################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI ApaI ApoI AscI AseI AsnI Asp700I AspEI AspI AvaII AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstYI BstZ17I CelII ClaI CpoI DraI DraII DraIII DrdI DsaI Eam1105I EcoNI EcoO109I EcoO65I EcoRI EcoT22I FbaI FseI FspI Hin1I HincII HindII HpaI KasI KspI MamI MfeI MflI MluI MluNI MroI MscI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### Acc65I 180 GGTACC G^GTACC Alw44I 256 GTGCAC G^TGCAC AlwI 69 GGATC GGATC(4/5) AocI 175 CCTGAGG CC^T[ACGT]AGG ApaLI 256 GTGCAC G^TGCAC Asp718I 180 GGTACC G^GTACC AspHI 256 GTGCAC G[AT]GC[AT]^C AvaI 614 CCCGGG C^[CT]CG[GA]G BcgI 155 GCATCCCGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 325 CTAG C^TAG BpmI 448 CTGGAG CTGGAG(16/14) BsiHKAI 256 GTGCAC G[AT]GC[AT]^C BsoBI 614 CCCGGG C^[CT]CG[GA]G BsrDI 5 GCAATG GCAATG(2/0) BsrI 422 CCAGT ACTGG(1/-1) BstAPI 515 GCAAGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 59 CCAAAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 175 CCTGAGG CC^T[ACGT]AGG EaeI 628 CGGCCG [CT]^GGCC[GA] EagI 628 CGGCCG C^GGCCG EarI 406 GAAGAG CTCTTC(1/4) EclXI 628 CGGCCG C^GGCCG Eco52I 628 CGGCCG C^GGCCG Eco81I 175 CCTGAGG CC^T[ACGT]AGG EcoRV 139 GATATC GAT^ATC EcoT14I 17 CCAAGG C^C[AT][AT]GG HgaI 650 GCGTC GACGC(5/10) HindIII 433 AAGCTT A^AGCTT KpnI 180 GGTACC GGTAC^C Ksp632I 406 GAAGAG CTCTTC(1/4) MaeI 325 CTAG C^TAG MaeII 338 ACGT A^CGT MboII 406 GAAGA GAAGA(8/7) MseI 236 TTAA T^TAA MslI 288 CACGATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG SmaI 614 CCCGGG CCC^GGG StyI 17 CCAAGG C^C[AT][AT]GG Tru9I 236 TTAA T^TAA XmaCI 614 CCCGGG C^CCGGG XmaI 614 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AfaI 22 GTAC GT^AC 181 GTAC AlwNI 124 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG 592 CAGCGCCTG Aor51HI 455 AGCGCT AGC^GCT 518 AGCGCT BanI 180 GGTACC G^G[CT][GA]CC 600 GGTGCC BanII 468 GAGCCC G[GA]GC[CT]^C 531 GGGCTC BglI 349 GCCATCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 524 GCCGGCAGGGC BsiEI 161 CGGTCG CG[GA][CT]^CG 628 CGGCCG BsmFI 335 GGGAC GGGAC(10/14) 687 GTCCC BstF5I 156 CATCC GGATG(2/0) 219 CATCC DdeI 176 CTGAG C^T[ACGT]AG 567 CTCAG DpnII 70 GATC ^GATC 90 GATC Eco47III 455 AGCGCT AGC^GCT 518 AGCGCT FauI 689 CCCGC CCCGC(4/6) 333 GCGGG FokI 156 CATCC GGATG(9/13) 219 CATCC HinfI 32 GACTC G^A[ACGT]TC 115 GAGTC HphI 118 TCACC GGTGA(8/7) 143 TCACC MboI 70 GATC ^GATC 90 GATC NaeI 440 GCCGGC GCC^GGC 524 GCCGGC NdeII 70 GATC ^GATC 90 GATC NgoMIV 440 GCCGGC G^CCGGC 524 GCCGGC NlaIII 12 CATG CATG^ 112 CATG PleI 32 GACTC GAGTC(4/5) 115 GAGTC PstI 133 CTGCAG CTGCA^G 589 CTGCAG PvuII 73 CAGCTG CAG^CTG 445 CAGCTG RsaI 22 GTAC GT^AC 181 GTAC Sau3AI 70 GATC ^GATC 90 GATC SfcI 133 CTGCAG C^T[GA][CT]AG 589 CTGCAG TspRI 223 CACTG CA[GC]TG[ACGT][ACGT]^ 259 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 43 enzymes) # ######################################### AccII AciI AluI BbvI BcnI BmyI BsaJI BseRI BsiYI BslI Bsp1286I BsrFI BssHII BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I EcoRII Fnu4HI HaeII HaeIII HapII HhaI HinP1I HpaII ItaI MaeIII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIV Sau96I ScrFI SfaNI TseI Tsp45I