################################## # NO CUT ENZYMES ( 122 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AviII AvrII BalI BamHI BbrPI BcgI BciVI BclI BfrI BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaWI BseAI BsiHKAI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstBI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII Eam1105I Eco47III Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FspI HincII HindII HindIII HpaI KpnI MamI MfeI MluI MluNI MroI MscI MseI MslI MunI NcoI NdeI NheI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp45I Tsp509I Van91I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AfaI 324 GTAC GT^AC Asp700I 132 GAACGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 657 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC BanII 85 GAGCCC G[GA]GC[CT]^C BbsI 499 GAAGAC GAAGAC(2/6) BbvCI 709 GCTGAGG CCTCAGC(-5/-2) BfaI 917 CTAG C^TAG BglII 206 AGATCT A^GATCT BpuAI 499 GAAGAC GAAGAC(2/6) BsaI 538 GAGACC GGTCTC(1/5) BsmBI 727 GAGACG CGTCTC(1/5) BsmI 136 GCATTC GAATGC(1/-1) BspMI 824 ACCTGC ACCTGC(4/8) BstAPI 234 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 882 GGTTACC G^GT[ACGT]ACC BstF5I 563 GGATG GGATG(2/0) BstPI 882 GGTTACC G^GT[ACGT]ACC BstXI 212 CCAAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DdeI 710 CTGAG C^T[ACGT]AG DrdI 729 GACGGTGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 432 CCGCGG C^C[GA][CT]GG EcoO65I 882 GGTTACC G^GT[ACGT]ACC FokI 563 GGATG GGATG(9/13) HgaI 736 GCGTC GACGC(5/10) KspI 432 CCGCGG CCGC^GG MaeI 917 CTAG C^TAG MaeII 844 ACGT A^CGT MaeIII 883 GTTAC ^GT[ACGT]AC NotI 631 GCGGCCGC GC^GGCCGC NruI 813 TCGCGA TCG^CGA NspI 191 ACATGC [GA]CATG^[CT] PflFI 657 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 54 GGGACCC [GA]G^G[AT]CC[CT] RsaI 324 GTAC GT^AC SacII 432 CCGCGG CCGC^GG SexAI 929 ACCTGGT A^CC[AT]GGT SfaNI 920 GCATC GCATC(5/9) TspRI 181 CAGTG CA[GC]TG[ACGT][ACGT]^ Tth111I 657 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 132 GAACGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AlwI 258 GATCC GGATC(4/5) 810 GGATC AlwNI 235 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG 704 CAGCGGCTG BglI 431 GCCGCGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 782 GCCCGGCCGGC BmyI 85 GAGCCC G[AGT]GC[ACT]^C 840 GGGCAC BsgI 870 CTGCAC GTGCAG(16/14) 4 GTGCAG BsmAI 538 GAGAC GTCTC(1/5) 727 GAGAC Bsp1286I 85 GAGCCC G[AGT]GC[ACT]^C 840 GGGCAC BsrI 905 ACTGG ACTGG(1/-1) 180 CCAGT BssHII 79 GCGCGC G^CGCGC 603 GCGCGC BstYI 206 AGATCT [GA]^GATC[CT] 257 AGATCC DraII 54 GGGACCC [GA]G^G[ACGT]CC[CT] 456 AGGGCCT EarI 685 GAAGAG CTCTTC(1/4) 721 GAAGAG EcoO109I 54 GGGACCC [GA]G^G[ACGT]CC[CT] 456 AGGGCCT EcoT14I 359 CCAAGG C^C[AT][AT]GG 609 CCAAGG FseI 62 GGCCGGCC GGCCGG^CC 786 GGCCGGCC HinfI 247 GACTC G^A[ACGT]TC 748 GAGTC HphI 718 GGTGA GGTGA(8/7) 938 TCACC Ksp632I 685 GAAGAG CTCTTC(1/4) 721 GAAGAG MflI 206 AGATCT [GA]^GATC[CT] 257 AGATCC PleI 247 GACTC GAGTC(4/5) 748 GAGTC PvuII 235 CAGCTG CAG^CTG 421 CAGCTG SfiI 430 GGCCGCGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 781 GGCCCGGCCGGCC SmlI 292 CTCAAG C^T[CT][GA]AG 406 CTCAAG StyI 359 CCAAGG C^C[AT][AT]GG 609 CCAAGG TaqI 536 TCGA T^CGA 854 TCGA TthHB8I 536 TCGA T^CGA 854 TCGA XhoII 206 AGATCT [GA]^GATC[CT] 257 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AccII AciI AcyI AluI AvaI AvaII BanI BbeI BbvI BcnI BsaHI BsaJI BseRI BsiEI BsiYI BslI BsmFI BsoBI BsrBI BsrFI BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DpnII EaeI EagI EclXI Eco52I EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII ItaI KasI MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV PstI Sau3AI Sau96I ScrFI SfcI SfoI SmaI TseI XmaCI XmaI