(Linear) (Six-base) MAPSORT of: KIBB9650.seq Check: 9197 from: 1 to: 945 KIBB9650.orf With 182 enzymes: SgfI * March 22, 2012 15:46 .. AlwNI CAG_nnn'CTG Cuts at: 0 240 709 945 Size: 240 469 236 Fragments arranged by size: 469 240 236 AvaI C'yCGr_G Cuts at: 0 51 58 105 412 484 543 587 837 Size: 51 7 47 307 72 59 44 250 Cuts at: 837 877 945 Size: 40 68 Fragments arranged by size: 307 250 72 68 59 51 47 44 40 7 BanI G'GyrC_C Cuts at: 0 351 480 908 945 Size: 351 129 428 37 Fragments arranged by size: 428 351 129 37 BanII G_rGCy'C Cuts at: 0 89 945 Size: 89 856 BbeI G_GCGC'C Cuts at: 0 355 484 912 945 Size: 355 129 428 33 Fragments arranged by size: 428 355 129 33 BbsI GAAGACnn'nnnn_ Cuts at: 0 506 945 Size: 506 439 BbvCI CC'TCA_GC Cuts at: 0 710 945 Size: 710 235 BglI GCCn_nnn'nGGC Cuts at: 0 437 788 945 Size: 437 351 157 Fragments arranged by size: 437 351 157 BglII A'GATC_T Cuts at: 0 206 945 Size: 206 739 Bme1580I G_kGCm'C Cuts at: 0 844 945 Size: 844 101 BmgBI CAC'GTC Cuts at: 0 845 945 Size: 845 100 Bpu10I CC'TnA_GC Cuts at: 0 710 945 Size: 710 235 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 277 391 945 Size: 277 114 554 Fragments arranged by size: 554 277 114 BsaI GGTCTCn'nnnn_ Cuts at: 0 532 945 Size: 532 413 BsaHI Gr'CG_yC Cuts at: 0 352 481 736 909 945 Size: 352 129 255 173 36 Fragments arranged by size: 352 255 173 129 36 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 85 526 688 703 706 709 739 760 Size: 85 441 162 15 3 3 30 21 Cuts at: 760 945 Size: 185 Fragments arranged by size: 441 185 162 85 30 21 15 3 3 BseYI C'CCAG_C Cuts at: 0 753 945 Size: 753 192 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 25 855 945 Size: 25 830 90 Fragments arranged by size: 830 90 25 BsiEI CG_ry'CG Cuts at: 0 68 331 635 788 945 Size: 68 263 304 153 157 Fragments arranged by size: 304 263 157 153 68 BsmI GAATG_Cn' Cuts at: 0 136 945 Size: 136 809 BsmBI CGTCTCn'nnnn_ Cuts at: 0 721 945 Size: 721 224 Bsp1286I G_dGCh'C Cuts at: 0 89 844 945 Size: 89 755 101 Fragments arranged by size: 755 101 89 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 833 945 Size: 833 112 BsrBI CCG'CTC Cuts at: 0 110 282 586 945 Size: 110 172 304 359 Fragments arranged by size: 359 304 172 110 BsrFI r'CCGG_y Cuts at: 0 63 326 401 787 945 Size: 63 263 75 386 158 Fragments arranged by size: 386 263 158 75 63 BssHII G'CGCG_C Cuts at: 0 79 603 945 Size: 79 524 342 Fragments arranged by size: 524 342 79 BstAPI GCAn_nnn'nTGC Cuts at: 0 240 945 Size: 240 705 BstEII G'GTnAC_C Cuts at: 0 882 945 Size: 882 63 BstXI CCAn_nnnn'nTGG Cuts at: 0 219 945 Size: 219 726 BstYI r'GATC_y Cuts at: 0 206 257 945 Size: 206 51 688 Fragments arranged by size: 688 206 51 BtgI C'CryG_G Cuts at: 0 432 945 Size: 432 513 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 906 945 Size: 906 39 DrdI GACnn_nn'nnGTC Cuts at: 0 735 945 Size: 735 210 EaeI y'GGCC_r Cuts at: 0 65 111 328 429 632 785 945 Size: 65 46 217 101 203 153 160 Fragments arranged by size: 217 203 160 153 101 65 46 EagI C'GGCC_G Cuts at: 0 65 328 632 785 945 Size: 65 263 304 153 160 Fragments arranged by size: 304 263 160 153 65 EarI CTCTTCn'nnn_ Cuts at: 0 680 716 945 Size: 680 36 229 Fragments arranged by size: 680 229 36 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 180 292 319 945 Size: 180 112 27 626 Fragments arranged by size: 626 180 112 27 EcoO109I rG'GnC_Cy Cuts at: 0 55 457 945 Size: 55 402 488 Fragments arranged by size: 488 402 55 FseI GG_CCGG'CC Cuts at: 0 67 791 945 Size: 67 724 154 Fragments arranged by size: 724 154 67 HaeII r_GCGC'y Cuts at: 0 219 355 484 806 912 945 Size: 219 136 129 322 106 33 Fragments arranged by size: 322 219 136 129 106 33 KasI G'GCGC_C Cuts at: 0 351 480 908 945 Size: 351 129 428 37 Fragments arranged by size: 428 351 129 37 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 644 945 Size: 644 301 MspA1I CmG'CkG Cuts at: 0 12 230 237 423 434 451 706 945 Size: 12 218 7 186 11 17 255 239 Fragments arranged by size: 255 239 218 186 17 12 11 7 NaeI GCC'GGC Cuts at: 0 65 403 789 945 Size: 65 338 386 156 Fragments arranged by size: 386 338 156 65 NarI GG'CG_CC Cuts at: 0 352 481 909 945 Size: 352 129 428 36 Fragments arranged by size: 428 352 129 36 NgoMIV G'CCGG_C Cuts at: 0 63 401 787 945 Size: 63 338 386 158 Fragments arranged by size: 386 338 158 63 NotI GC'GGCC_GC Cuts at: 0 632 945 Size: 632 313 NruI TCG'CGA Cuts at: 0 815 945 Size: 815 130 NspI r_CATG'y Cuts at: 0 195 945 Size: 195 750 PfoI T'CCnGG_A Cuts at: 0 860 945 Size: 860 85 PpuMI rG'GwC_Cy Cuts at: 0 55 945 Size: 55 890 PstI C_TGCA'G Cuts at: 0 245 802 830 945 Size: 245 557 28 115 Fragments arranged by size: 557 245 115 28 PvuII CAG'CTG Cuts at: 0 237 423 945 Size: 237 186 522 Fragments arranged by size: 522 237 186 SacII CC_GC'GG Cuts at: 0 435 945 Size: 435 510 SanDI GG'GwC_CC Cuts at: 0 55 945 Size: 55 890 SexAI A'CCwGG_T Cuts at: 0 929 945 Size: 929 16 SfcI C'TryA_G Cuts at: 0 241 798 826 945 Size: 241 557 28 119 Fragments arranged by size: 557 241 119 28 SfiI GGCCn_nnn'nGGCC Cuts at: 0 437 788 945 Size: 437 351 157 Fragments arranged by size: 437 351 157 SfoI GGC'GCC Cuts at: 0 353 482 910 945 Size: 353 129 428 35 Fragments arranged by size: 428 353 129 35 SmaI CCC'GGG Cuts at: 0 53 60 414 545 945 Size: 53 7 354 131 400 Fragments arranged by size: 400 354 131 53 7 SmlI C'TyrA_G Cuts at: 0 292 406 945 Size: 292 114 539 Fragments arranged by size: 539 292 114 SrfI GCCC'GGGC Cuts at: 0 414 945 Size: 414 531 StyI C'CwwG_G Cuts at: 0 359 609 945 Size: 359 250 336 Fragments arranged by size: 359 336 250 Tth111I GACn'n_nGTC Cuts at: 0 660 945 Size: 660 285 XmaI C'CCGG_G Cuts at: 0 51 58 412 543 945 Size: 51 7 354 131 402 Fragments arranged by size: 402 354 131 51 7 XmnI GAAnn'nnTTC Cuts at: 0 136 945 Size: 136 809 Enzymes that do cut: AlwNI AvaI BanI BanII BbeI BbsI BbvCI BglI BglII Bme1580I BmgBI Bpu10I BpuEI BsaI BsaHI BseRI BseYI BsgI BsiEI BsmI BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI BstEII BstXI BstYI BtgI BtgZI DrdI EaeI EagI EarI EciI EcoO109I FseI HaeII KasI MmeI MspA1I NaeI NarI NgoMIV NotI NruI NspI PfoI PpuMI PstI PvuII SacII SanDI SexAI SfcI SfiI SfoI SmaI SmlI SrfI StyI Tth111I XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BcgI BciVI BclI BfrBI BlpI BmrI BmtI BplI BpmI BsaAI BsaBI BsaWI BsaXI BsiHKAI BsiWI BspEI BspHI BsrDI BsrGI BssSI BstBI BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI MscI MslI NcoI NdeI NheI NsiI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SalI SapI SbfI ScaI SgfI SgrAI SnaBI SpeI SphI SspI StuI SwaI TaqII TatI TstI XbaI XcmI XhoI ZraI