(Circular) (Six-base) MAPSORT of: pFN21AE6626.seq Check: 4552 from: 1 to: 5647 pFN21AE6626 5647 bp With 182 enzymes: SgfI * June 20, 2019 11:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3457 278 Size: 53 83 186 936 1921 2468 Fragments arranged by size: 2468 1921 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2990 1937 Size: 1053 4594 Acc65I G'GTAC_C Cuts at: 2968 2968 Size: 5647 AclI AA'CG_TT Cuts at: 1563 3774 4147 1563 Size: 2211 373 3063 Fragments arranged by size: 3063 2211 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2600 3710 5094 5362 1632 Size: 968 1110 1384 268 1917 Fragments arranged by size: 1917 1384 1110 968 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5450 AflIII A'CryG_T Cuts at: 1203 4546 5523 1203 Size: 3343 977 1327 Fragments arranged by size: 3343 1327 977 AgeI A'CCGG_T Cuts at: 4478 4478 Size: 5647 AhdI GACnn_n'nnGTC Cuts at: 4376 4376 Size: 5647 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5647 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5647 AlwNI CAG_nnn'CTG Cuts at: 2758 3390 4962 2758 Size: 632 1572 3443 Fragments arranged by size: 3443 1572 632 ApaI G_GGCC'C Cuts at: 2160 2160 Size: 5647 ApaLI G'TGCA_C Cuts at: 3704 4860 3704 Size: 1156 4491 ApoI r'AATT_y Cuts at: 1493 2956 3182 3334 5443 1493 Size: 1463 226 152 2109 1697 Fragments arranged by size: 2109 1697 1463 226 152 AseI AT'TA_AT Cuts at: 160 1032 4201 160 Size: 872 3169 1606 Fragments arranged by size: 3169 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5647 AvaI C'yCGr_G Cuts at: 1870 1943 2447 2972 1870 Size: 73 504 525 4545 Fragments arranged by size: 4545 525 504 73 BamHI G'GATC_C Cuts at: 2977 3344 2977 Size: 367 5280 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2011 2199 2968 4423 618 Size: 325 217 627 224 188 769 1455 1842 Fragments arranged by size: 1842 1455 769 627 325 224 217 188 BanII G_rGCy'C Cuts at: 1391 2160 2245 2966 1391 Size: 769 85 721 4072 Fragments arranged by size: 4072 769 721 85 BbeI G_GCGC'C Cuts at: 2015 2203 2015 Size: 188 5459 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2706 928 Size: 971 807 3869 Fragments arranged by size: 3869 971 807 BbvCI CC'TCA_GC Cuts at: 2766 2766 Size: 5647 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3838 3872 1903 Size: 34 1901 34 3678 Fragments arranged by size: 3678 1901 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3539 4749 3539 Size: 1210 4437 BclI T'GATC_A Cuts at: 1548 1548 Size: 5647 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2840 4258 Size: 107 122 71 631 605 337 831 1418 Cuts at: 4258 136 Size: 1525 Fragments arranged by size: 1525 1418 831 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5642 5642 Size: 5647 BlpI GC'TnA_GC Cuts at: 3064 3064 Size: 5647 Bme1580I G_kGCm'C Cuts at: 1790 2160 3708 4864 1790 Size: 370 1548 1156 2573 Fragments arranged by size: 2573 1548 1156 370 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5647 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3378 4336 442 Size: 416 53 83 399 24 1961 958 1753 Fragments arranged by size: 1961 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5647 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2183 2205 2399 2519 4307 1239 Size: 944 22 194 120 1788 2579 Fragments arranged by size: 2579 1788 944 194 120 22 Bpu10I CC'TnA_GC Cuts at: 2766 2766 Size: 5647 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3772 4637 4935 3772 Size: 865 298 4484 Fragments arranged by size: 4484 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2612 4310 882 Size: 617 1113 1698 2219 Fragments arranged by size: 2219 1698 1113 617 BsaAI yAC'GTr Cuts at: 493 1204 3438 493 Size: 711 2234 2702 Fragments arranged by size: 2702 2234 711 BsaBI GATnn'nnATC Cuts at: 5641 5641 Size: 5647 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2012 2200 3454 3836 Size: 53 83 186 936 479 188 1254 382 Cuts at: 3836 4471 275 Size: 635 1451 Fragments arranged by size: 1451 1254 936 635 479 382 188 186 83 53 BsaWI w'CCGG_w Cuts at: 4080 4478 4752 4899 5310 4080 Size: 398 274 147 411 4417 Fragments arranged by size: 4417 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5422 Fragments arranged by size: 5422 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2135 2186 2294 2321 2396 2432 2447 2495 Size: 949 51 108 27 75 36 15 48 Cuts at: 2495 2498 2516 2519 2531 2534 2537 2555 2579 Size: 3 18 3 12 3 3 18 24 Cuts at: 2579 2615 1186 Size: 36 4218 Fragments arranged by size: 4218 949 108 75 51 48 36 36 27 24 18 18 15 12 3 3 3 3 BseYI C'CCAG_C Cuts at: 2148 2232 2244 2403 2454 2550 2941 4850 5346 Size: 84 12 159 51 96 391 1909 496 Cuts at: 5346 2148 Size: 2449 Fragments arranged by size: 2449 1909 496 391 159 96 84 51 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2096 1163 Size: 573 360 4714 Fragments arranged by size: 4714 573 360 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2797 3097 3858 4886 1540 Size: 275 186 796 300 761 1028 2301 Fragments arranged by size: 2301 1028 796 761 300 275 186 BsiHKAI G_wGCw'C Cuts at: 2694 3708 3793 4864 2694 Size: 1014 85 1071 3477 Fragments arranged by size: 3477 1071 1014 85 BsmI GAATG_Cn' Cuts at: 3158 3251 3158 Size: 93 5554 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2160 2245 2694 2966 3708 3793 4864 Size: 399 370 85 449 272 742 85 1071 Cuts at: 4864 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 449 399 370 272 85 85 BspEI T'CCGG_A Cuts at: 5310 5310 Size: 5647 BspHI T'CATG_A Cuts at: 3536 5266 3536 Size: 1730 3917 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2766 3002 844 Size: 1922 236 3489 Fragments arranged by size: 3489 1922 236 BsrBI CCG'CTC Cuts at: 1394 1823 2437 2488 3534 1394 Size: 429 614 51 1046 3507 Fragments arranged by size: 3507 1046 614 429 51 BsrDI GCAATG_nn' Cuts at: 65 1248 4142 4316 65 Size: 1183 2894 174 1396 Fragments arranged by size: 2894 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2096 4291 4478 1700 Size: 110 286 2195 187 2869 Fragments arranged by size: 2869 2195 286 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5647 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5647 BssSI C'ACGA_G Cuts at: 3707 4719 3707 Size: 1012 4635 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5647 BstBI TT'CG_AA Cuts at: 4451 4451 Size: 5647 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5647 BstYI r'GATC_y Cuts at: 1972 2977 3344 3729 3746 5198 5284 5296 5642 Size: 1005 367 385 17 1452 86 12 346 Cuts at: 5642 1972 Size: 1977 Fragments arranged by size: 1977 1452 1005 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2169 2169 Size: 5647 BtgI C'CryG_G Cuts at: 513 1065 2004 2838 513 Size: 552 939 834 3322 Fragments arranged by size: 3322 939 834 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2860 487 Size: 683 56 1634 3274 Fragments arranged by size: 3274 1634 683 56 BtsI GCAGTG_nn' Cuts at: 2108 3163 3957 3977 2108 Size: 1055 794 20 3778 Fragments arranged by size: 3778 1055 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5647 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5612 DraI TTT'AAA Cuts at: 2951 2951 Size: 5647 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4654 809 Size: 507 3338 1802 Fragments arranged by size: 3338 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2098 2832 2841 Size: 54 1425 50 275 18 268 734 9 Cuts at: 2841 3094 3983 5612 8 Size: 253 889 1629 43 Fragments arranged by size: 1629 1425 889 734 275 268 253 54 50 43 18 9 EagI C'GGCC_G Cuts at: 1537 1812 3094 1537 Size: 275 1282 4090 Fragments arranged by size: 4090 1282 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2066 3577 1353 Size: 713 1511 3423 Fragments arranged by size: 3423 1511 713 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4234 4608 4754 387 Size: 1064 138 2645 374 146 1280 Fragments arranged by size: 2645 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2183 2205 2399 2519 2600 3710 4307 Size: 393 551 22 194 120 81 1110 597 Cuts at: 4307 5094 5362 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1110 787 597 551 393 268 194 120 81 22 EcoNI CCTnn'n_nnAGG Cuts at: 2300 2501 2300 Size: 201 5446 EcoO109I rG'GnC_Cy Cuts at: 2157 2209 2755 2157 Size: 52 546 5049 Fragments arranged by size: 5049 546 52 EcoRI G'AATT_C Cuts at: 2956 3334 2956 Size: 378 5269 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2605 2605 Size: 5647 FspI TGC'GCA Cuts at: 4153 4153 Size: 5647 HaeII r_GCGC'y Cuts at: 1678 2015 2203 4794 1678 Size: 337 188 2591 2531 Fragments arranged by size: 2591 2531 337 188 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2621 2653 4288 4320 4362 Size: 32 135 32 1049 32 1635 32 42 Cuts at: 4362 4394 1373 Size: 32 2626 Fragments arranged by size: 2626 1635 1049 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2991 3237 669 Size: 988 281 1053 246 3079 Fragments arranged by size: 3079 1053 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5647 HpaI GTT'AAC Cuts at: 3237 3237 Size: 5647 KasI G'GCGC_C Cuts at: 2011 2199 2011 Size: 188 5459 KpnI G_GTAC'C Cuts at: 2972 2972 Size: 5647 MfeI C'AATT_G Cuts at: 3246 3246 Size: 5647 MluI A'CGCG_T Cuts at: 5523 5523 Size: 5647 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2878 4761 4945 1305 Size: 1573 1883 184 2007 Fragments arranged by size: 2007 1883 1573 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2843 10 Size: 54 1425 343 1011 2814 Fragments arranged by size: 2814 1425 1011 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3605 3964 4123 518 Size: 648 2439 359 159 2042 Fragments arranged by size: 2439 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2248 2330 2554 2860 3063 3740 4888 5133 Size: 642 82 224 306 203 677 1148 245 Cuts at: 5133 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 642 306 245 224 203 82 NaeI GCC'GGC Cuts at: 2098 2098 Size: 5647 NarI GG'CG_CC Cuts at: 2012 2200 2012 Size: 188 5459 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4156 Fragments arranged by size: 4156 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5647 NgoMIV G'CCGG_C Cuts at: 2096 2096 Size: 5647 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5647 NotI GC'GGCC_GC Cuts at: 3094 3094 Size: 5647 NspI r_CATG'y Cuts at: 1132 1229 3005 4550 1132 Size: 97 1776 1545 2229 Fragments arranged by size: 2229 1776 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5647 PciI A'CATG_T Cuts at: 4546 4546 Size: 5647 PflMI CCAn_nnn'nTGG Cuts at: 1108 2251 1108 Size: 1143 4504 PfoI T'CCnGG_A Cuts at: 2751 2751 Size: 5647 PmeI GTTT'AAAC Cuts at: 2951 2951 Size: 5647 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3716 3748 5229 5261 3716 Size: 32 1481 32 4102 Fragments arranged by size: 4102 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2209 2755 2209 Size: 546 5101 PshAI GACnn'nnGTC Cuts at: 1613 2449 1613 Size: 836 4811 PsiI TTA'TAA Cuts at: 3217 3217 Size: 5647 PspOMI G'GGCC_C Cuts at: 2156 2156 Size: 5647 PspXI vC'TCGA_Gb Cuts at: 2447 2447 Size: 5647 PstI C_TGCA'G Cuts at: 830 2999 830 Size: 2169 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5647 SalI G'TCGA_C Cuts at: 1936 2989 1936 Size: 1053 4594 SbfI CC_TGCA'GG Cuts at: 2999 2999 Size: 5647 ScaI AGT'ACT Cuts at: 3895 5607 3895 Size: 1712 3935 SfcI C'TryA_G Cuts at: 826 1045 2225 2995 4130 4811 5002 826 Size: 219 1180 770 1135 681 191 1471 Fragments arranged by size: 1471 1180 1135 770 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2840 2840 Size: 5647 SfoI GGC'GCC Cuts at: 2013 2201 2013 Size: 188 5459 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5647 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5647 SmaI CCC'GGG Cuts at: 1872 2974 1872 Size: 1102 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2447 3751 4652 4914 820 Size: 197 926 504 1304 901 262 1553 Fragments arranged by size: 1553 1304 926 901 504 262 197 SnaBI TAC'GTA Cuts at: 493 3438 493 Size: 2945 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5647 SphI G_CATG'C Cuts at: 1132 3005 1132 Size: 1873 3774 SspI AAT'ATT Cuts at: 5 52 3571 5 Size: 47 3519 2081 Fragments arranged by size: 3519 2081 47 StuI AGG'CCT Cuts at: 1517 2194 2588 1517 Size: 677 394 4576 Fragments arranged by size: 4576 677 394 StyI C'CwwG_G Cuts at: 513 1065 2004 2682 3086 513 Size: 552 939 678 404 3074 Fragments arranged by size: 3074 939 678 552 404 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3674 3691 3844 4029 4474 325 Size: 1255 2094 17 153 185 445 1498 Fragments arranged by size: 2094 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3893 5605 96 Size: 274 80 33 51 1443 1916 1712 138 Fragments arranged by size: 1916 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5607 5639 5607 Size: 32 5615 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5647 XbaI T'CTAG_A Cuts at: 2983 2983 Size: 5647 XhoI C'TCGA_G Cuts at: 1943 2447 1943 Size: 504 5143 XmaI C'CCGG_G Cuts at: 1870 2972 1870 Size: 1102 4545 XmnI GAAnn'nnTTC Cuts at: 3776 3776 Size: 5647 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3455 276 Size: 53 83 186 936 1921 2468 Fragments arranged by size: 2468 1921 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FseI FspAI NruI NsiI PacI PmlI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SrfI SwaI XcmI