(Linear) (Six-base) MAPSORT of: KIEE3962.seq Check: 71 from: 1 to: 1533 KIEE3962.orf With 182 enzymes: SgfI * March 22, 2012 16:59 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 41 291 444 473 567 1533 Size: 41 250 153 29 94 966 Fragments arranged by size: 966 250 153 94 41 29 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1022 1533 Size: 1022 511 AfeI AGC'GCT Cuts at: 0 1399 1533 Size: 1399 134 AflIII A'CryG_T Cuts at: 0 1262 1533 Size: 1262 271 AleI CACnn'nnGTG Cuts at: 0 95 415 494 1007 1363 1533 Size: 95 320 79 513 356 170 Fragments arranged by size: 513 356 320 170 95 79 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 512 1533 Size: 512 1021 AlwNI CAG_nnn'CTG Cuts at: 0 16 1060 1192 1220 1533 Size: 16 1044 132 28 313 Fragments arranged by size: 1044 313 132 28 16 ApaLI G'TGCA_C Cuts at: 0 1426 1533 Size: 1426 107 AvaI C'yCGr_G Cuts at: 0 374 381 483 1096 1275 1447 1533 Size: 374 7 102 613 179 172 86 Fragments arranged by size: 613 374 179 172 102 86 7 BamHI G'GATC_C Cuts at: 0 946 1533 Size: 946 587 BanI G'GyrC_C Cuts at: 0 222 385 488 696 799 1009 1168 1533 Size: 222 163 103 208 103 210 159 365 Fragments arranged by size: 365 222 210 208 163 159 103 103 BanII G_rGCy'C Cuts at: 0 381 767 1417 1533 Size: 381 386 650 116 Fragments arranged by size: 650 386 381 116 BbsI GAAGACnn'nnnn_ Cuts at: 0 973 1473 1533 Size: 973 500 60 Fragments arranged by size: 973 500 60 BbvCI CC'TCA_GC Cuts at: 0 569 836 1533 Size: 569 267 697 Fragments arranged by size: 697 569 267 BciVI GTATCCnnnnn_n' Cuts at: 0 873 1533 Size: 873 660 BlpI GC'TnA_GC Cuts at: 0 1193 1533 Size: 1193 340 Bme1580I G_kGCm'C Cuts at: 0 138 227 388 422 491 1014 1430 1533 Size: 138 89 161 34 69 523 416 103 Fragments arranged by size: 523 416 161 138 103 89 69 34 BmgBI CAC'GTC Cuts at: 0 102 478 1533 Size: 102 376 1055 Fragments arranged by size: 1055 376 102 BmrI ACTGGGnnnn_n' Cuts at: 0 1103 1533 Size: 1103 430 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1075 1522 1533 Size: 1075 447 11 Fragments arranged by size: 1075 447 11 Bpu10I CC'TnA_GC Cuts at: 0 569 836 1533 Size: 569 267 697 Fragments arranged by size: 697 569 267 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 79 1533 Size: 79 1454 BsaI GGTCTCn'nnnn_ Cuts at: 0 55 643 907 1533 Size: 55 588 264 626 Fragments arranged by size: 626 588 264 55 BsaAI yAC'GTr Cuts at: 0 1205 1533 Size: 1205 328 BsaWI w'CCGG_w Cuts at: 0 216 1533 Size: 216 1317 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 775 1533 Size: 775 758 BseYI C'CCAG_C Cuts at: 0 826 1119 1533 Size: 826 293 414 Fragments arranged by size: 826 414 293 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 458 485 755 1233 1533 Size: 458 27 270 478 300 Fragments arranged by size: 478 458 300 270 27 BsiEI CG_ry'CG Cuts at: 0 158 314 1533 Size: 158 156 1219 Fragments arranged by size: 1219 158 156 BsiHKAI G_wGCw'C Cuts at: 0 308 1417 1430 1533 Size: 308 1109 13 103 Fragments arranged by size: 1109 308 103 13 BsmI GAATG_Cn' Cuts at: 0 113 355 1533 Size: 113 242 1178 Fragments arranged by size: 1178 242 113 Bsp1286I G_dGCh'C Cuts at: 0 138 227 308 381 388 422 491 767 Size: 138 89 81 73 7 34 69 276 Cuts at: 767 1014 1417 1430 1533 Size: 247 403 13 103 Fragments arranged by size: 403 276 247 138 103 89 81 73 69 34 13 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 41 291 444 473 567 1343 1533 Size: 41 250 153 29 94 776 190 Fragments arranged by size: 776 250 190 153 94 41 29 BsrFI r'CCGG_y Cuts at: 0 157 1360 1533 Size: 157 1203 173 Fragments arranged by size: 1203 173 157 BsrGI T'GTAC_A Cuts at: 0 260 1533 Size: 260 1273 BssHII G'CGCG_C Cuts at: 0 1368 1452 1454 1493 1533 Size: 1368 84 2 39 40 Fragments arranged by size: 1368 84 40 39 2 BstAPI GCAn_nnn'nTGC Cuts at: 0 237 1533 Size: 237 1296 BstYI r'GATC_y Cuts at: 0 203 946 1533 Size: 203 743 587 Fragments arranged by size: 743 587 203 Bsu36I CC'TnA_GG Cuts at: 0 1164 1533 Size: 1164 369 BtgI C'CryG_G Cuts at: 0 599 1154 1533 Size: 599 555 379 Fragments arranged by size: 599 555 379 BtsI GCAGTG_nn' Cuts at: 0 734 1533 Size: 734 799 EaeI y'GGCC_r Cuts at: 0 155 192 311 1533 Size: 155 37 119 1222 Fragments arranged by size: 1222 155 119 37 EagI C'GGCC_G Cuts at: 0 155 311 1533 Size: 155 156 1222 Fragments arranged by size: 1222 156 155 EarI CTCTTCn'nnn_ Cuts at: 0 997 1405 1533 Size: 997 408 128 Fragments arranged by size: 997 408 128 EcoICRI GAG'CTC Cuts at: 0 1415 1533 Size: 1415 118 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1022 1075 1522 1533 Size: 1022 53 447 11 Fragments arranged by size: 1022 447 53 11 EcoO109I rG'GnC_Cy Cuts at: 0 574 1128 1533 Size: 574 554 405 Fragments arranged by size: 574 554 405 FspI TGC'GCA Cuts at: 0 112 440 1435 1533 Size: 112 328 995 98 Fragments arranged by size: 995 328 112 98 FspAI rTGC'GCAy Cuts at: 0 112 1435 1533 Size: 112 1323 98 Fragments arranged by size: 1323 112 98 HaeII r_GCGC'y Cuts at: 0 522 550 890 1401 1509 1533 Size: 522 28 340 511 108 24 Fragments arranged by size: 522 511 340 108 28 24 HindIII A'AGCT_T Cuts at: 0 122 1533 Size: 122 1411 MslI CAynn'nnrTG Cuts at: 0 95 415 494 1007 1363 1533 Size: 95 320 79 513 356 170 Fragments arranged by size: 513 356 320 170 95 79 MspA1I CmG'CkG Cuts at: 0 143 246 266 444 540 601 835 1119 Size: 143 103 20 178 96 61 234 284 Cuts at: 1119 1533 Size: 414 Fragments arranged by size: 414 284 234 178 143 103 96 61 20 NaeI GCC'GGC Cuts at: 0 159 1533 Size: 159 1374 NgoMIV G'CCGG_C Cuts at: 0 157 1533 Size: 157 1376 NspI r_CATG'y Cuts at: 0 1266 1434 1533 Size: 1266 168 99 Fragments arranged by size: 1266 168 99 PasI CC'CwG_GG Cuts at: 0 973 1015 1533 Size: 973 42 518 Fragments arranged by size: 973 518 42 PciI A'CATG_T Cuts at: 0 1262 1533 Size: 1262 271 PflMI CCAn_nnn'nTGG Cuts at: 0 468 1533 Size: 468 1065 PfoI T'CCnGG_A Cuts at: 0 1240 1533 Size: 1240 293 PpuMI rG'GwC_Cy Cuts at: 0 1128 1533 Size: 1128 405 PshAI GACnn'nnGTC Cuts at: 0 919 1533 Size: 919 614 PspXI vC'TCGA_Gb Cuts at: 0 374 1533 Size: 374 1159 PstI C_TGCA'G Cuts at: 0 12 38 302 1533 Size: 12 26 264 1231 Fragments arranged by size: 1231 264 26 12 PvuII CAG'CTG Cuts at: 0 246 444 540 835 1119 1533 Size: 246 198 96 295 284 414 Fragments arranged by size: 414 295 284 246 198 96 SacI G_AGCT'C Cuts at: 0 1417 1533 Size: 1417 116 SacII CC_GC'GG Cuts at: 0 602 1533 Size: 602 931 SapI GCTCTTCn'nnn_ Cuts at: 0 1405 1533 Size: 1405 128 SbfI CC_TGCA'GG Cuts at: 0 302 1533 Size: 302 1231 ScaI AGT'ACT Cuts at: 0 1113 1533 Size: 1113 420 SfcI C'TryA_G Cuts at: 0 8 34 298 792 1533 Size: 8 26 264 494 741 Fragments arranged by size: 741 494 264 26 8 SgrAI Cr'CCGG_yG Cuts at: 0 1360 1533 Size: 1360 173 SmaI CCC'GGG Cuts at: 0 383 485 1533 Size: 383 102 1048 Fragments arranged by size: 1048 383 102 SmlI C'TyrA_G Cuts at: 0 58 374 1533 Size: 58 316 1159 Fragments arranged by size: 1159 316 58 SnaBI TAC'GTA Cuts at: 0 1205 1533 Size: 1205 328 StyI C'CwwG_G Cuts at: 0 563 1533 Size: 563 970 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 629 667 789 997 1533 Size: 629 38 122 208 536 Fragments arranged by size: 629 536 208 122 38 TatI w'GTAC_w Cuts at: 0 260 1111 1533 Size: 260 851 422 Fragments arranged by size: 851 422 260 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 155 187 1533 Size: 155 32 1346 Fragments arranged by size: 1346 155 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1116 1533 Size: 1116 417 XhoI C'TCGA_G Cuts at: 0 374 1533 Size: 374 1159 XmaI C'CCGG_G Cuts at: 0 381 483 1533 Size: 381 102 1050 Fragments arranged by size: 1050 381 102 Enzymes that do cut: AarI AcuI AfeI AflIII AleI AlfI AlwNI ApaLI AvaI BamHI BanI BanII BbsI BbvCI BciVI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrFI BsrGI BssHII BstAPI BstYI Bsu36I BtgI BtsI EaeI EagI EarI EcoICRI Eco57MI EcoO109I FspI FspAI HaeII HindIII MslI MspA1I NaeI NgoMIV NspI PasI PciI PflMI PfoI PpuMI PshAI PspXI PstI PvuII SacI SacII SapI SbfI ScaI SfcI SgrAI SmaI SmlI SnaBI StyI TaqII TatI TstI XcmI XhoI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AgeI AhdI AloI ApaI ApoI AscI AseI AsiSI AvrII BaeI BbeI BcgI BclI BfrBI BglI BglII BmtI BplI BsaBI BsaHI BsaXI BsiWI BsmBI BspEI BspHI BsrBI BsrDI BssSI BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EciI EcoNI EcoRI EcoRV FalI FseI Hin4I HincII HpaI KasI KpnI MfeI MluI MmeI MscI NarI NcoI NdeI NheI NotI NruI NsiI PacI PmeI PmlI PpiI PsiI PspOMI PsrI PvuI RsrII SalI SanDI SexAI SfiI SfoI SgfI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XmnI ZraI