################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiWI BsmBI BsmI BspDI BspEI BspLU11I BsrBI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraIII DrdI Eam1105I Eco47III Eco81I EcoO65I EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HindIII KasI KpnI MaeII MamI MfeI MluI MluNI MroI MscI MslI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SalI SapI SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AccII 106 CGCG CG^CG AlwNI 887 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 506 GAGCAC G[AT]GC[AT]^C AvaI 187 CCCGAG C^[CT]CG[GA]G AvaII 54 GGTCC G^G[AT]CC BanII 16 GGGCTC G[GA]GC[CT]^C BglI 9 GCCGGCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 905 AGATCT A^GATCT BseRI 217 GAGGAG GAGGAG(10/8) BsiHKAI 506 GAGCAC G[AT]GC[AT]^C BsoBI 187 CCCGAG C^[CT]CG[GA]G BspHI 933 TCATGA T^CATGA BspMI 891 ACCTGC ACCTGC(4/8) BsrFI 9 GCCGGC [GA]^CCGG[CT] BsrI 170 ACTGG ACTGG(1/-1) BstAPI 361 GCAGAAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 922 GGATG GGATG(2/0) BstUI 106 CGCG CG^CG CfoI 24 GCGC GCG^C Cfr10I 9 GCCGGC [GA]^CCGG[CT] Cfr13I 54 GGTCC G^G[ACGT]CC DdeI 384 CTCAG C^T[ACGT]AG DraI 531 TTTAAA TTT^AAA DraII 53 GGGTCCC [GA]G^G[ACGT]CC[CT] DsaI 105 CCGCGG C^C[GA][CT]GG EagI 7 CGGCCG C^GGCCG EarI 296 GAAGAG CTCTTC(1/4) EclXI 7 CGGCCG C^GGCCG Eco52I 7 CGGCCG C^GGCCG EcoNI 210 CCTGAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 53 GGGTCCC [GA]G^G[ACGT]CC[CT] EcoRI 427 GAATTC G^AATTC EcoT14I 40 CCTTGG C^C[AT][AT]GG FauI 104 CCCGC CCCGC(4/6) FokI 922 GGATG GGATG(9/13) HaeII 23 GGCGCT [GA]GCGC^[CT] HhaI 24 GCGC GCG^C HinP1I 24 GCGC G^CGC HincII 736 GTTAAC GT[CT]^[GA]AC HindII 736 GTTAAC GT[CT]^[GA]AC HpaI 736 GTTAAC GTT^AAC Ksp632I 296 GAAGAG CTCTTC(1/4) KspI 105 CCGCGG CCGC^GG MvnI 106 CGCG CG^CG NaeI 9 GCCGGC GCC^GGC NgoMIV 9 GCCGGC G^CCGGC PleI 937 GAGTC GAGTC(4/5) PpuMI 53 GGGTCCC [GA]G^G[AT]CC[CT] RcaI 933 TCATGA T^CATGA SacII 105 CCGCGG CCGC^GG Sau96I 54 GGTCC G^G[ACGT]CC SexAI 458 ACCTGGT A^CC[AT]GGT SfaNI 822 GCATC GCATC(5/9) SmlI 511 CTTGAG C^T[CT][GA]AG StyI 40 CCTTGG C^C[AT][AT]GG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AlwI 233 GGATC GGATC(4/5) 282 GGATC Asp700I 132 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 427 GAATTCCTTC BfaI 503 CTAG C^TAG 991 CTAG BmyI 16 GGGCTC G[AGT]GC[ACT]^C 506 GAGCAC BsiEI 7 CGGCCG CG[GA][CT]^CG 141 CGACCG BsiYI 210 CCTGAAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 273 CCTTGCTTTGG BslI 210 CCTGAAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 273 CCTTGCTTTGG BsmAI 827 GAGAC GTCTC(1/5) 97 GTCTC BsmFI 675 GGGAC GGGAC(10/14) 55 GTCCC Bsp1286I 16 GGGCTC G[AGT]GC[ACT]^C 506 GAGCAC Bsp1407I 854 TGTACA T^GTACA 866 TGTACA BsrGI 854 TGTACA T^GTACA 866 TGTACA BstYI 282 GGATCT [GA]^GATC[CT] 905 AGATCT EaeI 7 CGGCCG [CT]^GGCC[GA] 72 TGGCCG HaeIII 8 GGCC GG^CC 73 GGCC MaeI 503 CTAG C^TAG 991 CTAG MflI 282 GGATCT [GA]^GATC[CT] 905 AGATCT NlaIII 915 CATG CATG^ 934 CATG PvuII 842 CAGCTG CAG^CTG 953 CAGCTG SspBI 854 TGTACA T^GTACA 866 TGTACA SspI 133 AATATT AAT^ATT 314 AATATT TaqI 140 TCGA T^CGA 825 TCGA TfiI 66 GAATC G^A[AT]TC 519 GATTC Tsp45I 85 GTCAC ^GT[GC]AC 939 GTCAC TthHB8I 140 TCGA T^CGA 825 TCGA XhoII 282 GGATCT [GA]^GATC[CT] 905 AGATCT XmnI 132 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 427 GAATTCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI BbvI BcnI BsaJI BsrDI BssKI BstNI Cac8I DpnII EcoRII Fnu4HI HapII HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIV RsaI Sau3AI ScrFI Tru9I TseI Tsp509I TspRI