(Circular) (Six-base) MAPSORT of: pFN21AE1148.seq Check: 3082 from: 1 to: 6091 pFN21AE1148 6091 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2154 2154 Size: 6091 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3901 278 Size: 53 83 186 936 2365 2468 Fragments arranged by size: 2468 2365 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3434 1937 Size: 1497 4594 Acc65I G'GTAC_C Cuts at: 3412 3412 Size: 6091 AclI AA'CG_TT Cuts at: 1563 4218 4591 1563 Size: 2655 373 3063 Fragments arranged by size: 3063 2655 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2467 4154 5538 5806 1632 Size: 835 1687 1384 268 1917 Fragments arranged by size: 1917 1687 1384 835 268 AfeI AGC'GCT Cuts at: 2157 2157 Size: 6091 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5894 AflIII A'CryG_T Cuts at: 1203 4990 5967 1203 Size: 3787 977 1327 Fragments arranged by size: 3787 1327 977 AgeI A'CCGG_T Cuts at: 4922 4922 Size: 6091 AhdI GACnn_n'nnGTC Cuts at: 4820 4820 Size: 6091 AleI CACnn'nnGTG Cuts at: 1166 2703 1166 Size: 1537 4554 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2280 2594 1184 Size: 1096 314 4681 Fragments arranged by size: 4681 1096 314 AlwNI CAG_nnn'CTG Cuts at: 2146 2842 2914 2971 3260 3834 5406 2146 Size: 696 72 57 289 574 1572 2831 Fragments arranged by size: 2831 1572 696 574 289 72 57 ApaI G_GGCC'C Cuts at: 2013 2206 2252 2534 3034 3160 3184 2013 Size: 193 46 282 500 126 24 4920 Fragments arranged by size: 4920 500 282 193 126 46 24 ApaLI G'TGCA_C Cuts at: 2864 4148 5304 2864 Size: 1284 1156 3651 Fragments arranged by size: 3651 1284 1156 ApoI r'AATT_y Cuts at: 1493 3400 3626 3778 5887 1493 Size: 1907 226 152 2109 1697 Fragments arranged by size: 2109 1907 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4645 160 Size: 872 3613 1606 Fragments arranged by size: 3613 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6091 AvaI C'yCGr_G Cuts at: 1870 1943 2853 2988 3416 1870 Size: 73 910 135 428 4545 Fragments arranged by size: 4545 910 428 135 73 AvrII C'CTAG_G Cuts at: 2741 2741 Size: 6091 BamHI G'GATC_C Cuts at: 3421 3788 3421 Size: 367 5724 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3412 4867 618 Size: 325 217 627 1625 1455 1842 Fragments arranged by size: 1842 1625 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2013 2206 2252 2534 2751 3034 3160 3184 Size: 622 193 46 282 217 283 126 24 Cuts at: 3184 3410 1391 Size: 226 4072 Fragments arranged by size: 4072 622 283 282 226 217 193 126 46 24 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5120 BbvCI CC'TCA_GC Cuts at: 2462 2976 3162 2462 Size: 514 186 5391 Fragments arranged by size: 5391 514 186 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2019 2053 4282 4316 1903 Size: 34 82 34 2229 34 3678 Fragments arranged by size: 3678 2229 82 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3983 5193 3983 Size: 1210 4881 BclI T'GATC_A Cuts at: 1548 1548 Size: 6091 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2764 2838 3125 Size: 107 122 71 631 605 1092 74 287 Cuts at: 3125 3155 4702 136 Size: 30 1547 1525 Fragments arranged by size: 1547 1525 1092 631 605 287 122 107 74 71 30 BglII A'GATC_T Cuts at: 6086 6086 Size: 6091 BlpI GC'TnA_GC Cuts at: 2152 2517 3508 2152 Size: 365 991 4735 Fragments arranged by size: 4735 991 365 Bme1580I G_kGCm'C Cuts at: 1790 2013 2124 2206 2252 2534 2868 3034 3160 Size: 223 111 82 46 282 334 166 126 Cuts at: 3160 3184 4152 5308 1790 Size: 24 968 1156 2573 Fragments arranged by size: 2573 1156 968 334 282 223 166 126 111 82 46 24 BmgBI CAC'GTC Cuts at: 1318 2281 1318 Size: 963 5128 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3029 3187 3822 Size: 416 53 83 399 24 1612 158 635 Cuts at: 3822 4780 442 Size: 958 1753 Fragments arranged by size: 1753 1612 958 635 416 399 158 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6091 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2207 2429 2948 4751 1239 Size: 968 222 519 1803 2579 Fragments arranged by size: 2579 1803 968 519 222 Bpu10I CC'TnA_GC Cuts at: 2462 2976 3162 2462 Size: 514 186 5391 Fragments arranged by size: 5391 514 186 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2255 4216 5081 5379 2255 Size: 1961 865 298 2967 Fragments arranged by size: 2967 1961 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2348 2786 4754 882 Size: 617 849 438 1968 2219 Fragments arranged by size: 2219 1968 849 617 438 BsaAI yAC'GTr Cuts at: 493 1204 3205 3882 493 Size: 711 2001 677 2702 Fragments arranged by size: 2702 2001 711 677 BsaBI GATnn'nnATC Cuts at: 6085 6085 Size: 6091 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3013 3898 4280 4915 Size: 53 83 186 936 1480 885 382 635 Cuts at: 4915 275 Size: 1451 Fragments arranged by size: 1480 1451 936 885 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4524 4922 5196 5343 5754 4524 Size: 398 274 147 411 4861 Fragments arranged by size: 4861 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2180 2210 2738 2768 1178 Size: 30 165 30 777 30 528 30 4501 Fragments arranged by size: 4501 777 528 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3126 1186 Size: 1940 4151 BseYI C'CCAG_C Cuts at: 2212 2527 2533 2845 2982 3153 3235 3253 3263 Size: 315 6 312 137 171 82 18 10 Cuts at: 3263 5294 5790 2212 Size: 2031 496 2513 Fragments arranged by size: 2513 2031 496 315 312 171 137 82 18 10 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2320 3056 1163 Size: 573 584 736 4198 Fragments arranged by size: 4198 736 584 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3541 4302 5330 1540 Size: 275 186 1540 761 1028 2301 Fragments arranged by size: 2301 1540 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2518 2616 2688 2868 3042 4152 4237 5308 2518 Size: 98 72 180 174 1110 85 1071 3301 Fragments arranged by size: 3301 1110 1071 180 174 98 85 72 BsmI GAATG_Cn' Cuts at: 2699 3602 3695 2699 Size: 903 93 5095 Fragments arranged by size: 5095 903 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3070 3070 Size: 6091 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2124 2206 2252 2518 2534 2616 Size: 399 223 111 82 46 266 16 82 Cuts at: 2616 2688 2751 2868 3034 3042 3160 3184 3410 Size: 72 63 117 166 8 118 24 226 Cuts at: 3410 4152 4237 5308 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 266 226 223 166 118 117 111 85 82 82 72 63 46 24 16 8 BspEI T'CCGG_A Cuts at: 5754 5754 Size: 6091 BspHI T'CATG_A Cuts at: 3980 5710 3980 Size: 1730 4361 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2154 3446 844 Size: 1310 1292 3489 Fragments arranged by size: 3489 1310 1292 BsrBI CCG'CTC Cuts at: 1394 1823 3978 1394 Size: 429 2155 3507 Fragments arranged by size: 3507 2155 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2427 4586 4760 65 Size: 1183 1179 2159 174 1396 Fragments arranged by size: 2159 1396 1183 1179 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2468 4735 4922 1700 Size: 110 658 2267 187 2869 Fragments arranged by size: 2869 2267 658 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6091 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6091 BssSI C'ACGA_G Cuts at: 2028 2382 4151 5163 2028 Size: 354 1769 1012 2956 Fragments arranged by size: 2956 1769 1012 354 BstAPI GCAn_nnn'nTGC Cuts at: 794 2146 794 Size: 1352 4739 BstBI TT'CG_AA Cuts at: 4895 4895 Size: 6091 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6091 BstXI CCAn_nnnn'nTGG Cuts at: 3367 3367 Size: 6091 BstYI r'GATC_y Cuts at: 1972 3421 3788 4173 4190 5642 5728 5740 6086 Size: 1449 367 385 17 1452 86 12 346 Cuts at: 6086 1972 Size: 1977 Fragments arranged by size: 1977 1452 1449 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2171 2171 Size: 6091 BtgI C'CryG_G Cuts at: 513 1065 2004 2252 2349 2762 3352 513 Size: 552 939 248 97 413 590 3252 Fragments arranged by size: 3252 939 590 552 413 248 97 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5352 Fragments arranged by size: 5352 683 56 BtsI GCAGTG_nn' Cuts at: 3097 3607 4401 4421 3097 Size: 510 794 20 4767 Fragments arranged by size: 4767 794 510 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6091 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6056 DraI TTT'AAA Cuts at: 3395 3395 Size: 6091 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3371 5098 809 Size: 507 2055 1727 1802 Fragments arranged by size: 2055 1802 1727 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2055 3538 4427 Size: 54 1425 50 275 18 225 1483 889 Cuts at: 4427 6056 8 Size: 1629 43 Fragments arranged by size: 1629 1483 1425 889 275 225 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3538 1537 Size: 275 1726 4090 Fragments arranged by size: 4090 1726 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3378 4021 1353 Size: 2025 643 3423 Fragments arranged by size: 3423 2025 643 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4678 5052 5198 387 Size: 1064 138 3089 374 146 1280 Fragments arranged by size: 3089 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2207 2429 2467 2948 4154 4751 5538 Size: 393 575 222 38 481 1206 597 787 Cuts at: 5538 5806 1239 Size: 268 1524 Fragments arranged by size: 1524 1206 787 597 575 481 393 268 222 38 EcoNI CCTnn'n_nnAGG Cuts at: 2707 2707 Size: 6091 EcoO109I rG'GnC_Cy Cuts at: 2009 2182 2203 2248 2839 2849 2887 3030 3031 Size: 173 21 45 591 10 38 143 1 Cuts at: 3031 3118 3157 3267 3275 3298 2009 Size: 87 39 110 8 23 4802 Fragments arranged by size: 4802 591 173 143 110 87 45 39 38 23 21 10 8 1 EcoRI G'AATT_C Cuts at: 3400 3778 3400 Size: 378 5713 FspI TGC'GCA Cuts at: 4597 4597 Size: 6091 HaeII r_GCGC'y Cuts at: 1678 2159 2937 3010 3190 3334 5238 1678 Size: 481 778 73 180 144 1904 2531 Fragments arranged by size: 2531 1904 778 481 180 144 73 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4732 4764 4806 4838 1373 Size: 32 135 32 3160 32 42 32 2626 Fragments arranged by size: 3160 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3435 3681 669 Size: 988 281 1497 246 3079 Fragments arranged by size: 3079 1497 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6091 HpaI GTT'AAC Cuts at: 3681 3681 Size: 6091 KpnI G_GTAC'C Cuts at: 3416 3416 Size: 6091 MfeI C'AATT_G Cuts at: 3690 3690 Size: 6091 MluI A'CGCG_T Cuts at: 5967 5967 Size: 6091 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5205 5389 1305 Size: 3900 184 2007 Fragments arranged by size: 3900 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2057 10 Size: 54 1425 343 225 4044 Fragments arranged by size: 4044 1425 343 225 54 MslI CAynn'nnrTG Cuts at: 518 1166 2703 4049 4408 4567 518 Size: 648 1537 1346 359 159 2042 Fragments arranged by size: 2042 1537 1346 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2179 2254 2521 2785 2878 3153 3507 4184 Size: 573 75 267 264 93 275 354 677 Cuts at: 4184 5332 5577 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 573 354 275 267 264 245 93 75 NcoI C'CATG_G Cuts at: 513 1065 2004 2349 513 Size: 552 939 345 4255 Fragments arranged by size: 4255 939 552 345 NdeI CA'TA_TG Cuts at: 387 387 Size: 6091 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6091 NotI GC'GGCC_GC Cuts at: 3538 3538 Size: 6091 NspI r_CATG'y Cuts at: 1132 1229 2649 3449 4994 1132 Size: 97 1420 800 1545 2229 Fragments arranged by size: 2229 1545 1420 800 97 PasI CC'CwG_GG Cuts at: 1348 3130 1348 Size: 1782 4309 PciI A'CATG_T Cuts at: 4990 4990 Size: 6091 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6091 PfoI T'CCnGG_A Cuts at: 2070 2616 2070 Size: 546 5545 PmeI GTTT'AAAC Cuts at: 3395 3395 Size: 6091 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4160 4192 5673 5705 4160 Size: 32 1481 32 4546 Fragments arranged by size: 4546 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2182 2849 2887 3267 2182 Size: 667 38 380 5006 Fragments arranged by size: 5006 667 380 38 PshAI GACnn'nnGTC Cuts at: 1613 2279 3222 1613 Size: 666 943 4482 Fragments arranged by size: 4482 943 666 PsiI TTA'TAA Cuts at: 3661 3661 Size: 6091 PspOMI G'GGCC_C Cuts at: 2009 2202 2248 2530 3030 3156 3180 2009 Size: 193 46 282 500 126 24 4920 Fragments arranged by size: 4920 500 282 193 126 46 24 PstI C_TGCA'G Cuts at: 830 2587 2811 2933 3443 830 Size: 1757 224 122 510 3478 Fragments arranged by size: 3478 1757 510 224 122 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6091 PvuII CAG'CTG Cuts at: 2521 2785 2521 Size: 264 5827 SacII CC_GC'GG Cuts at: 2255 2255 Size: 6091 SalI G'TCGA_C Cuts at: 1936 3433 1936 Size: 1497 4594 SanDI GG'GwC_CC Cuts at: 2887 3267 2887 Size: 380 5711 SapI GCTCTTCn'nnn_ Cuts at: 3378 3378 Size: 6091 SbfI CC_TGCA'GG Cuts at: 3443 3443 Size: 6091 ScaI AGT'ACT Cuts at: 4339 6051 4339 Size: 1712 4379 SfcI C'TryA_G Cuts at: 826 1045 2583 2807 2929 3439 4574 5255 5446 Size: 219 1538 224 122 510 1135 681 191 Cuts at: 5446 826 Size: 1471 Fragments arranged by size: 1538 1471 1135 681 510 224 219 191 122 SfiI GGCCn_nnn'nGGCC Cuts at: 3125 3125 Size: 6091 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6091 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6091 SmaI CCC'GGG Cuts at: 1872 3418 1872 Size: 1546 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2270 4195 5096 5358 820 Size: 197 926 327 1925 901 262 1553 Fragments arranged by size: 1925 1553 926 901 327 262 197 SnaBI TAC'GTA Cuts at: 493 3882 493 Size: 3389 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6091 SphI G_CATG'C Cuts at: 1132 3449 1132 Size: 2317 3774 SspI AAT'ATT Cuts at: 5 52 4015 5 Size: 47 3963 2081 Fragments arranged by size: 3963 2081 47 StuI AGG'CCT Cuts at: 1517 2740 2817 1517 Size: 1223 77 4791 Fragments arranged by size: 4791 1223 77 StyI C'CwwG_G Cuts at: 513 1065 2004 2349 2741 3530 513 Size: 552 939 345 392 789 3074 Fragments arranged by size: 3074 939 789 552 392 345 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2111 2883 3062 3340 4118 4135 4288 Size: 1255 531 772 179 278 778 17 153 Cuts at: 4288 4473 4918 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 778 772 531 445 278 185 179 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2493 4337 6049 Size: 274 80 33 51 1443 516 1844 1712 Cuts at: 6049 96 Size: 138 Fragments arranged by size: 1844 1712 1443 516 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6051 6083 6051 Size: 32 6059 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6091 XbaI T'CTAG_A Cuts at: 3427 3427 Size: 6091 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2068 2068 Size: 6091 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6091 XmaI C'CCGG_G Cuts at: 1870 3416 1870 Size: 1546 4545 XmnI GAAnn'nnTTC Cuts at: 2480 4220 2480 Size: 1740 4351 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3899 276 Size: 53 83 186 936 2365 2468 Fragments arranged by size: 2468 2365 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BbeI BfrBI BplI BsiWI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SexAI SfoI SrfI SwaI