TGC'GCA Cuts at: 2060 3847 2060 Size: 1787 3554 HaeII r_GCGC'y Cuts at: 1678 2068 4488 1678 Size: 390 2420 2531 Fragments arranged by size: 2531 2420 390 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2115 2147 3982 4014 4056 Size: 32 135 32 543 32 1835 32 42 Cuts at: 4056 4088 1373 Size: 32 2626 Fragments arranged by size: 2626 1835 543 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2047 2685 2931 669 Size: 988 281 109 638 246 3079 Fragments arranged by size: 3079 988 638 281 246 109 HindIII A'AGCT_T Cuts at: 748 748 Size: 5341 HpaI GTT'AAC Cuts at: 2931 2931 Size: 5341 KpnI G_GTAC'C Cuts at: 2666 2666 Size: 5341 MfeI C'AATT_G Cuts at: 2940 2940 Size: 5341 MluI A'CGCG_T Cuts at: 2401 5217 2401 Size: 2816 2525 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4455 4639 1305 Size: 3150 184 2007 Fragments arranged by size: 3150 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3519 Fragments arranged by size: 3519 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2486 3299 3658 3817 518 Size: 648 1320 813 359 159 2042 Fragments arranged by size: 2042 1320 813 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2476 2757 3434 4582 4827 1606 Size: 870 281 677 1148 245 2120 Fragments arranged by size: 2120 1148 870 677 281 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3850 Fragments arranged by size: 3850 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5341 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5341 NotI GC'GGCC_GC Cuts at: 2788 2788 Size: 5341 NspI r_CATG'y Cuts at: 1132 1229 2389 2699 4244 1132 Size: 97 1160 310 1545 2229 Fragments arranged by size: 2229 1545 1160 310 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5341 PciI A'CATG_T Cuts at: 2385 4240 2385 Size: 1855 3486 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5341 PfoI T'CCnGG_A Cuts at: 2184 2228 2184 Size: 44 5297 PmeI GTTT'AAAC Cuts at: 2645 2645 Size: 5341 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3410 3442 4923 4955 3410 Size: 32 1481 32 3796 Fragments arranged by size: 3796 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2464 2464 Size: 5341 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5341 PsiI TTA'TAA Cuts at: 2911 2911 Size: 5341 PspOMI G'GGCC_C Cuts at: 2136 2136 Size: 5341 PstI C_TGCA'G Cuts at: 830 2211 2280 2541 2553 2693 830 Size: 1381 69 261 12 140 3478 Fragments arranged by size: 3478 1381 261 140 69 12 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5341 SalI G'TCGA_C Cuts at: 1936 2683 1936 Size: 747 4594 SanDI GG'GwC_CC Cuts at: 2464 2464 Size: 5341 SbfI CC_TGCA'GG Cuts at: 2693 2693 Size: 5341 ScaI AGT'ACT Cuts at: 3589 5301 3589 Size: 1712 3629 SfcI C'TryA_G Cuts at: 826 1045 2207 2276 2430 2537 2549 2689 3824 Size: 219 1162 69 154 107 12 140 1135 Cuts at: 3824 4505 4696 826 Size: 681 191 1471 Fragments arranged by size: 1471 1162 1135 681 219 191 154 140 107 69 12 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5341 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5341 SmaI CCC'GGG Cuts at: 1872 2668 1872 Size: 796 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2093 3445 4346 4608 820 Size: 197 926 150 1352 901 262 1553 Fragments arranged by size: 1553 1352 926 901 262 197 150 SnaBI TAC'GTA Cuts at: 493 3132 493 Size: 2639 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5341 SphI G_CATG'C Cuts at: 1132 2699 1132 Size: 1567 3774 SspI AAT'ATT Cuts at: 5 52 3265 5 Size: 47 3213 2081 Fragments arranged by size: 3213 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5341 StyI C'CwwG_G Cuts at: 513 1065 2004 2780 513 Size: 552 939 776 3074 Fragments arranged by size: 3074 939 776 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3368 3385 3538 3723 4168 325 Size: 1255 1788 17 153 185 445 1498 Fragments arranged by size: 1788 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3587 5299 96 Size: 274 80 33 51 1443 1610 1712 138 Fragments arranged by size: 1712 1610 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2180 2212 2301 2333 5301 5333 2180 Size: 32 89 32 2968 32 2188 Fragments arranged by size: 2968 2188 89 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5341 XbaI T'CTAG_A Cuts at: 2677 2677 Size: 5341 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5341 XmaI C'CCGG_G Cuts at: 1870 2666 1870 Size: 796 4545 XmnI GAAnn'nnTTC Cuts at: 3470 3470 Size: 5341 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3149 276 Size: 53 83 186 936 1615 2468 Fragments arranged by size: 2468 1615 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI