################################## # NO CUT ENZYMES ( 187 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI AluI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BciVI BclI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DdeI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI KasI KpnI KspI MaeII MaeIII MamI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tsp45I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AciI 198 CCGC CCGC(-3/-1) AcsI 392 AAATTT [GA]^AATT[CT] ApoI 392 AAATTT [GA]^AATT[CT] AseI 175 ATTAAT AT^TAAT AsnI 175 ATTAAT AT^TAAT AvaII 83 GGACC G^G[AT]CC BcgI 274 CGAACCACATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 59 CCGGG CC^[GC]GG BfaI 80 CTAG C^TAG BmyI 137 GTGCCC G[AGT]GC[ACT]^C BsgI 130 GTGCAG GTGCAG(16/14) BsiEI 270 CGGTCG CG[GA][CT]^CG Bsp1286I 137 GTGCCC G[AGT]GC[ACT]^C Cac8I 135 GCGTGC GC[ACGT]^[ACGT]GC DraII 82 AGGACCT [GA]G^G[ACGT]CC[CT] DsaI 53 CCGTGG C^C[GA][CT]GG EarI 346 GAAGAG CTCTTC(1/4) EcoO109I 82 AGGACCT [GA]G^G[ACGT]CC[CT] EcoT14I 86 CCTTGG C^C[AT][AT]GG Ksp632I 346 GAAGAG CTCTTC(1/4) MaeI 80 CTAG C^TAG MboII 346 GAAGA GAAGA(8/7) MseI 176 TTAA T^TAA MwoI 58 GCCGGGAGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NciI 59 CCGGG CC^[GC]GG NspI 280 ACATGC [GA]CATG^[CT] PpuMI 82 AGGACCT [GA]G^G[AT]CC[CT] PshAI 307 GACAACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 175 ATTAAT AT^TAAT PstI 205 CTGCAG CTGCA^G ScaI 188 AGTACT AGT^ACT SfaNI 98 GCATC GCATC(5/9) StyI 86 CCTTGG C^C[AT][AT]GG TaqI 273 TCGA T^CGA Tru9I 176 TTAA T^TAA Tsp509I 393 AATT ^AATT TspRI 23 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 273 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AfaI 162 GTAC GT^AC 189 GTAC BsmFI 291 GTCCC GGGAC(10/14) 314 GTCCC BspMI 91 GCAGGT ACCTGC(4/8) 207 GCAGGT BstF5I 99 CATCC GGATG(2/0) 183 GGATG BstNI 141 CCTGG CC^[AT]GG 380 CCTGG DpnII 231 GATC ^GATC 366 GATC EaeI 50 TGGCCG [CT]^GGCC[GA] 56 TGGCCG EcoRII 141 CCTGG ^CC[AT]GG 380 CCTGG FokI 99 CATCC GGATG(9/13) 183 GGATG HapII 59 CCGG C^CGG 417 CCGG HpaII 59 CCGG C^CGG 417 CCGG HphI 210 GGTGA GGTGA(8/7) 243 GGTGA MboI 231 GATC ^GATC 366 GATC MspI 59 CCGG C^CGG 417 CCGG MvaI 141 CCTGG CC^[AT]GG 380 CCTGG NdeII 231 GATC ^GATC 366 GATC RsaI 162 GTAC GT^AC 189 GTAC Sau3AI 231 GATC ^GATC 366 GATC SfcI 192 CTACAG C^T[GA][CT]AG 205 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 14 enzymes) # ######################################### BbvI BsaJI BsiYI BslI BssKI Cfr13I Fnu4HI HaeIII ItaI MnlI NlaIII Sau96I ScrFI TseI