Restriction map for
pFN21AE5059
(Linear) (Six-base) MAPSORT of: KIEE5059.seq Check: 4604 from: 1 to: 921
KIEE5059.orf
With 182 enzymes: SgfI *
December 5, 2014 12:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 620 921
Size: 620 301
AflIII A'CryG_T
Cuts at: 0 464 584 921
Size: 464 120 337
Fragments arranged by size:
464 337 120
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 73 921
Size: 73 848
AvaI C'yCGr_G
Cuts at: 0 110 121 156 393 921
Size: 110 11 35 237 528
Fragments arranged by size:
528 237 110 35 11
BanI G'GyrC_C
Cuts at: 0 57 106 228 409 455 921
Size: 57 49 122 181 46 466
Fragments arranged by size:
466 181 122 57 49 46
BanII G_rGCy'C
Cuts at: 0 212 394 921
Size: 212 182 527
Fragments arranged by size:
527 212 182
BbeI G_GCGC'C
Cuts at: 0 110 232 413 921
Size: 110 122 181 508
Fragments arranged by size:
508 181 122 110
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 726 760 921
Size: 726 34 161
Fragments arranged by size:
726 161 34
BglI GCCn_nnn'nGGC
Cuts at: 0 880 913 921
Size: 880 33 8
Fragments arranged by size:
880 33 8
Bme1580I G_kGCm'C
Cuts at: 0 458 921
Size: 458 463
BmgBI CAC'GTC
Cuts at: 0 497 569 921
Size: 497 72 352
Fragments arranged by size:
497 352 72
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 921
Size: 211 710
Bpu10I CC'TnA_GC
Cuts at: 0 508 921
Size: 508 413
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 820 921
Size: 820 101
BsaAI yAC'GTr
Cuts at: 0 96 585 921
Size: 96 489 336
Fragments arranged by size:
489 336 96
BsaHI Gr'CG_yC
Cuts at: 0 107 229 326 410 644 921
Size: 107 122 97 84 234 277
Fragments arranged by size:
277 234 122 107 97 84
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 301 331 921
Size: 301 30 590
Fragments arranged by size:
590 301 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 250 921
Size: 250 671
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 69 921
Size: 69 852
BsiEI CG_ry'CG
Cuts at: 0 206 753 884 921
Size: 206 547 131 37
Fragments arranged by size:
547 206 131 37
BsmBI CGTCTCn'nnnn_
Cuts at: 0 392 921
Size: 392 529
Bsp1286I G_dGCh'C
Cuts at: 0 212 394 458 921
Size: 212 182 64 463
Fragments arranged by size:
463 212 182 64
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 620 635 770 921
Size: 620 15 135 151
Fragments arranged by size:
620 151 135 15
BsrBI CCG'CTC
Cuts at: 0 755 921
Size: 755 166
BsrFI r'CCGG_y
Cuts at: 0 60 222 458 703 907 921
Size: 60 162 236 245 204 14
Fragments arranged by size:
245 236 204 162 60 14
BssHII G'CGCG_C
Cuts at: 0 277 420 429 553 725 765 767 921
Size: 277 143 9 124 172 40 2 154
Fragments arranged by size:
277 172 154 143 124 40 9 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 150 627 921
Size: 150 477 294
Fragments arranged by size:
477 294 150
BtgI C'CryG_G
Cuts at: 0 320 921
Size: 320 601
BtsI GCAGTG_nn'
Cuts at: 0 308 729 855 921
Size: 308 421 126 66
Fragments arranged by size:
421 308 126 66
EaeI y'GGCC_r
Cuts at: 0 203 881 914 921
Size: 203 678 33 7
Fragments arranged by size:
678 203 33 7
EagI C'GGCC_G
