Restriction map for
pFN21AE3224
(Linear) (Six-base) MAPSORT of: KIEE3224.seq Check: 1100 from: 1 to: 1530
KIEE3224.orf
With 182 enzymes: SgfI *
December 27, 2011 16:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1177 1530
Size: 1177 353
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 1083 1324 1530
Size: 154 929 241 206
Fragments arranged by size:
929 241 206 154
AgeI A'CCGG_T
Cuts at: 0 579 1530
Size: 579 951
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 575 1530
Size: 575 955
AlwNI CAG_nnn'CTG
Cuts at: 0 318 426 568 1530
Size: 318 108 142 962
Fragments arranged by size:
962 318 142 108
ApaI G_GGCC'C
Cuts at: 0 499 1435 1447 1456 1530
Size: 499 936 12 9 74
Fragments arranged by size:
936 499 74 12 9
ApaLI G'TGCA_C
Cuts at: 0 603 1530
Size: 603 927
AscI GG'CGCG_CC
Cuts at: 0 655 1530
Size: 655 875
AvaI C'yCGr_G
Cuts at: 0 47 523 598 661 996 1530
Size: 47 476 75 63 335 534
Fragments arranged by size:
534 476 335 75 63 47
BamHI G'GATC_C
Cuts at: 0 1385 1530
Size: 1385 145
BanI G'GyrC_C
Cuts at: 0 114 867 931 1530
Size: 114 753 64 599
Fragments arranged by size:
753 599 114 64
BanII G_rGCy'C
Cuts at: 0 499 575 836 1266 1435 1447 1456 1530
Size: 499 76 261 430 169 12 9 74
Fragments arranged by size:
499 430 261 169 76 74 12 9
BbeI G_GCGC'C
Cuts at: 0 871 1530
Size: 871 659
BbsI GAAGACnn'nnnn_
Cuts at: 0 16 1530
Size: 16 1514
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1261 1281 1295 1315 1530
Size: 1261 20 14 20 215
Fragments arranged by size:
1261 215 20 20 14
BglI GCCn_nnn'nGGC
Cuts at: 0 627 722 929 1261 1451 1530
Size: 627 95 207 332 190 79
Fragments arranged by size:
627 332 207 190 95 79
BglII A'GATC_T
Cuts at: 0 459 1412 1530
Size: 459 953 118
Fragments arranged by size:
953 459 118
Bme1580I G_kGCm'C
Cuts at: 0 119 499 598 607 847 934 1435 1447
Size: 119 380 99 9 240 87 501 12
Cuts at: 1447 1456 1530
Size: 9 74
Fragments arranged by size:
501 380 240 119 99 87 74 12
9 9
BmgBI CAC'GTC
Cuts at: 0 795 1188 1530
Size: 795 393 342
Fragments arranged by size:
795 393 342
BmrI ACTGGGnnnn_n'
Cuts at: 0 1338 1530
Size: 1338 192
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 523 1030 1530
Size: 523 507 500
Fragments arranged by size:
523 507 500
Bpu10I CC'TnA_GC
Cuts at: 0 37 569 1530
Size: 37 532 961
Fragments arranged by size:
961 532 37
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 1530
Size: 235 1295
BsaI GGTCTCn'nnnn_
Cuts at: 0 150 320 328 966 1530
Size: 150 170 8 638 564
Fragments arranged by size:
638 564 170 150 8
BsaHI Gr'CG_yC
Cuts at: 0 244 868 1530
Size: 244 624 662
Fragments arranged by size:
662 624 244
BsaWI w'CCGG_w
Cuts at: 0 579 1530
Size: 579 951
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 715 718 742 745 825 1530
Size: 715 3 24 3 80 705
Fragments arranged by size:
715 705 80 24 3 3
BseYI C'CCAG_C
Cuts at: 0 1418 1439 1530
Size: 1418 21 91
Fragments arranged by size:
1418 91 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 886 1377 1530
Size: 325 561 491 153
Fragments arranged by size:
561 491 325 153
BsiHKAI G_wGCw'C
Cuts at: 0 607 997 1530
Size: 607 390 533
Fragments arranged by size:
607 533 390
BsmBI CGTCTCn'nnnn_
Cuts at: 0 251 1530
Size: 251 1279
Bsp1286I G_dGCh'C
Cuts at: 0 119 499 575 598 607 836 847 934
Size: 119 380 76 23 9 229 11 87
Cuts at: 934 997 1266 1435 1447 1456 1530
Size: 63 269 169 12 9 74
Fragments arranged by size:
380 269 229 169 119 87 76 74
63 23 12 11 9 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 794 1177 1530
Size: 794 383 353
Fragments arranged by size:
794 383 353
BsrBI CCG'CTC
Cuts at: 0 438 1530
Size: 438 1092
BsrDI GCAATG_nn'
Cuts at: 0 86 1076 1107 1530
Size: 86 990 31 423
Fragments arranged by size:
990 423 86 31
BsrFI r'CCGG_y
Cuts at: 0 579 1530
Size: 579 951
BssHII G'CGCG_C
Cuts at: 0 655 712 1530
Size: 655 57 818
Fragments arranged by size:
818 655 57
BssSI C'ACGA_G
Cuts at: 0 323 990 1530
Size: 323 667 540
Fragments arranged by size:
667 540 323
BstAPI GCAn_nnn'nTGC
Cuts at: 0 114 920 1400 1530
Size: 114 806 480 130
Fragments arranged by size:
806 480 130 114
BstEII G'GTnAC_C
Cuts at: 0 1422 1530
Size: 1422 108
BstXI CCAn_nnnn'nTGG
Cuts at: 0 99 1530
Size: 99 1431
BstYI r'GATC_y
Cuts at: 0 459 1385 1412 1530
Size: 459 926 27 118
Fragments arranged by size:
926 459 118 27
Bsu36I CC'TnA_GG
Cuts at: 0 140 419 1040 1530
Size: 140 279 621 490
Fragments arranged by size:
621 490 279 140
BtgI C'CryG_G
Cuts at: 0 348 490 881 1530
Size: 348 142 391 649
Fragments arranged by size:
649 391 348 142
BtsI GCAGTG_nn'
Cuts at: 0 59 304 569 598 1098 1530
Size: 59 245 265 29 500 432
Fragments arranged by size:
500 432 265 245 59 29
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 272 307 1530
Size: 272 35 1223
Fragments arranged by size:
1223 272 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 243 1530
Size: 243 1287
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 970 1530
Size: 970 560
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 523 1030 1083 1324 1530
Size: 154 369 507 53 241 206
Fragments arranged by size:
507 369 241 206 154 53
EcoNI CCTnn'n_nnAGG
Cuts at: 0 912 1530
Size: 912 618
EcoO109I rG'GnC_Cy
Cuts at: 0 474 495 496 511 528 675 1248 1431
Size: 474 21 1 15 17 147 573 183
Cuts at: 1431 1443 1444 1452 1530
Size: 12 1 8 78
Fragments arranged by size:
573 474 183 147 78 21 17 15
12 8 1 1
HaeII r_GCGC'y
Cuts at: 0 739 871 1030 1530
Size: 739 132 159 500
Fragments arranged by size:
739 500 159 132
HincII GTy'rAC
Cuts at: 0 1191 1530
Size: 1191 339
KasI G'GCGC_C
Cuts at: 0 867 1530
Size: 867 663
MslI CAynn'nnrTG
Cuts at: 0 347 1530
Size: 347 1183
MspA1I CmG'CkG
Cuts at: 0 315 432 444 492 756 801 1391 1469
Size: 315 117 12 48 264 45 590 78
Cuts at: 1469 1481 1530
Size: 12 49
Fragments arranged by size:
590 315 264 117 78 49 48 45
12 12
NarI GG'CG_CC
Cuts at: 0 868 1530
Size: 868 662
NcoI C'CATG_G
Cuts at: 0 348 1530
Size: 348 1182
PasI CC'CwG_GG
Cuts at: 0 1447 1530
Size: 1447 83
PflMI CCAn_nnn'nTGG
Cuts at: 0 1356 1530
Size: 1356 174
PpuMI rG'GwC_Cy
Cuts at: 0 474 528 675 1248 1530
Size: 474 54 147 573 282
Fragments arranged by size:
573 474 282 147 54
PspOMI G'GGCC_C
Cuts at: 0 495 1431 1443 1452 1530
Size: 495 936 12 9 78
Fragments arranged by size:
936 495 78 12 9
PstI C_TGCA'G
Cuts at: 0 431 488 791 916 929 1319 1486 1530
Size: 431 57 303 125 13 390 167 44
Fragments arranged by size:
431 390 303 167 125 57 44 13
PvuII CAG'CTG
Cuts at: 0 444 801 1530
Size: 444 357 729
Fragments arranged by size:
729 444 357
SacII CC_GC'GG
Cuts at: 0 493 1530
Size: 493 1037
SanDI GG'GwC_CC
Cuts at: 0 528 1530
Size: 528 1002
SbfI CC_TGCA'GG
Cuts at: 0 929 1530
Size: 929 601
SexAI A'CCwGG_T
Cuts at: 0 671 1530
Size: 671 859
SfcI C'TryA_G
Cuts at: 0 427 484 787 912 925 1315 1482 1530
Size: 427 57 303 125 13 390 167 48
Fragments arranged by size:
427 390 303 167 125 57 48 13
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1451 1530
Size: 1451 79
SfoI GGC'GCC
Cuts at: 0 869 1530
Size: 869 661
SgrAI Cr'CCGG_yG
Cuts at: 0 579 1530
Size: 579 951
SmaI CCC'GGG
Cuts at: 0 525 600 1530
Size: 525 75 930
Fragments arranged by size:
930 525 75
SmlI C'TyrA_G
Cuts at: 0 250 1530
Size: 250 1280
StuI AGG'CCT
Cuts at: 0 667 1530
Size: 667 863
StyI C'CwwG_G
Cuts at: 0 348 1427 1530
Size: 348 1079 103
Fragments arranged by size:
1079 348 103
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 162 291 1523 1530
Size: 162 129 1232 7
Fragments arranged by size:
1232 162 129 7
XbaI T'CTAG_A
Cuts at: 0 1059 1530
Size: 1059 471
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 584 1530
Size: 584 946
XmaI C'CCGG_G
Cuts at: 0 523 598 1530
Size: 523 75 932
Fragments arranged by size:
932 523 75
Enzymes that do cut:
AarI AcuI AgeI AlfI AlwNI ApaI ApaLI AscI
AvaI BamHI BanI BanII BbeI BbsI BcgI BglI
BglII Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI
BsaHI BsaWI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtsI CspCI DrdI EciI
Eco57MI EcoNI EcoO109I HaeII HincII KasI MslI MspA1I
NarI NcoI PasI PflMI PpuMI PspOMI PstI PvuII
SacII SanDI SbfI SexAI SfcI SfiI SfoI SgrAI
SmaI SmlI StuI StyI TaqII XbaI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AhdI
AleI AloI ApoI AseI AsiSI AvrII BaeI BbvCI
BciVI BclI BfrBI BlpI BmtI BplI BsaAI BsaBI
BsaXI BsiEI BsiWI BsmI BspEI BspHI BsrGI BstBI
BstZ17I BtgZI ClaI DraI DraIII EaeI EagI EarI
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI Hin4I
HindIII HpaI KpnI MfeI MluI MmeI MscI NaeI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SalI SapI ScaI SgfI
SnaBI SpeI SphI SrfI SspI SwaI TatI TstI
Tth111I XhoI XmnI ZraI