################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII AclI AcsI AcyI AflII AgeI Alw44I AlwNI AocI Aor51HI ApaLI ApoI AscI AspI AvaI AviII AvrII BalI BamHI BbeI BbsI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KspI MamI MfeI MflI MluI MluNI MscI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI PshAI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### Acc65I 919 GGTACC G^GTACC AccI 1174 GTCTAC GT^[AC][GT]AC AccII 652 CGCG CG^CG AccIII 368 TCCGGA T^CCGGA AfaI 920 GTAC GT^AC AflIII 872 ACGTGT A^C[GA][CT]GT AhdI 604 GACAGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 177 GATCC GGATC(4/5) AseI 228 ATTAAT AT^TAAT AsnI 228 ATTAAT AT^TAAT Asp700I 543 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 919 GGTACC G^GTACC AspEI 604 GACAGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 565 GAGCTC G[AT]GC[AT]^C BbrPI 388 CACGTG CAC^GTG BbvCI 481 CCTCAGC CCTCAGC(-5/-2) BcnI 222 CCGGG CC^[GC]GG BfaI 32 CTAG C^TAG BsaAI 388 CACGTG [CT]AC^GT[GA] BsaWI 368 TCCGGA [AT]^CCGG[AT] BseAI 368 TCCGGA T^CCGGA BseRI 206 GAGGAG GAGGAG(10/8) BsiHKAI 565 GAGCTC G[AT]GC[AT]^C BspEI 368 TCCGGA T^CCGGA BsrFI 343 GCCGGC [GA]^CCGG[CT] BstUI 652 CGCG CG^CG BstXI 10 CCAGCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 343 GCCGGC [GA]^CCGG[CT] DpnII 177 GATC ^GATC DraIII 487 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1033 GACCTGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 146 CGGCCA [CT]^GGCC[GA] Eam1105I 604 GACAGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC HgaI 51 GACGC GACGC(5/10) KpnI 919 GGTACC GGTAC^C MaeI 32 CTAG C^TAG MboI 177 GATC ^GATC MroI 368 TCCGGA T^CCGGA MslI 964 CACAGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 652 CGCG CG^CG NaeI 343 GCCGGC GCC^GGC NciI 222 CCGGG CC^[GC]GG NdeII 177 GATC ^GATC NgoMIV 343 GCCGGC G^CCGGC PflMI 191 CCATCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 388 CACGTG CAC^GTG PmlI 388 CACGTG CAC^GTG PpuMI 707 GGGACCC [GA]G^G[AT]CC[CT] PshBI 228 ATTAAT AT^TAAT PstI 97 CTGCAG CTGCA^G RsaI 920 GTAC GT^AC SacI 565 GAGCTC GAGCT^C SapI 91 GAAGAGC GCTCTTC(1/4) Sau3AI 177 GATC ^GATC SmlI 668 CTCAAG C^T[CT][GA]AG TfiI 105 GATTC G^A[AT]TC Tsp509I 227 AATT ^AATT Van91I 191 CCATCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1136 CCAAGGTGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 543 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### BpmI 137 CTGGAG CTGGAG(16/14) 404 CTGGAG BsaI 739 GAGACC GGTCTC(1/5) 1041 GGTCTC BsgI 154 CTGCAC GTGCAG(16/14) 159 CTGCAC EarI 713 CTCTTC CTCTTC(1/4) 91 GAAGAG FauI 595 CCCGC CCCGC(4/6) 650 CCCGC Ksp632I 713 CTCTTC CTCTTC(1/4) 91 GAAGAG MboII 91 GAAGA GAAGA(8/7) 714 TCTTC MseI 229 TTAA T^TAA 398 TTAA PvuII 134 CAGCTG CAG^CTG 847 CAGCTG SexAI 797 ACCAGGT A^CC[AT]GGT 853 ACCTGGT Tru9I 229 TTAA T^TAA 398 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AciI AluI ApaI AvaII BanI BanII BbvI BmyI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DraII DsaI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeII MaeIII MnlI MspA1I MspI MvaI MwoI NlaIII NlaIV PleI Sau96I ScrFI SfaNI SfcI StyI TseI Tsp45I TspRI