(Circular) (Six-base) MAPSORT of: pFN21AB9650.seq Check: 9277 from: 1 to: 5650 pFN21AB9650 5650 bp With 182 enzymes: SgfI * March 28, 2012 14:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3460 278 Size: 53 83 186 936 1924 2468 Fragments arranged by size: 2468 1924 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2993 1937 Size: 1056 4594 Acc65I G'GTAC_C Cuts at: 2971 2971 Size: 5650 AclI AA'CG_TT Cuts at: 1563 3777 4150 1563 Size: 2214 373 3063 Fragments arranged by size: 3063 2214 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3713 5097 5365 1632 Size: 2081 1384 268 1917 Fragments arranged by size: 2081 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5453 AflIII A'CryG_T Cuts at: 1203 4549 5526 1203 Size: 3346 977 1327 Fragments arranged by size: 3346 1327 977 AgeI A'CCGG_T Cuts at: 4481 4481 Size: 5650 AhdI GACnn_n'nnGTC Cuts at: 4379 4379 Size: 5650 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5650 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5650 AlwNI CAG_nnn'CTG Cuts at: 2245 2714 3393 4965 2245 Size: 469 679 1572 2930 Fragments arranged by size: 2930 1572 679 469 ApaLI G'TGCA_C Cuts at: 3707 4863 3707 Size: 1156 4494 ApoI r'AATT_y Cuts at: 1493 2959 3185 3337 5446 1493 Size: 1466 226 152 2109 1697 Fragments arranged by size: 2109 1697 1466 226 152 AseI AT'TA_AT Cuts at: 160 1032 4204 160 Size: 872 3172 1606 Fragments arranged by size: 3172 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5650 AvaI C'yCGr_G Cuts at: 1870 1943 2056 2063 2110 2417 2489 2548 2592 Size: 73 113 7 47 307 72 59 44 Cuts at: 2592 2842 2882 2975 1870 Size: 250 40 93 4545 Fragments arranged by size: 4545 307 250 113 93 73 72 59 47 44 40 7 BamHI G'GATC_C Cuts at: 2980 3347 2980 Size: 367 5283 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2356 2485 2913 2971 4426 Size: 325 217 627 569 129 428 58 1455 Cuts at: 4426 618 Size: 1842 Fragments arranged by size: 1842 1455 627 569 428 325 217 129 58 BanII G_rGCy'C Cuts at: 1391 2094 2969 1391 Size: 703 875 4072 Fragments arranged by size: 4072 875 703 BbeI G_GCGC'C Cuts at: 2360 2489 2917 2360 Size: 129 428 5093 Fragments arranged by size: 5093 428 129 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2511 928 Size: 971 612 4067 Fragments arranged by size: 4067 971 612 BbvCI CC'TCA_GC Cuts at: 2715 2715 Size: 5650 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3841 3875 1903 Size: 34 1904 34 3678 Fragments arranged by size: 3678 1904 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3542 4752 3542 Size: 1210 4440 BclI T'GATC_A Cuts at: 1548 1548 Size: 5650 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2442 2793 4261 Size: 107 122 71 631 605 770 351 1468 Cuts at: 4261 136 Size: 1525 Fragments arranged by size: 1525 1468 770 631 605 351 122 107 71 BglII A'GATC_T Cuts at: 2211 5645 2211 Size: 3434 2216 BlpI GC'TnA_GC Cuts at: 3067 3067 Size: 5650 Bme1580I G_kGCm'C Cuts at: 1790 2849 3711 4867 1790 Size: 1059 862 1156 2573 Fragments arranged by size: 2573 1156 1059 862 BmgBI CAC'GTC Cuts at: 1318 2850 1318 Size: 1532 4118 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3381 4339 442 Size: 416 53 83 399 24 1964 958 1753 Fragments arranged by size: 1964 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5650 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4310 1239 Size: 3071 2579 Bpu10I CC'TnA_GC Cuts at: 2715 2715 Size: 5650 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2282 2396 3775 4640 4938 2282 Size: 114 1379 865 298 2994 Fragments arranged by size: 2994 1379 865 298 114 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2537 4313 882 Size: 617 1038 1776 2219 Fragments arranged by size: 2219 1776 1038 617 BsaAI yAC'GTr Cuts at: 493 1204 3441 493 Size: 711 2237 2702 Fragments arranged by size: 2702 2237 711 BsaBI GATnn'nnATC Cuts at: 5644 5644 Size: 5650 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2357 2486 2741 2914 Size: 53 83 186 936 824 129 255 173 Cuts at: 2914 3457 3839 4474 275 Size: 543 382 635 1451 Fragments arranged by size: 1451 936 824 635 543 382 255 186 173 129 83 53 BsaWI w'CCGG_w Cuts at: 4083 4481 4755 4902 5313 4083 Size: 398 274 147 411 4420 Fragments arranged by size: 4420 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5425 Fragments arranged by size: 5425 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2090 2531 2693 2708 2711 2714 2744 2765 Size: 904 441 162 15 3 3 30 21 Cuts at: 2765 1186 Size: 4071 Fragments arranged by size: 4071 904 441 162 30 21 15 3 3 BseYI C'CCAG_C Cuts at: 2758 4853 5349 2758 Size: 2095 496 3059 Fragments arranged by size: 3059 2095 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2030 2860 1163 Size: 573 294 830 3953 Fragments arranged by size: 3953 830 573 294 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2073 2336 2640 2793 3100 3861 Size: 275 186 72 263 304 153 307 761 Cuts at: 3861 4889 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 761 307 304 275 263 186 153 72 BsiHKAI G_wGCw'C Cuts at: 3711 3796 4867 3711 Size: 85 1071 4494 Fragments arranged by size: 4494 1071 85 BsmI GAATG_Cn' Cuts at: 2141 3161 3254 2141 Size: 1020 93 4537 Fragments arranged by size: 4537 1020 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2726 2726 Size: 5650 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2094 2849 2969 3711 3796 4867 1391 Size: 399 304 755 120 742 85 1071 2174 Fragments arranged by size: 2174 1071 755 742 399 304 120 85 BspEI T'CCGG_A Cuts at: 5313 5313 Size: 5650 BspHI T'CATG_A Cuts at: 3539 5269 3539 Size: 1730 3920 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2838 3005 844 Size: 1994 167 3489 Fragments arranged by size: 3489 1994 167 BsrBI CCG'CTC Cuts at: 1394 1823 2115 2287 2591 3537 1394 Size: 429 292 172 304 946 3507 Fragments arranged by size: 3507 946 429 304 292 172 BsrDI GCAATG_nn' Cuts at: 65 1248 4145 4319 65 Size: 1183 2897 174 1396 Fragments arranged by size: 2897 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2068 2331 2406 2792 4294 4481 1700 Size: 110 258 263 75 386 1502 187 2869 Fragments arranged by size: 2869 1502 386 263 258 187 110 75 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5650 BssHII G'CGCG_C Cuts at: 1925 2084 2608 1925 Size: 159 524 4967 Fragments arranged by size: 4967 524 159 BssSI C'ACGA_G Cuts at: 3710 4722 3710 Size: 1012 4638 BstAPI GCAn_nnn'nTGC Cuts at: 794 2245 794 Size: 1451 4199 BstBI TT'CG_AA Cuts at: 4454 4454 Size: 5650 BstEII G'GTnAC_C Cuts at: 1184 2887 1184 Size: 1703 3947 BstXI CCAn_nnnn'nTGG Cuts at: 2224 2224 Size: 5650 BstYI r'GATC_y Cuts at: 1972 2211 2262 2980 3347 3732 3749 5201 5287 Size: 239 51 718 367 385 17 1452 86 Cuts at: 5287 5299 5645 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 718 385 367 346 239 86 51 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2437 513 Size: 552 939 433 3726 Fragments arranged by size: 3726 939 552 433 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2911 487 Size: 683 56 1685 3226 Fragments arranged by size: 3226 1685 683 56 BtsI