(Circular) (Six-base) MAPSORT of: pFN21AE5059.seq Check: 6654 from: 1 to: 5626 pFN21AE5059 5626 bp With 182 enzymes: SgfI * December 8, 2014 13:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2625 2625 Size: 5626 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3436 278 Size: 53 83 186 936 1900 2468 Fragments arranged by size: 2468 1900 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2969 1937 Size: 1032 4594 Acc65I G'GTAC_C Cuts at: 2947 2947 Size: 5626 AclI AA'CG_TT Cuts at: 1563 3753 4126 1563 Size: 2190 373 3063 Fragments arranged by size: 3063 2190 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3689 5073 5341 1632 Size: 2057 1384 268 1917 Fragments arranged by size: 2057 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5429 AflIII A'CryG_T Cuts at: 1203 2469 2589 4525 5502 1203 Size: 1266 120 1936 977 1327 Fragments arranged by size: 1936 1327 1266 977 120 AgeI A'CCGG_T Cuts at: 4457 4457 Size: 5626 AhdI GACnn_n'nnGTC Cuts at: 4355 4355 Size: 5626 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5626 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2078 1184 Size: 894 4732 AlwNI CAG_nnn'CTG Cuts at: 3369 4941 3369 Size: 1572 4054 ApaLI G'TGCA_C Cuts at: 3683 4839 3683 Size: 1156 4470 ApoI r'AATT_y Cuts at: 1493 2935 3161 3313 5422 1493 Size: 1442 226 152 2109 1697 Fragments arranged by size: 2109 1697 1442 226 152 AseI AT'TA_AT Cuts at: 160 1032 4180 160 Size: 872 3148 1606 Fragments arranged by size: 3148 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5626 AvaI C'yCGr_G Cuts at: 1870 1943 2115 2126 2161 2398 2951 1870 Size: 73 172 11 35 237 553 4545 Fragments arranged by size: 4545 553 237 172 73 35 11 BamHI G'GATC_C Cuts at: 2956 3323 2956 Size: 367 5259 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2062 2111 2233 2414 2460 Size: 325 217 627 275 49 122 181 46 Cuts at: 2460 2947 4402 618 Size: 487 1455 1842 Fragments arranged by size: 1842 1455 627 487 325 275 217 181 122 49 46 BanII G_rGCy'C Cuts at: 1391 2217 2399 2945 1391 Size: 826 182 546 4072 Fragments arranged by size: 4072 826 546 182 BbeI G_GCGC'C Cuts at: 2115 2237 2418 2115 Size: 122 181 5323 Fragments arranged by size: 5323 181 122 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4655 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2731 2765 3817 3851 1903 Size: 34 50 34 710 34 1052 34 3678 Fragments arranged by size: 3678 1052 710 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3518 4728 3518 Size: 1210 4416 BclI T'GATC_A Cuts at: 1548 1548 Size: 5626 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2885 2918 4237 Size: 107 122 71 631 605 1213 33 1319 Cuts at: 4237 136 Size: 1525 Fragments arranged by size: 1525 1319 1213 631 605 122 107 71 33 BglII A'GATC_T Cuts at: 5621 5621 Size: 5626 BlpI GC'TnA_GC Cuts at: 3043 3043 Size: 5626 Bme1580I G_kGCm'C Cuts at: 1790 2463 3687 4843 1790 Size: 673 1224 1156 2573 Fragments arranged by size: 2573 1224 1156 673 BmgBI CAC'GTC Cuts at: 1318 2502 2574 1318 Size: 1184 72 4370 Fragments arranged by size: 4370 1184 72 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3357 4315 442 Size: 416 53 83 399 24 1940 958 1753 Fragments arranged by size: 1940 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5626 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2216 4286 1239 Size: 977 2070 2579 Fragments arranged by size: 2579 2070 977 Bpu10I CC'TnA_GC Cuts at: 2513 2513 Size: 5626 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2825 3751 4616 4914 2825 Size: 926 865 298 3537 Fragments arranged by size: 3537 926 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4289 882 Size: 617 2790 2219 Fragments arranged by size: 2790 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2101 2590 3417 493 Size: 711 897 489 827 2702 Fragments arranged by size: 2702 897 827 711 489 BsaBI GATnn'nnATC Cuts at: 5620 5620 Size: 5626 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2112 2234 2331 2415 Size: 53 83 186 936 579 122 97 84 Cuts at: 2415 2649 3433 3815 4450 275 Size: 234 784 382 635 1451 Fragments arranged by size: 1451 936 784 635 579 382 234 186 122 97 84 83 53 BsaWI w'CCGG_w Cuts at: 4059 4457 4731 4878 5289 4059 Size: 398 274 147 411 4396 Fragments arranged by size: 4396 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2306 2336 1178 Size: 30 165 30 903 30 4468 Fragments arranged by size: 4468 903 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2255 1186 Size: 1069 4557 BseYI C'CCAG_C Cuts at: 4829 5325 4829 Size: 496 5130 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2074 1163 Size: 573 338 4715 Fragments arranged by size: 4715 573 338 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2211 2758 2889 3076 3837 4865 Size: 275 186 210 547 131 187 761 1028 Cuts at: 4865 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 547 275 210 187 186 131 BsiHKAI G_wGCw'C Cuts at: 3687 3772 4843 3687 Size: 85 1071 4470 Fragments arranged by size: 4470 1071 85 BsmI GAATG_Cn' Cuts at: 3137 3230 3137 Size: 93 5533 BsmBI CGTCTCn'nnnn_ Cuts at: 2397 2397 Size: 5626 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2217 2399 2463 2945 3687 3772 4843 Size: 399 427 182 64 482 742 85 1071 Cuts at: 4843 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 482 427 399 182 85 64 BspEI T'CCGG_A Cuts at: 5289 5289 Size: 5626 BspHI T'CATG_A Cuts at: 3515 5245 3515 Size: 1730 3896 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2625 2640 2775 2981 844 Size: 1781 15 135 206 3489 Fragments arranged by size: 3489 1781 206 135 15 BsrBI CCG'CTC Cuts at: 1394 1823 2760 3513 1394 Size: 429 937 753 3507 Fragments arranged by size: 3507 937 753 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4121 4295 65 Size: 1183 2873 174 1396 Fragments arranged by size: 2873 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2065 2227 2463 2708 2912 4270 4457 Size: 110 255 162 236 245 204 1358 187 Cuts at: 4457 1700 Size: 2869 Fragments arranged by size: 2869 1358 255 245 236 204 187 162 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5626 BssHII G'CGCG_C Cuts at: 1925 2282 2425 2434 2558 2730 2770 2772 1925 Size: 357 143 9 124 172 40 2 4779 Fragments arranged by size: 4779 357 172 143 124 40 9 2 BssSI C'ACGA_G Cuts at: 3686 4698 3686 Size: 1012 4614 BstAPI GCAn_nnn'nTGC Cuts at: 794 2155 2632 794 Size: 1361 477 3788 Fragments arranged by size: 3788 1361 477 BstBI TT'CG_AA Cuts at: 4430 4430 Size: 5626 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5626 BstYI r'GATC_y Cuts at: 1972 2956 3323 3708 3725 5177 5263 5275 5621 Size: 984 367 385 17 1452 86 12 346 Cuts at: 5621 1972 Size: 1977 Fragments arranged by size: 1977 1452 984 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2325 513 Size: 552 1260 3814 Fragments arranged by size: 3814 1260 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4887 Fragments arranged by size: 4887 683 56 BtsI GCAGTG_nn' Cuts at: 2313 2734 2860 3142 3936 3956 2313 Size: 421 126 282 794 20 3983 Fragments arranged by size: 3983 794 421 282 126 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5626 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5591 DraI TTT'AAA Cuts at: 2930 2930 Size: 5626 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4633 809 Size: 507 3317 1802 Fragments arranged by size: 3317 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2208 2886 2919 Size: 54 1425 50 275 18 378 678 33 Cuts at: 2919 3073 3962 5591 8 Size: 154 889 1629 43 Fragments arranged by size: 1629 1425 889 678 378 275 154 54 50 43 33 18 EagI C'GGCC_G Cuts at: 1537 1812 2208 2886 3073 1537 Size: 275 396 678 187 4090 Fragments arranged by size: 4090 678 396 275 187 EarI CTCTTCn'nnn_ Cuts at: 1353 3556 1353 Size: 2203 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4213 4587 4733 387 Size: 1064 138 2624 374 146 1280 Fragments arranged by size: 2624 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2216 3689 4286 5073 5341 1239 Size: 393 584 1473 597 787 268 1524 Fragments arranged by size: 1524 1473 787 597 584 393 268 EcoO109I rG'GnC_Cy Cuts at: 2120 2120 Size: 5626 EcoRI G'AATT_C Cuts at: 2935 3313 2935 Size: 378 5248 FspI TGC'GCA Cuts at: 4132 4132 Size: 5626 HaeII r_GCGC'y Cuts at: 1678 2115 2237 2356 2418 4773 1678 Size: 437 122 119 62 2355 2531 Fragments arranged by size: 2531 2355 437 122 119 62 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2306 2338 2339 2371 4267 Size: 32 135 32 734 32 1 32 1896 Cuts at: 4267 4299 4341 4373 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1896 734 135 42 32 32 32 32 32 32 1 HincII GTy'rAC Cuts at: 669 1657 1938 2970 3216 669 Size: 988 281 1032 246 3079 Fragments arranged by size: 3079 1032 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5626 HpaI GTT'AAC Cuts at: 3216 3216 Size: 5626 KasI G'GCGC_C Cuts at: 2111 2233 2414 2111 Size: 122 181 5323 Fragments arranged by size: 5323 181 122 KpnI G_GTAC'C Cuts at: 2951 2951 Size: 5626 MfeI C'AATT_G Cuts at: 3225 3225 Size: 5626 MluI A'CGCG_T Cuts at: 2469 5502 2469 Size: 3033 2593 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2787 4740 4924 1305 Size: 1482 1953 184 2007 Fragments arranged by size: 2007 1953 1482 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3804 Fragments arranged by size: 3804 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3584 3943 4102 518 Size: 648 2418 359 159 2042 Fragments arranged by size: 2418 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2058 2554 2811 3042 3719 4867 5112 1606 Size: 452 496 257 231 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 496 452 257 245 231 NaeI GCC'GGC Cuts at: 2067 2229 2914 2067 Size: 162 685 4779 Fragments arranged by size: 4779 685 162 NarI GG'CG_CC Cuts at: 2112 2234 2415 2112 Size: 122 181 5323 Fragments arranged by size: 5323 181 122 NcoI C'CATG_G Cuts at: 513 1065 2325 513 Size: 552 1260 3814 Fragments arranged by size: 3814 1260 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5626 NgoMIV G'CCGG_C Cuts at: 2065 2227 2912 2065 Size: 162 685 4779 Fragments arranged by size: 4779 685 162 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5626 NotI GC'GGCC_GC Cuts at: 3073 3073 Size: 5626 NspI r_CATG'y Cuts at: 1132 1229 2698 2782 2984 4529 1132 Size: 97 1469 84 202 1545 2229 Fragments arranged by size: 2229 1545 1469 202 97 84 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5626 PciI A'CATG_T Cuts at: 4525 4525 Size: 5626 PflMI CCAn_nnn'nTGG Cuts at: 1108 2086 1108 Size: 978 4648 PmeI GTTT'AAAC Cuts at: 2930 2930 Size: 5626 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3695 3727 5208 5240 3695 Size: 32 1481 32 4081 Fragments arranged by size: 4081 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5626 PsiI TTA'TAA Cuts at: 3196 3196 Size: 5626 PstI C_TGCA'G Cuts at: 830 2688 2978 830 Size: 1858 290 3478 Fragments arranged by size: 3478 1858 290 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5626 PvuII CAG'CTG Cuts at: 2554 2554 Size: 5626 SalI G'TCGA_C Cuts at: 1936 2968 1936 Size: 1032 4594 SbfI CC_TGCA'GG Cuts at: 2978 2978 Size: 5626 ScaI AGT'ACT Cuts at: 3874 5586 3874 Size: 1712 3914 SfcI C'TryA_G Cuts at: 826 1045 2684 2974 4109 4790 4981 826 Size: 219 1639 290 1135 681 191 1471 Fragments arranged by size: 1639 1471 1135 681 290 219 191 SfoI GGC'GCC Cuts at: 2113 2235 2416 2113 Size: 122 181 5323 Fragments arranged by size: 5323 181 122 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5626 SgrAI Cr'CCGG_yG Cuts at: 1700 2227 2708 1700 Size: 527 481 4618 Fragments arranged by size: 4618 527 481 SmaI CCC'GGG Cuts at: 1872 2117 2128 2953 1872 Size: 245 11 825 4545 Fragments arranged by size: 4545 825 245 11 SmlI C'TyrA_G Cuts at: 820 1017 1943 2840 3730 4631 4893 820 Size: 197 926 897 890 901 262 1553 Fragments arranged by size: 1553 926 901 897 890 262 197 SnaBI TAC'GTA Cuts at: 493 3417 493 Size: 2924 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5626 SphI G_CATG'C Cuts at: 1132 2984 1132 Size: 1852 3774 SspI AAT'ATT Cuts at: 5 52 3550 5 Size: 47 3498 2081 Fragments arranged by size: 3498 2081 47 StuI AGG'CCT Cuts at: 1517 2585 2663 2747 1517 Size: 1068 78 84 4396 Fragments arranged by size: 4396 1068 84 78 StyI C'CwwG_G Cuts at: 513 1065 2085 2325 2880 3065 513 Size: 552 1020 240 555 185 3074 Fragments arranged by size: 3074 1020 555 552 240 185 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2327 3653 3670 3823 4008 4453 325 Size: 1255 747 1326 17 153 185 445 1498 Fragments arranged by size: 1498 1326 1255 747 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2244 3872 5584 Size: 274 80 33 51 1443 267 1628 1712 Cuts at: 5584 96 Size: 138 Fragments arranged by size: 1712 1628 1443 274 267 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5586 5618 5586 Size: 32 5594 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5626 XbaI T'CTAG_A Cuts at: 2962 2962 Size: 5626 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5626 XmaI C'CCGG_G Cuts at: 1870 2115 2126 2951 1870 Size: 245 11 825 4545 Fragments arranged by size: 4545 825 245 11 XmnI GAAnn'nnTTC Cuts at: 3755 3755 Size: 5626 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3434 276 Size: 53 83 186 936 1900 2468 Fragments arranged by size: 2468 1900 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI