(Circular) (Six-base) MAPSORT of: pFN21AE3135.seq Check: 2267 from: 1 to: 5740 pFN21AE3135 5740 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3550 278 Size: 53 83 186 936 2014 2468 Fragments arranged by size: 2468 2014 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3083 1937 Size: 1146 4594 Acc65I G'GTAC_C Cuts at: 3061 3061 Size: 5740 AclI AA'CG_TT Cuts at: 1563 3867 4240 1563 Size: 2304 373 3063 Fragments arranged by size: 3063 2304 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2237 3803 5187 5455 1632 Size: 605 1566 1384 268 1917 Fragments arranged by size: 1917 1566 1384 605 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5543 AflIII A'CryG_T Cuts at: 1203 4639 5616 1203 Size: 3436 977 1327 Fragments arranged by size: 3436 1327 977 AgeI A'CCGG_T Cuts at: 4571 4571 Size: 5740 AhdI GACnn_n'nnGTC Cuts at: 4469 4469 Size: 5740 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5740 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5740 AlwNI CAG_nnn'CTG Cuts at: 2897 3483 5055 2897 Size: 586 1572 3582 Fragments arranged by size: 3582 1572 586 ApaLI G'TGCA_C Cuts at: 3797 4953 3797 Size: 1156 4584 ApoI r'AATT_y Cuts at: 1493 2204 2269 2383 2432 2733 3049 3275 3427 Size: 711 65 114 49 301 316 226 152 Cuts at: 3427 5536 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 711 316 301 226 152 114 65 49 AseI AT'TA_AT Cuts at: 160 1032 4294 160 Size: 872 3262 1606 Fragments arranged by size: 3262 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5740 AvaI C'yCGr_G Cuts at: 1870 1943 2192 3065 1870 Size: 73 249 873 4545 Fragments arranged by size: 4545 873 249 73 BamHI G'GATC_C Cuts at: 3070 3437 3070 Size: 367 5373 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3061 4516 618 Size: 325 217 627 1274 1455 1842 Fragments arranged by size: 1842 1455 1274 627 325 217 BanII G_rGCy'C Cuts at: 1391 2025 3059 1391 Size: 634 1034 4072 Fragments arranged by size: 4072 1034 634 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4769 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3931 3965 1903 Size: 34 50 34 1910 34 3678 Fragments arranged by size: 3678 1910 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3632 4842 3632 Size: 1210 4530 BclI T'GATC_A Cuts at: 1548 1548 Size: 5740 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2020 4351 136 Size: 107 122 71 631 605 348 2331 1525 Fragments arranged by size: 2331 1525 631 605 348 122 107 71 BglII A'GATC_T Cuts at: 2910 5735 2910 Size: 2825 2915 BlpI GC'TnA_GC Cuts at: 3157 3157 Size: 5740 Bme1580I G_kGCm'C Cuts at: 1790 3801 4957 1790 Size: 2011 1156 2573 Fragments arranged by size: 2573 2011 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5740 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2185 3471 4429 Size: 416 53 83 399 24 768 1286 958 Cuts at: 4429 442 Size: 1753 Fragments arranged by size: 1753 1286 958 768 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5740 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4400 1239 Size: 3161 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2537 3865 4730 5028 2537 Size: 1328 865 298 3249 Fragments arranged by size: 3249 1328 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4403 882 Size: 617 2904 2219 Fragments arranged by size: 2904 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3531 493 Size: 711 2327 2702 Fragments arranged by size: 2702 2327 711 BsaBI GATnn'nnATC Cuts at: 5734 5734 Size: 5740 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3547 3929 4564 275 Size: 53 83 186 936 2014 382 635 1451 Fragments arranged by size: 2014 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4173 4571 4845 4992 5403 4173 Size: 398 274 147 411 4510 Fragments arranged by size: 4510 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5515 Fragments arranged by size: 5515 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2237 1186 Size: 1051 4689 BseYI C'CCAG_C Cuts at: 2062 4943 5439 2062 Size: 2881 496 2363 Fragments arranged by size: 2881 2363 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5167 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2015 2149 3190 3951 4979 1540 Size: 275 186 14 134 1041 761 1028 2301 Fragments arranged by size: 2301 1041 1028 761 275 186 134 14 BsiHKAI G_wGCw'C Cuts at: 2515 3801 3886 4957 2515 Size: 1286 85 1071 3298 Fragments arranged by size: 3298 1286 1071 85 BsmI GAATG_Cn' Cuts at: 3251 3344 3251 Size: 93 5647 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2025 2515 3059 3801 3886 4957 1391 Size: 399 235 490 544 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 544 490 399 235 85 BspEI T'CCGG_A Cuts at: 5403 5403 Size: 5740 BspHI T'CATG_A Cuts at: 2938 3629 5359 2938 Size: 691 1730 3319 Fragments arranged by size: 3319 1730 691 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2905 3095 844 Size: 2061 190 3489 Fragments arranged by size: 3489 2061 190 BsrBI CCG'CTC Cuts at: 1394 1823 3627 1394 Size: 429 1804 3507 Fragments arranged by size: 3507 1804 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2310 2361 2571 4235 4409 65 Size: 1183 1062 51 210 1664 174 1396 Fragments arranged by size: 1664 1396 1183 1062 210 174 51 BsrFI r'CCGG_y Cuts at: 1700 1810 2014 4384 4571 1700 Size: 110 204 2370 187 2869 Fragments arranged by size: 2869 2370 204 187 110 BsrGI T'GTAC_A Cuts at: 96 2859 2871 96 Size: 2763 12 2965 Fragments arranged by size: 2965 2763 12 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5740 BssSI C'ACGA_G Cuts at: 3800 4812 3800 Size: 1012 4728 BstAPI GCAn_nnn'nTGC Cuts at: 794 2372 794 Size: 1578 4162 BstBI TT'CG_AA Cuts at: 4544 4544 Size: 5740 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5740 BstYI r'GATC_y Cuts at: 1972 2287 2910 3070 3437 3822 3839 5291 5377 Size: 315 623 160 367 385 17 1452 86 Cuts at: 5377 5389 5735 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 623 385 367 346 315 160 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2110 513 Size: 552 1045 4143 Fragments arranged by size: 4143 1045 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5001 Fragments arranged by size: 5001 683 56 BtsI GCAGTG_nn' Cuts at: 2661 2862 3256 4050 4070 2661 Size: 201 394 794 20 4331 Fragments arranged by size: 4331 794 394 201 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5740 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5705 DraI TTT'AAA Cuts at: 2538 3044 2538 Size: 506 5234 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4747 809 Size: 507 3431 1802 Fragments arranged by size: 3431 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2012 2077 3187 Size: 54 1425 50 275 18 182 65 1110 Cuts at: 3187 4076 5705 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1110 889 275 182 65 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2012 3187 1537 Size: 275 200 1175 4090 Fragments arranged by size: 4090 1175 275 200 EarI CTCTTCn'nnn_ Cuts at: 1353 2296 3670 1353 Size: 943 1374 3423 Fragments arranged by size: 3423 1374 943 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4327 4701 4847 387 Size: 1064 138 2738 374 146 1280 Fragments arranged by size: 2738 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2237 3803 4400 5187 5455 1239 Size: 393 605 1566 597 787 268 1524 Fragments arranged by size: 1566 1524 787 605 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2219 2219 Size: 5740 EcoO109I rG'GnC_Cy Cuts at: 2059 2059 Size: 5740 EcoRI G'AATT_C Cuts at: 2432 3049 3427 2432 Size: 617 378 4745 Fragments arranged by size: 4745 617 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2453 2453 Size: 5740 FspI TGC'GCA Cuts at: 4246 4246 Size: 5740 HaeII r_GCGC'y Cuts at: 1678 2032 4887 1678 Size: 354 2855 2531 Fragments arranged by size: 2855 2531 354 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2541 2573 4381 4413 4455 Size: 32 135 32 969 32 1808 32 42 Cuts at: 4455 4487 1373 Size: 32 2626 Fragments arranged by size: 2626 1808 969 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2743 3084 3330 669 Size: 988 281 805 341 246 3079 Fragments arranged by size: 3079 988 805 341 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5740 HpaI GTT'AAC Cuts at: 2743 3330 2743 Size: 587 5153 KpnI G_GTAC'C Cuts at: 3065 3065 Size: 5740 MfeI C'AATT_G Cuts at: 3339 3339 Size: 5740 MluI A'CGCG_T Cuts at: 5616 5616 Size: 5740 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2209 2216 4854 5038 1305 Size: 904 7 2638 184 2007 Fragments arranged by size: 2638 2007 904 184 7 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3918 Fragments arranged by size: 3918 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3698 4057 4216 518 Size: 648 2532 359 159 2042 Fragments arranged by size: 2532 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2066 2112 2849 2960 3156 3833 4981 5226 Size: 460 46 737 111 196 677 1148 245 Cuts at: 5226 1606 Size: 2120 Fragments arranged by size: 2120 1148 737 677 460 245 196 111 46 NaeI GCC'GGC Cuts at: 2016 2016 Size: 5740 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5188 NdeI CA'TA_TG Cuts at: 387 387 Size: 5740 NgoMIV G'CCGG_C Cuts at: 2014 2014 Size: 5740 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5740 NotI GC'GGCC_GC Cuts at: 3187 3187 Size: 5740 NspI r_CATG'y Cuts at: 1132 1229 3098 4643 1132 Size: 97 1869 1545 2229 Fragments arranged by size: 2229 1869 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5740 PciI A'CATG_T Cuts at: 4639 4639 Size: 5740 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5740 PfoI T'CCnGG_A Cuts at: 2749 2749 Size: 5740 PmeI GTTT'AAAC Cuts at: 3044 3044 Size: 5740 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3809 3841 5322 5354 3809 Size: 32 1481 32 4195 Fragments arranged by size: 4195 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2059 2059 Size: 5740 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5740 PsiI TTA'TAA Cuts at: 3310 3310 Size: 5740 PstI C_TGCA'G Cuts at: 830 3092 830 Size: 2262 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5740 PvuII CAG'CTG Cuts at: 2849 2960 2849 Size: 111 5629 SacII CC_GC'GG Cuts at: 2113 2113 Size: 5740 SalI G'TCGA_C Cuts at: 1936 3082 1936 Size: 1146 4594 SanDI GG'GwC_CC Cuts at: 2059 2059 Size: 5740 SbfI CC_TGCA'GG Cuts at: 3092 3092 Size: 5740 ScaI AGT'ACT Cuts at: 3988 5700 3988 Size: 1712 4028 SexAI A'CCwGG_T Cuts at: 2463 2463 Size: 5740 SfcI C'TryA_G Cuts at: 826 1045 3088 4223 4904 5095 826 Size: 219 2043 1135 681 191 1471 Fragments arranged by size: 2043 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5740 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5740 SmaI CCC'GGG Cuts at: 1872 3067 1872 Size: 1195 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2516 3844 4745 5007 820 Size: 197 926 573 1328 901 262 1553 Fragments arranged by size: 1553 1328 926 901 573 262 197 SnaBI TAC'GTA Cuts at: 493 3531 493 Size: 3038 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5740 SphI G_CATG'C Cuts at: 1132 3098 1132 Size: 1966 3774 SspI AAT'ATT Cuts at: 5 52 2140 2321 3664 5 Size: 47 2088 181 1343 2081 Fragments arranged by size: 2088 2081 1343 181 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5740 StyI C'CwwG_G Cuts at: 513 1065 2045 3179 513 Size: 552 980 1134 3074 Fragments arranged by size: 3074 1134 980 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2482 2777 3767 3784 3937 4122 4567 Size: 1255 902 295 990 17 153 185 445 Cuts at: 4567 325 Size: 1498 Fragments arranged by size: 1498 1255 990 902 445 295 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2859 2871 3986 Size: 274 80 33 51 1443 882 12 1115 Cuts at: 3986 5698 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1115 882 274 138 80 51 33 12 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5700 5732 5700 Size: 32 5708 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5740 XbaI T'CTAG_A Cuts at: 3076 3076 Size: 5740 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5740 XmaI C'CCGG_G Cuts at: 1870 3065 1870 Size: 1195 4545 XmnI GAAnn'nnTTC Cuts at: 2141 2436 3869 2141 Size: 295 1433 4012 Fragments arranged by size: 4012 1433 295 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3548 276 Size: 53 83 186 936 2014 2468 Fragments arranged by size: 2468 2014 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SapI SfiI SfoI SrfI SwaI XcmI