(Linear) (Six-base) MAPSORT of: KIEE6460.seq Check: 4629 from: 1 to: 1407 KIEE6460.orf With 182 enzymes: SgfI * March 16, 2015 11:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 26 262 1407 Size: 26 236 1145 Fragments arranged by size: 1145 236 26 Acc65I G'GTAC_C Cuts at: 0 113 1407 Size: 113 1294 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 350 1407 Size: 350 1057 AfeI AGC'GCT Cuts at: 0 1331 1407 Size: 1331 76 AleI CACnn'nnGTG Cuts at: 0 1178 1407 Size: 1178 229 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 897 1407 Size: 897 510 AlwNI CAG_nnn'CTG Cuts at: 0 740 1223 1407 Size: 740 483 184 Fragments arranged by size: 740 483 184 ApaI G_GGCC'C Cuts at: 0 164 823 1407 Size: 164 659 584 Fragments arranged by size: 659 584 164 AvaI C'yCGr_G Cuts at: 0 103 1407 Size: 103 1304 BanI G'GyrC_C Cuts at: 0 113 1407 Size: 113 1294 BanII G_rGCy'C Cuts at: 0 46 68 164 481 823 1153 1318 1407 Size: 46 22 96 317 342 330 165 89 Fragments arranged by size: 342 330 317 165 96 89 46 22 BbsI GAAGACnn'nnnn_ Cuts at: 0 24 657 764 1407 Size: 24 633 107 643 Fragments arranged by size: 643 633 107 24 BbvCI CC'TCA_GC Cuts at: 0 1018 1228 1270 1407 Size: 1018 210 42 137 Fragments arranged by size: 1018 210 137 42 BglI GCCn_nnn'nGGC Cuts at: 0 463 827 1407 Size: 463 364 580 Fragments arranged by size: 580 463 364 Bme1580I G_kGCm'C Cuts at: 0 164 218 823 1407 Size: 164 54 605 584 Fragments arranged by size: 605 584 164 54 BmrI ACTGGGnnnn_n' Cuts at: 0 1185 1407 Size: 1185 222 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1351 1407 Size: 1351 56 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 646 706 1407 Size: 646 60 701 Fragments arranged by size: 701 646 60 Bpu10I CC'TnA_GC Cuts at: 0 1018 1228 1270 1407 Size: 1018 210 42 137 Fragments arranged by size: 1018 210 137 42 BsaI GGTCTCn'nnnn_ Cuts at: 0 490 1056 1079 1407 Size: 490 566 23 328 Fragments arranged by size: 566 490 328 23 BsaWI w'CCGG_w Cuts at: 0 481 1407 Size: 481 926 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 106 136 1407 Size: 106 30 1271 Fragments arranged by size: 1271 106 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 664 754 880 1407 Size: 664 90 126 527 Fragments arranged by size: 664 527 126 90 BseYI C'CCAG_C Cuts at: 0 196 234 740 1248 1311 1407 Size: 196 38 506 508 63 96 Fragments arranged by size: 508 506 196 96 63 38 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 342 982 1327 1378 1407 Size: 342 640 345 51 29 Fragments arranged by size: 640 345 342 51 29 Bsp1286I G_dGCh'C Cuts at: 0 46 68 164 218 481 823 1153 1318 Size: 46 22 96 54 263 342 330 165 Cuts at: 1318 1407 Size: 89 Fragments arranged by size: 342 330 263 165 96 89 54 46 22 BspHI T'CATG_A Cuts at: 0 386 686 1407 Size: 386 300 721 Fragments arranged by size: 721 386 300 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 26 125 262 502 1120 1407 Size: 26 99 137 240 618 287 Fragments arranged by size: 618 287 240 137 99 26 BsrFI r'CCGG_y Cuts at: 0 392 1407 Size: 392 1015 BssSI C'ACGA_G Cuts at: 0 543 703 1203 1407 Size: 543 160 500 204 Fragments arranged by size: 543 500 204 160 BstEII G'GTnAC_C Cuts at: 0 51 171 777 1407 Size: 51 120 606 630 Fragments arranged by size: 630 606 120 51 BstXI CCAn_nnnn'nTGG Cuts at: 0 582 690 1180 1407 Size: 582 108 490 227 Fragments arranged by size: 582 490 227 108 BtgI C'CryG_G Cuts at: 0 752 1136 1376 1407 Size: 752 384 240 31 Fragments arranged by size: 752 384 240 31 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 300 543 1407 Size: 300 243 864 Fragments arranged by size: 864 300 243 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 405 440 1407 Size: 405 35 967 Fragments arranged by size: 967 405 35 EaeI y'GGCC_r Cuts at: 0 149 303 1028 1133 1407 Size: 149 154 725 105 274 Fragments arranged by size: 725 274 154 149 105 EarI CTCTTCn'nnn_ Cuts at: 0 369 800 1372 1407 Size: 369 431 572 35 Fragments arranged by size: 572 431 369 35 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 579 1407 Size: 579 828 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 350 646 706 1407 Size: 350 296 60 701 Fragments arranged by size: 701 350 296 60 EcoNI CCTnn'n_nnAGG Cuts at: 0 1165 1407 Size: 1165 242 EcoO109I rG'GnC_Cy Cuts at: 0 161 820 1092 1109 1407 Size: 161 659 272 17 298 Fragments arranged by size: 659 298 272 161 17 EcoRV GAT'ATC Cuts at: 0 515 1407 Size: 515 892 HaeII r_GCGC'y Cuts at: 0 1283 1333 1407 Size: 1283 50 74 Fragments arranged by size: 1283 74 50 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 346 378 1407 Size: 346 32 1029 Fragments arranged by size: 1029 346 32 HincII GTy'rAC Cuts at: 0 730 930 1353 1407 Size: 730 200 423 54 Fragments arranged by size: 730 423 200 54 KpnI G_GTAC'C Cuts at: 0 117 1407 Size: 117 1290 MscI TGG'CCA Cuts at: 0 151 1030 1135 1407 Size: 151 879 105 272 Fragments arranged by size: 879 272 151 105 MslI CAynn'nnrTG Cuts at: 0 688 1135 1141 1178 1407 Size: 688 447 6 37 229 Fragments arranged by size: 688 447 229 37 6 MspA1I CmG'CkG Cuts at: 0 147 200 443 1220 1311 1378 1407 Size: 147 53 243 777 91 67 29 Fragments arranged by size: 777 243 147 91 67 53 29 NaeI GCC'GGC Cuts at: 0 394 1407 Size: 394 1013 NcoI C'CATG_G Cuts at: 0 1136 1407 Size: 1136 271 NgoMIV G'CCGG_C Cuts at: 0 392 1407 Size: 392 1015 NspI r_CATG'y Cuts at: 0 951 1407 Size: 951 456 PasI CC'CwG_GG Cuts at: 0 474 1407 Size: 474 933 PflMI CCAn_nnn'nTGG Cuts at: 0 740 1407 Size: 740 667 PpuMI rG'GwC_Cy Cuts at: 0 1092 1109 1407 Size: 1092 17 298 Fragments arranged by size: 1092 298 17 PspOMI G'GGCC_C Cuts at: 0 160 819 1407 Size: 160 659 588 Fragments arranged by size: 659 588 160 PstI C_TGCA'G Cuts at: 0 828 1407 Size: 828 579 PvuII CAG'CTG Cuts at: 0 147 200 1311 1407 Size: 147 53 1111 96 Fragments arranged by size: 1111 147 96 53 SacII CC_GC'GG Cuts at: 0 1379 1407 Size: 1379 28 SapI GCTCTTCn'nnn_ Cuts at: 0 1372 1407 Size: 1372 35 SbfI CC_TGCA'GG Cuts at: 0 828 1407 Size: 828 579 SexAI A'CCwGG_T Cuts at: 0 773 1407 Size: 773 634 SfcI C'TryA_G Cuts at: 0 824 1407 Size: 824 583 SphI G_CATG'C Cuts at: 0 951 1407 Size: 951 456 StyI C'CwwG_G Cuts at: 0 1136 1407 Size: 1136 271 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 39 282 418 1197 1407 Size: 39 243 136 779 210 Fragments arranged by size: 779 243 210 136 39 Tth111I GACn'n_nGTC Cuts at: 0 775 1407 Size: 775 632 XbaI T'CTAG_A Cuts at: 0 861 1407 Size: 861 546 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 423 1407 Size: 423 984 Enzymes that do cut: AarI Acc65I AcuI AfeI AleI AlfI AlwNI ApaI AvaI BanI BanII BbsI BbvCI BglI Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaWI BsaXI BseRI BseYI BsgI Bsp1286I BspHI BspMI BsrFI BssSI BstEII BstXI BtgI BtgZI CspCI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I HincII KpnI MscI MslI MspA1I NaeI NcoI NgoMIV NspI PasI PflMI PpuMI PspOMI PstI PvuII SacII SapI SbfI SexAI SfcI SphI StyI TaqII Tth111I XbaI XcmI Enzymes that do not cut: AatII AccI AclI AflII AflIII AgeI AhdI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI BpuEI BsaAI BsaBI BsaHI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BsrBI BsrDI BsrGI BssHII BstAPI BstBI BstYI BstZ17I Bsu36I BtsI ClaI DraI DraIII DrdI EagI EcoICRI EcoRI FalI FseI FspI FspAI HindIII HpaI KasI MfeI MluI MmeI NarI NdeI NheI NotI NruI NsiI PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI StuI SwaI TatI TstI XhoI XmaI XmnI ZraI