Restriction map for
pFN21AE3510
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
Acc65I AccI AclI AcsI AfaI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BalI BanI BbeI BbrPI
BbsI BciVI BcnI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FauI FseI HaeII HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MfeI MluI
MluNI MscI MunI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsaI RsrII
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 304 GACGTC GACGT^C
AccIII 8 TCCGGA T^CCGGA
AcyI 304 GACGTC G[GA]^CG[CT]C
AlwNI 112 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1178 GGGCCC GGGCC^C
AspHI 604 GAGCTC G[AT]GC[AT]^C
AvaI 923 CTCGGG C^[CT]CG[GA]G
AviII 15 TGCGCA TGC^GCA
BamHI 534 GGATCC G^GATCC
BcgI 1026 GCAGCGGTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 872 TGATCA T^GATCA
BpmI 364 CTCCAG CTGGAG(16/14)
BsaHI 304 GACGTC G[GA]^CG[CT]C
BsaI 887 GAGACC GGTCTC(1/5)
BsaWI 8 TCCGGA [AT]^CCGG[AT]
BseAI 8 TCCGGA T^CCGGA
BsiHKAI 604 GAGCTC G[AT]GC[AT]^C
BsoBI 923 CTCGGG C^[CT]CG[GA]G
BspEI 8 TCCGGA T^CCGGA
BsrBI 1139 GAGCGG CCGCTC(-3/-3)
BsrDI 317 CATTGC GCAATG(2/0)
BstAPI 699 GCACGTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 131 CCACTGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 1178 GGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 700 CACGTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 442 GACATTGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 39 CGGCCA [CT]^GGCC[GA]
EcoNI 247 CCTATATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1178 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 1084 CCAAGG C^C[AT][AT]GG
FbaI 872 TGATCA T^GATCA
FspI 15 TGCGCA TGC^GCA
HgaI 152 GACGC GACGC(5/10)
Hin1I 304 GACGTC G[GA]^CG[CT]C
HinfI 597 GAATC G^A[ACGT]TC
MroI 8 TCCGGA T^CCGGA
MslI 132 CACTGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NruI 780 TCGCGA TCG^CGA
PstI 1195 CTGCAG CTGCA^G
SacI 604 GAGCTC GAGCT^C
SfcI 1195 CTGCAG C^T[GA][CT]AG
SphI 338 GCATGC GCATG^C
StuI 1006 AGGCCT AGG^CCT
StyI 1084 CCAAGG C^C[AT][AT]GG
TaqI 1059 TCGA T^CGA
TfiI 597 GAATC G^A[AT]TC
TthHB8I 1059 TCGA T^CGA
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# TWO CUT ENZYMES ( 21 enzymes) #
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AccII 57 CGCG CG^CG
781 CGCG
AocI 519 CCTCAGG CC^T[ACGT]AGG
847 CCTCAGG
BsaBI 378 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
576 GATGCCCATC
BseRI 217 CTCCTC GAGGAG(10/8)
391 CTCCTC
BsgI 418 CTGCAC GTGCAG(16/14)
1145 CTGCAC
BsmAI 865 GAGAC GTCTC(1/5)
887 GAGAC
BsrI 44 ACTGG ACTGG(1/-1)
1149 ACTGG
BstUI 57 CGCG CG^CG
781 CGCG
BstYI 534 GGATCC [GA]^GATC[CT]
842 AGATCC
Bsu36I 519 CCTCAGG CC^T[ACGT]AGG
847 CCTCAGG
Eco81I 519 CCTCAGG CC^T[ACGT]AGG
847 CCTCAGG
MaeII 305 ACGT A^CGT
701 ACGT
MamI 378 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
576 GATGCCCATC
MflI 534 GGATCC [GA]^GATC[CT]
842 AGATCC
MseI 74 TTAA T^TAA
565 TTAA
MvnI 57 CGCG CG^CG
781 CGCG
NspI 338 GCATGC [GA]CATG^[CT]
678 GCATGT
SexAI 590 ACCAGGT A^CC[AT]GGT
667 ACCTGGT
Tru9I 74 TTAA T^TAA
565 TTAA
Tsp509I 503 AATT ^AATT
1106 AATT
XhoII 534 GGATCC [GA]^GATC[CT]
842 AGATCC
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# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AluI AlwI AvaII BanII
BbvCI BbvI BmyI BsaJI BsiYI
BslI BsmFI Bsp1286I BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MaeIII MboI MboII MnlI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV PvuII Sau3AI Sau96I
ScrFI SfaNI TseI Tsp45I TspRI