################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BbrPI BbsI BbvCI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaI BsaWI BseAI BsiHKAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstF5I BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI Eam1105I EarI Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HincII HindII HindIII HinfI HpaI HphI KpnI Ksp632I KspI MaeI MamI MfeI MflI MluNI MroI MscI MseI MslI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 24 enzymes) # ################################### AvaII 174 GGACC G^G[AT]CC BcgI 737 GCAAGGCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BpmI 190 CTGGAG CTGGAG(16/14) BseRI 235 GAGGAG GAGGAG(10/8) BsgI 48 GTGCAG GTGCAG(16/14) BsmBI 398 GAGACG CGTCTC(1/5) BsrBI 753 CCGCTC CCGCTC(-3/-3) DdeI 508 CTGAG C^T[ACGT]AG DpnII 27 GATC ^GATC DraII 114 GGGGCCT [GA]G^G[ACGT]CC[CT] DsaI 320 CCATGG C^C[GA][CT]GG EcoO109I 114 GGGGCCT [GA]G^G[ACGT]CC[CT] MboI 27 GATC ^GATC MluI 464 ACGCGT A^CGCGT NcoI 320 CCATGG C^CATGG NdeII 27 GATC ^GATC PflMI 75 CCACCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 679 CTGCAG CTGCA^G PvuII 547 CAGCTG CAG^CTG Sau3AI 27 GATC ^GATC SfaNI 169 GATGC GCATC(5/9) SfcI 679 CTGCAG C^T[GA][CT]AG SmlI 835 CTCAAG C^T[CT][GA]AG Van91I 75 CCACCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AflIII 464 ACGCGT A^C[GA][CT]GT 584 ACGTGT BanII 208 GAGCCC G[GA]GC[CT]^C 390 GGGCTC BglI 874 GCCAAGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 907 GCCGGCCCGGC BsaAI 94 TACGTG [CT]AC^GT[GA] 583 TACGTG BsmAI 85 GAGAC GTCTC(1/5) 398 GAGAC BsrI 45 CCAGT ACTGG(1/-1) 813 CCAGT BstAPI 144 GCACCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 621 GCACAACCTGC EagI 203 CGGCCG C^GGCCG 881 CGGCCG EclXI 203 CGGCCG C^GGCCG 881 CGGCCG Eco52I 203 CGGCCG C^GGCCG 881 CGGCCG MaeIII 304 GTGAC ^GT[ACGT]AC 686 GTCAC MboII 24 GAAGA GAAGA(8/7) 332 TCTTC NspI 689 ACATGC [GA]CATG^[CT] 773 ACATGC SgrAI 221 CGCCGGCG C[GA]^CCGG[CT]G 702 CACCGGCG SmaI 110 CCCGGG CCC^GGG 121 CCCGGG TaqI 265 TCGA T^CGA 502 TCGA Tsp45I 304 GTGAC ^GT[GC]AC 686 GTCAC TthHB8I 265 TCGA T^CGA 502 TCGA XmaCI 110 CCCGGG C^CCGGG 121 CCCGGG XmaI 110 CCCGGG C^CCGGG 121 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 65 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AvaI BanI BbeI BbvI BcnI BmyI BsaHI BsaJI BsiEI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BsrFI BssHII BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I EaeI EcoRII EcoT14I FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII ItaI KasI MaeII MnlI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NgoMIV NlaIII NlaIV RsaI Sau96I ScrFI SfoI StuI StyI TseI TspRI