Cuts at: 0 203 881 921
Size: 203 678 40
Fragments arranged by size:
678 203 40
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 921
Size: 211 710
EcoO109I rG'GnC_Cy
Cuts at: 0 115 921
Size: 115 806
HaeII r_GCGC'y
Cuts at: 0 110 232 351 413 921
Size: 110 122 119 62 508
Fragments arranged by size:
508 122 119 110 62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 301 333 334 366 921
Size: 301 32 1 32 555
Fragments arranged by size:
555 301 32 32 1
KasI G'GCGC_C
Cuts at: 0 106 228 409 921
Size: 106 122 181 512
Fragments arranged by size:
512 181 122 106
MluI A'CGCG_T
Cuts at: 0 464 921
Size: 464 457
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 782 921
Size: 782 139
MspA1I CmG'CkG
Cuts at: 0 53 549 806 921
Size: 53 496 257 115
Fragments arranged by size:
496 257 115 53
NaeI GCC'GGC
Cuts at: 0 62 224 909 921
Size: 62 162 685 12
Fragments arranged by size:
685 162 62 12
NarI GG'CG_CC
Cuts at: 0 107 229 410 921
Size: 107 122 181 511
Fragments arranged by size:
511 181 122 107
NcoI C'CATG_G
Cuts at: 0 320 921
Size: 320 601
NgoMIV G'CCGG_C
Cuts at: 0 60 222 907 921
Size: 60 162 685 14
Fragments arranged by size:
685 162 60 14
NspI r_CATG'y
Cuts at: 0 693 777 921
Size: 693 84 144
Fragments arranged by size:
693 144 84
PflMI CCAn_nnn'nTGG
Cuts at: 0 81 921
Size: 81 840
PstI C_TGCA'G
Cuts at: 0 683 921
Size: 683 238
PvuII CAG'CTG
Cuts at: 0 549 921
Size: 549 372
SfcI C'TryA_G
Cuts at: 0 679 921
Size: 679 242
SfoI GGC'GCC
Cuts at: 0 108 230 411 921
Size: 108 122 181 510
Fragments arranged by size:
510 181 122 108
SgrAI Cr'CCGG_yG
Cuts at: 0 222 703 921
Size: 222 481 218
Fragments arranged by size:
481 222 218
SmaI CCC'GGG
Cuts at: 0 112 123 921
Size: 112 11 798
Fragments arranged by size:
798 112 11
SmlI C'TyrA_G
Cuts at: 0 835 921
Size: 835 86
StuI AGG'CCT
Cuts at: 0 580 658 742 921
Size: 580 78 84 179
Fragments arranged by size:
580 179 84 78
StyI C'CwwG_G
Cuts at: 0 80 320 875 921
Size: 80 240 555 46
Fragments arranged by size:
555 240 80 46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 322 921
Size: 322 599
TatI w'GTAC_w
Cuts at: 0 239 921
Size: 239 682
XmaI C'CCGG_G
Cuts at: 0 110 121 921
Size: 110 11 800
Fragments arranged by size:
800 110 11
Enzymes that do cut:
AarI AflIII AlfI AvaI BanI BanII BbeI BcgI
BglI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaAI BsaHI
BsaXI BseRI BsgI BsiEI BsmBI Bsp1286I BspMI BsrBI
BsrFI BssHII BstAPI BtgI BtsI EaeI EagI Eco57MI
EcoO109I HaeII Hin4I KasI MluI MmeI MspA1I NaeI
NarI NcoI NgoMIV NspI PflMI PstI PvuII SfcI
SfoI SgrAI SmaI SmlI StuI StyI TaqII TatI
XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BciVI
BclI BfrBI BglII BlpI BmrI BmtI BplI BsaI
BsaBI BsaWI BseYI BsiHKAI BsiWI BsmI BspEI BspHI
BsrDI BsrGI BssSI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EarI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MscI MslI
NdeI NheI NotI NruI NsiI PacI PasI PciI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SgfI SnaBI SpeI
SphI SrfI SspI SwaI TstI Tth111I XbaI XcmI
XhoI XmnI ZraI