################################## # NO CUT ENZYMES ( 118 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AlwNI AocI ApoI AscI AseI AsnI Asp718I AviII BalI BamHI BbrPI BbvCI BclI BfrI BglII BlpI Bpu1102I BsaAI BsaBI BseAI BsgI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI Eco81I EcoNI EcoO65I EcoRI EcoRV FbaI FseI FspI HincII HindII HindIII HpaI KpnI KspI MaeIII MamI MfeI MluI MluNI MroI MscI MseI MunI NdeI NheI NruI NspI NspV PacI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfuI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp45I Tsp509I Van91I XbaI XcmI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AcyI 132 GGCGCC G[GA]^CG[CT]C AhdI 430 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1015 GTGCAC G^TGCAC Aor51HI 671 AGCGCT AGC^GCT ApaLI 1015 GTGCAC G^TGCAC Asp700I 540 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 430 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 406 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 67 CCTAGG C^CTAGG BbeI 132 GGCGCC GGCGC^C BciVI 620 GGATAC GTATCC(6/5) BlnI 67 CCTAGG C^CTAGG BsaHI 132 GGCGCC G[GA]^CG[CT]C BsaI 1003 GAGACC GGTCTC(1/5) BsaWI 617 ACCGGA [AT]^CCGG[AT] BseRI 250 CTCCTC GAGGAG(10/8) BsmBI 411 CGTCTC CGTCTC(1/5) BsmI 544 GCATTC GAATGC(1/-1) BspMI 104 ACCTGC ACCTGC(4/8) BsrBI 284 CCGCTC CCGCTC(-3/-3) BssHII 791 GCGCGC G^CGCGC BstXI 1052 CCACGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 154 GGATCT [GA]^GATC[CT] Eam1105I 430 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 529 GAAGAG CTCTTC(1/4) Eco47III 671 AGCGCT AGC^GCT EcoT22I 811 ATGCAT ATGCA^T HgaI 410 GCGTC GACGC(5/10) Hin1I 132 GGCGCC G[GA]^CG[CT]C HphI 519 TCACC GGTGA(8/7) KasI 132 GGCGCC G^GCGCC Ksp632I 529 GAAGAG CTCTTC(1/4) MflI 154 GGATCT [GA]^GATC[CT] MslI 825 CATGGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 132 GGCGCC GG^CGCC NotI 280 GCGGCCGC GC^GGCCGC NsiI 811 ATGCAT ATGCA^T PaeR7I 287 CTCGAG C^TCGAG PflFI 406 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PvuII 523 CAGCTG CAG^CTG SacI 604 GAGCTC GAGCT^C SfoI 132 GGCGCC GGC^GCC SgrAI 275 CGCCGGCG C[GA]^CCGG[CT]G TaqI 288 TCGA T^CGA Tth111I 406 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 288 TCGA T^CGA XhoI 287 CTCGAG C^TCGAG XhoII 154 GGATCT [GA]^GATC[CT] XmnI 540 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AfaI 702 GTAC GT^AC 711 GTAC AlwI 154 GGATC GGATC(4/5) 919 GGATC AvaII 102 GGACC G^G[AT]CC 169 GGTCC BanI 51 GGTGCC G^G[CT][GA]CC 132 GGCGCC BbsI 442 GAAGAC GAAGAC(2/6) 613 GAAGAC BcgI 289 CGAGCACCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 342 CGAGCCCGCTGC BpuAI 442 GAAGAC GAAGAC(2/6) 613 GAAGAC BsmAI 1003 GAGAC GTCTC(1/5) 412 GTCTC BsmFI 11 GGGAC GGGAC(10/14) 240 GGGAC BsrI 1059 ACTGG ACTGG(1/-1) 435 CCAGT DpnII 155 GATC ^GATC 920 GATC MaeII 314 ACGT A^CGT 418 ACGT MboI 155 GATC ^GATC 920 GATC NaeI 129 GCCGGC GCC^GGC 276 GCCGGC NcoI 719 CCATGG C^CATGG 824 CCATGG NdeII 155 GATC ^GATC 920 GATC NgoMIV 129 GCCGGC G^CCGGC 276 GCCGGC NlaIII 720 CATG CATG^ 825 CATG RsaI 702 GTAC GT^AC 711 GTAC Sau3AI 155 GATC ^GATC 920 GATC SmlI 287 CTCGAG C^T[CT][GA]AG 1034 CTCAAG ######################################### # 3 OR MORE CUT ENZYMES ( 70 enzymes) # ######################################### AccII AciI AluI ApaI AspHI AvaI BanII BbvI BcnI BfaI BglI BmyI BpmI BsaJI BsiEI BsiHKAI BsiYI BslI BsoBI Bsp1286I BsrFI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MaeI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIV PleI Sau96I ScrFI SfaNI SmaI StyI TseI TspRI XmaCI XmaI