Restriction map for
pFN21AE1148
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AviII BamHI BanI
BbeI BbrPI BbsI BciVI BclI
BfrI BglII BpuAI BsaBI BsaWI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstYI BstZ17I ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HinfI HpaI KasI KpnI MamI
MfeI MflI MluI MroI MseI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SbfI ScaI SexAI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tru9I Tsp509I Tth111I Van91I
XbaI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AcyI 1007 GACGCC G[GA]^CG[CT]C
Alw44I 859 GTGCAC G^TGCAC
AlwI 1281 GATCC GGATC(4/5)
AocI 165 CCTGAGG CC^T[ACGT]AGG
Aor51HI 150 AGCGCT AGC^GCT
ApaLI 859 GTGCAC G^TGCAC
Asp700I 471 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 736 CCTAGG C^CTAGG
BalI 50 TGGCCA TGG^CCA
BcgI 25 CGAGAACTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 737 CTAG C^TAG
BlnI 736 CCTAGG C^CTAGG
BsaAI 1198 TACGTG [CT]AC^GT[GA]
BsaHI 1007 GACGCC G[GA]^CG[CT]C
BseRI 1106 GAGGAG GAGGAG(10/8)
BsmBI 1059 CGTCTC CGTCTC(1/5)
BsmI 688 GAATGC GAATGC(1/-1)
BspMI 140 ACCTGC ACCTGC(4/8)
BsrDI 423 CATTGC GCAATG(2/0)
BsrFI 463 GCCGGT [GA]^CCGG[CT]
BstAPI 135 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1355 CCATCGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 165 CCTGAGG CC^T[ACGT]AGG
Cfr10I 463 GCCGGT [GA]^CCGG[CT]
DrdI 1360 GACCTGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 50 TGGCCA [CT]^GGCC[GA]
EarI 1378 GAAGAG CTCTTC(1/4)
Eco47III 150 AGCGCT AGC^GCT
Eco81I 165 CCTGAGG CC^T[ACGT]AGG
EcoNI 698 CCTGTGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I 1007 GACGCC G[GA]^CG[CT]C
Ksp632I 1378 GAAGAG CTCTTC(1/4)
KspI 247 CCGCGG CCGC^GG
MaeI 737 CTAG C^TAG
MluNI 50 TGGCCA TGG^CCA
MscI 50 TGGCCA TGG^CCA
MslI 694 CACACCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 344 CCATGG C^CATGG
NspI 640 ACATGC [GA]CATG^[CT]
SacII 247 CCGCGG CCGC^GG
SapI 1378 GAAGAGC GCTCTTC(1/4)
SfaNI 482 GCATC GCATC(5/9)
SfiI 1113 GGCCCTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 265 CTCAAG C^T[CT][GA]AG
TaqI 1358 TCGA T^CGA
TthHB8I 1358 TCGA T^CGA
XcmI 56 CCAGCCTCGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 471 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AvaI 848 CTCGGG C^[CT]CG[GA]G
983 CCCGAG
BlpI 146 GCTCAGC GC^T[ACGT]AGC
511 GCTCAGC
Bpu1102I 146 GCTCAGC GC^T[ACGT]AGC
511 GCTCAGC
BsaI 349 GAGACC GGTCTC(1/5)
787 GAGACC
BsgI 330 CTGCAC GTGCAG(16/14)
1066 CTGCAC
BsoBI 848 CTCGGG C^[CT]CG[GA]G
983 CCCGAG
BssSI 23 CACGAG CACGAG(-5/-1)
377 CTCGTG
CelII 146 GCTCAGC GC^T[ACGT]AGC
511 GCTCAGC
DpnII 366 GATC ^GATC
1281 GATC
EcoT14I 344 CCATGG C^C[AT][AT]GG
736 CCTAGG
MaeIII 16 GTGAC ^GT[ACGT]AC
637 GTGAC
MboI 366 GATC ^GATC
1281 GATC
NdeII 366 GATC ^GATC
1281 GATC
PshAI 270 GACCCACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1213 GACCTTCGTC
PvuII 514 CAGCTG CAG^CTG
778 CAGCTG
Sau3AI 366 GATC ^GATC
1281 GATC
StuI 733 AGGCCT AGG^CCT
810 AGGCCT
StyI 344 CCATGG C^C[AT][AT]GG
736 CCTAGG
Tsp45I 16 GTGAC ^GT[GC]AC
637 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
ApaI AspHI AvaII BanII BbvCI
BbvI BcnI BglI BmyI BpmI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DraII DsaI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HpaII
HphI ItaI MaeII MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIII NlaIV PpuMI PstI
RsaI Sau96I ScrFI SfcI TseI
TspRI