################################## # NO CUT ENZYMES ( 186 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbeI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DpnII DraI DraIII DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HapII HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII HphI KasI KpnI Ksp632I KspI MamI MboI MboII MfeI MflI MluI MluNI MroI MscI MslI MspA1I MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TfiI Tth111I Van91I XbaI XcmI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AluI 367 AGCT AG^CT AspHI 45 GAGCAC G[AT]GC[AT]^C AvaI 268 CTCGAG C^[CT]CG[GA]G AvaII 84 GGACC G^G[AT]CC BanI 414 GGTGCC G^G[CT][GA]CC BbrPI 95 CACGTG CAC^GTG BfaI 235 CTAG C^TAG BpmI 400 CTCCAG CTGGAG(16/14) BsaAI 95 CACGTG [CT]AC^GT[GA] BsgI 186 CTGCAC GTGCAG(16/14) BsiHKAI 45 GAGCAC G[AT]GC[AT]^C BsiYI 312 CCTGCGTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 312 CCTGCGTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 216 GTCCC GGGAC(10/14) BsoBI 268 CTCGAG C^[CT]CG[GA]G BspLU11I 197 ACATGT A^CATGT BsrBI 5 GAGCGG CCGCTC(-3/-3) BsrI 52 ACTGG ACTGG(1/-1) BstF5I 321 GGATG GGATG(2/0) DdeI 364 CTGAG C^T[ACGT]AG DrdI 136 GACCCAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FauI 218 CCCGC CCCGC(4/6) FokI 321 GGATG GGATG(9/13) HgaI 315 GCGTC GACGC(5/10) MaeI 235 CTAG C^TAG MaeII 96 ACGT A^CGT MseI 89 TTAA T^TAA NlaIII 198 CATG CATG^ NspI 197 ACATGT [GA]CATG^[CT] PaeR7I 268 CTCGAG C^TCGAG PmaCI 95 CACGTG CAC^GTG PmlI 95 CACGTG CAC^GTG PpuMI 83 AGGACCT [GA]G^G[AT]CC[CT] SfaNI 221 GCATC GCATC(5/9) StuI 252 AGGCCT AGG^CCT Tru9I 89 TTAA T^TAA Tsp509I 279 AATT ^AATT XhoI 268 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AciI 219 CCGC CCGC(-3/-1) 7 GCGG AflIII 96 ACGTGT A^C[GA][CT]GT 197 ACATGT AlwNI 307 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG 358 CAGACTCTG ApaI 57 GGGCCC GGGCC^C 230 GGGCCC BanII 57 GGGCCC G[GA]GC[CT]^C 230 GGGCCC BspMI 292 ACCTGC ACCTGC(4/8) 311 ACCTGC Cac8I 3 GCGAGC GC[ACGT]^[ACGT]GC 21 GCTGGC MaeIII 329 GTGAC ^GT[ACGT]AC 431 GTCAC SmlI 42 CTTGAG C^T[CT][GA]AG 268 CTCGAG TaqI 269 TCGA T^CGA 410 TCGA Tsp45I 329 GTGAC ^GT[GC]AC 431 GTCAC TspRI 51 CACTG CA[GC]TG[ACGT][ACGT]^ 165 CAGTG TthHB8I 269 TCGA T^CGA 410 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 21 enzymes) # ######################################### BbvI BmyI BsaJI Bsp1286I BssKI BstNI Cfr13I DraII EcoO109I EcoRII Fnu4HI HaeIII HinfI ItaI MnlI MvaI NlaIV PleI Sau96I ScrFI TseI