GCAGTG_nn' Cuts at: 3166 3960 3980 3166 Size: 794 20 4836 Fragments arranged by size: 4836 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5650 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5615 DraI TTT'AAA Cuts at: 2954 2954 Size: 5650 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2740 4657 809 Size: 507 1424 1917 1802 Fragments arranged by size: 1917 1802 1424 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2070 2116 2333 Size: 54 1425 50 275 18 240 46 217 Cuts at: 2333 2434 2637 2790 3097 3986 5615 8 Size: 101 203 153 307 889 1629 43 Fragments arranged by size: 1629 1425 889 307 275 240 217 203 153 101 54 50 46 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2070 2333 2637 2790 3097 1537 Size: 275 258 263 304 153 307 4090 Fragments arranged by size: 4090 307 304 275 263 258 153 EarI CTCTTCn'nnn_ Cuts at: 1353 2685 2721 3580 1353 Size: 1332 36 859 3423 Fragments arranged by size: 3423 1332 859 36 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2185 2297 2324 4237 4611 4757 Size: 1064 138 596 112 27 1913 374 146 Cuts at: 4757 387 Size: 1280 Fragments arranged by size: 1913 1280 1064 596 374 146 138 112 27 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3713 4310 5097 5365 1239 Size: 393 2081 597 787 268 1524 Fragments arranged by size: 2081 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2060 2462 2060 Size: 402 5248 EcoRI G'AATT_C Cuts at: 2959 3337 2959 Size: 378 5272 FseI GG_CCGG'CC Cuts at: 2072 2796 2072 Size: 724 4926 FspI TGC'GCA Cuts at: 4156 4156 Size: 5650 HaeII r_GCGC'y Cuts at: 1678 2224 2360 2489 2811 2917 4797 1678 Size: 546 136 129 322 106 1880 2531 Fragments arranged by size: 2531 1880 546 322 136 129 106 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4291 4323 4365 4397 1373 Size: 32 135 32 2719 32 42 32 2626 Fragments arranged by size: 2719 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2994 3240 669 Size: 988 281 1056 246 3079 Fragments arranged by size: 3079 1056 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5650 HpaI GTT'AAC Cuts at: 3240 3240 Size: 5650 KasI G'GCGC_C Cuts at: 2356 2485 2913 2356 Size: 129 428 5093 Fragments arranged by size: 5093 428 129 KpnI G_GTAC'C Cuts at: 2975 2975 Size: 5650 MfeI C'AATT_G Cuts at: 3249 3249 Size: 5650 MluI A'CGCG_T Cuts at: 5526 5526 Size: 5650 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2649 4764 4948 1305 Size: 1344 2115 184 2007 Fragments arranged by size: 2115 2007 1344 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3828 Fragments arranged by size: 3828 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3608 3967 4126 518 Size: 648 2442 359 159 2042 Fragments arranged by size: 2442 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2017 2235 2242 2428 2439 2456 2711 3066 Size: 411 218 7 186 11 17 255 355 Cuts at: 3066 3743 4891 5136 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 411 355 255 245 218 186 17 11 7 NaeI GCC'GGC Cuts at: 2070 2408 2794 2070 Size: 338 386 4926 Fragments arranged by size: 4926 386 338 NarI GG'CG_CC Cuts at: 2357 2486 2914 2357 Size: 129 428 5093 Fragments arranged by size: 5093 428 129 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4159 Fragments arranged by size: 4159 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5650 NgoMIV G'CCGG_C Cuts at: 2068 2406 2792 2068 Size: 338 386 4926 Fragments arranged by size: 4926 386 338 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5650 NotI GC'GGCC_GC Cuts at: 2637 3097 2637 Size: 460 5190 NruI TCG'CGA Cuts at: 2820 2820 Size: 5650 NspI r_CATG'y Cuts at: 1132 1229 2200 3008 4553 1132 Size: 97 971 808 1545 2229 Fragments arranged by size: 2229 1545 971 808 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5650 PciI A'CATG_T Cuts at: 4549 4549 Size: 5650 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5650 PfoI T'CCnGG_A Cuts at: 2865 2865 Size: 5650 PmeI GTTT'AAAC Cuts at: 2954 2954 Size: 5650 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3719 3751 5232 5264 3719 Size: 32 1481 32 4105 Fragments arranged by size: 4105 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2060 2060 Size: 5650 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5650 PsiI TTA'TAA Cuts at: 3220 3220 Size: 5650 PstI C_TGCA'G Cuts at: 830 2250 2807 2835 3002 830 Size: 1420 557 28 167 3478 Fragments arranged by size: 3478 1420 557 167 28 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5650 PvuII CAG'CTG Cuts at: 2242 2428 2242 Size: 186 5464 SacII CC_GC'GG Cuts at: 2440 2440 Size: 5650 SalI G'TCGA_C Cuts at: 1936 2992 1936 Size: 1056 4594 SanDI GG'GwC_CC Cuts at: 2060 2060 Size: 5650 SbfI CC_TGCA'GG Cuts at: 3002 3002 Size: 5650 ScaI AGT'ACT Cuts at: 3898 5610 3898 Size: 1712 3938 SexAI A'CCwGG_T Cuts at: 2934 2934 Size: 5650 SfcI C'TryA_G Cuts at: 826 1045 2246 2803 2831 2998 4133 4814 5005 Size: 219 1201 557 28 167 1135 681 191 Cuts at: 5005 826 Size: 1471 Fragments arranged by size: 1471 1201 1135 681 557 219 191 167 28 SfiI GGCCn_nnn'nGGCC Cuts at: 2442 2793 2442 Size: 351 5299 SfoI GGC'GCC Cuts at: 2358 2487 2915 2358 Size: 129 428 5093 Fragments arranged by size: 5093 428 129 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5650 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5650 SmaI CCC'GGG Cuts at: 1872 2058 2065 2419 2550 2977 1872 Size: 186 7 354 131 427 4545 Fragments arranged by size: 4545 427 354 186 131 7 SmlI C'TyrA_G Cuts at: 820 1017 1943 2297 2411 3754 4655 4917 820 Size: 197 926 354 114 1343 901 262 1553 Fragments arranged by size: 1553 1343 926 901 354 262 197 114 SnaBI TAC'GTA Cuts at: 493 3441 493 Size: 2948 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5650 SphI G_CATG'C Cuts at: 1132 3008 1132 Size: 1876 3774 SrfI GCCC'GGGC Cuts at: 2419 2419 Size: 5650 SspI AAT'ATT Cuts at: 5 52 3574 5 Size: 47 3522 2081 Fragments arranged by size: 3522 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5650 StyI C'CwwG_G Cuts at: 513 1065 2004 2364 2614 3089 513 Size: 552 939 360 250 475 3074 Fragments arranged by size: 3074 939 552 475 360 250 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3677 3694 3847 4032 4477 325 Size: 1255 2097 17 153 185 445 1498 Fragments arranged by size: 2097 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3896 5608 96 Size: 274 80 33 51 1443 1919 1712 138 Fragments arranged by size: 1919 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5610 5642 5610 Size: 32 5618 Tth111I GACn'n_nGTC Cuts at: 1316 2665 1316 Size: 1349 4301 XbaI T'CTAG_A Cuts at: 2986 2986 Size: 5650 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5650 XmaI C'CCGG_G Cuts at: 1870 2056 2063 2417 2548 2975 1870 Size: 186 7 354 131 427 4545 Fragments arranged by size: 4545 427 354 186 131 7 XmnI GAAnn'nnTTC Cuts at: 2141 3779 2141 Size: 1638 4012 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3458 276 Size: 53 83 186 936 1924 2468 Fragments arranged by size: 2468 1924 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FspAI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SapI SwaI XcmI