(Linear) (Six-base) MAPSORT of: KIEE5278.seq Check: 6560 from: 1 to: 705 KIEE5278.orf With 182 enzymes: SgfI * January 29, 2014 11:53 .. Acc65I G'GTAC_C Cuts at: 0 180 705 Size: 180 525 AfeI AGC'GCT Cuts at: 0 457 520 705 Size: 457 63 185 Fragments arranged by size: 457 185 63 AleI CACnn'nnGTG Cuts at: 0 292 705 Size: 292 413 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 281 705 Size: 281 424 AlwNI CAG_nnn'CTG Cuts at: 0 129 597 705 Size: 129 468 108 Fragments arranged by size: 468 129 108 ApaLI G'TGCA_C Cuts at: 0 256 705 Size: 256 449 AvaI C'yCGr_G Cuts at: 0 614 705 Size: 614 91 BanI G'GyrC_C Cuts at: 0 180 600 705 Size: 180 420 105 Fragments arranged by size: 420 180 105 BanII G_rGCy'C Cuts at: 0 472 535 705 Size: 472 63 170 Fragments arranged by size: 472 170 63 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 144 178 705 Size: 144 34 527 Fragments arranged by size: 527 144 34 BglI GCCn_nnn'nGGC Cuts at: 0 355 530 705 Size: 355 175 175 Fragments arranged by size: 355 175 175 Bme1580I G_kGCm'C Cuts at: 0 260 705 Size: 260 445 BmgBI CAC'GTC Cuts at: 0 339 705 Size: 339 366 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 469 705 Size: 469 236 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 466 499 502 540 705 Size: 466 33 3 38 165 Fragments arranged by size: 466 165 38 33 3 BseYI C'CCAG_C Cuts at: 0 75 122 416 705 Size: 75 47 294 289 Fragments arranged by size: 294 289 75 47 BsiEI CG_ry'CG Cuts at: 0 164 631 705 Size: 164 467 74 Fragments arranged by size: 467 164 74 BsiHKAI G_wGCw'C Cuts at: 0 260 705 Size: 260 445 Bsp1286I G_dGCh'C Cuts at: 0 260 472 535 705 Size: 260 212 63 170 Fragments arranged by size: 260 212 170 63 BsrDI GCAATG_nn' Cuts at: 0 12 705 Size: 12 693 BsrFI r'CCGG_y Cuts at: 0 440 524 626 705 Size: 440 84 102 79 Fragments arranged by size: 440 102 84 79 BssHII G'CGCG_C Cuts at: 0 495 497 646 705 Size: 495 2 149 59 Fragments arranged by size: 495 149 59 2 BstAPI GCAn_nnn'nTGC Cuts at: 0 521 705 Size: 521 184 BstXI CCAn_nnnn'nTGG Cuts at: 0 66 705 Size: 66 639 Bsu36I CC'TnA_GG Cuts at: 0 176 705 Size: 176 529 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 354 705 Size: 354 351 BtsI GCAGTG_nn' Cuts at: 0 222 258 705 Size: 222 36 447 Fragments arranged by size: 447 222 36 EaeI y'GGCC_r Cuts at: 0 628 705 Size: 628 77 EagI C'GGCC_G Cuts at: 0 628 705 Size: 628 77 EarI CTCTTCn'nnn_ Cuts at: 0 401 705 Size: 401 304 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 543 705 Size: 543 162 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 469 705 Size: 469 236 EcoRV GAT'ATC Cuts at: 0 141 705 Size: 141 564 HaeII r_GCGC'y Cuts at: 0 459 522 597 705 Size: 459 63 75 108 Fragments arranged by size: 459 108 75 63 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 23 55 705 Size: 23 32 650 Fragments arranged by size: 650 32 23 HindIII A'AGCT_T Cuts at: 0 433 705 Size: 433 272 KpnI G_GTAC'C Cuts at: 0 184 705 Size: 184 521 MslI CAynn'nnrTG Cuts at: 0 292 705 Size: 292 413 MspA1I CmG'CkG Cuts at: 0 75 447 510 633 639 705 Size: 75 372 63 123 6 66 Fragments arranged by size: 372 123 75 66 63 6 NaeI GCC'GGC Cuts at: 0 442 526 705 Size: 442 84 179 Fragments arranged by size: 442 179 84 NgoMIV G'CCGG_C Cuts at: 0 440 524 705 Size: 440 84 181 Fragments arranged by size: 440 181 84 PfoI T'CCnGG_A Cuts at: 0 27 705 Size: 27 678 PstI C_TGCA'G Cuts at: 0 137 593 705 Size: 137 456 112 Fragments arranged by size: 456 137 112 PvuII CAG'CTG Cuts at: 0 75 447 705 Size: 75 372 258 Fragments arranged by size: 372 258 75 SfcI C'TryA_G Cuts at: 0 133 589 705 Size: 133 456 116 Fragments arranged by size: 456 133 116 SmaI CCC'GGG Cuts at: 0 616 705 Size: 616 89 StyI C'CwwG_G Cuts at: 0 17 705 Size: 17 688 XmaI C'CCGG_G Cuts at: 0 614 705 Size: 614 91 Enzymes that do cut: Acc65I AfeI AleI AlfI AlwNI ApaLI AvaI BanI BanII BcgI BglI Bme1580I BmgBI BpmI BseRI BseYI BsiEI BsiHKAI Bsp1286I BsrDI BsrFI BssHII BstAPI BstXI Bsu36I BtgZI BtsI EaeI EagI EarI EciI Eco57MI EcoRV HaeII Hin4I HindIII KpnI MslI MspA1I NaeI NgoMIV PfoI PstI PvuII SfcI SmaI StyI XmaI Enzymes that do not cut: AarI AatII AccI AclI AcuI AflII AflIII AgeI AhdI AloI ApaI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BciVI BclI BfrBI BglII BlpI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BsgI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrGI BssSI BstBI BstEII BstYI BstZ17I BtgI ClaI CspCI DraI DraIII DrdI EcoICRI EcoNI EcoO109I EcoRI FalI FseI FspI FspAI HincII HpaI KasI MfeI MluI MmeI MscI NarI NcoI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI