################################## # NO CUT ENZYMES ( 131 enzymes) # ################################## AatII AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspHI AviII AvrII BamHI BbeI BbrPI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstYI BstZ17I Bsu36I CelII ClaI CpoI DpnII DraI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoRI EcoT22I FbaI FseI FspI Hin1I HindIII HpaI KasI MamI MboI MfeI MflI MluI MroI MseI MunI NarI NdeI NdeII NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SalI Sau3AI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SspBI SspI StuI SwaI TaqI TfiI Tru9I Tsp509I TthHB8I XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### Acc65I 113 GGTACC G^GTACC Aor51HI 1329 AGCGCT AGC^GCT Asp718I 113 GGTACC G^GTACC AspI 772 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 103 CCCGAG C^[CT]CG[GA]G BanI 113 GGTACC G^G[CT][GA]CC BfaI 862 CTAG C^TAG BsaWI 481 TCCGGT [AT]^CCGG[AT] BsoBI 103 CCCGAG C^[CT]CG[GA]G BsrFI 392 GCCGGC [GA]^CCGG[CT] Cfr10I 392 GCCGGC [GA]^CCGG[CT] Eco47III 1329 AGCGCT AGC^GCT EcoNI 1161 CCTCTTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 513 GATATC GAT^ATC EcoT14I 1136 CCATGG C^C[AT][AT]GG HinfI 437 GACTC G^A[ACGT]TC KpnI 113 GGTACC GGTAC^C KspI 1376 CCGCGG CCGC^GG MaeI 862 CTAG C^TAG NaeI 392 GCCGGC GCC^GGC NcoI 1136 CCATGG C^CATGG NgoMIV 392 GCCGGC G^CCGGC NspI 947 GCATGC [GA]CATG^[CT] PflFI 772 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 734 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 437 GACTC GAGTC(4/5) PstI 824 CTGCAG CTGCA^G SacII 1376 CCGCGG CCGC^GG SapI 1365 GCTCTTC GCTCTTC(1/4) SbfI 823 CCTGCAGG CCTGCA^GG SexAI 773 ACCAGGT A^CC[AT]GGT SfcI 824 CTGCAG C^T[GA][CT]AG SphI 947 GCATGC GCATG^C Sse8387I 823 CCTGCAGG CCTGCA^GG StyI 1136 CCATGG C^C[AT][AT]GG Tth111I 772 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 734 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 861 TCTAGA T^CTAGA XcmI 416 CCACCTTCTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AlwNI 735 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG 1218 CAGCGGCTG ApaI 160 GGGCCC GGGCC^C 819 GGGCCC BcnI 598 CCCGG CC^[GC]GG 888 CCCGG BglI 457 GCCCTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 821 GCCCTGCAGGC BpmI 721 CTCCAG CTGGAG(16/14) 625 CTGGAG BsmFI 1108 GGGAC GGGAC(10/14) 618 GTCCC BspHI 386 TCATGA T^CATGA 686 TCATGA BsrI 1175 ACTGG ACTGG(1/-1) 991 CCAGT BstF5I 141 CATCC GGATG(2/0) 792 CATCC FokI 141 CATCC GGATG(9/13) 792 CATCC HaeII 1279 AGCGCC [GA]GCGC^[CT] 1329 AGCGCT MaeII 1235 ACGT A^CGT 1355 ACGT NciI 598 CCCGG CC^[GC]GG 888 CCCGG PpuMI 1091 GGGTCCT [GA]G^G[AT]CC[CT] 1108 GGGACCT RcaI 386 TCATGA T^CATGA 686 TCATGA ######################################### # 3 OR MORE CUT ENZYMES ( 73 enzymes) # ######################################### AccII AciI AfaI AluI AvaII BalI BanII BbsI BbvCI BbvI BmyI BpuAI BsaI BsaJI BseRI BsgI BsiYI BslI BsmAI Bsp1286I BspMI BssKI BssSI BstEII BstNI BstPI BstUI BstXI Cac8I CfoI Cfr13I DdeI DraII DsaI EaeI EarI EcoO109I EcoO65I EcoRII FauI Fnu4HI HaeIII HapII HgaI HhaI HinP1I HincII HindII HpaII HphI ItaI Ksp632I MaeIII MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NlaIII NlaIV PvuII RsaI Sau96I ScrFI SfaNI TseI Tsp45I TspRI