################################## # NO CUT ENZYMES ( 113 enzymes) # ################################## AatII Acc65I AclI AcyI AflII AflIII AgeI AhdI AlwNI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BbeI BfrI BglI BglII BlnI BlpI Bpu1102I BsaHI BsiWI BsmI BsoBI Bsp1407I BspLU11I BsrBI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI Hin1I HincII HindII HpaI KasI KpnI KspI MfeI MluI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp45I Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccI 1608 GTCTAC GT^[AC][GT]AC AccIII 591 TCCGGA T^CCGGA Alw44I 914 GTGCAC G^TGCAC ApaLI 914 GTGCAC G^TGCAC Asp700I 1752 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 953 TGGCCA TGG^CCA BamHI 288 GGATCC G^GATCC BanII 1010 GAGCCC G[GA]GC[CT]^C BbrPI 13 CACGTG CAC^GTG BbvCI 355 GCTGAGG CCTCAGC(-5/-2) BcgI 1141 CGAATCGATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 960 GTATCC GTATCC(6/5) BclI 500 TGATCA T^GATCA BcnI 486 CCGGG CC^[GC]GG BfaI 1307 CTAG C^TAG BsaAI 13 CACGTG [CT]AC^GT[GA] BsaBI 783 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 591 TCCGGA [AT]^CCGG[AT] BseAI 591 TCCGGA T^CCGGA BsiEI 32 CGGTCG CG[GA][CT]^CG BspDI 1144 ATCGAT AT^CGAT BspEI 591 TCCGGA T^CCGGA BsrDI 1270 GCAATG GCAATG(2/0) BsrFI 1698 GCCGGT [GA]^CCGG[CT] BssSI 1167 CACGAG CACGAG(-5/-1) Cfr10I 1698 GCCGGT [GA]^CCGG[CT] ClaI 1144 ATCGAT AT^CGAT DrdI 408 GACCGATCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 752 CCATGG C^C[GA][CT]GG FbaI 500 TGATCA T^GATCA HaeII 51 GGCGCT [GA]GCGC^[CT] HgaI 1378 GCGTC GACGC(5/10) HindIII 1186 AAGCTT A^AGCTT MaeI 1307 CTAG C^TAG MaeIII 1028 GTAAC ^GT[ACGT]AC MamI 783 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 953 TGGCCA TGG^CCA MroI 591 TCCGGA T^CCGGA MscI 953 TGGCCA TGG^CCA NciI 486 CCGGG CC^[GC]GG NcoI 752 CCATGG C^CATGG NspI 930 ACATGC [GA]CATG^[CT] PmaCI 13 CACGTG CAC^GTG PmlI 13 CACGTG CAC^GTG PstI 82 CTGCAG CTGCA^G SapI 1204 GAAGAGC GCTCTTC(1/4) SfcI 82 CTGCAG C^T[GA][CT]AG XcmI 512 CCATTAGCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 1752 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcsI 121 AAATTC [GA]^AATT[CT] 1681 AAATTC AocI 1471 CCTCAGG CC^T[ACGT]AGG 1639 CCTGAGG ApoI 121 AAATTC [GA]^AATT[CT] 1681 AAATTC BanI 301 GGTGCC G^G[CT][GA]CC 1161 GGTGCC BsaI 241 GAGACC GGTCTC(1/5) 1570 GAGACC BsmBI 919 CGTCTC CGTCTC(1/5) 1420 GAGACG BsmFI 444 GGGAC GGGAC(10/14) 155 GTCCC BspHI 216 TCATGA T^CATGA 1259 TCATGA BstYI 288 GGATCC [GA]^GATC[CT] 980 AGATCC Bsu36I 1471 CCTCAGG CC^T[ACGT]AGG 1639 CCTGAGG EaeI 953 TGGCCA [CT]^GGCC[GA] 1071 TGGCCG Eco81I 1471 CCTCAGG CC^T[ACGT]AGG 1639 CCTGAGG MaeII 14 ACGT A^CGT 918 ACGT MflI 288 GGATCC [GA]^GATC[CT] 980 AGATCC MslI 753 CATGGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1654 CACAGAAGTG PflMI 387 CCACAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1036 CCAAAATATGG PpuMI 221 AGGACCT [GA]G^G[AT]CC[CT] 359 AGGTCCT RcaI 216 TCATGA T^CATGA 1259 TCATGA ScaI 270 AGTACT AGT^ACT 1399 AGTACT TfiI 1142 GAATC G^A[AT]TC 1248 GAATC TspRI 974 CAGTG CA[GC]TG[ACGT][ACGT]^ 1707 CAGTG Van91I 387 CCACAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1036 CCAAAATATGG XhoII 288 GGATCC [GA]^GATC[CT] 980 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 73 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AspHI AvaII BbsI BbvI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MboI MboII MnlI MseI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI StyI TaqI Tru9I TseI Tsp509I TthHB8I