(Linear) (Six-base) MAPSORT of: KIBB7931.seq Check: 7007 from: 1 to: 1095 KIBB7931.orf With 182 enzymes: SgfI * January 29, 2014 11:43 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 461 1095 Size: 461 634 AfeI AGC'GCT Cuts at: 0 673 1095 Size: 673 422 AhdI GACnn_n'nnGTC Cuts at: 0 435 1095 Size: 435 660 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 757 1095 Size: 757 338 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 304 336 1095 Size: 304 32 759 Fragments arranged by size: 759 304 32 ApaI G_GGCC'C Cuts at: 0 196 231 340 1070 1095 Size: 196 35 109 730 25 Fragments arranged by size: 730 196 109 35 25 ApaLI G'TGCA_C Cuts at: 0 1015 1095 Size: 1015 80 AvaI C'yCGr_G Cuts at: 0 83 189 195 287 340 578 1095 Size: 83 106 6 92 53 238 517 Fragments arranged by size: 517 238 106 92 83 53 6 AvrII C'CTAG_G Cuts at: 0 67 1095 Size: 67 1028 BanI G'GyrC_C Cuts at: 0 51 132 1095 Size: 51 81 963 Fragments arranged by size: 963 81 51 BanII G_rGCy'C Cuts at: 0 76 196 231 340 347 608 787 880 Size: 76 120 35 109 7 261 179 93 Cuts at: 880 949 1070 1095 Size: 69 121 25 Fragments arranged by size: 261 179 121 120 109 93 76 69 35 25 7 BbeI G_GCGC'C Cuts at: 0 136 1095 Size: 136 959 BbsI GAAGACnn'nnnn_ Cuts at: 0 449 620 1095 Size: 449 171 475 Fragments arranged by size: 475 449 171 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 278 312 331 365 1095 Size: 278 34 19 34 730 Fragments arranged by size: 730 278 34 34 19 BciVI GTATCCnnnnn_n' Cuts at: 0 614 1095 Size: 614 481 BfrBI ATG'CAT Cuts at: 0 813 1095 Size: 813 282 BglI GCCn_nnn'nGGC Cuts at: 0 86 358 782 1095 Size: 86 272 424 313 Fragments arranged by size: 424 313 272 86 Bme1580I G_kGCm'C Cuts at: 0 190 196 231 340 1019 1070 1095 Size: 190 6 35 109 679 51 25 Fragments arranged by size: 679 190 109 51 35 25 6 BmgBI CAC'GTC Cuts at: 0 315 419 1095 Size: 315 104 676 Fragments arranged by size: 676 315 104 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 195 969 1081 1095 Size: 195 774 112 14 Fragments arranged by size: 774 195 112 14 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1019 1095 Size: 1019 76 BsaI GGTCTCn'nnnn_ Cuts at: 0 997 1095 Size: 997 98 BsaHI Gr'CG_yC Cuts at: 0 133 1095 Size: 133 962 BsaWI w'CCGG_w Cuts at: 0 617 1095 Size: 617 478 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 233 263 397 427 1095 Size: 233 30 134 30 668 Fragments arranged by size: 668 233 134 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 241 1095 Size: 241 854 BseYI C'CCAG_C Cuts at: 0 108 237 364 674 780 1070 1095 Size: 108 129 127 310 106 290 25 Fragments arranged by size: 310 290 129 127 108 106 25 BsiEI CG_ry'CG Cuts at: 0 9 284 390 1095 Size: 9 275 106 705 Fragments arranged by size: 705 275 106 9 BsiHKAI G_wGCw'C Cuts at: 0 294 608 758 1019 1035 1095 Size: 294 314 150 261 16 60 Fragments arranged by size: 314 294 261 150 60 16 BsmI GAATG_Cn' Cuts at: 0 544 1095 Size: 544 551 BsmBI CGTCTCn'nnnn_ Cuts at: 0 417 1095 Size: 417 678 Bsp1286I G_dGCh'C Cuts at: 0 76 190 196 231 294 340 347 608 Size: 76 114 6 35 63 46 7 261 Cuts at: 608 758 787 880 949 1019 1035 1070 1095 Size: 150 29 93 69 70 16 35 25 Fragments arranged by size: 261 150 114 93 76 70 69 63 46 35 35 29 25 16 7 6 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 113 1095 Size: 113 982 BsrBI CCG'CTC Cuts at: 0 286 1095 Size: 286 809 BsrFI r'CCGG_y Cuts at: 0 4 129 276 1095 Size: 4 125 147 819 Fragments arranged by size: 819 147 125 4 BssHII G'CGCG_C Cuts at: 0 791 1095 Size: 791 304 BstXI CCAn_nnnn'nTGG Cuts at: 0 1059 1095 Size: 1059 36 BstYI r'GATC_y Cuts at: 0 154 1095 Size: 154 941 BtgI C'CryG_G Cuts at: 0 719 824 1052 1095 Size: 719 105 228 43 Fragments arranged by size: 719 228 105 43 BtsI GCAGTG_nn' Cuts at: 0 512 729 1095 Size: 512 217 366 Fragments arranged by size: 512 366 217 EaeI y'GGCC_r Cuts at: 0 6 281 387 695 1095 Size: 6 275 106 308 400 Fragments arranged by size: 400 308 275 106 6 EagI C'GGCC_G Cuts at: 0 6 281 387 1095 Size: 6 275 106 708 Fragments arranged by size: 708 275 106 6 EarI CTCTTCn'nnn_ Cuts at: 0 524 1095 Size: 524 571 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 362 753 1095 Size: 362 391 342 Fragments arranged by size: 391 362 342 EcoICRI GAG'CTC Cuts at: 0 606 1095 Size: 606 489 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 195 461 969 1081 1095 Size: 195 266 508 112 14 Fragments arranged by size: 508 266 195 112 14 EcoO109I rG'GnC_Cy Cuts at: 0 227 228 336 337 1066 1067 1095 Size: 227 1 108 1 729 1 28 Fragments arranged by size: 729 227 108 28 1 1 1 HaeII r_GCGC'y Cuts at: 0 49 136 675 1095 Size: 49 87 539 420 Fragments arranged by size: 539 420 87 49 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 233 265 397 421 429 453 904 936 Size: 233 32 132 24 8 24 451 32 Cuts at: 936 1095 Size: 159 Fragments arranged by size: 451 233 159 132 32 32 24 24 8 KasI G'GCGC_C Cuts at: 0 132 1095 Size: 132 963 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 47 515 1070 1095 Size: 47 468 555 25 Fragments arranged by size: 555 468 47 25 MslI CAynn'nnrTG Cuts at: 0 829 1095 Size: 829 266 MspA1I CmG'CkG Cuts at: 0 139 237 349 357 525 741 1095 Size: 139 98 112 8 168 216 354 Fragments arranged by size: 354 216 168 139 112 98 8 NaeI GCC'GGC Cuts at: 0 131 278 1095 Size: 131 147 817 Fragments arranged by size: 817 147 131 NarI GG'CG_CC Cuts at: 0 133 1095 Size: 133 962 NcoI C'CATG_G Cuts at: 0 719 824 1095 Size: 719 105 271 Fragments arranged by size: 719 271 105 NgoMIV G'CCGG_C Cuts at: 0 129 276 1095 Size: 129 147 819 Fragments arranged by size: 819 147 129 NotI GC'GGCC_GC Cuts at: 0 281 1095 Size: 281 814 NsiI A_TGCA'T Cuts at: 0 815 1095 Size: 815 280 PasI CC'CwG_GG Cuts at: 0 332 1021 1095 Size: 332 689 74 Fragments arranged by size: 689 332 74 PspOMI G'GGCC_C Cuts at: 0 192 227 336 1066 1095 Size: 192 35 109 730 29 Fragments arranged by size: 730 192 109 35 29 PspXI vC'TCGA_Gb Cuts at: 0 287 1095 Size: 287 808 PvuII CAG'CTG Cuts at: 0 525 1095 Size: 525 570 SacI G_AGCT'C Cuts at: 0 608 1095 Size: 608 487 SfoI GGC'GCC Cuts at: 0 134 1095 Size: 134 961 SgrAI Cr'CCGG_yG Cuts at: 0 276 1095 Size: 276 819 SmaI CCC'GGG Cuts at: 0 85 191 197 1095 Size: 85 106 6 898 Fragments arranged by size: 898 106 85 6 SmlI C'TyrA_G Cuts at: 0 287 1034 1095 Size: 287 747 61 Fragments arranged by size: 747 287 61 SrfI GCCC'GGGC Cuts at: 0 191 1095 Size: 191 904 StyI C'CwwG_G Cuts at: 0 67 473 719 824 1095 Size: 67 406 246 105 271 Fragments arranged by size: 406 271 246 105 67 Tth111I GACn'n_nGTC Cuts at: 0 409 1095 Size: 409 686 XhoI C'TCGA_G Cuts at: 0 287 1095 Size: 287 808 XmaI C'CCGG_G Cuts at: 0 83 189 195 1095 Size: 83 106 6 900 Fragments arranged by size: 900 106 83 6 XmnI GAAnn'nnTTC Cuts at: 0 544 1095 Size: 544 551 Enzymes that do cut: AcuI AfeI AhdI AlfI AloI ApaI ApaLI AvaI AvrII BanI BanII BbeI BbsI BcgI BciVI BfrBI BglI Bme1580I BmgBI BpmI BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII BstXI BstYI BtgI BtsI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I KasI MmeI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NsiI PasI PspOMI PspXI PvuII SacI SfoI SgrAI SmaI SmlI SrfI StyI Tth111I XhoI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AflIII AgeI AleI AlwNI ApoI AscI AseI AsiSI BaeI BamHI BbvCI BclI BglII BlpI BmrI BmtI BplI Bpu10I BsaAI BsaBI BsgI BsiWI BspEI BspHI BsrDI BsrGI BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MscI NdeI NheI NruI NspI PacI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SnaBI SpeI SphI SspI StuI SwaI TaqII TatI TstI XbaI XcmI ZraI