(Circular) (Six-base) MAPSORT of: pFN21AE6509.seq Check: 2545 from: 1 to: 5233 pFN21AE6509 5233 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2383 2383 Size: 5233 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3043 278 Size: 53 83 186 936 1507 2468 Fragments arranged by size: 2468 1507 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2576 1937 Size: 639 4594 Acc65I G'GTAC_C Cuts at: 2554 2554 Size: 5233 AclI AA'CG_TT Cuts at: 1563 3360 3733 1563 Size: 1797 373 3063 Fragments arranged by size: 3063 1797 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2041 3296 4680 4948 1632 Size: 409 1255 1384 268 1917 Fragments arranged by size: 1917 1384 1255 409 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5036 AflIII A'CryG_T Cuts at: 1203 4132 5109 1203 Size: 2929 977 1327 Fragments arranged by size: 2929 1327 977 AgeI A'CCGG_T Cuts at: 4064 4064 Size: 5233 AhdI GACnn_n'nnGTC Cuts at: 2471 3962 2471 Size: 1491 3742 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5233 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5233 AlwNI CAG_nnn'CTG Cuts at: 2976 4548 2976 Size: 1572 3661 ApaLI G'TGCA_C Cuts at: 3290 4446 3290 Size: 1156 4077 ApoI r'AATT_y Cuts at: 1493 2542 2768 2920 5029 1493 Size: 1049 226 152 2109 1697 Fragments arranged by size: 2109 1697 1049 226 152 AseI AT'TA_AT Cuts at: 160 1032 3787 160 Size: 872 2755 1606 Fragments arranged by size: 2755 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5233 AvaI C'yCGr_G Cuts at: 1870 1943 2170 2558 1870 Size: 73 227 388 4545 Fragments arranged by size: 4545 388 227 73 BamHI G'GATC_C Cuts at: 2563 2930 2563 Size: 367 4866 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2554 4009 618 Size: 325 217 627 767 1455 1842 Fragments arranged by size: 1842 1455 767 627 325 217 BanII G_rGCy'C Cuts at: 1391 2421 2552 1391 Size: 1030 131 4072 Fragments arranged by size: 4072 1030 131 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4262 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3424 3458 1903 Size: 34 1487 34 3678 Fragments arranged by size: 3678 1487 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3125 4335 3125 Size: 1210 4023 BclI T'GATC_A Cuts at: 1548 1548 Size: 5233 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2156 2342 3844 Size: 107 122 71 631 605 484 186 1502 Cuts at: 3844 136 Size: 1525 Fragments arranged by size: 1525 1502 631 605 484 186 122 107 71 BglII A'GATC_T Cuts at: 5228 5228 Size: 5233 BlpI GC'TnA_GC Cuts at: 2650 2650 Size: 5233 Bme1580I G_kGCm'C Cuts at: 1790 3294 4450 1790 Size: 1504 1156 2573 Fragments arranged by size: 2573 1504 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5233 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2964 3922 442 Size: 416 53 83 399 24 1547 958 1753 Fragments arranged by size: 1753 1547 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5233 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2440 2440 Size: 5233 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3893 1239 Size: 2654 2579 Bpu10I CC'TnA_GC Cuts at: 2018 2018 Size: 5233 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2414 3358 4223 4521 2414 Size: 944 865 298 3126 Fragments arranged by size: 3126 944 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3896 882 Size: 617 2397 2219 Fragments arranged by size: 2397 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3024 493 Size: 711 1820 2702 Fragments arranged by size: 2702 1820 711 BsaBI GATnn'nnATC Cuts at: 5227 5227 Size: 5233 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3040 3422 4057 275 Size: 53 83 186 936 1507 382 635 1451 Fragments arranged by size: 1507 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2112 3666 4064 4338 4485 4896 2112 Size: 1554 398 274 147 411 2449 Fragments arranged by size: 2449 1554 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5008 Fragments arranged by size: 5008 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2486 1186 Size: 1300 3933 BseYI C'CCAG_C Cuts at: 2126 4436 4932 2126 Size: 2310 496 2427 Fragments arranged by size: 2427 2310 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4660 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2683 3444 4472 1540 Size: 275 186 682 761 1028 2301 Fragments arranged by size: 2301 1028 761 682 275 186 BsiHKAI G_wGCw'C Cuts at: 2400 2421 2496 3294 3379 4450 2400 Size: 21 75 798 85 1071 3183 Fragments arranged by size: 3183 1071 798 85 75 21 BsmI GAATG_Cn' Cuts at: 2744 2837 2744 Size: 93 5140 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2400 2421 2496 2552 3294 3379 4450 Size: 399 610 21 75 56 742 85 1071 Cuts at: 4450 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 610 399 85 75 56 21 BspEI T'CCGG_A Cuts at: 4896 4896 Size: 5233 BspHI T'CATG_A Cuts at: 3122 4852 3122 Size: 1730 3503 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2383 2588 844 Size: 1539 205 3489 Fragments arranged by size: 3489 1539 205 BsrBI CCG'CTC Cuts at: 1394 1823 3120 1394 Size: 429 1297 3507 Fragments arranged by size: 3507 1297 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2434 3728 3902 65 Size: 1183 1186 1294 174 1396 Fragments arranged by size: 1396 1294 1186 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2321 3877 4064 1700 Size: 110 511 1556 187 2869 Fragments arranged by size: 2869 1556 511 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5233 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5233 BssSI C'ACGA_G Cuts at: 3293 4305 3293 Size: 1012 4221 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5233 BstBI TT'CG_AA Cuts at: 4037 4037 Size: 5233 BstEII G'GTnAC_C Cuts at: 1184 2473 1184 Size: 1289 3944 BstYI r'GATC_y Cuts at: 1972 2563 2930 3315 3332 4784 4870 4882 5228 Size: 591 367 385 17 1452 86 12 346 Cuts at: 5228 1972 Size: 1977 Fragments arranged by size: 1977 1452 591 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4681 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4494 Fragments arranged by size: 4494 683 56 BtsI GCAGTG_nn' Cuts at: 2130 2749 3543 3563 2130 Size: 619 794 20 3800 Fragments arranged by size: 3800 794 619 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5233 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5198 DraI TTT'AAA Cuts at: 2537 2537 Size: 5233 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4240 809 Size: 507 2924 1802 Fragments arranged by size: 2924 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2034 2680 3569 Size: 54 1425 50 275 18 204 646 889 Cuts at: 3569 5198 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 646 275 204 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2680 1537 Size: 275 868 4090 Fragments arranged by size: 4090 868 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2060 3163 1353 Size: 707 1103 3423 Fragments arranged by size: 3423 1103 707 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3820 4194 4340 387 Size: 1064 138 2231 374 146 1280 Fragments arranged by size: 2231 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2419 2419 Size: 5233 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2041 3296 3893 4680 4948 1239 Size: 393 409 1255 597 787 268 1524 Fragments arranged by size: 1524 1255 787 597 409 393 268 EcoO109I rG'GnC_Cy Cuts at: 2119 2119 Size: 5233 EcoRI G'AATT_C Cuts at: 2542 2920 2542 Size: 378 4855 FspI TGC'GCA Cuts at: 3739 3739 Size: 5233 HaeII r_GCGC'y Cuts at: 1678 4380 1678 Size: 2702 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2166 2198 2457 2489 3874 Size: 32 135 32 594 32 259 32 1385 Cuts at: 3874 3906 3948 3980 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1385 594 259 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2577 2823 669 Size: 988 281 639 246 3079 Fragments arranged by size: 3079 988 639 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5233 HpaI GTT'AAC Cuts at: 2823 2823 Size: 5233 KpnI G_GTAC'C Cuts at: 2558 2558 Size: 5233 MfeI C'AATT_G Cuts at: 2832 2832 Size: 5233 MluI A'CGCG_T Cuts at: 5109 5109 Size: 5233 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2430 4347 4531 1305 Size: 1125 1917 184 2007 Fragments arranged by size: 2007 1917 1125 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2036 10 Size: 54 1425 343 204 3207 Fragments arranged by size: 3207 1425 343 204 54 MslI CAynn'nnrTG Cuts at: 518 1166 3191 3550 3709 518 Size: 648 2025 359 159 2042 Fragments arranged by size: 2042 2025 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2649 3326 4474 4719 1606 Size: 1043 677 1148 245 2120 Fragments arranged by size: 2120 1148 1043 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4681 NdeI CA'TA_TG Cuts at: 387 387 Size: 5233 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5233 NotI GC'GGCC_GC Cuts at: 2680 2680 Size: 5233 NspI r_CATG'y Cuts at: 1132 1229 2050 2591 4136 1132 Size: 97 821 541 1545 2229 Fragments arranged by size: 2229 1545 821 541 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5233 PciI A'CATG_T Cuts at: 4132 4132 Size: 5233 PflMI CCAn_nnn'nTGG Cuts at: 1108 2250 1108 Size: 1142 4091 PfoI T'CCnGG_A Cuts at: 2412 2412 Size: 5233 PmeI GTTT'AAAC Cuts at: 2537 2537 Size: 5233 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3302 3334 4815 4847 3302 Size: 32 1481 32 3688 Fragments arranged by size: 3688 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5233 PsiI TTA'TAA Cuts at: 2803 2803 Size: 5233 PstI C_TGCA'G Cuts at: 830 2585 830 Size: 1755 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5233 SacI G_AGCT'C Cuts at: 2421 2421 Size: 5233 SalI G'TCGA_C Cuts at: 1936 2575 1936 Size: 639 4594 SapI GCTCTTCn'nnn_ Cuts at: 2060 2060 Size: 5233 SbfI CC_TGCA'GG Cuts at: 2585 2585 Size: 5233 ScaI AGT'ACT Cuts at: 3481 5193 3481 Size: 1712 3521 SfcI C'TryA_G Cuts at: 826 1045 2581 3716 4397 4588 826 Size: 219 1536 1135 681 191 1471 Fragments arranged by size: 1536 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5233 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5233 SmaI CCC'GGG Cuts at: 1872 2172 2560 1872 Size: 300 388 4545 Fragments arranged by size: 4545 388 300 SmlI C'TyrA_G Cuts at: 820 1017 1943 2429 3337 4238 4500 820 Size: 197 926 486 908 901 262 1553 Fragments arranged by size: 1553 926 908 901 486 262 197 SnaBI TAC'GTA Cuts at: 493 3024 493 Size: 2531 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5233 SphI G_CATG'C Cuts at: 1132 2050 2591 1132 Size: 918 541 3774 Fragments arranged by size: 3774 918 541 SspI AAT'ATT Cuts at: 5 52 3157 5 Size: 47 3105 2081 Fragments arranged by size: 3105 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5233 StyI C'CwwG_G Cuts at: 513 1065 2304 2672 513 Size: 552 1239 368 3074 Fragments arranged by size: 3074 1239 552 368 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2354 3260 3277 3430 3615 4060 325 Size: 1255 774 906 17 153 185 445 1498 Fragments arranged by size: 1498 1255 906 774 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3479 5191 96 Size: 274 80 33 51 1443 1502 1712 138 Fragments arranged by size: 1712 1502 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5193 5225 5193 Size: 32 5201 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5233 XbaI T'CTAG_A Cuts at: 2569 2569 Size: 5233 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5233 XmaI C'CCGG_G Cuts at: 1870 2170 2558 1870 Size: 300 388 4545 Fragments arranged by size: 4545 388 300 XmnI GAAnn'nnTTC Cuts at: 3362 3362 Size: 5233 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3041 276 Size: 53 83 186 936 1507 2468 Fragments arranged by size: 2468 1507 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI