(Circular) (Six-base) MAPSORT of: pFN21AE6460.seq Check: 1670 from: 1 to: 6112 pFN21AE6460 6112 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2031 2267 2031 Size: 236 5876 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3922 278 Size: 53 83 186 936 2386 2468 Fragments arranged by size: 2468 2386 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3455 1937 Size: 1518 4594 Acc65I G'GTAC_C Cuts at: 2118 3433 2118 Size: 1315 4797 AclI AA'CG_TT Cuts at: 1563 4239 4612 1563 Size: 2676 373 3063 Fragments arranged by size: 3063 2676 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2355 4175 5559 5827 1632 Size: 723 1820 1384 268 1917 Fragments arranged by size: 1917 1820 1384 723 268 AfeI AGC'GCT Cuts at: 3336 3336 Size: 6112 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5915 AflIII A'CryG_T Cuts at: 1203 5011 5988 1203 Size: 3808 977 1327 Fragments arranged by size: 3808 1327 977 AgeI A'CCGG_T Cuts at: 4943 4943 Size: 6112 AhdI GACnn_n'nnGTC Cuts at: 4841 4841 Size: 6112 AleI CACnn'nnGTG Cuts at: 1166 3183 1166 Size: 2017 4095 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2902 1184 Size: 1718 4394 AlwNI CAG_nnn'CTG Cuts at: 2745 3228 3855 5427 2745 Size: 483 627 1572 3430 Fragments arranged by size: 3430 1572 627 483 ApaI G_GGCC'C Cuts at: 2169 2828 2169 Size: 659 5453 ApaLI G'TGCA_C Cuts at: 4169 5325 4169 Size: 1156 4956 ApoI r'AATT_y Cuts at: 1493 3421 3647 3799 5908 1493 Size: 1928 226 152 2109 1697 Fragments arranged by size: 2109 1928 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4666 160 Size: 872 3634 1606 Fragments arranged by size: 3634 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6112 AvaI C'yCGr_G Cuts at: 1870 1943 2108 3437 1870 Size: 73 165 1329 4545 Fragments arranged by size: 4545 1329 165 73 BamHI G'GATC_C Cuts at: 3442 3809 3442 Size: 367 5745 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2118 3433 4888 618 Size: 325 217 627 331 1315 1455 1842 Fragments arranged by size: 1842 1455 1315 627 331 325 217 BanII G_rGCy'C Cuts at: 1391 2051 2073 2169 2486 2828 3158 3323 3431 Size: 660 22 96 317 342 330 165 108 Cuts at: 3431 1391 Size: 4072 Fragments arranged by size: 4072 660 342 330 317 165 108 96 22 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2029 2662 2769 928 Size: 971 130 633 107 4271 Fragments arranged by size: 4271 971 633 130 107 BbvCI CC'TCA_GC Cuts at: 3023 3233 3275 3023 Size: 210 42 5860 Fragments arranged by size: 5860 210 42 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4303 4337 1903 Size: 34 2366 34 3678 Fragments arranged by size: 3678 2366 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4004 5214 4004 Size: 1210 4902 BclI T'GATC_A Cuts at: 1548 1548 Size: 6112 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2468 2832 4723 Size: 107 122 71 631 605 796 364 1891 Cuts at: 4723 136 Size: 1525 Fragments arranged by size: 1891 1525 796 631 605 364 122 107 71 BglII A'GATC_T Cuts at: 6107 6107 Size: 6112 BlpI GC'TnA_GC Cuts at: 3529 3529 Size: 6112 Bme1580I G_kGCm'C Cuts at: 1790 2169 2223 2828 4173 5329 1790 Size: 379 54 605 1345 1156 2573 Fragments arranged by size: 2573 1345 1156 605 379 54 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6112 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3190 3843 4801 Size: 416 53 83 399 24 1773 653 958 Cuts at: 4801 442 Size: 1753 Fragments arranged by size: 1773 1753 958 653 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6112 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3356 3356 Size: 6112 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2651 2711 4772 1239 Size: 1412 60 2061 2579 Fragments arranged by size: 2579 2061 1412 60 Bpu10I CC'TnA_GC Cuts at: 3023 3233 3275 3023 Size: 210 42 5860 Fragments arranged by size: 5860 210 42 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4237 5102 5400 4237 Size: 865 298 4949 Fragments arranged by size: 4949 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2495 3061 3084 4775 882 Size: 617 996 566 23 1691 2219 Fragments arranged by size: 2219 1691 996 617 566 23 BsaAI yAC'GTr Cuts at: 493 1204 3903 493 Size: 711 2699 2702 Fragments arranged by size: 2702 2699 711 BsaBI GATnn'nnATC Cuts at: 6106 6106 Size: 6112 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3919 4301 4936 275 Size: 53 83 186 936 2386 382 635 1451 Fragments arranged by size: 2386 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2486 4545 4943 5217 5364 5775 2486 Size: 2059 398 274 147 411 2823 Fragments arranged by size: 2823 2059 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2111 2141 1178 Size: 30 165 30 708 30 5149 Fragments arranged by size: 5149 708 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2669 2759 2885 1186 Size: 1483 90 126 4413 Fragments arranged by size: 4413 1483 126 90 BseYI C'CCAG_C Cuts at: 2201 2239 2745 3253 3316 5315 5811 2201 Size: 38 506 508 63 1999 496 2502 Fragments arranged by size: 2502 1999 508 506 496 63 38 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2347 2987 3332 3383 1163 Size: 573 611 640 345 51 3892 Fragments arranged by size: 3892 640 611 573 345 51 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3562 4323 5351 1540 Size: 275 186 1561 761 1028 2301 Fragments arranged by size: 2301 1561 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4173 4258 5329 4173 Size: 85 1071 4956 Fragments arranged by size: 4956 1071 85 BsmI GAATG_Cn' Cuts at: 3623 3716 3623 Size: 93 6019 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2051 2073 2169 2223 2486 2828 3158 Size: 399 261 22 96 54 263 342 330 Cuts at: 3158 3323 3431 4173 4258 5329 1391 Size: 165 108 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 342 330 263 261 165 108 96 85 54 22 BspEI T'CCGG_A Cuts at: 5775 5775 Size: 6112 BspHI T'CATG_A Cuts at: 2391 2691 4001 5731 2391 Size: 300 1310 1730 2772 Fragments arranged by size: 2772 1730 1310 300 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2031 2130 2267 2507 3125 3467 844 Size: 1187 99 137 240 618 342 3489 Fragments arranged by size: 3489 1187 618 342 240 137 99 BsrBI CCG'CTC Cuts at: 1394 1823 3999 1394 Size: 429 2176 3507 Fragments arranged by size: 3507 2176 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4607 4781 65 Size: 1183 3359 174 1396 Fragments arranged by size: 3359 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2397 4756 4943 1700 Size: 110 587 2359 187 2869 Fragments arranged by size: 2869 2359 587 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6112 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6112 BssSI C'ACGA_G Cuts at: 2548 2708 3208 4172 5184 2548 Size: 160 500 964 1012 3476 Fragments arranged by size: 3476 1012 964 500 160 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6112 BstBI TT'CG_AA Cuts at: 4916 4916 Size: 6112 BstEII G'GTnAC_C Cuts at: 1184 2056 2176 2782 1184 Size: 872 120 606 4514 Fragments arranged by size: 4514 872 606 120 BstXI CCAn_nnnn'nTGG Cuts at: 2587 2695 3185 2587 Size: 108 490 5514 Fragments arranged by size: 5514 490 108 BstYI r'GATC_y Cuts at: 1972 3442 3809 4194 4211 5663 5749 5761 6107 Size: 1470 367 385 17 1452 86 12 346 Cuts at: 6107 1972 Size: 1977 Fragments arranged by size: 1977 1470 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2757 3141 3381 513 Size: 552 939 753 384 240 3244 Fragments arranged by size: 3244 939 753 552 384 240 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2305 2548 487 Size: 683 56 1079 243 4051 Fragments arranged by size: 4051 1079 683 243 56 BtsI GCAGTG_nn' Cuts at: 3628 4422 4442 3628 Size: 794 20 5298 Fragments arranged by size: 5298 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6112 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2410 2445 529 Size: 35 1846 35 4196 Fragments arranged by size: 4196 1846 35 35 DraI TTT'AAA Cuts at: 3416 3416 Size: 6112 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5119 809 Size: 507 3803 1802 Fragments arranged by size: 3803 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2154 2308 3033 Size: 54 1425 50 275 18 324 154 725 Cuts at: 3033 3138 3559 4448 6077 8 Size: 105 421 889 1629 43 Fragments arranged by size: 1629 1425 889 725 421 324 275 154 105 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3559 1537 Size: 275 1747 4090 Fragments arranged by size: 4090 1747 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2374 2805 3377 4042 1353 Size: 1021 431 572 665 3423 Fragments arranged by size: 3423 1021 665 572 431 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2584 4699 5073 5219 387 Size: 1064 138 995 2115 374 146 1280 Fragments arranged by size: 2115 1280 1064 995 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2355 2651 2711 4175 4772 5559 5827 Size: 393 723 296 60 1464 597 787 268 Cuts at: 5827 1239 Size: 1524 Fragments arranged by size: 1524 1464 787 723 597 393 296 268 60 EcoNI CCTnn'n_nnAGG Cuts at: 3170 3170 Size: 6112 EcoO109I rG'GnC_Cy Cuts at: 2166 2825 3097 3114 2166 Size: 659 272 17 5164 Fragments arranged by size: 5164 659 272 17 EcoRI G'AATT_C Cuts at: 3421 3799 3421 Size: 378 5734 EcoRV GAT'ATC Cuts at: 2520 2520 Size: 6112 FspI TGC'GCA Cuts at: 4618 4618 Size: 6112 HaeII r_GCGC'y Cuts at: 1678 3288 3338 5259 1678 Size: 1610 50 1921 2531 Fragments arranged by size: 2531 1921 1610 50 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2351 2383 4753 4785 4827 Size: 32 135 32 779 32 2370 32 42 Cuts at: 4827 4859 1373 Size: 32 2626 Fragments arranged by size: 2626 2370 779 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2735 2935 3358 3456 3702 669 Size: 988 281 797 200 423 98 246 3079 Fragments arranged by size: 3079 988 797 423 281 246 200 98 HindIII A'AGCT_T Cuts at: 748 748 Size: 6112 HpaI GTT'AAC Cuts at: 3702 3702 Size: 6112 KpnI G_GTAC'C Cuts at: 2122 3437 2122 Size: 1315 4797 MfeI C'AATT_G Cuts at: 3711 3711 Size: 6112 MluI A'CGCG_T Cuts at: 5988 5988 Size: 6112 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5226 5410 1305 Size: 3921 184 2007 Fragments arranged by size: 3921 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2156 3035 3140 10 Size: 54 1425 343 324 879 105 2982 Fragments arranged by size: 2982 1425 879 343 324 105 54 MslI CAynn'nnrTG Cuts at: 518 1166 2693 3140 3146 3183 4070 4429 4588 Size: 648 1527 447 6 37 887 359 159 Cuts at: 4588 518 Size: 2042 Fragments arranged by size: 2042 1527 887 648 447 359 159 37 6 MspA1I CmG'CkG Cuts at: 1606 2152 2205 2448 3225 3316 3383 3528 4205 Size: 546 53 243 777 91 67 145 677 Cuts at: 4205 5353 5598 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 777 677 546 245 243 145 91 67 53 NaeI GCC'GGC Cuts at: 2399 2399 Size: 6112 NcoI C'CATG_G Cuts at: 513 1065 2004 3141 513 Size: 552 939 1137 3484 Fragments arranged by size: 3484 1137 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6112 NgoMIV G'CCGG_C Cuts at: 2397 2397 Size: 6112 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6112 NotI GC'GGCC_GC Cuts at: 3559 3559 Size: 6112 NspI r_CATG'y Cuts at: 1132 1229 2956 3470 5015 1132 Size: 97 1727 514 1545 2229 Fragments arranged by size: 2229 1727 1545 514 97 PasI CC'CwG_GG Cuts at: 1348 2479 1348 Size: 1131 4981 PciI A'CATG_T Cuts at: 5011 5011 Size: 6112 PflMI CCAn_nnn'nTGG Cuts at: 1108 2745 1108 Size: 1637 4475 PmeI GTTT'AAAC Cuts at: 3416 3416 Size: 6112 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4181 4213 5694 5726 4181 Size: 32 1481 32 4567 Fragments arranged by size: 4567 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3097 3114 3097 Size: 17 6095 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6112 PsiI TTA'TAA Cuts at: 3682 3682 Size: 6112 PspOMI G'GGCC_C Cuts at: 2165 2824 2165 Size: 659 5453 PstI C_TGCA'G Cuts at: 830 2833 3464 830 Size: 2003 631 3478 Fragments arranged by size: 3478 2003 631 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6112 PvuII CAG'CTG Cuts at: 2152 2205 3316 2152 Size: 53 1111 4948 Fragments arranged by size: 4948 1111 53 SacII CC_GC'GG Cuts at: 3384 3384 Size: 6112 SalI G'TCGA_C Cuts at: 1936 3454 1936 Size: 1518 4594 SapI GCTCTTCn'nnn_ Cuts at: 3377 3377 Size: 6112 SbfI CC_TGCA'GG Cuts at: 2833 3464 2833 Size: 631 5481 ScaI AGT'ACT Cuts at: 4360 6072 4360 Size: 1712 4400 SexAI A'CCwGG_T Cuts at: 2778 2778 Size: 6112 SfcI C'TryA_G Cuts at: 826 1045 2829 3460 4595 5276 5467 826 Size: 219 1784 631 1135 681 191 1471 Fragments arranged by size: 1784 1471 1135 681 631 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6112 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6112 SmaI CCC'GGG Cuts at: 1872 3439 1872 Size: 1567 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4216 5117 5379 820 Size: 197 926 2273 901 262 1553 Fragments arranged by size: 2273 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3903 493 Size: 3410 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6112 SphI G_CATG'C Cuts at: 1132 2956 3470 1132 Size: 1824 514 3774 Fragments arranged by size: 3774 1824 514 SspI AAT'ATT Cuts at: 5 52 4036 5 Size: 47 3984 2081 Fragments arranged by size: 3984 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6112 StyI C'CwwG_G Cuts at: 513 1065 2004 3141 3551 513 Size: 552 939 1137 410 3074 Fragments arranged by size: 3074 1137 939 552 410 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2044 2287 2423 3202 4139 4156 4309 Size: 1255 464 243 136 779 937 17 153 Cuts at: 4309 4494 4939 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 937 779 464 445 243 185 153 136 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4358 6070 96 Size: 274 80 33 51 1443 2381 1712 138 Fragments arranged by size: 2381 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6072 6104 6072 Size: 32 6080 Tth111I GACn'n_nGTC Cuts at: 1316 2780 1316 Size: 1464 4648 XbaI T'CTAG_A Cuts at: 2866 3448 2866 Size: 582 5530 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2428 2428 Size: 6112 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6112 XmaI C'CCGG_G Cuts at: 1870 3437 1870 Size: 1567 4545 XmnI GAAnn'nnTTC Cuts at: 4241 4241 Size: 6112 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3920 276 Size: 53 83 186 936 2386 2468 Fragments arranged by size: 2468 2386 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SanDI SfiI SfoI SrfI SwaI