(Linear) (Six-base) MAPSORT of: KIEE3250.seq Check: 7624 from: 1 to: 1731 KIEE3250.orf With 182 enzymes: SgfI * March 22, 2012 16:27 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1341 1731 Size: 1341 390 AflII C'TTAA_G Cuts at: 0 1441 1731 Size: 1441 290 AflIII A'CryG_T Cuts at: 0 454 1731 Size: 454 1277 AleI CACnn'nnGTG Cuts at: 0 455 1731 Size: 455 1276 ApoI r'AATT_y Cuts at: 0 302 913 1731 Size: 302 611 818 Fragments arranged by size: 818 611 302 AvaI C'yCGr_G Cuts at: 0 4 15 1731 Size: 4 11 1716 Fragments arranged by size: 1716 11 4 BanI G'GyrC_C Cuts at: 0 519 1731 Size: 519 1212 Bme1580I G_kGCm'C Cuts at: 0 985 1731 Size: 985 746 BmrI ACTGGGnnnn_n' Cuts at: 0 738 1731 Size: 738 993 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 247 329 915 983 1067 1151 1235 1251 Size: 247 82 586 68 84 84 84 16 Cuts at: 1251 1319 1335 1487 1571 1731 Size: 68 16 152 84 160 Fragments arranged by size: 586 247 160 152 84 84 84 84 82 68 68 16 16 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 414 1731 Size: 414 1317 BsaHI Gr'CG_yC Cuts at: 0 650 1731 Size: 650 1081 BsaWI w'CCGG_w Cuts at: 0 1283 1731 Size: 1283 448 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 302 332 1731 Size: 302 30 1399 Fragments arranged by size: 1399 302 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 834 1338 1506 1590 1674 1731 Size: 834 504 168 84 84 57 Fragments arranged by size: 834 504 168 84 84 57 BsiEI CG_ry'CG Cuts at: 0 68 1731 Size: 68 1663 BsmI GAATG_Cn' Cuts at: 0 366 901 1153 1731 Size: 366 535 252 578 Fragments arranged by size: 578 535 366 252 Bsp1286I G_dGCh'C Cuts at: 0 985 1731 Size: 985 746 BspHI T'CATG_A Cuts at: 0 1227 1731 Size: 1227 504 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 604 1731 Size: 604 1127 BsrDI GCAATG_nn' Cuts at: 0 906 1242 1731 Size: 906 336 489 Fragments arranged by size: 906 489 336 BstYI r'GATC_y Cuts at: 0 180 1731 Size: 180 1551 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1099 1731 Size: 1099 632 BtsI GCAGTG_nn' Cuts at: 0 542 1494 1578 1731 Size: 542 952 84 153 Fragments arranged by size: 952 542 153 84 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 888 923 1224 1259 1731 Size: 888 35 301 35 472 Fragments arranged by size: 888 472 301 35 35 EaeI y'GGCC_r Cuts at: 0 152 623 1731 Size: 152 471 1108 Fragments arranged by size: 1108 471 152 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 34 1731 Size: 34 1697 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 247 329 915 983 1067 1151 1235 1251 Size: 247 82 586 68 84 84 84 16 Cuts at: 1251 1319 1335 1341 1487 1571 1731 Size: 68 16 6 146 84 160 Fragments arranged by size: 586 247 160 146 84 84 84 84 82 68 68 16 16 6 EcoNI CCTnn'n_nnAGG Cuts at: 0 190 1731 Size: 190 1541 EcoO109I rG'GnC_Cy Cuts at: 0 78 1731 Size: 78 1653 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 532 564 836 868 1731 Size: 532 32 272 32 863 Fragments arranged by size: 863 532 272 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 560 1124 1460 1731 Size: 560 564 336 271 Fragments arranged by size: 564 560 336 271 MscI TGG'CCA Cuts at: 0 625 1731 Size: 625 1106 MslI CAynn'nnrTG Cuts at: 0 455 1731 Size: 455 1276 NspI r_CATG'y Cuts at: 0 458 836 1731 Size: 458 378 895 Fragments arranged by size: 895 458 378 PasI CC'CwG_GG Cuts at: 0 252 1731 Size: 252 1479 PciI A'CATG_T Cuts at: 0 454 1731 Size: 454 1277 PpuMI rG'GwC_Cy Cuts at: 0 78 1731 Size: 78 1653 PstI C_TGCA'G Cuts at: 0 601 1731 Size: 601 1130 SanDI GG'GwC_CC Cuts at: 0 78 1731 Size: 78 1653 SbfI CC_TGCA'GG Cuts at: 0 601 1731 Size: 601 1130 SfcI C'TryA_G Cuts at: 0 101 597 1731 Size: 101 496 1134 Fragments arranged by size: 1134 496 101 SmaI CCC'GGG Cuts at: 0 17 1731 Size: 17 1714 SmlI C'TyrA_G Cuts at: 0 393 1441 1731 Size: 393 1048 290 Fragments arranged by size: 1048 393 290 StuI AGG'CCT Cuts at: 0 888 972 1056 1140 1392 1560 1644 1731 Size: 888 84 84 84 252 168 84 87 Fragments arranged by size: 888 252 168 87 84 84 84 84 StyI C'CwwG_G Cuts at: 0 423 1731 Size: 423 1308 TatI w'GTAC_w Cuts at: 0 158 209 1731 Size: 158 51 1522 Fragments arranged by size: 1522 158 51 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 302 334 1731 Size: 302 32 1397 Fragments arranged by size: 1397 302 32 XmaI C'CCGG_G Cuts at: 0 15 1731 Size: 15 1716 XmnI GAAnn'nnTTC Cuts at: 0 616 1731 Size: 616 1115 Enzymes that do cut: AcuI AflII AflIII AleI ApoI AvaI BanI Bme1580I BmrI BpmI BpuEI BsaHI BsaWI BsaXI BsgI BsiEI BsmI Bsp1286I BspHI BspMI BsrDI BstYI BtgZI BtsI CspCI EaeI EciI Eco57MI EcoNI EcoO109I Hin4I MmeI MscI MslI NspI PasI PciI PpuMI PstI SanDI SbfI SfcI SmaI SmlI StuI StyI TatI TstI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AgeI AhdI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI Bpu10I BsaI BsaAI BsaBI BseRI BseYI BsiHKAI BsiWI BsmBI BspEI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI ClaI DraI DraIII DrdI EagI EarI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SapI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TaqII Tth111I XbaI XcmI XhoI ZraI