(Circular) (Six-base) MAPSORT of: pFN21AE2017.seq Check: 8049 from: 1 to: 6487 pFN21AE2017 6487 bp With 182 enzymes: SgfI * August 16, 2010 14:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2086 2262 2086 Size: 176 6311 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4297 278 Size: 53 83 186 936 2761 2468 Fragments arranged by size: 2761 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3614 3830 1937 Size: 1677 216 4594 Fragments arranged by size: 4594 1677 216 Acc65I G'GTAC_C Cuts at: 3808 3808 Size: 6487 AclI AA'CG_TT Cuts at: 1563 4614 4987 1563 Size: 3051 373 3063 Fragments arranged by size: 3063 3051 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2081 2229 3041 3372 3741 4550 5934 6202 Size: 449 148 812 331 369 809 1384 268 Cuts at: 6202 1632 Size: 1917 Fragments arranged by size: 1917 1384 812 809 449 369 331 268 148 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6290 AflIII A'CryG_T Cuts at: 1203 5386 6363 1203 Size: 4183 977 1327 Fragments arranged by size: 4183 1327 977 AgeI A'CCGG_T Cuts at: 5318 5318 Size: 6487 AhdI GACnn_n'nnGTC Cuts at: 5216 5216 Size: 6487 AleI CACnn'nnGTG Cuts at: 1166 3663 1166 Size: 2497 3990 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3145 1184 Size: 1961 4526 AlwNI CAG_nnn'CTG Cuts at: 4230 5802 4230 Size: 1572 4915 ApaLI G'TGCA_C Cuts at: 2919 4544 5700 2919 Size: 1625 1156 3706 Fragments arranged by size: 3706 1625 1156 ApoI r'AATT_y Cuts at: 1493 2126 3686 3796 4022 4174 6283 1493 Size: 633 1560 110 226 152 2109 1697 Fragments arranged by size: 2109 1697 1560 633 226 152 110 AseI AT'TA_AT Cuts at: 160 1032 5041 160 Size: 872 4009 1606 Fragments arranged by size: 4009 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6487 AvaI C'yCGr_G Cuts at: 1870 1943 3812 1870 Size: 73 1869 4545 Fragments arranged by size: 4545 1869 73 BamHI G'GATC_C Cuts at: 2293 3817 4184 2293 Size: 1524 367 4596 Fragments arranged by size: 4596 1524 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2306 3166 3808 5263 618 Size: 325 217 627 519 860 642 1455 1842 Fragments arranged by size: 1842 1455 860 642 627 519 325 217 BanII G_rGCy'C Cuts at: 1391 3019 3806 1391 Size: 1628 787 4072 Fragments arranged by size: 4072 1628 787 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3029 3378 3501 928 Size: 971 1130 349 123 3914 Fragments arranged by size: 3914 1130 971 349 123 BbvCI CC'TCA_GC Cuts at: 2361 2361 Size: 6487 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3135 3169 4678 4712 1903 Size: 34 1198 34 1509 34 3678 Fragments arranged by size: 3678 1509 1198 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2976 4379 5589 2976 Size: 1403 1210 3874 Fragments arranged by size: 3874 1403 1210 BclI T'GATC_A Cuts at: 1548 2505 1548 Size: 957 5530 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5098 136 Size: 107 122 71 631 605 3426 1525 Fragments arranged by size: 3426 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6482 6482 Size: 6487 BlpI GC'TnA_GC Cuts at: 3904 3904 Size: 6487 Bme1580I G_kGCm'C Cuts at: 1790 2923 3171 4548 5704 1790 Size: 1133 248 1377 1156 2573 Fragments arranged by size: 2573 1377 1156 1133 248 BmgBI CAC'GTC Cuts at: 1318 2924 1318 Size: 1606 4881 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2641 4218 5176 Size: 416 53 83 399 24 1224 1577 958 Cuts at: 5176 442 Size: 1753 Fragments arranged by size: 1753 1577 1224 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6487 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2821 2821 Size: 6487 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2367 2708 2717 2791 3255 3341 3393 5147 Size: 1128 341 9 74 464 86 52 1754 Cuts at: 5147 1239 Size: 2579 Fragments arranged by size: 2579 1754 1128 464 341 86 74 52 9 Bpu10I CC'TnA_GC Cuts at: 2361 2727 2361 Size: 366 6121 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4612 5477 5775 4612 Size: 865 298 5324 Fragments arranged by size: 5324 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2240 3569 5150 882 Size: 617 741 1329 1581 2219 Fragments arranged by size: 2219 1581 1329 741 617 BsaAI yAC'GTr Cuts at: 493 1204 2020 4278 493 Size: 711 816 2258 2702 Fragments arranged by size: 2702 2258 816 711 BsaBI GATnn'nnATC Cuts at: 2792 6481 2792 Size: 3689 2798 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4294 4676 5311 275 Size: 53 83 186 936 2761 382 635 1451 Fragments arranged by size: 2761 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2596 4920 5318 5592 5739 6150 2596 Size: 2324 398 274 147 411 2933 Fragments arranged by size: 2933 2324 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2315 2345 2846 2876 1178 Size: 30 165 30 912 30 501 30 4789 Fragments arranged by size: 4789 912 501 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2087 2117 2336 2364 2388 2714 3428 3437 Size: 901 30 219 28 24 326 714 9 Cuts at: 3437 3563 3662 3713 1186 Size: 126 99 51 3960 Fragments arranged by size: 3960 901 714 326 219 126 99 51 30 28 24 9 BseYI C'CCAG_C Cuts at: 2440 2446 2885 5690 6186 2440 Size: 6 439 2805 496 2741 Fragments arranged by size: 2805 2741 496 439 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2369 3002 3354 1163 Size: 573 633 633 352 4296 Fragments arranged by size: 4296 633 633 573 352 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2040 3937 4698 5726 1540 Size: 275 186 39 1897 761 1028 2301 Fragments arranged by size: 2301 1897 1028 761 275 186 39 BsiHKAI G_wGCw'C Cuts at: 2034 2115 2923 4548 4633 5704 2034 Size: 81 808 1625 85 1071 2817 Fragments arranged by size: 2817 1625 1071 808 85 81 BsmI GAATG_Cn' Cuts at: 3998 4091 3998 Size: 93 6394 BsmBI CGTCTCn'nnnn_ Cuts at: 2930 3419 2930 Size: 489 5998 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2034 2115 2923 3019 3171 3806 4548 Size: 399 244 81 808 96 152 635 742 Cuts at: 4548 4633 5704 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 808 742 635 399 244 152 96 85 81 BspEI T'CCGG_A Cuts at: 2596 6150 2596 Size: 3554 2933 BspHI T'CATG_A Cuts at: 2221 3264 4376 6106 2221 Size: 1043 1112 1730 2602 Fragments arranged by size: 2602 1730 1112 1043 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2086 2262 2865 3842 844 Size: 1242 176 603 977 3489 Fragments arranged by size: 3489 1242 977 603 176 BsrBI CCG'CTC Cuts at: 1394 1823 4374 1394 Size: 429 2551 3507 Fragments arranged by size: 3507 2551 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3282 4982 5156 65 Size: 1183 2034 1700 174 1396 Fragments arranged by size: 2034 1700 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3703 5131 5318 1700 Size: 110 1893 1428 187 2869 Fragments arranged by size: 2869 1893 1428 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6487 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6487 BssSI C'ACGA_G Cuts at: 3172 4547 5559 3172 Size: 1375 1012 4100 Fragments arranged by size: 4100 1375 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6487 BstBI TT'CG_AA Cuts at: 5291 5291 Size: 6487 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6487 BstXI CCAn_nnnn'nTGG Cuts at: 2654 2764 2815 3665 2654 Size: 110 51 850 5476 Fragments arranged by size: 5476 850 110 51 BstYI r'GATC_y Cuts at: 1972 2293 2985 3817 4184 4569 4586 6038 6124 Size: 321 692 832 367 385 17 1452 86 Cuts at: 6124 