################################## # NO CUT ENZYMES ( 146 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BseAI BseRI BsiHKAI BsiWI BsmBI Bsp1407I BspDI BspEI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HhaI HinP1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MroI MspA1I MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI SspBI SspI SwaI Tth111I Van91I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccII 839 CGCG CG^CG AcyI 649 GACGCC G[GA]^CG[CT]C AflII 1441 CTTAAG C^TTAAG AflIII 454 ACATGT A^C[GA][CT]GT AlwI 180 GGATC GGATC(4/5) Asp700I 612 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 623 TGGCCA TGG^CCA BanI 519 GGCACC G^G[CT][GA]CC BfrI 1441 CTTAAG C^TTAAG BmyI 981 GTGCCC G[AGT]GC[ACT]^C BsaHI 649 GACGCC G[GA]^CG[CT]C BsaWI 1283 ACCGGA [AT]^CCGG[AT] BsiEI 65 CGACCG CG[GA][CT]^CG BsmAI 1287 GAGAC GTCTC(1/5) BsmFI 77 GGGAC GGGAC(10/14) Bsp1286I 981 GTGCCC G[AGT]GC[ACT]^C BspHI 1227 TCATGA T^CATGA BspLU11I 454 ACATGT A^CATGT BspMI 595 ACCTGC ACCTGC(4/8) BstF5I 59 GGATG GGATG(2/0) BstUI 839 CGCG CG^CG BstYI 180 GGATCT [GA]^GATC[CT] DraII 77 GGGACCC [GA]G^G[ACGT]CC[CT] EcoNI 186 CCTTGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 77 GGGACCC [GA]G^G[ACGT]CC[CT] EcoT14I 423 CCAAGG C^C[AT][AT]GG FokI 59 GGATG GGATG(9/13) HgaI 649 GACGC GACGC(5/10) Hin1I 649 GACGCC G[GA]^CG[CT]C HphI 1278 TCACC GGTGA(8/7) MaeII 244 ACGT A^CGT MflI 180 GGATCT [GA]^GATC[CT] MluNI 623 TGGCCA TGG^CCA MscI 623 TGGCCA TGG^CCA MslI 451 CACACATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 839 CGCG CG^CG PpuMI 77 GGGACCC [GA]G^G[AT]CC[CT] PstI 597 CTGCAG CTGCA^G RcaI 1227 TCATGA T^CATGA SbfI 596 CCTGCAGG CCTGCA^GG SmaI 15 CCCGGG CCC^GGG Sse8387I 596 CCTGCAGG CCTGCA^GG StyI 423 CCAAGG C^C[AT][AT]GG TaqI 215 TCGA T^CGA TfiI 785 GAATC G^A[AT]TC Tsp45I 136 GTGAC ^GT[GC]AC TthHB8I 215 TCGA T^CGA XhoII 180 GGATCT [GA]^GATC[CT] XmaCI 15 CCCGGG C^CCGGG XmaI 15 CCCGGG C^CCGGG XmnI 612 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AcsI 302 GAATTT [GA]^AATT[CT] 913 AAATTC ApoI 302 GAATTT [GA]^AATT[CT] 913 AAATTC AvaI 4 CTCGGG C^[CT]CG[GA]G 15 CCCGGG BfaI 794 CTAG C^TAG 1604 CTAG BsoBI 4 CTCGGG C^[CT]CG[GA]G 15 CCCGGG BsrDI 899 GCAATG GCAATG(2/0) 1235 GCAATG EaeI 152 TGGCCG [CT]^GGCC[GA] 623 TGGCCA FauI 24 CCCGC CCCGC(4/6) 437 GCGGG MaeI 794 CTAG C^TAG 1604 CTAG MwoI 495 GCACCAGAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1019 GCCTCAATAGC NspI 454 ACATGT [GA]CATG^[CT] 832 GCATGT PleI 54 GACTC GAGTC(4/5) 367 GACTC SfaNI 222 GATGC GCATC(5/9) 1113 GCATC SfcI 101 CTACAG C^T[GA][CT]AG 597 CTGCAG SmlI 393 CTTGAG C^T[CT][GA]AG 1441 CTTAAG ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AciI AfaI AluI AvaII BbvI BcnI BpmI BsaJI BsgI BsiYI BslI BsmI BsrI BssKI BstNI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI HaeIII HapII HinfI HpaII ItaI MaeIII MboI MboII MnlI MseI MspI MvaI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI StuI Tru9I TseI Tsp509I TspRI