(Circular) (Six-base) MAPSORT of: pFN21AE3224.seq Check: 9785 from: 1 to: 6235 pFN21AE3224 6235 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3182 3182 Size: 6235 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4045 278 Size: 53 83 186 936 2509 2468 Fragments arranged by size: 2509 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3578 1937 Size: 1641 4594 Acc65I G'GTAC_C Cuts at: 3556 3556 Size: 6235 AclI AA'CG_TT Cuts at: 1563 4362 4735 1563 Size: 2799 373 3063 Fragments arranged by size: 3063 2799 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2159 3088 3329 4298 5682 5950 1632 Size: 527 929 241 969 1384 268 1917 Fragments arranged by size: 1917 1384 969 929 527 268 241 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6038 AflIII A'CryG_T Cuts at: 1203 5134 6111 1203 Size: 3931 977 1327 Fragments arranged by size: 3931 1327 977 AgeI A'CCGG_T Cuts at: 2584 5066 2584 Size: 2482 3753 AhdI GACnn_n'nnGTC Cuts at: 4964 4964 Size: 6235 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6235 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2580 1184 Size: 1396 4839 AlwNI CAG_nnn'CTG Cuts at: 2323 2431 2573 3978 5550 2323 Size: 108 142 1405 1572 3008 Fragments arranged by size: 3008 1572 1405 142 108 ApaI G_GGCC'C Cuts at: 2504 3440 3452 3461 2504 Size: 936 12 9 5278 Fragments arranged by size: 5278 936 12 9 ApaLI G'TGCA_C Cuts at: 2608 4292 5448 2608 Size: 1684 1156 3395 Fragments arranged by size: 3395 1684 1156 ApoI r'AATT_y Cuts at: 1493 3544 3770 3922 6031 1493 Size: 2051 226 152 2109 1697 Fragments arranged by size: 2109 2051 1697 226 152 AscI GG'CGCG_CC Cuts at: 2660 2660 Size: 6235 AseI AT'TA_AT Cuts at: 160 1032 4789 160 Size: 872 3757 1606 Fragments arranged by size: 3757 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6235 AvaI C'yCGr_G Cuts at: 1870 1943 2052 2528 2603 2666 3001 3560 1870 Size: 73 109 476 75 63 335 559 4545 Fragments arranged by size: 4545 559 476 335 109 75 73 63 BamHI G'GATC_C Cuts at: 3390 3565 3932 3390 Size: 175 367 5693 Fragments arranged by size: 5693 367 175 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2119 2872 2936 3556 5011 Size: 325 217 627 332 753 64 620 1455 Cuts at: 5011 618 Size: 1842 Fragments arranged by size: 1842 1455 753 627 620 332 325 217 64 BanII G_rGCy'C Cuts at: 1391 2504 2580 2841 3271 3440 3452 3461 3554 Size: 1113 76 261 430 169 12 9 93 Cuts at: 3554 1391 Size: 4072 Fragments arranged by size: 4072 1113 430 261 169 93 76 12 9 BbeI G_GCGC'C Cuts at: 2876 2876 Size: 6235 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2021 928 Size: 971 122 5142 Fragments arranged by size: 5142 971 122 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3266 3286 3300 3320 4426 4460 1903 Size: 34 1329 20 14 20 1106 34 3678 Fragments arranged by size: 3678 1329 1106 34 34 20 20 14 BciVI GTATCCnnnnn_n' Cuts at: 4127 5337 4127 Size: 1210 5025 BclI T'GATC_A Cuts at: 1548 1548 Size: 6235 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2632 2727 2934 Size: 107 122 71 631 605 960 95 207 Cuts at: 2934 3266 3456 4846 136 Size: 332 190 1390 1525 Fragments arranged by size: 1525 1390 960 631 605 332 207 190 122 107 95 71 BglII A'GATC_T Cuts at: 2464 3417 6230 2464 Size: 953 2813 2469 Fragments arranged by size: 2813 2469 953 BlpI GC'TnA_GC Cuts at: 3652 3652 Size: 6235 Bme1580I G_kGCm'C Cuts at: 1790 2124 2504 2603 2612 2852 2939 3440 3452 Size: 334 380 99 9 240 87 501 12 Cuts at: 3452 3461 4296 5452 1790 Size: 9 835 1156 2573 Fragments arranged by size: 2573 1156 835 501 380 334 240 99 87 12 9 9 BmgBI CAC'GTC Cuts at: 1318 2800 3193 1318 Size: 1482 393 4360 Fragments arranged by size: 4360 1482 393 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3343 3966 4924 Size: 416 53 83 399 24 1926 623 958 Cuts at: 4924 442 Size: 1753 Fragments arranged by size: 1926 1753 958 623 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6235 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2528 3035 4895 1239 Size: 1289 507 1860 2579 Fragments arranged by size: 2579 1860 1289 507 Bpu10I CC'TnA_GC Cuts at: 2042 2574 2042 Size: 532 5703 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2240 4360 5225 5523 2240 Size: 2120 865 298 2952 Fragments arranged by size: 2952 2120 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2155 2325 2333 2971 4898 882 Size: 617 656 170 8 638 1927 2219 Fragments arranged by size: 2219 1927 656 638 617 170 8 BsaAI yAC'GTr Cuts at: 493 1204 4026 493 Size: 711 2822 2702 Fragments arranged by size: 2822 2702 711 BsaBI GATnn'nnATC Cuts at: 6229 6229 Size: 6235 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2249 2873 4042 4424 Size: 53 83 186 936 716 624 1169 382 Cuts at: 4424 5059 275 Size: 635 1451 Fragments arranged by size: 1451 1169 936 716 635 624 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2584 4668 5066 5340 5487 5898 2584 Size: 2084 398 274 147 411 2921 Fragments arranged by size: 2921 2084 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6010 Fragments arranged by size: 6010 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2720 2723 2747 2750 2830 1186 Size: 1534 3 24 3 80 4591 Fragments arranged by size: 4591 1534 80 24 3 3 BseYI C'CCAG_C Cuts at: 3423 3444 5438 5934 3423 Size: 21 1994 496 3724 Fragments arranged by size: 3724 1994 496 21 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2330 2891 3382 1163 Size: 573 594 561 491 4016 Fragments arranged by size: 4016 594 573 561 491 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3685 4446 5474 1540 Size: 275 186 1684 761 1028 2301 Fragments arranged by size: 2301 1684 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2612 3002 4296 4381 5452 2612 Size: 390 1294 85 1071 3395 Fragments arranged by size: 3395 1294 1071 390 85 BsmI GAATG_Cn' Cuts at: 3746 3839 3746 Size: 93 6142 BsmBI CGTCTCn'nnnn_ Cuts at: 2256 2256 Size: 6235 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2124 2504 2580 2603 2612 2841 2852 Size: 399 334 380 76 23 9 229 11 Cuts at: 2852 2939 3002 3271 3440 3452 3461 3554 4296 Size: 87 63 269 169 12 9 93 742 Cuts at: 4296 4381 5452 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 380 334 269 229 169 93 87 85 76 63 23 12 11 9 9 BspEI T'CCGG_A Cuts at: 5898 5898 Size: 6235 BspHI T'CATG_A Cuts at: 4124 5854 4124 Size: 1730 4505 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2799 3182 3590 844 Size: 1955 383 408 3489 Fragments arranged by size: 3489 1955 408 383 BsrBI CCG'CTC Cuts at: 1394 1823 2443 4122 1394 Size: 429 620 1679 3507 Fragments arranged by size: 3507 1679 620 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2091 3081 3112 4730 4904 65 Size: 1183 843 990 31 1618 174 1396 Fragments arranged by size: 1618 1396 1183 990 843 174 31 BsrFI r'CCGG_y Cuts at: 1700 1810 2584 4879 5066 1700 Size: 110 774 2295 187 2869 Fragments arranged by size: 2869 2295 774 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6235 BssHII G'CGCG_C Cuts at: 1925 2660 2717 1925 Size: 735 57 5443 Fragments arranged by size: 5443 735 57 BssSI C'ACGA_G Cuts at: 2328 2995 4295 5307 2328 Size: 667 1300 1012 3256 Fragments arranged by size: 3256 1300 1012 667 BstAPI GCAn_nnn'nTGC Cuts at: 794 2119 2925 3405 794 Size: 1325 806 480 3624 Fragments arranged by size: 3624 1325 806 480 BstBI TT'CG_AA Cuts at: 5039 5039 Size: 6235 BstEII G'GTnAC_C Cuts at: 1184 3427 1184 Size: 2243 3992 BstXI CCAn_nnnn'nTGG Cuts at: 2104 2104 Size: 6235 BstYI r'GATC_y Cuts at: 1972 2464 3390 3417 3565 3932 4317 4334 5786 Size: 492 926 27 148 367 385 17 1452 Cuts at: 5786 5872 5884 6230 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 926 492 385 367 346 148 86 27 17 12 Bsu36I CC'TnA_GG Cuts at: 2145 2424 3045 2145 Size: 279 621 5335 Fragments arranged by size: 5335 621 279 BtgI C'CryG_G Cuts at: 513 1065 2004 2353 2495 2886 513 Size: 552 939 349 142 391 3862 Fragments arranged by size: 3862 939 552 391 349 142 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5496 Fragments arranged by size: 5496 683 56 BtsI GCAGTG_nn' Cuts at: 2064 2309 2574 2603 3103 3751 4545 4565 2064 Size: 245 265 29 500 648 794 20 3734 Fragments arranged by size: 3734 794 648 500 265 245 29 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6235 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2277 2312 529 Size: 35 1713 35 4452 Fragments arranged by size: 4452 1713 35 35 DraI TTT'AAA Cuts at: 3539 3539 Size: 6235 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2248 5242 809 Size: 507 932 2994 1802 Fragments arranged by size: 2994 1802 932 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3682 4571 6200 Size: 54 1425 50 275 18 1852 889 1629 Cuts at: 6200 8 Size: 43 Fragments arranged by size: 1852 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3682 1537 Size: 275 1870 4090 Fragments arranged by size: 4090 1870 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4165 1353 Size: 2812 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2975 4822 5196 5342 387 Size: 1064 138 1386 1847 374 146 1280 Fragments arranged by size: 1847 1386 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2159 2528 3035 3088 3329 4298 4895 Size: 393 527 369 507 53 241 969 597 Cuts at: 4895 5682 5950 1239 Size: 787 268 1524 Fragments arranged by size: 1524 969 787 597 527 507 393 369 268 241 53 EcoNI CCTnn'n_nnAGG Cuts at: 2917 2917 Size: 6235 EcoO109I rG'GnC_Cy Cuts at: 2479 2500 2501 2516 2533 2680 3253 3436 3448 Size: 21 1 15 17 147 573 183 12 Cuts at: 3448 3449 3457 2479 Size: 1 8 5257 Fragments arranged by size: 5257 573 183 147 21 17 15 12 8 1 1 EcoRI G'AATT_C Cuts at: 3544 3922 3544 Size: 378 5857 FspI TGC'GCA Cuts at: 4741 4741 Size: 6235 HaeII r_GCGC'y Cuts at: 1678 2744 2876 3035 5382 1678 Size: 1066 132 159 2347 2531 Fragments arranged by size: 2531 2347 1066 159 132 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4876 4908 4950 4982 1373 Size: 32 135 32 3304 32 42 32 2626 Fragments arranged by size: 3304 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3196 3579 3825 669 Size: 988 281 1258 383 246 3079 Fragments arranged by size: 3079 1258 988 383 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6235 HpaI GTT'AAC Cuts at: 3825 3825 Size: 6235 KasI G'GCGC_C Cuts at: 2872 2872 Size: 6235 KpnI G_GTAC'C Cuts at: 3560 3560 Size: 6235 MfeI C'AATT_G Cuts at: 3834 3834 Size: 6235 MluI A'CGCG_T Cuts at: 6111 6111 Size: 6235 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5349 5533 1305 Size: 4044 184 2007 Fragments arranged by size: 4044 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4413 Fragments arranged by size: 4413 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2352 4193 4552 4711 518 Size: 648 1186 1841 359 159 2042 Fragments arranged by size: 2042 1841 1186 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2320 2437 2449 2497 2761 2806 3396 3474 Size: 714 117 12 48 264 45 590 78 Cuts at: 3474 3486 3651 4328 5476 5721 1606 Size: 12 165 677 1148 245 2120 Fragments arranged by size: 2120 1148 714 677 590 264 245 165 117 78 48 45 12 12 NarI GG'CG_CC Cuts at: 2873 2873 Size: 6235 NcoI C'CATG_G Cuts at: 513 1065 2004 2353 513 Size: 552 939 349 4395 Fragments arranged by size: 4395 939 552 349 NdeI CA'TA_TG Cuts at: 387 387 Size: 6235 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6235 NotI GC'GGCC_GC Cuts at: 3682 