(Linear) (Six-base) MAPSORT of: KIEE3135.seq Check: 4024 from: 1 to: 1035 KIEE3135.orf With 182 enzymes: SgfI * December 27, 2011 16:33 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 232 1035 Size: 232 803 AlwNI CAG_nnn'CTG Cuts at: 0 892 1035 Size: 892 143 ApoI r'AATT_y Cuts at: 0 199 264 378 427 728 1035 Size: 199 65 114 49 301 307 Fragments arranged by size: 307 301 199 114 65 49 AvaI C'yCGr_G Cuts at: 0 187 1035 Size: 187 848 BanII G_rGCy'C Cuts at: 0 20 1035 Size: 20 1015 BglI GCCn_nnn'nGGC Cuts at: 0 15 1035 Size: 15 1020 BglII A'GATC_T Cuts at: 0 905 1035 Size: 905 130 BmrI ACTGGGnnnn_n' Cuts at: 0 180 1035 Size: 180 855 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 532 1035 Size: 532 503 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 232 1035 Size: 232 803 BseYI C'CCAG_C Cuts at: 0 57 1035 Size: 57 978 BsiEI CG_ry'CG Cuts at: 0 10 144 1035 Size: 10 134 891 Fragments arranged by size: 891 134 10 BsiHKAI G_wGCw'C Cuts at: 0 510 1035 Size: 510 525 Bsp1286I G_dGCh'C Cuts at: 0 20 510 1035 Size: 20 490 525 Fragments arranged by size: 525 490 20 BspHI T'CATG_A Cuts at: 0 933 1035 Size: 933 102 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 900 1035 Size: 900 135 BsrDI GCAATG_nn' Cuts at: 0 305 356 566 1035 Size: 305 51 210 469 Fragments arranged by size: 469 305 210 51 BsrFI r'CCGG_y Cuts at: 0 9 1035 Size: 9 1026 BsrGI T'GTAC_A Cuts at: 0 854 866 1035 Size: 854 12 169 Fragments arranged by size: 854 169 12 BstAPI GCAn_nnn'nTGC Cuts at: 0 367 1035 Size: 367 668 BstYI r'GATC_y Cuts at: 0 282 905 1035 Size: 282 623 130 Fragments arranged by size: 623 282 130 BtgI C'CryG_G Cuts at: 0 105 1035 Size: 105 930 BtsI GCAGTG_nn' Cuts at: 0 656 857 1035 Size: 656 201 178 Fragments arranged by size: 656 201 178 DraI TTT'AAA Cuts at: 0 533 1035 Size: 533 502 EaeI y'GGCC_r Cuts at: 0 7 72 1035 Size: 7 65 963 Fragments arranged by size: 963 65 7 EagI C'GGCC_G Cuts at: 0 7 1035 Size: 7 1028 EarI CTCTTCn'nnn_ Cuts at: 0 291 1035 Size: 291 744 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 232 1035 Size: 232 803 EcoNI CCTnn'n_nnAGG Cuts at: 0 214 1035 Size: 214 821 EcoO109I rG'GnC_Cy Cuts at: 0 54 1035 Size: 54 981 EcoRI G'AATT_C Cuts at: 0 427 1035 Size: 427 608 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 448 1035 Size: 448 587 HaeII r_GCGC'y Cuts at: 0 27 1035 Size: 27 1008 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 536 568 1035 Size: 536 32 467 Fragments arranged by size: 536 467 32 HincII GTy'rAC Cuts at: 0 738 1035 Size: 738 297 HpaI GTT'AAC Cuts at: 0 738 1035 Size: 738 297 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 204 211 1035 Size: 204 7 824 Fragments arranged by size: 824 204 7 MspA1I CmG'CkG Cuts at: 0 61 107 844 955 1035 Size: 61 46 737 111 80 Fragments arranged by size: 737 111 80 61 46 NaeI GCC'GGC Cuts at: 0 11 1035 Size: 11 1024 NgoMIV G'CCGG_C Cuts at: 0 9 1035 Size: 9 1026 PfoI T'CCnGG_A Cuts at: 0 744 1035 Size: 744 291 PpuMI rG'GwC_Cy Cuts at: 0 54 1035 Size: 54 981 PvuII CAG'CTG Cuts at: 0 844 955 1035 Size: 844 111 80 Fragments arranged by size: 844 111 80 SacII CC_GC'GG Cuts at: 0 108 1035 Size: 108 927 SanDI GG'GwC_CC Cuts at: 0 54 1035 Size: 54 981 SexAI A'CCwGG_T Cuts at: 0 458 1035 Size: 458 577 SmlI C'TyrA_G Cuts at: 0 511 1035 Size: 511 524 SspI AAT'ATT Cuts at: 0 135 316 1035 Size: 135 181 719 Fragments arranged by size: 719 181 135 StyI C'CwwG_G Cuts at: 0 40 1035 Size: 40 995 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 477 772 1035 Size: 477 295 263 Fragments arranged by size: 477 295 263 TatI w'GTAC_w Cuts at: 0 854 866 1035 Size: 854 12 169 Fragments arranged by size: 854 169 12 XmnI GAAnn'nnTTC Cuts at: 0 136 431 1035 Size: 136 295 604 Fragments arranged by size: 604 295 136 Enzymes that do cut: AcuI AlwNI ApoI AvaI BanII BglI BglII BmrI BpuEI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BstAPI BstYI BtgI BtsI DraI EaeI EagI EarI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII HpaI MmeI MspA1I NaeI NgoMIV PfoI PpuMI PvuII SacII SanDI SexAI SmlI SspI StyI TaqII TatI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BlpI Bme1580I BmgBI BmtI BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BsgI BsiWI BsmI BsmBI BspEI BsrBI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraIII DrdI EciI EcoICRI EcoRV FseI FspI FspAI HindIII KasI KpnI MfeI MluI MscI MslI NarI NcoI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI TstI Tth111I XbaI XcmI XhoI XmaI ZraI