(Circular) (Six-base) MAPSORT of: pFN21AE1877.seq Check: 6405 from: 1 to: 5899 pFN21AE1877 5899 bp With 182 enzymes: SgfI * August 16, 2010 14:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2095 2516 2825 2999 2095 Size: 421 309 174 4995 Fragments arranged by size: 4995 421 309 174 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3709 278 Size: 53 83 186 936 2173 2468 Fragments arranged by size: 2468 2173 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3180 3242 1937 Size: 1243 62 4594 Fragments arranged by size: 4594 1243 62 Acc65I G'GTAC_C Cuts at: 2924 3220 2924 Size: 296 5603 AclI AA'CG_TT Cuts at: 1563 4026 4399 1563 Size: 2463 373 3063 Fragments arranged by size: 3063 2463 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3062 3962 5346 5614 1632 Size: 1430 900 1384 268 1917 Fragments arranged by size: 1917 1430 1384 900 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5702 AflIII A'CryG_T Cuts at: 1203 2877 4798 5775 1203 Size: 1674 1921 977 1327 Fragments arranged by size: 1921 1674 1327 977 AgeI A'CCGG_T Cuts at: 4730 4730 Size: 5899 AhdI GACnn_n'nnGTC Cuts at: 2614 4628 2614 Size: 2014 3885 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5899 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5899 AlwNI CAG_nnn'CTG Cuts at: 3642 5214 3642 Size: 1572 4327 ApaI G_GGCC'C Cuts at: 2196 2468 3140 2196 Size: 272 672 4955 Fragments arranged by size: 4955 672 272 ApaLI G'TGCA_C Cuts at: 3956 5112 3956 Size: 1156 4743 ApoI r'AATT_y Cuts at: 1493 3208 3434 3586 5695 1493 Size: 1715 226 152 2109 1697 Fragments arranged by size: 2109 1715 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 2234 4453 160 Size: 872 1202 2219 1606 Fragments arranged by size: 2219 1606 1202 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5899 AvaI C'yCGr_G Cuts at: 1870 1943 3224 1870 Size: 73 1281 4545 Fragments arranged by size: 4545 1281 73 BamHI G'GATC_C Cuts at: 3229 3596 3229 Size: 367 5532 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2351 2417 2924 3220 4675 Size: 325 217 627 564 66 507 296 1455 Cuts at: 4675 618 Size: 1842 Fragments arranged by size: 1842 1455 627 564 507 325 296 217 66 BanII G_rGCy'C Cuts at: 1391 2196 2387 2468 2574 3074 3140 3218 1391 Size: 805 191 81 106 500 66 78 4072 Fragments arranged by size: 4072 805 500 191 106 81 78 66 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4928 BbvCI CC'TCA_GC Cuts at: 2487 2487 Size: 5899 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4090 4124 1903 Size: 34 50 34 2069 34 3678 Fragments arranged by size: 3678 2069 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3791 5001 3791 Size: 1210 4689 BclI T'GATC_A Cuts at: 1548 1548 Size: 5899 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4510 136 Size: 107 122 71 631 605 2838 1525 Fragments arranged by size: 2838 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5894 5894 Size: 5899 BlpI GC'TnA_GC Cuts at: 3316 3316 Size: 5899 Bme1580I G_kGCm'C Cuts at: 1790 2196 2468 3140 3960 5116 1790 Size: 406 272 672 820 1156 2573 Fragments arranged by size: 2573 1156 820 672 406 272 BmgBI CAC'GTC Cuts at: 1318 2698 1318 Size: 1380 4519 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3630 4588 442 Size: 416 53 83 399 24 2213 958 1753 Fragments arranged by size: 2213 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5899 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3088 3088 Size: 5899 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2163 2430 4559 1239 Size: 924 267 2129 2579 Fragments arranged by size: 2579 2129 924 267 Bpu10I CC'TnA_GC Cuts at: 2487 2487 Size: 5899 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2658 4024 4889 5187 2658 Size: 1366 865 298 3370 Fragments arranged by size: 3370 1366 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2738 3052 4562 882 Size: 617 1239 314 1510 2219 Fragments arranged by size: 2219 1510 1239 617 314 BsaAI yAC'GTr Cuts at: 493 1204 2395 3690 493 Size: 711 1191 1295 2702 Fragments arranged by size: 2702 1295 1191 711 BsaBI GATnn'nnATC Cuts at: 5893 5893 Size: 5899 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3706 4088 4723 275 Size: 53 83 186 936 2173 382 635 1451 Fragments arranged by size: 2173 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2373 4332 4730 5004 5151 5562 2373 Size: 1959 398 274 147 411 2710 Fragments arranged by size: 2710 1959 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2390 2420 2598 2628 1178 Size: 30 165 30 987 30 178 30 4449 Fragments arranged by size: 4449 987 178 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2226 1186 Size: 1040 4859 BseYI C'CCAG_C Cuts at: 2065 2338 3091 5102 5598 2065 Size: 273 753 2011 496 2366 Fragments arranged by size: 2366 2011 753 496 273 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2144 2149 1163 Size: 573 408 5 4913 Fragments arranged by size: 4913 573 408 5 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3349 4110 5138 1540 Size: 275 186 1348 761 1028 2301 Fragments arranged by size: 2301 1348 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2574 3960 4045 5116 2574 Size: 1386 85 1071 3357 Fragments arranged by size: 3357 1386 1071 85 BsmI GAATG_Cn' Cuts at: 3410 3503 3410 Size: 93 5806 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2196 2387 2468 2574 3074 3140 3218 Size: 399 406 191 81 106 500 66 78 Cuts at: 3218 3960 4045 5116 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 500 406 399 191 106 85 81 78 66 BspEI T'CCGG_A Cuts at: 2373 5562 2373 Size: 3189 2710 BspHI T'CATG_A Cuts at: 3788 5518 3788 Size: 1730 4169 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2095 2516 2825 2999 3254 844 Size: 1251 421 309 174 255 3489 Fragments arranged by size: 3489 1251 421 309 255 174 BsrBI CCG'CTC Cuts at: 1394 1823 3786 1394 Size: 429 1963 3507 Fragments arranged by size: 3507 1963 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4394 4568 65 Size: 1183 3146 174 1396 Fragments arranged by size: 3146 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2348 4543 4730 1700 Size: 110 538 2195 187 2869 Fragments arranged by size: 2869 2195 538 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5899 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5899 BssSI C'ACGA_G Cuts at: 3959 4971 3959 Size: 1012 4887 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5899 BstBI TT'CG_AA Cuts at: 4703 4703 Size: 5899 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5899 BstXI CCAn_nnnn'nTGG Cuts at: 2022 2022 Size: 5899 BstYI r'GATC_y Cuts at: 1972 3229 3596 3981 3998 5450 5536 5548 5894 Size: 1257 367 385 17 1452 86 12 346 Cuts at: 5894 1972 Size: 1977 Fragments arranged by size: 1977 1452 1257 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2264 2454 2518 513 Size: 552 1199 190 64 3894 Fragments arranged by size: 3894 1199 552 190 64 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5160 Fragments arranged by size: 5160 683 56 BtsI GCAGTG_nn' Cuts at: 2161 3415 4209 4229 2161 Size: 1254 794 20 3831 Fragments arranged by size: 3831 1254 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5899 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2748 2783 529 Size: 35 2184 35 3645 Fragments arranged by size: 3645 2184 35 35 DraI TTT'AAA Cuts at: 3203 3203 Size: 5899 DraIII CAC_nnn'GTG Cuts at: 2497 2497 Size: 5899 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3044 4906 809 Size: 507 1728 1862 1802 Fragments arranged by size: 1862 1802 1728 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2151 3346 4235 Size: 54 1425 50 275 18 321 1195 889 Cuts at: 4235 5864 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1195 889 321 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3346 1537 Size: 275 1534 4090 Fragments arranged by size: 4090 1534 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2091 2724 3829 1353 Size: 738 633 1105 3423 Fragments arranged by size: 3423 1105 738 633 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4486 4860 5006 387 Size: 1064 138 2897 374 146 1280 Fragments arranged by size: 2897 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2572 2572 Size: 5899 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2163 2430 3062 3962 4559 5346 5614 Size: 393 531 267 632 900 597 787 268 Cuts at: 5614 1239 Size: 1524 Fragments arranged by size: 1524 900 787 632 597 531 393 268 267 EcoO109I rG'GnC_Cy Cuts at: 2192 2465 2713 3137 2192 Size: 273 248 424 4954 Fragments arranged by size: 4954 424 273 248 EcoRI G'AATT_C Cuts at: 3208 3586 3208 Size: 378 5521 FspI TGC'GCA Cuts at: 4405 4405 Size: 5899 HaeII r_GCGC'y Cuts at: 1678 5046 1678 Size: 3368 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2390 2422 2600 2632 2654 Size: 32 135 32 818 32 178 32 22 Cuts at: 2654 2686 4540 4572 4614 4646 1373 Size: 32 1854 32 42 32 2626 Fragments arranged by size: 2626 1854 818 178 135 42 32 32 32 32 32 32 32 22 HincII GTy'rAC Cuts at: 669 1657 1938 3243 3489 669 Size: 988 281 1305 246 3079 Fragments arranged by size: 3079 1305 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5899 HpaI GTT'AAC Cuts at: 3489 3489 Size: 5899 KpnI G_GTAC'C Cuts at: 2928 3224 2928 Size: 296 5603 MfeI C'AATT_G Cuts at: 3498 3498 Size: 5899 MluI A'CGCG_T Cuts at: 5775 5775 Size: 5899 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2844 5013 5197 1305 Size: 1539 2169 184 2007 Fragments arranged by size: 2169 2007 1539 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4077 Fragments arranged by size: 4077 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2973 3857 4216 4375 518 Size: 648 843 964 884 359 159 2042 Fragments arranged by size: 2042 964 884 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2141 2854 3020 3315 3992 5140 5385 1606 Size: 535 713 166 295 677 1148 245 2120 Fragments arranged by size: 2120 1148 713 677 535 295 245 166 NaeI GCC'GGC Cuts at: 2350 2350 Size: 5899 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5347 NdeI CA'TA_TG Cuts at: 387 387 Size: 5899 NgoMIV G'CCGG_C Cuts at: 2348 2348 Size: 5899 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5899 NotI GC'GGCC_GC Cuts at: 3346 3346 Size: 5899 NspI r_CATG'y Cuts at: 1132 1229 3257 4802 1132 Size: 97 2028 1545 2229 Fragments arranged by size: 2229 2028 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5899 PciI A'CATG_T Cuts at: 4798 4798 Size: 5899 PflMI CCAn_nnn'nTGG Cuts at: 1108 2202 1108 Size: 1094 4805 PfoI T'CCnGG_A Cuts at: 2882 2882 Size: 5899 PmeI GTTT'AAAC Cuts at: 3203 3203 Size: 5899 PmlI CAC'GTG Cuts at: 2395 2395 Size: 5899 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3968 4000 5481 5513 3968 Size: 32 1481 32 4354 Fragments arranged by size: 4354 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2713 2713 Size: 5899 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5899 PsiI TTA'TAA Cuts at: 3469 3469 Size: 5899 PspOMI G'GGCC_C Cuts at: 2192 2464 3136 2192 Size: 272 672 4955 Fragments arranged by size: 4955 672 272 PstI C_TGCA'G Cuts at: 830 2106 3251 830 Size: 1276 1145 3478 Fragments arranged by size: 3478 1276 1145 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5899 PvuII CAG'CTG Cuts at: 2141 2854 2141 Size: 713 5186 SacI G_AGCT'C Cuts at: 2574 2574 Size: 5899 SalI G'TCGA_C Cuts at: 1936 3241 1936 Size: 1305 4594 SanDI GG'GwC_CC Cuts at: 2713 2713 Size: 5899 SapI GCTCTTCn'nnn_ Cuts at: 2091 2091 Size: 5899 SbfI CC_TGCA'GG Cuts at: 3251 3251 Size: 5899 ScaI AGT'ACT Cuts at: 4147 5859 4147 Size: 1712 4187 SexAI A'CCwGG_T Cuts at: 2802 2858 2802 Size: 56 5843 SfcI C'TryA_G Cuts at: 826 1045 2102 2620 2849 3247 4382 5063 5254 Size: 219 1057 518 229 398 1135 681 191 Cuts at: 5254 826 Size: 1471 Fragments arranged by size: 1471 1135 1057 681 518 398 229 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5899 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5899 SmaI CCC'GGG Cuts at: 1872 3226 1872 Size: 1354 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2673 4003 4904 5166 820 Size: 197 926 730 1330 901 262 1553 Fragments arranged by size: 1553 1330 926 901 730 262 197 SnaBI TAC'GTA Cuts at: 493 3690 493 Size: 3197 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5899 SphI G_CATG'C Cuts at: 1132 3257 1132 Size: 2125 3774 SspI AAT'ATT Cuts at: 5 52 3823 5 Size: 47 3771 2081 Fragments arranged by size: 3771 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5899 StyI C'CwwG_G Cuts at: 513 1065 2640 2766 3141 3338 513 Size: 552 1575 126 375 197 3074 Fragments arranged by size: 3074 1575 552 375 197 126 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2545 2776 3926 3943 4096 4281 4726 Size: 1255 965 231 1150 17 153 185 445 Cuts at: 4726 325 Size: 1498 Fragments arranged by size: 1498 1255 1150 965 445 231 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4145 5857 96 Size: 274 80 33 51 1443 2168 1712 138 Fragments arranged by size: 2168 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2598 2630 5859 5891 2598 Size: 32 3229 32 2606 Fragments arranged by size: 3229 2606 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5899 XbaI T'CTAG_A Cuts at: 3235 3235 Size: 5899 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3148 3148 Size: 5899 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5899 XmaI C'CCGG_G Cuts at: 1870 3224 1870 Size: 1354 4545 XmnI GAAnn'nnTTC Cuts at: 2552 4028 2552 Size: 1476 4423 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3707 276 Size: 53 83 186 936 2173 2468 Fragments arranged by size: 2468 2173 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SacII SfiI SfoI SrfI SwaI