3682 Size: 6235 NspI r_CATG'y Cuts at: 1132 1229 3593 5138 1132 Size: 97 2364 1545 2229 Fragments arranged by size: 2364 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3452 1348 Size: 2104 4131 PciI A'CATG_T Cuts at: 5134 5134 Size: 6235 PflMI CCAn_nnn'nTGG Cuts at: 1108 3361 1108 Size: 2253 3982 PmeI GTTT'AAAC Cuts at: 3539 3539 Size: 6235 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4304 4336 5817 5849 4304 Size: 32 1481 32 4690 Fragments arranged by size: 4690 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2479 2533 2680 3253 2479 Size: 54 147 573 5461 Fragments arranged by size: 5461 573 147 54 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6235 PsiI TTA'TAA Cuts at: 3805 3805 Size: 6235 PspOMI G'GGCC_C Cuts at: 2500 3436 3448 3457 2500 Size: 936 12 9 5278 Fragments arranged by size: 5278 936 12 9 PstI C_TGCA'G Cuts at: 830 2436 2493 2796 2921 2934 3324 3491 3587 Size: 1606 57 303 125 13 390 167 96 Cuts at: 3587 830 Size: 3478 Fragments arranged by size: 3478 1606 390 303 167 125 96 57 13 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6235 PvuII CAG'CTG Cuts at: 2449 2806 2449 Size: 357 5878 SacII CC_GC'GG Cuts at: 2498 2498 Size: 6235 SalI G'TCGA_C Cuts at: 1936 3577 1936 Size: 1641 4594 SanDI GG'GwC_CC Cuts at: 2533 2533 Size: 6235 SbfI CC_TGCA'GG Cuts at: 2934 3587 2934 Size: 653 5582 ScaI AGT'ACT Cuts at: 4483 6195 4483 Size: 1712 4523 SexAI A'CCwGG_T Cuts at: 2676 2676 Size: 6235 SfcI C'TryA_G Cuts at: 826 1045 2432 2489 2792 2917 2930 3320 3487 Size: 219 1387 57 303 125 13 390 167 Cuts at: 3487 3583 4718 5399 5590 826 Size: 96 1135 681 191 1471 Fragments arranged by size: 1471 1387 1135 681 390 303 219 191 167 125 96 57 13 SfiI GGCCn_nnn'nGGCC Cuts at: 3456 3456 Size: 6235 SfoI GGC'GCC Cuts at: 2874 2874 Size: 6235 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6235 SgrAI Cr'CCGG_yG Cuts at: 1700 2584 1700 Size: 884 5351 SmaI CCC'GGG Cuts at: 1872 2530 2605 3562 1872 Size: 658 75 957 4545 Fragments arranged by size: 4545 957 658 75 SmlI C'TyrA_G Cuts at: 820 1017 1943 2255 4339 5240 5502 820 Size: 197 926 312 2084 901 262 1553 Fragments arranged by size: 2084 1553 926 901 312 262 197 SnaBI TAC'GTA Cuts at: 493 4026 493 Size: 3533 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6235 SphI G_CATG'C Cuts at: 1132 3593 1132 Size: 2461 3774 SspI AAT'ATT Cuts at: 5 52 4159 5 Size: 47 4107 2081 Fragments arranged by size: 4107 2081 47 StuI AGG'CCT Cuts at: 1517 2672 1517 Size: 1155 5080 StyI C'CwwG_G Cuts at: 513 1065 2004 2353 3432 3674 513 Size: 552 939 349 1079 242 3074 Fragments arranged by size: 3074 1079 939 552 349 242 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2167 2296 3528 4262 4279 4432 4617 Size: 1255 587 129 1232 734 17 153 185 Cuts at: 4617 5062 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1232 734 587 445 185 153 129 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4481 6193 96 Size: 274 80 33 51 1443 2504 1712 138 Fragments arranged by size: 2504 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6195 6227 6195 Size: 32 6203 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6235 XbaI T'CTAG_A Cuts at: 3064 3571 3064 Size: 507 5728 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2589 2589 Size: 6235 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6235 XmaI C'CCGG_G Cuts at: 1870 2528 2603 3560 1870 Size: 658 75 957 4545 Fragments arranged by size: 4545 957 658 75 XmnI GAAnn'nnTTC Cuts at: 4364 4364 Size: 6235 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4043 276 Size: 53 83 186 936 2509 2468 Fragments arranged by size: 2509 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AvrII BaeI BbvCI BfrBI BplI BsiWI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SapI SrfI SwaI