6136 6482 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 832 692 385 367 346 321 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3477 3645 3477 Size: 168 6319 BtgI C'CryG_G Cuts at: 513 1065 2004 2757 513 Size: 552 939 753 4243 Fragments arranged by size: 4243 939 753 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2914 487 Size: 683 56 1688 4060 Fragments arranged by size: 4060 1688 683 56 BtsI GCAGTG_nn' Cuts at: 3718 4003 4797 4817 3718 Size: 285 794 20 5388 Fragments arranged by size: 5388 794 285 20 ClaI AT'CG_AT Cuts at: 1554 3150 1554 Size: 1596 4891 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3055 3090 529 Size: 35 2491 35 3926 Fragments arranged by size: 3926 2491 35 35 DraI TTT'AAA Cuts at: 3791 3791 Size: 6487 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2419 5494 809 Size: 507 1103 3075 1802 Fragments arranged by size: 3075 1802 1103 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2958 3076 3934 Size: 54 1425 50 275 18 1128 118 858 Cuts at: 3934 4823 6452 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1128 889 858 275 118 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3934 1537 Size: 275 2122 4090 Fragments arranged by size: 4090 2122 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3204 3567 3717 4417 1353 Size: 1851 363 150 700 3423 Fragments arranged by size: 3423 1851 700 363 150 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2222 5074 5448 5594 387 Size: 1064 138 633 2852 374 146 1280 Fragments arranged by size: 2852 1280 1064 633 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2081 2229 2367 2708 2717 2791 3041 Size: 393 449 148 138 341 9 74 250 Cuts at: 3041 3255 3341 3372 3393 3741 4550 5147 5934 Size: 214 86 31 21 348 809 597 787 Cuts at: 5934 6202 1239 Size: 268 1524 Fragments arranged by size: 1524 809 787 597 449 393 348 341 268 250 214 148 138 86 74 31 21 9 EcoO109I rG'GnC_Cy Cuts at: 2227 2365 3004 2227 Size: 138 639 5710 Fragments arranged by size: 5710 639 138 EcoRI G'AATT_C Cuts at: 3796 4174 3796 Size: 378 6109 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3358 3358 Size: 6487 FspI TGC'GCA Cuts at: 4993 4993 Size: 6487 HaeII r_GCGC'y Cuts at: 1678 2060 5634 1678 Size: 382 3574 2531 Fragments arranged by size: 3574 2531 382 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3143 3175 3203 3235 3550 Size: 32 135 32 1571 32 28 32 315 Cuts at: 3550 3582 5128 5160 5202 5234 1373 Size: 32 1546 32 42 32 2626 Fragments arranged by size: 2626 1571 1546 315 135 42 32 32 32 32 32 32 32 28 HincII GTy'rAC Cuts at: 669 1657 1938 3831 4077 669 Size: 988 281 1893 246 3079 Fragments arranged by size: 3079 1893 988 281 246 HindIII A'AGCT_T Cuts at: 748 3191 748 Size: 2443 4044 HpaI GTT'AAC Cuts at: 4077 4077 Size: 6487 KpnI G_GTAC'C Cuts at: 3812 3812 Size: 6487 MfeI C'AATT_G Cuts at: 4086 4086 Size: 6487 MluI A'CGCG_T Cuts at: 6363 6363 Size: 6487 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5601 5785 1305 Size: 4296 184 2007 Fragments arranged by size: 4296 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2960 10 Size: 54 1425 343 1128 3537 Fragments arranged by size: 3537 1425 1128 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2762 3663 4445 4804 4963 518 Size: 648 1596 901 782 359 159 2042 Fragments arranged by size: 2042 1596 901 782 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2440 2536 3086 3581 3903 4580 5728 5973 Size: 834 96 550 495 322 677 1148 245 Cuts at: 5973 1606 Size: 2120 Fragments arranged by size: 2120 1148 834 677 550 495 322 245 96 NcoI C'CATG_G Cuts at: 513 1065 2004 2757 513 Size: 552 939 753 4243 Fragments arranged by size: 4243 939 753 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6487 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6487 NotI GC'GGCC_GC Cuts at: 3934 3934 Size: 6487 NspI r_CATG'y Cuts at: 1132 1229 2939 3845 5390 1132 Size: 97 1710 906 1545 2229 Fragments arranged by size: 2229 1710 1545 906 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6487 PciI A'CATG_T Cuts at: 5386 5386 Size: 6487 PflMI CCAn_nnn'nTGG Cuts at: 1108 2398 3047 1108 Size: 1290 649 4548 Fragments arranged by size: 4548 1290 649 PfoI T'CCnGG_A Cuts at: 2526 2526 Size: 6487 PmeI GTTT'AAAC Cuts at: 3791 3791 Size: 6487 PmlI CAC'GTG Cuts at: 2020 2020 Size: 6487 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4556 4588 6069 6101 4556 Size: 32 1481 32 4942 Fragments arranged by size: 4942 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2227 2365 2227 Size: 138 6349 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6487 PsiI TTA'TAA Cuts at: 4057 4057 Size: 6487 PstI C_TGCA'G Cuts at: 830 2091 3839 830 Size: 1261 1748 3478 Fragments arranged by size: 3478 1748 1261 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6487 PvuII CAG'CTG Cuts at: 2440 2536 3086 2440 Size: 96 550 5841 Fragments arranged by size: 5841 550 96 SalI G'TCGA_C Cuts at: 1936 3829 1936 Size: 1893 4594 SapI GCTCTTCn'nnn_ Cuts at: 3204 3204 Size: 6487 SbfI CC_TGCA'GG Cuts at: 3839 3839 Size: 6487 ScaI AGT'ACT Cuts at: 2277 3406 4735 6447 2277 Size: 1129 1329 1712 2317 Fragments arranged by size: 2317 1712 1329 1129 SfcI C'TryA_G Cuts at: 826 1045 2087 3835 4970 5651 5842 826 Size: 219 1042 1748 1135 681 191 1471 Fragments arranged by size: 1748 1471 1135 1042 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6487 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6487 SmaI CCC'GGG Cuts at: 1872 3814 1872 Size: 1942 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4591 5492 5754 820 Size: 197 926 2648 901 262 1553 Fragments arranged by size: 2648 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4278 493 Size: 3785 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6487 SphI G_CATG'C Cuts at: 1132 3845 1132 Size: 2713 3774 SspI AAT'ATT Cuts at: 5 52 4411 5 Size: 47 4359 2081 Fragments arranged by size: 4359 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6487 StyI C'CwwG_G Cuts at: 513 1065 2004 2184 2721 2757 2989 3543 3621 Size: 552 939 180 537 36 232 554 78 Cuts at: 3621 3926 513 Size: 305 3074 Fragments arranged by size: 3074 939 554 552 537 305 232 180 78 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2429 3634 4514 4531 4684 4869 5314 Size: 1255 849 1205 880 17 153 185 445 Cuts at: 5314 325 Size: 1498 Fragments arranged by size: 1498 1255 1205 880 849 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2275 3404 4733 Size: 274 80 33 51 1443 298 1129 1329 Cuts at: 4733 6445 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1329 1129 298 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2243 2275 2444 2476 3580 3612 6447 6479 2243 Size: 32 169 32 1104 32 2835 32 2251 Fragments arranged by size: 2835 2251 1104 169 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6487 XbaI T'CTAG_A Cuts at: 3823 3823 Size: 6487 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2524 2524 Size: 6487 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6487 XmaI C'CCGG_G Cuts at: 1870 3812 1870 Size: 1942 4545 XmnI GAAnn'nnTTC Cuts at: 3761 4616 3761 Size: 855 5632 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4295 276 Size: 53 83 186 936 2761 2468 Fragments arranged by size: 2761 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI