(Circular) (Six-base) MAPSORT of: pFN21AB7733.seq Check: 9087 from: 1 to: 6004 pFN21AB7733 6004 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2401 2401 Size: 6004 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3814 278 Size: 53 83 186 936 2278 2468 Fragments arranged by size: 2468 2278 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3347 1937 Size: 1410 4594 Acc65I G'GTAC_C Cuts at: 3325 3325 Size: 6004 AclI AA'CG_TT Cuts at: 1563 4131 4504 1563 Size: 2568 373 3063 Fragments arranged by size: 3063 2568 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2715 3118 4067 5451 5719 1632 Size: 1083 403 949 1384 268 1917 Fragments arranged by size: 1917 1384 1083 949 403 268 AflII C'TTAA_G Cuts at: 820 1017 2452 820 Size: 197 1435 4372 Fragments arranged by size: 4372 1435 197 AflIII A'CryG_T Cuts at: 1203 2894 3194 4903 5880 1203 Size: 1691 300 1709 977 1327 Fragments arranged by size: 1709 1691 1327 977 300 AgeI A'CCGG_T Cuts at: 4835 4835 Size: 6004 AhdI GACnn_n'nnGTC Cuts at: 4733 4733 Size: 6004 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6004 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6004 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2085 2117 2085 Size: 32 5972 AlwNI CAG_nnn'CTG Cuts at: 3747 5319 3747 Size: 1572 4432 ApaLI G'TGCA_C Cuts at: 4061 5217 4061 Size: 1156 4848 ApoI r'AATT_y Cuts at: 1493 2068 2887 3313 3539 3691 5800 1493 Size: 575 819 426 226 152 2109 1697 Fragments arranged by size: 2109 1697 819 575 426 226 152 AseI AT'TA_AT Cuts at: 160 1032 4558 160 Size: 872 3526 1606 Fragments arranged by size: 3526 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6004 AvaI C'yCGr_G Cuts at: 1870 1943 2613 3032 3329 1870 Size: 73 670 419 297 4545 Fragments arranged by size: 4545 670 419 297 73 AvrII C'CTAG_G Cuts at: 2687 2687 Size: 6004 BamHI G'GATC_C Cuts at: 2915 3334 3701 2915 Size: 419 367 5218 Fragments arranged by size: 5218 419 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3325 4780 618 Size: 325 217 627 1538 1455 1842 Fragments arranged by size: 1842 1538 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3323 1391 Size: 1932 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2370 3007 928 Size: 971 471 637 3925 Fragments arranged by size: 3925 971 637 471 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4195 4229 1903 Size: 34 2258 34 3678 Fragments arranged by size: 3678 2258 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3896 5106 3896 Size: 1210 4794 BclI T'GATC_A Cuts at: 1548 1548 Size: 6004 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4615 136 Size: 107 122 71 631 605 2943 1525 Fragments arranged by size: 2943 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2014 2759 5999 2014 Size: 745 3240 2019 Fragments arranged by size: 3240 2019 745 BlpI GC'TnA_GC Cuts at: 3421 3421 Size: 6004 Bme1580I G_kGCm'C Cuts at: 1790 4065 5221 1790 Size: 2275 1156 2573 Fragments arranged by size: 2573 2275 1156 BmgBI CAC'GTC Cuts at: 1318 2407 1318 Size: 1089 4915 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3735 4693 442 Size: 416 53 83 399 24 2318 958 1753 Fragments arranged by size: 2318 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6004 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2863 2863 Size: 6004 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2291 4664 1239 Size: 1052 2373 2579 Fragments arranged by size: 2579 2373 1052 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2116 4129 4994 5292 2116 Size: 2013 865 298 2828 Fragments arranged by size: 2828 2013 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3162 4667 882 Size: 617 1663 1505 2219 Fragments arranged by size: 2219 1663 1505 617 BsaAI yAC'GTr Cuts at: 493 1204 3795 493 Size: 711 2591 2702 Fragments arranged by size: 2702 2591 711 BsaBI GATnn'nnATC Cuts at: 5998 5998 Size: 6004 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3811 4193 4828 275 Size: 53 83 186 936 2278 382 635 1451 Fragments arranged by size: 2278 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4437 4835 5109 5256 5667 4437 Size: 398 274 147 411 4774 Fragments arranged by size: 4774 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5779 Fragments arranged by size: 5779 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6004 BseYI C'CCAG_C Cuts at: 5207 5703 5207 Size: 496 5508 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5431 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3454 4215 5243 1540 Size: 275 186 1453 761 1028 2301 Fragments arranged by size: 2301 1453 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2145 4065 4150 5221 2145 Size: 1920 85 1071 2928 Fragments arranged by size: 2928 1920 1071 85 BsmI GAATG_Cn' Cuts at: 2494 2957 3515 3608 2494 Size: 463 558 93 4890 Fragments arranged by size: 4890 558 463 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2145 3323 4065 4150 5221 1391 Size: 399 355 1178 742 85 1071 2174 Fragments arranged by size: 2174 1178 1071 742 399 355 85 BspEI T'CCGG_A Cuts at: 5667 5667 Size: 6004 BspHI T'CATG_A Cuts at: 3160 3256 3893 5623 3160 Size: 96 637 1730 3541 Fragments arranged by size: 3541 1730 637 96 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2104 2401 3159 3359 844 Size: 1260 297 758 200 3489 Fragments arranged by size: 3489 1260 758 297 200 BsrBI CCG'CTC Cuts at: 1394 1823 3891 1394 Size: 429 2068 3507 Fragments arranged by size: 3507 2068 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2305 2884 4499 4673 65 Size: 1183 1057 579 1615 174 1396 Fragments arranged by size: 1615 1396 1183 1057 579 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4648 4835 1700 Size: 110 2838 187 2869 Fragments arranged by size: 2869 2838 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6004 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6004 BssSI C'ACGA_G Cuts at: 4064 5076 4064 Size: 1012 4992 BstAPI GCAn_nnn'nTGC Cuts at: 794 2188 794 Size: 1394 4610 BstBI TT'CG_AA Cuts at: 2603 4808 2603 Size: 2205 3799 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6004 BstYI r'GATC_y Cuts at: 1972 2014 2205 2759 2915 3334 3701 4086 4103 Size: 42 191 554 156 419 367 385 17 Cuts at: 4103 5555 5641 5653 5999 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 554 419 385 367 346 191 156 86 42 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2920 513 Size: 552 939 916 3597 Fragments arranged by size: 3597 939 916 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5265 Fragments arranged by size: 5265 683 56 BtsI GCAGTG_nn' Cuts at: 2293 2751 3520 4314 4334 2293 Size: 458 769 794 20 3963 Fragments arranged by size: 3963 794 769 458 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6004 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5969 DraI TTT'AAA Cuts at: 3308 3308 Size: 6004 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5011 809 Size: 507 3695 1802 Fragments arranged by size: 3695 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2290 3451 4340 Size: 54 1425 50 275 18 460 1161 889 Cuts at: 4340 5969 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1161 889 460 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3451 1537 Size: 275 1639 4090 Fragments arranged by size: 4090 1639 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2670 2691 3934 1353 Size: 1317 21 1243 3423 Fragments arranged by size: 3423 1317 1243 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4591 4965 5111 387 Size: 1064 138 3002 374 146 1280 Fragments arranged by size: 3002 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2291 2715 3118 4067 4664 5451 5719 Size: 393 659 424 403 949 597 787 268 Cuts at: 5719 1239 Size: 1524 Fragments arranged by size: 1524 949 787 659 597 424 403 393 268 EcoO109I rG'GnC_Cy Cuts at: 2093 2093 Size: 6004 EcoRI G'AATT_C Cuts at: 3313 3691 3313 Size: 378 5626 EcoRV GAT'ATC Cuts at: 3060 3060 Size: 6004 FspI TGC'GCA Cuts at: 4510 4510 Size: 6004 HaeII r_GCGC'y Cuts at: 1678 5151 1678 Size: 3473 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4645 4677 4719 4751 1373 Size: 32 135 32 3073 32 42 32 2626 Fragments arranged by size: 3073 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3348 3594 669 Size: 988 281 1410 246 3079 Fragments arranged by size: 3079 1410 988 281 246 HindIII A'AGCT_T Cuts at: 748 3297 748 Size: 2549 3455 HpaI GTT'AAC Cuts at: 3594 3594 Size: 6004 KpnI G_GTAC'C Cuts at: 3329 3329 Size: 6004 MfeI C'AATT_G Cuts at: 3603 3603 Size: 6004 MluI A'CGCG_T Cuts at: 5880 5880 Size: 6004 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5118 5302 1305 Size: 3813 184 2007 Fragments arranged by size: 3813 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2292 10 Size: 54 1425 343 460 3722 Fragments arranged by size: 3722 1425 460 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2298 2941 3009 3962 4321 4480 518 Size: 648 1132 643 68 953 359 159 2042 Fragments arranged by size: 2042 1132 953 648 643 359 159 68 MspA1I CmG'CkG Cuts at: 1606 3420 4097 5245 5490 1606 Size: 1814 677 1148 245 2120 Fragments arranged by size: 2120 1814 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2920 513 Size: 552 939 916 3597 Fragments arranged by size: 3597 939 916 552 NdeI CA'TA_TG Cuts at: 387 2173 3246 387 Size: 1786 1073 3145 Fragments arranged by size: 3145 1786 1073 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6004 NotI GC'GGCC_GC Cuts at: 3451 3451 Size: 6004 NspI r_CATG'y Cuts at: 1132 1229 2416 2898 3114 3121 3198 3362 4907 Size: 97 1187 482 216 7 77 164 1545 Cuts at: 4907 1132 Size: 2229 Fragments arranged by size: 2229 1545 1187 482 216 164 97 77 7 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6004 PciI A'CATG_T Cuts at: 2894 3194 4903 2894 Size: 300 1709 3995 Fragments arranged by size: 3995 1709 300 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6004 PfoI T'CCnGG_A Cuts at: 2268 2670 2268 Size: 402 5602 PmeI GTTT'AAAC Cuts at: 3308 3308 Size: 6004 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4073 4105 5586 5618 4073 Size: 32 1481 32 4459 Fragments arranged by size: 4459 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2093 2093 Size: 6004 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6004 PsiI TTA'TAA Cuts at: 3574 3574 Size: 6004 PspXI vC'TCGA_Gb Cuts at: 2613 2613 Size: 6004 PstI C_TGCA'G Cuts at: 830 3356 830 Size: 2526 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6004 SalI G'TCGA_C Cuts at: 1936 3346 1936 Size: 1410 4594 SbfI CC_TGCA'GG Cuts at: 3356 3356 Size: 6004 ScaI AGT'ACT Cuts at: 4252 5964 4252 Size: 1712 4292 SfcI C'TryA_G Cuts at: 826 1045 3352 4487 5168 5359 826 Size: 219 2307 1135 681 191 1471 Fragments arranged by size: 2307 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6004 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6004 SmaI CCC'GGG Cuts at: 1872 3331 1872 Size: 1459 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2131 2452 2613 4108 5009 5271 Size: 197 926 188 321 161 1495 901 262 Cuts at: 5271 820 Size: 1553 Fragments arranged by size: 1553 1495 926 901 321 262 197 188 161 SnaBI TAC'GTA Cuts at: 493 3795 493 Size: 3302 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6004 SphI G_CATG'C Cuts at: 1132 3362 1132 Size: 2230 3774 SspI AAT'ATT Cuts at: 5 52 2629 3928 5 Size: 47 2577 1299 2081 Fragments arranged by size: 2577 2081 1299 47 StuI AGG'CCT Cuts at: 1517 2692 1517 Size: 1175 4829 StyI C'CwwG_G Cuts at: 513 1065 2004 2089 2687 2794 2920 2958 3443 Size: 552 939 85 598 107 126 38 485 Cuts at: 3443 513 Size: 3074 Fragments arranged by size: 3074 939 598 552 485 126 107 85 38 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4031 4048 4201 4386 4831 325 Size: 1255 2451 17 153 185 445 1498 Fragments arranged by size: 2451 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4250 5962 96 Size: 274 80 33 51 1443 2273 1712 138 Fragments arranged by size: 2273 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2320 2352 5964 5996 2320 Size: 32 3612 32 2328 Fragments arranged by size: 3612 2328 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6004 XbaI T'CTAG_A Cuts at: 3340 3340 Size: 6004 XhoI C'TCGA_G Cuts at: 1943 2613 1943 Size: 670 5334 XmaI C'CCGG_G Cuts at: 1870 3329 1870 Size: 1459 4545 XmnI GAAnn'nnTTC Cuts at: 3104 4133 3104 Size: 1029 4975 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3812 276 Size: 53 83 186 936 2278 2468 Fragments arranged by size: 2468 2278 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI