Restriction map for
pFN21AB7733
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII BanI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BglI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstEII BstPI
BstUI BstXI BstZ17I Bsu36I Cac8I
CelII CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoT22I FauI FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI HpaII KasI KpnI KspI
MamI MfeI MluI MroI MspA1I
MspI MunI MvnI NaeI NarI
NciI NgoMIV NheI NotI NruI
NsiI PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SwaI Tth111I Van91I
XbaI XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AfaI 898 GTAC GT^AC
AflII 447 CTTAAG C^TTAAG
Asp700I 1095 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 136 GTGCTC G[AT]GC[AT]^C
AvrII 682 CCTAGG C^CTAGG
BalI 285 TGGCCA TGG^CCA
BamHI 910 GGATCC G^GATCC
BfrI 447 CTTAAG C^TTAAG
BlnI 682 CCTAGG C^CTAGG
BmyI 136 GTGCTC G[AGT]GC[ACT]^C
BpmI 265 CTGGAG CTGGAG(16/14)
BsaI 1151 GGTCTC GGTCTC(1/5)
BsiHKAI 136 GTGCTC G[AT]GC[AT]^C
BsmAI 1152 GTCTC GTCTC(1/5)
Bsp1286I 136 GTGCTC G[AGT]GC[ACT]^C
BstAPI 177 GCAGGAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 597 TTCGAA TT^CGAA
DraII 87 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 915 CCATGG C^C[GA][CT]GG
EaeI 285 TGGCCA [CT]^GGCC[GA]
EcoO109I 87 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 1053 GATATC GAT^ATC
HindIII 1292 AAGCTT A^AGCTT
MaeII 401 ACGT A^CGT
MluNI 285 TGGCCA TGG^CCA
MscI 285 TGGCCA TGG^CCA
MwoI 177 GCAGGAATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 915 CCATGG C^CATGG
NspV 597 TTCGAA TT^CGAA
PaeR7I 608 CTCGAG C^TCGAG
PpuMI 87 AGGACCT [GA]G^G[AT]CC[CT]
RsaI 898 GTAC GT^AC
SfaNI 1071 GCATC GCATC(5/9)
SfuI 597 TTCGAA TT^CGAA
SspI 622 AATATT AAT^ATT
StuI 685 AGGCCT AGG^CCT
XhoI 608 CTCGAG C^TCGAG
XmnI 1095 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcsI 63 AAATTC [GA]^AATT[CT]
882 AAATTC
AflIII 889 ACATGT A^C[GA][CT]GT
1189 ACATGT
ApoI 63 AAATTC [GA]^AATT[CT]
882 AAATTC
AvaI 608 CTCGAG C^[CT]CG[GA]G
1027 CCCGAG
BbsI 358 GAAGAC GAAGAC(2/6)
995 GAAGAC
BbvI 1109 GCAGC GCAGC(8/12)
216 GCTGC
BglII 9 AGATCT A^GATCT
754 AGATCT
BpuAI 358 GAAGAC GAAGAC(2/6)
995 GAAGAC
BsmI 483 GAATGC GAATGC(1/-1)
946 GAATGC
BsoBI 608 CTCGAG C^[CT]CG[GA]G
1027 CCCGAG
BspHI 1155 TCATGA T^CATGA
1251 TCATGA
BspLU11I 889 ACATGT A^CATGT
1189 ACATGT
BsrDI 293 GCAATG GCAATG(2/0)
872 GCAATG
BsrI 305 CCAGT ACTGG(1/-1)
1218 CCAGT
BssKI 264 CCTGG ^CC[ACGT]GG
666 CCAGG
BstNI 264 CCTGG CC^[AT]GG
666 CCAGG
EarI 670 GAAGAG CTCTTC(1/4)
691 GAAGAG
EcoRII 264 CCTGG ^CC[AT]GG
666 CCAGG
Fnu4HI 216 GCTGC GC^[ACGT]GC
1109 GCAGC
HphI 804 GGTGA GGTGA(8/7)
987 TCACC
ItaI 216 GCTGC GC^[ACGT]GC
1109 GCAGC
Ksp632I 670 GAAGAG CTCTTC(1/4)
691 GAAGAG
MaeIII 745 GTCAC ^GT[ACGT]AC
974 GTCAC
MseI 448 TTAA T^TAA
962 TTAA
MvaI 264 CCTGG CC^[AT]GG
666 CCAGG
NdeI 167 CATATG CA^TATG
1240 CATATG
NlaIV 766 GGGTCC GG[ACGT]^[ACGT]CC
910 GGATCC
RcaI 1155 TCATGA T^CATGA
1251 TCATGA
ScrFI 264 CCTGG CC^[ACGT]GG
666 CCAGG
TaqI 598 TCGA T^CGA
609 TCGA
Tru9I 448 TTAA T^TAA
962 TTAA
TseI 216 GCTGC G^C[AT]GC
1109 GCAGC
Tsp45I 745 GTCAC ^GT[GC]AC
974 GTCAC
TthHB8I 598 TCGA T^CGA
609 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AluI AlwI AvaII BfaI
BsaJI BsiYI BslI BsmFI BspMI
BstF5I BstYI Cfr13I DdeI DpnII
EcoT14I FokI HaeIII HinfI MaeI
MboI MboII MflI MnlI MslI
NdeII NlaIII NspI PleI Sau3AI
Sau96I SmlI StyI TfiI Tsp509I
TspRI XhoII