Restriction map for
pFN21AE3224
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# NO CUT ENZYMES ( 113 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AhdI Aor51HI
ApoI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BbrPI BbvCI BciVI BclI BfrI
BlnI BlpI Bpu1102I BsaAI BsaBI
BseAI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
Bst1107I BstBI BstZ17I CelII ClaI
CpoI DraI DraIII EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI KpnI Ksp632I
MamI MfeI MluI MluNI MroI
MscI MseI MunI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI SapI ScaI SfuI
SnaBI SpeI SphI SspBI SspI
SwaI TaqI Tru9I Tsp509I Tth111I
TthHB8I XhoI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AgeI 579 ACCGGT A^CCGGT
Alw44I 603 GTGCAC G^TGCAC
ApaLI 603 GTGCAC G^TGCAC
AscI 654 GGCGCGCC GG^CGCGCC
BamHI 1385 GGATCC G^GATCC
BbeI 867 GGCGCC GGCGC^C
BbsI 9 GAAGAC GAAGAC(2/6)
BfaI 1060 CTAG C^TAG
BpuAI 9 GAAGAC GAAGAC(2/6)
BsaWI 579 ACCGGT [AT]^CCGG[AT]
BsmBI 245 CGTCTC CGTCTC(1/5)
BsrBI 436 GAGCGG CCGCTC(-3/-3)
BsrFI 579 ACCGGT [GA]^CCGG[CT]
BstEII 1422 GGTCACC G^GT[ACGT]ACC
BstPI 1422 GGTCACC G^GT[ACGT]ACC
BstXI 92 CCATCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 579 ACCGGT [GA]^CCGG[CT]
DrdI 237 GACAGTGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 908 CCTTCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1422 GGTCACC G^GT[ACGT]ACC
HincII 1189 GTCAAC GT[CT]^[GA]AC
HindII 1189 GTCAAC GT[CT]^[GA]AC
HinfI 1497 GATTC G^A[ACGT]TC
KasI 867 GGCGCC G^GCGCC
KspI 490 CCGCGG CCGC^GG
MaeI 1060 CTAG C^TAG
MslI 343 CACTCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 867 GGCGCC GG^CGCC
NcoI 348 CCATGG C^CATGG
PflMI 1350 CCAGATCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 579 ACCGGT A^CCGGT
SacII 490 CCGCGG CCGC^GG
SbfI 924 CCTGCAGG CCTGCA^GG
SexAI 671 ACCAGGT A^CC[AT]GGT
SfiI 1444 GGCCCTGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 867 GGCGCC GGC^GCC
SgrAI 578 CACCGGTG C[GA]^CCGG[CT]G
SmlI 250 CTCAAG C^T[CT][GA]AG
Sse8387I 924 CCTGCAGG CCTGCA^GG
StuI 665 AGGCCT AGG^CCT
TfiI 1497 GATTC G^A[AT]TC
Van91I 1350 CCAGATCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1059 TCTAGA T^CTAGA
XcmI 577 CCACCGGTGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AcyI 243 GGCGTC G[GA]^CG[CT]C
867 GGCGCC
AfaI 747 GTAC GT^AC
1360 GTAC
AlwI 1386 GATCC GGATC(4/5)
1385 GGATC
AspHI 603 GTGCAC G[AT]GC[AT]^C
993 GTGCTC
BcgI 1272 CGATGTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1292 CGACAGAGATGC
BglII 459 AGATCT A^GATCT
1412 AGATCT
BpmI 502 CTGGAG CTGGAG(16/14)
1009 CTGGAG
BsaHI 243 GGCGTC G[GA]^CG[CT]C
867 GGCGCC
BsiHKAI 603 GTGCAC G[AT]GC[AT]^C
993 GTGCTC
BspMI 785 ACCTGC ACCTGC(4/8)
1168 ACCTGC
BssHII 655 GCGCGC G^CGCGC
712 GCGCGC
BssSI 323 CACGAG CACGAG(-5/-1)
990 CTCGTG
EcoT14I 348 CCATGG C^C[AT][AT]GG
1427 CCAAGG
HgaI 244 GCGTC GACGC(5/10)
741 GCGTC
Hin1I 243 GGCGTC G[GA]^CG[CT]C
867 GGCGCC
MaeII 794 ACGT A^CGT
1187 ACGT
MaeIII 205 GTCAC ^GT[ACGT]AC
1423 GTCAC
PvuII 442 CAGCTG CAG^CTG
799 CAGCTG
RsaI 747 GTAC GT^AC
1360 GTAC
SmaI 523 CCCGGG CCC^GGG
598 CCCGGG
StyI 348 CCATGG C^C[AT][AT]GG
1427 CCAAGG
Tsp45I 205 GTCAC ^GT[GC]AC
1423 GTCAC
XmaCI 523 CCCGGG C^CCGGG
598 CCCGGG
XmaI 523 CCCGGG C^CCGGG
598 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AccII AciI AluI AlwNI AocI
ApaI AvaI AvaII BanI BanII
BbvI BcnI BglI BmyI BsaI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
Eco81I EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MboI
MboII MflI MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PpuMI PstI Sau3AI
Sau96I ScrFI SfaNI SfcI TseI
TspRI XhoII