(Circular) (Six-base) MAPSORT of: pFN21AE5239.seq Check: 5207 from: 1 to: 5473 pFN21AE5239 5473 bp With 182 enzymes: SgfI * January 30, 2014 17:15 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2294 3283 278 Size: 53 83 186 936 758 989 2468 Fragments arranged by size: 2468 989 936 758 186 83 53 AccI GT'mk_AC Cuts at: 1937 2816 1937 Size: 879 4594 Acc65I G'GTAC_C Cuts at: 2794 2794 Size: 5473 AclI AA'CG_TT Cuts at: 1563 3600 3973 1563 Size: 2037 373 3063 Fragments arranged by size: 3063 2037 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2255 3536 4920 5188 1632 Size: 623 1281 1384 268 1917 Fragments arranged by size: 1917 1384 1281 623 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5276 AflIII A'CryG_T Cuts at: 1203 2670 4372 5349 1203 Size: 1467 1702 977 1327 Fragments arranged by size: 1702 1467 1327 977 AgeI A'CCGG_T Cuts at: 4304 4304 Size: 5473 AhdI GACnn_n'nnGTC Cuts at: 4202 4202 Size: 5473 AleI CACnn'nnGTG Cuts at: 1166 2717 1166 Size: 1551 3922 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2195 1184 Size: 1011 4462 AlwNI CAG_nnn'CTG Cuts at: 2098 2350 3216 4788 2098 Size: 252 866 1572 2783 Fragments arranged by size: 2783 1572 866 252 ApaLI G'TGCA_C Cuts at: 3530 4686 3530 Size: 1156 4317 ApoI r'AATT_y Cuts at: 1493 2568 2782 3008 3160 5269 1493 Size: 1075 214 226 152 2109 1697 Fragments arranged by size: 2109 1697 1075 226 214 152 AseI AT'TA_AT Cuts at: 160 1032 4027 160 Size: 872 2995 1606 Fragments arranged by size: 2995 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5473 AvaI C'yCGr_G Cuts at: 1870 1943 2798 1870 Size: 73 855 4545 Fragments arranged by size: 4545 855 73 BamHI G'GATC_C Cuts at: 2520 2803 3170 2520 Size: 283 367 4823 Fragments arranged by size: 4823 367 283 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2794 4249 618 Size: 325 217 627 1007 1455 1842 Fragments arranged by size: 1842 1455 1007 627 325 217 BanII G_rGCy'C Cuts at: 1391 2375 2792 1391 Size: 984 417 4072 Fragments arranged by size: 4072 984 417 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2023 2603 928 Size: 971 124 580 3798 Fragments arranged by size: 3798 971 580 124 BbvCI CC'TCA_GC Cuts at: 2168 2168 Size: 5473 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3664 3698 1903 Size: 34 1727 34 3678 Fragments arranged by size: 3678 1727 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3365 4575 3365 Size: 1210 4263 BclI T'GATC_A Cuts at: 1548 1548 Size: 5473 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2199 2379 4084 Size: 107 122 71 631 605 527 180 1705 Cuts at: 4084 136 Size: 1525 Fragments arranged by size: 1705 1525 631 605 527 180 122 107 71 BglII A'GATC_T Cuts at: 5468 5468 Size: 5473 BlpI GC'TnA_GC Cuts at: 2890 2890 Size: 5473 Bme1580I G_kGCm'C Cuts at: 1790 2085 2678 3534 4690 1790 Size: 295 593 856 1156 2573 Fragments arranged by size: 2573 1156 856 593 295 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5473 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2025 2056 3204 Size: 416 53 83 399 24 608 31 1148 Cuts at: 3204 4162 442 Size: 958 1753 Fragments arranged by size: 1753 1148 958 608 416 399 83 53 31 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5473 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2262 4133 1239 Size: 1023 1871 2579 Fragments arranged by size: 2579 1871 1023 Bpu10I CC'TnA_GC Cuts at: 2168 2168 Size: 5473 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2149 3598 4463 4761 2149 Size: 1449 865 298 2861 Fragments arranged by size: 2861 1449 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4136 882 Size: 617 2637 2219 Fragments arranged by size: 2637 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3264 493 Size: 711 2060 2702 Fragments arranged by size: 2702 2060 711 BsaBI GATnn'nnATC Cuts at: 5467 5467 Size: 5473 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2291 3280 3662 4297 Size: 53 83 186 936 758 989 382 635 Cuts at: 4297 275 Size: 1451 Fragments arranged by size: 1451 989 936 758 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3906 4304 4578 4725 5136 3906 Size: 398 274 147 411 4243 Fragments arranged by size: 4243 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5248 Fragments arranged by size: 5248 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2259 1186 Size: 1073 4400 BseYI C'CCAG_C Cuts at: 2343 4676 5172 2343 Size: 2333 496 2644 Fragments arranged by size: 2644 2333 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2387 2527 1163 Size: 573 651 140 4109 Fragments arranged by size: 4109 651 573 140 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2923 3684 4712 1540 Size: 275 186 922 761 1028 2301 Fragments arranged by size: 2301 1028 922 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3534 3619 4690 3534 Size: 85 1071 4317 Fragments arranged by size: 4317 1071 85 BsmI GAATG_Cn' Cuts at: 2202 2984 3077 2202 Size: 782 93 4598 Fragments arranged by size: 4598 782 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2393 2393 Size: 5473 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2085 2375 2678 2792 3534 3619 4690 Size: 399 295 290 303 114 742 85 1071 Cuts at: 4690 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 303 295 290 114 85 BspEI T'CCGG_A Cuts at: 5136 5136 Size: 5473 BspHI T'CATG_A Cuts at: 3362 5092 3362 Size: 1730 3743 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2828 844 Size: 1984 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2221 3360 1394 Size: 429 398 1139 3507 Fragments arranged by size: 3507 1139 429 398 BsrDI GCAATG_nn' Cuts at: 65 1248 2179 3968 4142 65 Size: 1183 931 1789 174 1396 Fragments arranged by size: 1789 1396 1183 931 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4117 4304 1700 Size: 110 2307 187 2869 Fragments arranged by size: 2869 2307 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5473 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5473 BssSI C'ACGA_G Cuts at: 3533 4545 3533 Size: 1012 4461 BstAPI GCAn_nnn'nTGC Cuts at: 794 2673 794 Size: 1879 3594 BstBI TT'CG_AA Cuts at: 4277 4277 Size: 5473 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5473 BstXI CCAn_nnnn'nTGG Cuts at: 2555 2555 Size: 5473 BstYI r'GATC_y Cuts at: 1972 2520 2631 2803 3170 3555 3572 5024 5110 Size: 548 111 172 367 385 17 1452 86 Cuts at: 5110 5122 5468 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 548 385 367 346 172 111 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2055 2746 513 Size: 552 990 691 3240 Fragments arranged by size: 3240 990 691 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4734 Fragments arranged by size: 4734 683 56 BtsI GCAGTG_nn' Cuts at: 2481 2989 3783 3803 2481 Size: 508 794 20 4151 Fragments arranged by size: 4151 794 508 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5473 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2125 2160 529 Size: 35 1561 35 3842 Fragments arranged by size: 3842 1561 35 35 DraI TTT'AAA Cuts at: 2777 2777 Size: 5473 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2330 4480 809 Size: 507 1014 2150 1802 Fragments arranged by size: 2150 1802 1014 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2380 2920 3809 Size: 54 1425 50 275 18 550 540 889 Cuts at: 3809 5438 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 550 540 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2920 1537 Size: 275 1108 4090 Fragments arranged by size: 4090 1108 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3403 1353 Size: 2050 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4060 4434 4580 387 Size: 1064 138 2471 374 146 1280 Fragments arranged by size: 2471 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2255 2262 3536 4133 4920 5188 1239 Size: 393 623 7 1274 597 787 268 1524 Fragments arranged by size: 1524 1274 787 623 597 393 268 7 EcoNI CCTnn'n_nnAGG Cuts at: 2244 2244 Size: 5473 EcoO109I rG'GnC_Cy Cuts at: 2020 2191 2020 Size: 171 5302 EcoRI G'AATT_C Cuts at: 2782 3160 2782 Size: 378 5095 FspI TGC'GCA Cuts at: 3979 3979 Size: 5473 HaeII r_GCGC'y Cuts at: 1678 4620 1678 Size: 2942 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4114 4146 4188 4220 1373 Size: 32 135 32 2542 32 42 32 2626 Fragments arranged by size: 2626 2542 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2734 2817 3063 669 Size: 988 281 796 83 246 3079 Fragments arranged by size: 3079 988 796 281 246 83 HindIII A'AGCT_T Cuts at: 748 2152 748 Size: 1404 4069 HpaI GTT'AAC Cuts at: 2734 3063 2734 Size: 329 5144 KpnI G_GTAC'C Cuts at: 2798 2798 Size: 5473 MfeI C'AATT_G Cuts at: 3072 3072 Size: 5473 MluI A'CGCG_T Cuts at: 5349 5349 Size: 5473 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4587 4771 1305 Size: 3282 184 2007 Fragments arranged by size: 3282 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2382 10 Size: 54 1425 343 550 3101 Fragments arranged by size: 3101 1425 550 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2717 3431 3790 3949 518 Size: 648 1551 714 359 159 2042 Fragments arranged by size: 2042 1551 714 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2347 2889 3566 4714 4959 1606 Size: 741 542 677 1148 245 2120 Fragments arranged by size: 2120 1148 741 677 542 245 NcoI C'CATG_G Cuts at: 513 1065 2055 513 Size: 552 990 3931 Fragments arranged by size: 3931 990 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5473 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5473 NotI GC'GGCC_GC Cuts at: 2920 2920 Size: 5473 NspI r_CATG'y Cuts at: 1132 1229 2740 2831 4376 1132 Size: 97 1511 91 1545 2229 Fragments arranged by size: 2229 1545 1511 97 91 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5473 PciI A'CATG_T Cuts at: 4372 4372 Size: 5473 PflMI CCAn_nnn'nTGG Cuts at: 1108 2056 2350 1108 Size: 948 294 4231 Fragments arranged by size: 4231 948 294 PfoI T'CCnGG_A Cuts at: 2223 2223 Size: 5473 PmeI GTTT'AAAC Cuts at: 2777 2777 Size: 5473 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3542 3574 5055 5087 3542 Size: 32 1481 32 3928 Fragments arranged by size: 3928 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5473 PsiI TTA'TAA Cuts at: 3043 3043 Size: 5473 PstI C_TGCA'G Cuts at: 830 2488 2825 830 Size: 1658 337 3478 Fragments arranged by size: 3478 1658 337 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5473 PvuII CAG'CTG Cuts at: 2347 2347 Size: 5473 SalI G'TCGA_C Cuts at: 1936 2815 1936 Size: 879 4594 SbfI CC_TGCA'GG Cuts at: 2825 2825 Size: 5473 ScaI AGT'ACT Cuts at: 3721 5433 3721 Size: 1712 3761 SexAI A'CCwGG_T Cuts at: 2526 2526 Size: 5473 SfcI C'TryA_G Cuts at: 826 1045 2484 2753 2821 3956 4637 4828 826 Size: 219 1439 269 68 1135 681 191 1471 Fragments arranged by size: 1471 1439 1135 681 269 219 191 68 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5473 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5473 SmaI CCC'GGG Cuts at: 1872 2800 1872 Size: 928 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2128 3577 4478 4740 820 Size: 197 926 185 1449 901 262 1553 Fragments arranged by size: 1553 1449 926 901 262 197 185 SnaBI TAC'GTA Cuts at: 493 3264 493 Size: 2771 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5473 SphI G_CATG'C Cuts at: 1132 2831 1132 Size: 1699 3774 SspI AAT'ATT Cuts at: 5 52 3397 5 Size: 47 3345 2081 Fragments arranged by size: 3345 2081 47 StuI AGG'CCT Cuts at: 1517 2073 1517 Size: 556 4917 StyI C'CwwG_G Cuts at: 513 1065 2055 2266 2533 2912 513 Size: 552 990 211 267 379 3074 Fragments arranged by size: 3074 990 552 379 267 211 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2561 3500 3517 3670 3855 4300 325 Size: 1255 981 939 17 153 185 445 1498 Fragments arranged by size: 1498 1255 981 939 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3719 5431 96 Size: 274 80 33 51 1443 1742 1712 138 Fragments arranged by size: 1742 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5433 5465 5433 Size: 32 5441 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5473 XbaI T'CTAG_A Cuts at: 2809 2809 Size: 5473 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2531 2531 Size: 5473 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5473 XmaI C'CCGG_G Cuts at: 1870 2798 1870 Size: 928 4545 XmnI GAAnn'nnTTC Cuts at: 2214 3602 2214 Size: 1388 4085 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2292 3281 276 Size: 53 83 186 936 758 989 2468 Fragments arranged by size: 2468 989 936 758 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI