(Circular) (Six-base) MAPSORT of: pFN21AB7931.seq Check: 317 from: 1 to: 5800 pFN21AB7931 5800 bp With 182 enzymes: SgfI * January 30, 2014 17:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3610 278 Size: 53 83 186 936 2074 2468 Fragments arranged by size: 2468 2074 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3143 1937 Size: 1206 4594 Acc65I G'GTAC_C Cuts at: 3121 3121 Size: 5800 AclI AA'CG_TT Cuts at: 1563 3927 4300 1563 Size: 2364 373 3063 Fragments arranged by size: 3063 2364 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2466 3863 5247 5515 1632 Size: 834 1397 1384 268 1917 Fragments arranged by size: 1917 1397 1384 834 268 AfeI AGC'GCT Cuts at: 2678 2678 Size: 5800 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5603 AflIII A'CryG_T Cuts at: 1203 4699 5676 1203 Size: 3496 977 1327 Fragments arranged by size: 3496 1327 977 AgeI A'CCGG_T Cuts at: 4631 4631 Size: 5800 AhdI GACnn_n'nnGTC Cuts at: 2440 4529 2440 Size: 2089 3711 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5800 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2762 1184 Size: 1578 4222 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2309 2341 2309 Size: 32 5768 AlwNI CAG_nnn'CTG Cuts at: 3543 5115 3543 Size: 1572 4228 ApaI G_GGCC'C Cuts at: 2201 2236 2345 3075 2201 Size: 35 109 730 4926 Fragments arranged by size: 4926 730 109 35 ApaLI G'TGCA_C Cuts at: 3020 3857 5013 3020 Size: 837 1156 3807 Fragments arranged by size: 3807 1156 837 ApoI r'AATT_y Cuts at: 1493 3109 3335 3487 5596 1493 Size: 1616 226 152 2109 1697 Fragments arranged by size: 2109 1697 1616 226 152 AseI AT'TA_AT Cuts at: 160 1032 4354 160 Size: 872 3322 1606 Fragments arranged by size: 3322 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5800 AvaI C'yCGr_G Cuts at: 1870 1943 2088 2194 2200 2292 2345 2583 3125 Size: 73 145 106 6 92 53 238 542 Cuts at: 3125 1870 Size: 4545 Fragments arranged by size: 4545 542 238 145 106 92 73 53 6 AvrII C'CTAG_G Cuts at: 2072 2072 Size: 5800 BamHI G'GATC_C Cuts at: 3130 3497 3130 Size: 367 5433 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2056 2137 3121 4576 618 Size: 325 217 627 269 81 984 1455 1842 Fragments arranged by size: 1842 1455 984 627 325 269 217 81 BanII G_rGCy'C Cuts at: 1391 2081 2201 2236 2345 2352 2613 2792 2885 Size: 690 120 35 109 7 261 179 93 Cuts at: 2885 2954 3075 3119 1391 Size: 69 121 44 4072 Fragments arranged by size: 4072 690 261 179 121 120 109 93 69 44 35 7 BbeI G_GCGC'C Cuts at: 2141 2141 Size: 5800 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2454 2625 928 Size: 971 555 171 4103 Fragments arranged by size: 4103 971 555 171 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2283 2317 2336 2370 3991 4025 1903 Size: 34 346 34 19 34 1621 34 3678 Fragments arranged by size: 3678 1621 346 34 34 34 34 19 BciVI GTATCCnnnnn_n' Cuts at: 2619 3692 4902 2619 Size: 1073 1210 3517 Fragments arranged by size: 3517 1210 1073 BclI T'GATC_A Cuts at: 1548 1548 Size: 5800 BfrBI ATG'CAT Cuts at: 2818 2818 Size: 5800 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2091 2363 2787 Size: 107 122 71 631 605 419 272 424 Cuts at: 2787 4411 136 Size: 1624 1525 Fragments arranged by size: 1624 1525 631 605 424 419 272 122 107 71 BglII A'GATC_T Cuts at: 5795 5795 Size: 5800 BlpI GC'TnA_GC Cuts at: 3217 3217 Size: 5800 Bme1580I G_kGCm'C Cuts at: 1790 2195 2201 2236 2345 3024 3075 3861 5017 Size: 405 6 35 109 679 51 786 1156 Cuts at: 5017 1790 Size: 2573 Fragments arranged by size: 2573 1156 786 679 405 109 51 35 6 BmgBI CAC'GTC Cuts at: 1318 2320 2424 1318 Size: 1002 104 4694 Fragments arranged by size: 4694 1002 104 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3531 4489 442 Size: 416 53 83 399 24 2114 958 1753 Fragments arranged by size: 2114 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5800 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2200 2974 3086 4460 1239 Size: 961 774 112 1374 2579 Fragments arranged by size: 2579 1374 961 774 112 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3024 3925 4790 5088 3024 Size: 901 865 298 3736 Fragments arranged by size: 3736 901 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3002 4463 882 Size: 617 1503 1461 2219 Fragments arranged by size: 2219 1503 1461 617 BsaAI yAC'GTr Cuts at: 493 1204 3591 493 Size: 711 2387 2702 Fragments arranged by size: 2702 2387 711 BsaBI GATnn'nnATC Cuts at: 5794 5794 Size: 5800 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2138 3607 3989 4624 Size: 53 83 186 936 605 1469 382 635 Cuts at: 4624 275 Size: 1451 Fragments arranged by size: 1469 1451 936 635 605 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2622 4233 4631 4905 5052 5463 2622 Size: 1611 398 274 147 411 2959 Fragments arranged by size: 2959 1611 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2238 2268 2402 2432 3084 Size: 30 165 30 835 30 134 30 652 Cuts at: 3084 3114 1178 Size: 30 3864 Fragments arranged by size: 3864 835 652 165 134 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2246 1186 Size: 1060 4740 BseYI C'CCAG_C Cuts at: 2113 2242 2369 2679 2785 3075 5003 5499 2113 Size: 129 127 310 106 290 1928 496 2414 Fragments arranged by size: 2414 1928 496 310 290 129 127 106 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5227 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2014 2289 2395 3250 4011 5039 Size: 275 186 13 275 106 855 761 1028 Cuts at: 5039 1540 Size: 2301 Fragments arranged by size: 2301 1028 855 761 275 275 186 106 13 BsiHKAI G_wGCw'C Cuts at: 2299 2613 2763 3024 3040 3861 3946 5017 2299 Size: 314 150 261 16 821 85 1071 3082 Fragments arranged by size: 3082 1071 821 314 261 150 85 16 BsmI GAATG_Cn' Cuts at: 2549 3311 3404 2549 Size: 762 93 4945 Fragments arranged by size: 4945 762 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2422 2422 Size: 5800 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2081 2195 2201 2236 2299 2345 2352 Size: 399 291 114 6 35 63 46 7 Cuts at: 2352 2613 2763 2792 2885 2954 3024 3040 3075 Size: 261 150 29 93 69 70 16 35 Cuts at: 3075 3119 3861 3946 5017 1391 Size: 44 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 291 261 150 114 93 85 70 69 63 46 44 35 35 29 16 7 6 BspEI T'CCGG_A Cuts at: 5463 5463 Size: 5800 BspHI T'CATG_A Cuts at: 3689 5419 3689 Size: 1730 4070 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2118 3155 844 Size: 1274 1037 3489 Fragments arranged by size: 3489 1274 1037 BsrBI CCG'CTC Cuts at: 1394 1823 2291 3687 1394 Size: 429 468 1396 3507 Fragments arranged by size: 3507 1396 468 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4295 4469 65 Size: 1183 3047 174 1396 Fragments arranged by size: 3047 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2009 2134 2281 4444 4631 1700 Size: 110 199 125 147 2163 187 2869 Fragments arranged by size: 2869 2163 199 187 147 125 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5800 BssHII G'CGCG_C Cuts at: 1925 2796 1925 Size: 871 4929 BssSI C'ACGA_G Cuts at: 3860 4872 3860 Size: 1012 4788 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5800 BstBI TT'CG_AA Cuts at: 4604 4604 Size: 5800 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5800 BstXI CCAn_nnnn'nTGG Cuts at: 3064 3064 Size: 5800 BstYI r'GATC_y Cuts at: 1972 2159 3130 3497 3882 3899 5351 5437 5449 Size: 187 971 367 385 17 1452 86 12 Cuts at: 5449 5795 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 971 385 367 346 187 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2724 2829 3057 513 Size: 552 1659 105 228 3256 Fragments arranged by size: 3256 1659 552 228 105 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5061 Fragments arranged by size: 5061 683 56 BtsI GCAGTG_nn' Cuts at: 2517 2734 3316 4110 4130 2517 Size: 217 582 794 20 4187 Fragments arranged by size: 4187 794 582 217 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5800 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5765 DraI TTT'AAA Cuts at: 3104 3104 Size: 5800 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4807 809 Size: 507 3491 1802 Fragments arranged by size: 3491 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2011 2286 2392 Size: 54 1425 50 275 18 181 275 106 Cuts at: 2392 2700 3247 4136 5765 8 Size: 308 547 889 1629 43 Fragments arranged by size: 1629 1425 889 547 308 275 275 181 106 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2011 2286 2392 3247 1537 Size: 275 199 275 106 855 4090 Fragments arranged by size: 4090 855 275 275 199 106 EarI CTCTTCn'nnn_ Cuts at: 1353 2529 3730 1353 Size: 1176 1201 3423 Fragments arranged by size: 3423 1201 1176 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2367 2758 4387 4761 4907 387 Size: 1064 138 778 391 1629 374 146 1280 Fragments arranged by size: 1629 1280 1064 778 391 374 146 138 EcoICRI GAG'CTC Cuts at: 2611 2611 Size: 5800 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2200 2466 2974 3086 3863 4460 5247 Size: 393 568 266 508 112 777 597 787 Cuts at: 5247 5515 1239 Size: 268 1524 Fragments arranged by size: 1524 787 777 597 568 508 393 268 266 112 EcoO109I rG'GnC_Cy Cuts at: 2232 2233 2341 2342 3071 3072 2232 Size: 1 108 1 729 1 4960 Fragments arranged by size: 4960 729 108 1 1 1 EcoRI G'AATT_C Cuts at: 3109 3487 3109 Size: 378 5422 FspI TGC'GCA Cuts at: 4306 4306 Size: 5800 HaeII r_GCGC'y Cuts at: 1678 2054 2141 2680 4947 1678 Size: 376 87 539 2267 2531 Fragments arranged by size: 2531 2267 539 376 87 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2238 2270 2402 2426 2434 Size: 32 135 32 666 32 132 24 8 Cuts at: 2434 2458 2909 2941 4441 4473 4515 4547 1373 Size: 24 451 32 1500 32 42 32 2626 Fragments arranged by size: 2626 1500 666 451 135 132 42 32 32 32 32 32 32 24 24 8 HincII GTy'rAC Cuts at: 669 1657 1938 3144 3390 669 Size: 988 281 1206 246 3079 Fragments arranged by size: 3079 1206 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5800 HpaI GTT'AAC Cuts at: 3390 3390 Size: 5800 KasI G'GCGC_C Cuts at: 2137 2137 Size: 5800 KpnI G_GTAC'C Cuts at: 3125 3125 Size: 5800 MfeI C'AATT_G Cuts at: 3399 3399 Size: 5800 MluI A'CGCG_T Cuts at: 5676 5676 Size: 5800 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2052 2520 3075 4914 5098 1305 Size: 747 468 555 1839 184 2007 Fragments arranged by size: 2007 1839 747 555 468 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3978 Fragments arranged by size: 3978 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2834 3758 4117 4276 518 Size: 648 1668 924 359 159 2042 Fragments arranged by size: 2042 1668 924 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2144 2242 2354 2362 2530 2746 3216 3893 Size: 538 98 112 8 168 216 470 677 Cuts at: 3893 5041 5286 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 538 470 245 216 168 112 98 8 NaeI GCC'GGC Cuts at: 2136 2283 2136 Size: 147 5653 NarI GG'CG_CC Cuts at: 2138 2138 Size: 5800 NcoI C'CATG_G Cuts at: 513 1065 2724 2829 513 Size: 552 1659 105 3484 Fragments arranged by size: 3484 1659 552 105 NdeI CA'TA_TG Cuts at: 387 387 Size: 5800 NgoMIV G'CCGG_C Cuts at: 2134 2281 2134 Size: 147 5653 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5800 NotI GC'GGCC_GC Cuts at: 2286 3247 2286 Size: 961 4839 NsiI A_TGCA'T Cuts at: 2820 2820 Size: 5800 NspI r_CATG'y Cuts at: 1132 1229 3158 4703 1132 Size: 97 1929 1545 2229 Fragments arranged by size: 2229 1929 1545 97 PasI CC'CwG_GG Cuts at: 1348 2337 3026 1348 Size: 989 689 4122 Fragments arranged by size: 4122 989 689 PciI A'CATG_T Cuts at: 4699 4699 Size: 5800 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5800 PmeI GTTT'AAAC Cuts at: 3104 3104 Size: 5800 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3869 3901 5382 5414 3869 Size: 32 1481 32 4255 Fragments arranged by size: 4255 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5800 PsiI TTA'TAA Cuts at: 3370 3370 Size: 5800 PspOMI G'GGCC_C Cuts at: 2197 2232 2341 3071 2197 Size: 35 109 730 4926 Fragments arranged by size: 4926 730 109 35 PspXI vC'TCGA_Gb Cuts at: 2292 2292 Size: 5800 PstI C_TGCA'G Cuts at: 830 3152 830 Size: 2322 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5800 PvuII CAG'CTG Cuts at: 2530 2530 Size: 5800 SacI G_AGCT'C Cuts at: 2613 2613 Size: 5800 SalI G'TCGA_C Cuts at: 1936 3142 1936 Size: 1206 4594 SbfI CC_TGCA'GG Cuts at: 3152 3152 Size: 5800 ScaI AGT'ACT Cuts at: 4048 5760 4048 Size: 1712 4088 SfcI C'TryA_G Cuts at: 826 1045 3148 4283 4964 5155 826 Size: 219 2103 1135 681 191 1471 Fragments arranged by size: 2103 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2139 2139 Size: 5800 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5800 SgrAI Cr'CCGG_yG Cuts at: 1700 2281 1700 Size: 581 5219 SmaI CCC'GGG Cuts at: 1872 2090 2196 2202 3127 1872 Size: 218 106 6 925 4545 Fragments arranged by size: 4545 925 218 106 6 SmlI C'TyrA_G Cuts at: 820 1017 1943 2292 3039 3904 4805 5067 820 Size: 197 926 349 747 865 901 262 1553 Fragments arranged by size: 1553 926 901 865 747 349 262 197 SnaBI TAC'GTA Cuts at: 493 3591 493 Size: 3098 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5800 SphI G_CATG'C Cuts at: 1132 3158 1132 Size: 2026 3774 SrfI GCCC'GGGC Cuts at: 2196 2196 Size: 5800 SspI AAT'ATT Cuts at: 5 52 3724 5 Size: 47 3672 2081 Fragments arranged by size: 3672 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5800 StyI C'CwwG_G Cuts at: 513 1065 2072 2478 2724 2829 3239 513 Size: 552 1007 406 246 105 410 3074 Fragments arranged by size: 3074 1007 552 410 406 246 105 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3827 3844 3997 4182 4627 325 Size: 1255 2247 17 153 185 445 1498 Fragments arranged by size: 2247 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4046 5758 96 Size: 274 80 33 51 1443 2069 1712 138 Fragments arranged by size: 2069 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5760 5792 5760 Size: 32 5768 Tth111I GACn'n_nGTC Cuts at: 1316 2414 1316 Size: 1098 4702 XbaI T'CTAG_A Cuts at: 3136 3136 Size: 5800 XhoI C'TCGA_G Cuts at: 1943 2292 1943 Size: 349 5451 XmaI C'CCGG_G Cuts at: 1870 2088 2194 2200 3125 1870 Size: 218 106 6 925 4545 Fragments arranged by size: 4545 925 218 106 6 XmnI GAAnn'nnTTC Cuts at: 2549 3929 2549 Size: 1380 4420 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3608 276 Size: 53 83 186 936 2074 2468 Fragments arranged by size: 2468 2074 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI BaeI BbvCI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NruI PacI PfoI PmlI PpuMI PsrI RsrII SacII SanDI SapI SexAI SfiI SwaI XcmI