(Linear) (Six-base) MAPSORT of: KIEE6509.seq Check: 4330 from: 1 to: 528 KIEE6509.orf With 182 enzymes: SgfI * March 16, 2015 11:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 378 528 Size: 378 150 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 36 528 Size: 36 492 AhdI GACnn_n'nnGTC Cuts at: 0 466 528 Size: 466 62 AvaI C'yCGr_G Cuts at: 0 165 528 Size: 165 363 BanII G_rGCy'C Cuts at: 0 416 528 Size: 416 112 BglI GCCn_nnn'nGGC Cuts at: 0 151 337 528 Size: 151 186 191 Fragments arranged by size: 191 186 151 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 435 528 Size: 435 93 Bpu10I CC'TnA_GC Cuts at: 0 13 528 Size: 13 515 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 409 528 Size: 409 119 BsaWI w'CCGG_w Cuts at: 0 107 528 Size: 107 421 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 481 528 Size: 481 47 BseYI C'CCAG_C Cuts at: 0 121 528 Size: 121 407 BsiHKAI G_wGCw'C Cuts at: 0 395 416 491 528 Size: 395 21 75 37 Fragments arranged by size: 395 75 37 21 Bsp1286I G_dGCh'C Cuts at: 0 395 416 491 528 Size: 395 21 75 37 Fragments arranged by size: 395 75 37 21 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 378 528 Size: 378 150 BsrDI GCAATG_nn' Cuts at: 0 429 528 Size: 429 99 BsrFI r'CCGG_y Cuts at: 0 316 528 Size: 316 212 BstEII G'GTnAC_C Cuts at: 0 468 528 Size: 468 60 BtsI GCAGTG_nn' Cuts at: 0 125 528 Size: 125 403 EaeI y'GGCC_r Cuts at: 0 29 528 Size: 29 499 EarI CTCTTCn'nnn_ Cuts at: 0 55 528 Size: 55 473 EcoICRI GAG'CTC Cuts at: 0 414 528 Size: 414 114 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 36 528 Size: 36 492 EcoO109I rG'GnC_Cy Cuts at: 0 114 528 Size: 114 414 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 161 193 452 484 528 Size: 161 32 259 32 44 Fragments arranged by size: 259 161 44 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 425 528 Size: 425 103 MscI TGG'CCA Cuts at: 0 31 528 Size: 31 497 NspI r_CATG'y Cuts at: 0 45 528 Size: 45 483 PflMI CCAn_nnn'nTGG Cuts at: 0 245 528 Size: 245 283 PfoI T'CCnGG_A Cuts at: 0 407 528 Size: 407 121 SacI G_AGCT'C Cuts at: 0 416 528 Size: 416 112 SapI GCTCTTCn'nnn_ Cuts at: 0 55 528 Size: 55 473 SmaI CCC'GGG Cuts at: 0 167 528 Size: 167 361 SmlI C'TyrA_G Cuts at: 0 424 528 Size: 424 104 SphI G_CATG'C Cuts at: 0 45 528 Size: 45 483 StyI C'CwwG_G Cuts at: 0 299 528 Size: 299 229 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 349 528 Size: 349 179 XmaI C'CCGG_G Cuts at: 0 165 528 Size: 165 363 Enzymes that do cut: AarI AcuI AhdI AvaI BanII BglI BplI Bpu10I BpuEI BsaWI BseRI BseYI BsiHKAI Bsp1286I BspMI BsrDI BsrFI BstEII BtsI EaeI EarI EcoICRI Eco57MI EcoO109I Hin4I MmeI MscI NspI PflMI PfoI SacI SapI SmaI SmlI SphI StyI TaqII XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BsaI BsaAI BsaBI BsaHI BsaXI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI