Restriction map for
pFN21AE5239
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# NO CUT ENZYMES ( 152 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AviII AvrII BanI BbeI
BbrPI BcgI BciVI BclI BcnI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HapII HgaI HhaI HinP1I HpaII
KasI KpnI Ksp632I KspI MaeI
MamI MfeI MluI MroI MspI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfaNI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI Tth111I TthHB8I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 285 GACGTC GACGT^C
AciI 214 CCGC CCGC(-3/-1)
AcsI 563 AAATTT [GA]^AATT[CT]
AcyI 285 GACGTC G[GA]^CG[CT]C
AfaI 245 GTAC GT^AC
AflIII 665 ACGTGT A^C[GA][CT]GT
ApoI 563 AAATTT [GA]^AATT[CT]
Asp700I 205 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 375 TGGCCA TGG^CCA
BamHI 515 GGATCC G^GATCC
BanII 366 GAGCCC G[GA]GC[CT]^C
BbvCI 162 CCTCAGC CCTCAGC(-5/-2)
BbvI 342 GCTGC GCAGC(8/12)
BpmI 236 CTGGAG CTGGAG(16/14)
BsaHI 285 GACGTC G[GA]^CG[CT]C
BseRI 239 GAGGAG GAGGAG(10/8)
BsmAI 383 GTCTC GTCTC(1/5)
BsmBI 382 CGTCTC CGTCTC(1/5)
BsmI 197 GCATTC GAATGC(1/-1)
BsrBI 214 CCGCTC CCGCTC(-3/-3)
BsrDI 167 GCAATG GCAATG(2/0)
BstAPI 662 GCAACGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 543 CCATTCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 319 GACATAAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 375 TGGCCA [CT]^GGCC[GA]
EcoNI 235 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Fnu4HI 342 GCTGC GC^[ACGT]GC
Hin1I 285 GACGTC G[GA]^CG[CT]C
HincII 727 GTTAAC GT[CT]^[GA]AC
HindII 727 GTTAAC GT[CT]^[GA]AC
HindIII 147 AAGCTT A^AGCTT
HpaI 727 GTTAAC GTT^AAC
ItaI 342 GCTGC GC^[ACGT]GC
MluNI 375 TGGCCA TGG^CCA
MscI 375 TGGCCA TGG^CCA
MseI 728 TTAA T^TAA
MslI 708 CACCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 340 CAGCTG C[AC]G^C[GT]G
NcoI 50 CCATGG C^CATGG
NspI 731 ACATGC [GA]CATG^[CT]
PleI 126 GAGTC GAGTC(4/5)
PstI 479 CTGCAG CTGCA^G
PvuII 340 CAGCTG CAG^CTG
RsaI 245 GTAC GT^AC
SexAI 521 ACCTGGT A^CC[AT]GGT
SmlI 123 CTTGAG C^T[CT][GA]AG
StuI 66 AGGCCT AGG^CCT
Tru9I 728 TTAA T^TAA
TseI 342 GCTGC G^C[AT]GC
Tsp509I 564 AATT ^AATT
XcmI 519 CCACCTGGTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 205 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
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AlwNI 88 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
340 CAGCTGCTG
AvaII 84 GGACC G^G[AT]CC
525 GGTCC
BbsI 25 GTCTTC GAAGAC(2/6)
605 GTCTTC
BglI 188 GCCTGTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
368 GCCCTGATGGC
BpuAI 25 GTCTTC GAAGAC(2/6)
605 GTCTTC
BsgI 537 CTGCAC GTGCAG(16/14)
361 GTGCAG
BsmFI 267 GGGAC GGGAC(10/14)
128 GTCCC
BsrI 10 ACTGG ACTGG(1/-1)
57 CCAGT
BstF5I 225 CATCC GGATG(2/0)
396 CATCC
BstYI 515 GGATCC [GA]^GATC[CT]
626 GGATCT
DraII 14 GGGGCCT [GA]G^G[ACGT]CC[CT]
185 GGGGCCT
DsaI 50 CCATGG C^C[GA][CT]GG
741 CCACGG
EcoO109I 14 GGGGCCT [GA]G^G[ACGT]CC[CT]
185 GGGGCCT
FokI 225 CATCC GGATG(9/13)
396 CATCC
MaeII 286 ACGT A^CGT
665 ACGT
MboII 26 TCTTC GAAGA(8/7)
606 TCTTC
MflI 515 GGATCC [GA]^GATC[CT]
626 GGATCT
PflMI 45 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
339 CCAGCTGCTGG
SfcI 479 CTGCAG C^T[GA][CT]AG
748 CTACAG
TfiI 108 GAATC G^A[AT]TC
294 GATTC
Van91I 45 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
339 CCAGCTGCTGG
XhoII 515 GGATCC [GA]^GATC[CT]
626 GGATCT
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# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AluI AlwI BmyI BsaJI BsiYI
BslI Bsp1286I BssKI BstNI Cac8I
Cfr13I DdeI DpnII EcoRII EcoT14I
HaeIII HinfI HphI MaeIII MboI
MnlI MvaI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI StyI
Tsp45I TspRI