################################## # NO CUT ENZYMES ( 167 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BseAI BsgI BsiEI BsiWI BsmAI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI DsaI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MaeII MamI MfeI MflI MluI MroI MseI MslI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp509I Tth111I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AfaI 228 GTAC GT^AC AhdI 461 GACTTCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 461 GACTTCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 165 CCCGGG C^[CT]CG[GA]G BalI 29 TGGCCA TGG^CCA BanII 412 GAGCTC G[GA]GC[CT]^C BsaWI 107 ACCGGA [AT]^CCGG[AT] BseRI 490 CTCCTC GAGGAG(10/8) BsoBI 165 CCCGGG C^[CT]CG[GA]G BspMI 387 GCAGGT ACCTGC(4/8) BsrDI 430 CATTGC GCAATG(2/0) BsrFI 316 ACCGGC [GA]^CCGG[CT] BstEII 468 GGTCACC G^GT[ACGT]ACC BstF5I 264 CATCC GGATG(2/0) BstPI 468 GGTCACC G^GT[ACGT]ACC CfoI 455 GCGC GCG^C Cfr10I 316 ACCGGC [GA]^CCGG[CT] DraII 113 AGGCCCT [GA]G^G[ACGT]CC[CT] EaeI 29 TGGCCA [CT]^GGCC[GA] Eam1105I 461 GACTTCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 49 CTCTTC CTCTTC(1/4) EcoO109I 113 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoO65I 468 GGTCACC G^GT[ACGT]ACC EcoT14I 299 CCAAGG C^C[AT][AT]GG FokI 264 CATCC GGATG(9/13) HhaI 455 GCGC GCG^C HinP1I 455 GCGC G^CGC HinfI 95 GAATC G^A[ACGT]TC Ksp632I 49 CTCTTC CTCTTC(1/4) MboII 50 TCTTC GAAGA(8/7) MluNI 29 TGGCCA TGG^CCA MscI 29 TGGCCA TGG^CCA NspI 41 GCATGC [GA]CATG^[CT] PflMI 239 CCAACTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RsaI 228 GTAC GT^AC SacI 412 GAGCTC GAGCT^C SapI 48 GCTCTTC GCTCTTC(1/4) SmaI 165 CCCGGG CCC^GGG SmlI 424 CTCAAG C^T[CT][GA]AG SphI 41 GCATGC GCATG^C StyI 299 CCAAGG C^C[AT][AT]GG TaqI 197 TCGA T^CGA TfiI 95 GAATC G^A[AT]TC TthHB8I 197 TCGA T^CGA Van91I 239 CCAACTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 165 CCCGGG C^CCGGG XmaI 165 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 9 enzymes) # ################################### AvaII 69 GGTCC G^G[AT]CC 405 GGTCC BcnI 165 CCCGG CC^[GC]GG 166 CCGGG BglI 145 GCCCAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 331 GCCACCCAGGC BsrI 245 ACTGG ACTGG(1/-1) 484 CCAGT DpnII 135 GATC ^GATC 510 GATC MboI 135 GATC ^GATC 510 GATC NciI 165 CCCGG CC^[GC]GG 166 CCGGG NdeII 135 GATC ^GATC 510 GATC Sau3AI 135 GATC ^GATC 510 GATC ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AciI AluI AspHI BbvI BmyI BsaJI BsiHKAI BsiYI BslI Bsp1286I BssKI BstNI Cac8I Cfr13I DdeI EcoRII Fnu4HI HaeIII HapII HpaII HphI ItaI MaeIII MnlI MspI MvaI MwoI NlaIII NlaIV Sau96I ScrFI SfaNI TseI Tsp45I TspRI