(Linear) (Six-base) MAPSORT of: KIEE1651.seq  Check: 7157  from: 1  to: 435



KIEE1651.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:44  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     320     435 
   Size:        320     115
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     398     435 
   Size:        398      37
 
AflIII A'CryG_T

Cuts at:      0      96     197     435 
   Size:         96     101     238

  Fragments arranged by size: 

                 238     101      96
 
AlwNI CAG_nnn'CTG

Cuts at:      0     312     363     435 
   Size:        312      51      72

  Fragments arranged by size: 

                 312      72      51
 
ApaI G_GGCC'C

Cuts at:      0      61     234     435 
   Size:         61     173     201

  Fragments arranged by size: 

                 201     173      61
 
AvaI C'yCGr_G

Cuts at:      0     268     435 
   Size:        268     167
 
BanI G'GyrC_C

Cuts at:      0     414     435 
   Size:        414      21
 
BanII G_rGCy'C

Cuts at:      0      61     234     435 
   Size:         61     173     201

  Fragments arranged by size: 

                 201     173      61
 
Bme1580I G_kGCm'C

Cuts at:      0      61     234     395     435 
   Size:         61     173     161      40

  Fragments arranged by size: 

                 173     161      61      40
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      62     435 
   Size:         62     373
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     385     435 
   Size:        385      50
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      63     435 
   Size:         63     372
 
BsaAI yAC'GTr

Cuts at:      0      97     435 
   Size:         97     338
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     171     435 
   Size:        171     264
 
BsiHKAI G_wGCw'C

Cuts at:      0      49     435 
   Size:         49     386
 
Bsp1286I G_dGCh'C

Cuts at:      0      49      61     234     395     435 
   Size:         49      12     173     161      40

  Fragments arranged by size: 

                 173     161      49      40      12
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     301     320     435 
   Size:        301      19     115

  Fragments arranged by size: 

                 301     115      19
 
BsrBI CCG'CTC

Cuts at:      0       7     435 
   Size:          7     428
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     142     435 
   Size:        142     293
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     385     398     435 
   Size:        385      13      37

  Fragments arranged by size: 

                 385      37      13
 
EcoO109I rG'GnC_Cy

Cuts at:      0      57      58      84     230     231     435 
   Size:         57       1      26     146       1     204

  Fragments arranged by size: 

                 204     146      57      26       1       1
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      76     108     435 
   Size:         76      32     327

  Fragments arranged by size: 

                 327      76      32
 
NspI r_CATG'y

Cuts at:      0     201     435 
   Size:        201     234
 
PciI A'CATG_T

Cuts at:      0     197     435 
   Size:        197     238
 
PmlI CAC'GTG

Cuts at:      0      97     435 
   Size:         97     338
 
PpuMI rG'GwC_Cy

Cuts at:      0      84     435 
   Size:         84     351
 
PspOMI G'GGCC_C

Cuts at:      0      57     230     435 
   Size:         57     173     205

  Fragments arranged by size: 

                 205     173      57
 
PspXI vC'TCGA_Gb

Cuts at:      0     268     435 
   Size:        268     167
 
SmlI C'TyrA_G

Cuts at:      0      42     268     435 
   Size:         42     226     167

  Fragments arranged by size: 

                 226     167      42
 
StuI AGG'CCT

Cuts at:      0     254     435 
   Size:        254     181
 
XhoI C'TCGA_G

Cuts at:      0     268     435 
   Size:        268     167
 

 Enzymes that do cut:

     AarI     AcuI   AflIII    AlwNI     ApaI     AvaI     BanI    BanII
 Bme1580I     BmrI     BpmI    BpuEI    BsaAI     BsgI  BsiHKAI Bsp1286I
    BspMI    BsrBI     DrdI  Eco57MI EcoO109I    Hin4I     NspI     PciI
     PmlI    PpuMI   PspOMI    PspXI     SmlI     StuI     XhoI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI     BplI
   Bpu10I     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmeI     PpiI
    PshAI     PsiI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StyI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI     XmnI     ZraI