(Linear) (Six-base) MAPSORT of: KIEE1148.seq Check: 4866 from: 1 to: 1386 KIEE1148.orf With 182 enzymes: SgfI * July 15, 2010 18:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 149 1386 Size: 149 1237 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 462 1386 Size: 462 924 AfeI AGC'GCT Cuts at: 0 152 1386 Size: 152 1234 AleI CACnn'nnGTG Cuts at: 0 698 1386 Size: 698 688 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 275 589 1386 Size: 275 314 797 Fragments arranged by size: 797 314 275 AlwNI CAG_nnn'CTG Cuts at: 0 141 837 909 966 1255 1386 Size: 141 696 72 57 289 131 Fragments arranged by size: 696 289 141 131 72 57 ApaI G_GGCC'C Cuts at: 0 8 201 247 529 1029 1155 1179 1386 Size: 8 193 46 282 500 126 24 207 Fragments arranged by size: 500 282 207 193 126 46 24 8 ApaLI G'TGCA_C Cuts at: 0 859 1386 Size: 859 527 AvaI C'yCGr_G Cuts at: 0 848 983 1386 Size: 848 135 403 Fragments arranged by size: 848 403 135 AvrII C'CTAG_G Cuts at: 0 736 1386 Size: 736 650 BanII G_rGCy'C Cuts at: 0 8 201 247 529 746 1029 1155 1179 Size: 8 193 46 282 217 283 126 24 Cuts at: 1179 1386 Size: 207 Fragments arranged by size: 283 282 217 207 193 126 46 24 8 BbvCI CC'TCA_GC Cuts at: 0 457 971 1157 1386 Size: 457 514 186 229 Fragments arranged by size: 514 457 229 186 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 14 48 1386 Size: 14 34 1338 Fragments arranged by size: 1338 34 14 BglI GCCn_nnn'nGGC Cuts at: 0 759 833 1120 1150 1386 Size: 759 74 287 30 236 Fragments arranged by size: 759 287 236 74 30 BlpI GC'TnA_GC Cuts at: 0 147 512 1386 Size: 147 365 874 Fragments arranged by size: 874 365 147 Bme1580I G_kGCm'C Cuts at: 0 8 119 201 247 529 863 1029 1155 Size: 8 111 82 46 282 334 166 126 Cuts at: 1155 1179 1386 Size: 24 207 Fragments arranged by size: 334 282 207 166 126 111 82 46 24 8 BmgBI CAC'GTC Cuts at: 0 276 1386 Size: 276 1110 BmrI ACTGGGnnnn_n' Cuts at: 0 1024 1182 1386 Size: 1024 158 204 Fragments arranged by size: 1024 204 158 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 202 424 943 1386 Size: 202 222 519 443 Fragments arranged by size: 519 443 222 202 Bpu10I CC'TnA_GC Cuts at: 0 457 971 1157 1386 Size: 457 514 186 229 Fragments arranged by size: 514 457 229 186 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 250 1386 Size: 250 1136 BsaI GGTCTCn'nnnn_ Cuts at: 0 343 781 1386 Size: 343 438 605 Fragments arranged by size: 605 438 343 BsaAI yAC'GTr Cuts at: 0 1200 1386 Size: 1200 186 BsaHI Gr'CG_yC Cuts at: 0 1008 1386 Size: 1008 378 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 175 205 733 763 1386 Size: 175 30 528 30 623 Fragments arranged by size: 623 528 175 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1121 1386 Size: 1121 265 BseYI C'CCAG_C Cuts at: 0 207 522 528 840 977 1148 1230 1248 Size: 207 315 6 312 137 171 82 18 Cuts at: 1248 1258 1386 Size: 10 128 Fragments arranged by size: 315 312 207 171 137 128 82 18 10 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 315 1051 1386 Size: 315 736 335 Fragments arranged by size: 736 335 315 BsiHKAI G_wGCw'C Cuts at: 0 513 611 683 863 1037 1386 Size: 513 98 72 180 174 349 Fragments arranged by size: 513 349 180 174 98 72 BsmI GAATG_Cn' Cuts at: 0 694 1386 Size: 694 692 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1065 1386 Size: 1065 321 Bsp1286I G_dGCh'C Cuts at: 0 8 119 201 247 513 529 611 683 Size: 8 111 82 46 266 16 82 72 Cuts at: 683 746 863 1029 1037 1155 1179 1386 Size: 63 117 166 8 118 24 207 Fragments arranged by size: 266 207 166 118 117 111 82 82 72 63 46 24 16 8 8 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 149 1386 Size: 149 1237 BsrDI GCAATG_nn' Cuts at: 0 422 1386 Size: 422 964 BsrFI r'CCGG_y Cuts at: 0 463 1386 Size: 463 923 BssSI C'ACGA_G Cuts at: 0 23 377 1386 Size: 23 354 1009 Fragments arranged by size: 1009 354 23 BstAPI GCAn_nnn'nTGC Cuts at: 0 141 1386 Size: 141 1245 BstXI CCAn_nnnn'nTGG Cuts at: 0 1362 1386 Size: 1362 24 Bsu36I CC'TnA_GG Cuts at: 0 166 1386 Size: 166 1220 BtgI C'CryG_G Cuts at: 0 247 344 757 1347 1386 Size: 247 97 413 590 39 Fragments arranged by size: 590 413 247 97 39 BtsI GCAGTG_nn' Cuts at: 0 1092 1386 Size: 1092 294 DrdI GACnn_nn'nnGTC Cuts at: 0 1366 1386 Size: 1366 20 EaeI y'GGCC_r Cuts at: 0 50 1386 Size: 50 1336 EarI CTCTTCn'nnn_ Cuts at: 0 1373 1386 Size: 1373 13 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 202 424 462 943 1386 Size: 202 222 38 481 443 Fragments arranged by size: 481 443 222 202 38 EcoNI CCTnn'n_nnAGG Cuts at: 0 702 1386 Size: 702 684 EcoO109I rG'GnC_Cy Cuts at: 0 4 177 198 243 834 844 882 1025 Size: 4 173 21 45 591 10 38 143 Cuts at: 1025 1026 1113 1152 1262 1270 1293 1386 Size: 1 87 39 110 8 23 93 Fragments arranged by size: 591 173 143 110 93 87 45 39 38 23 21 10 8 4 1 HaeII r_GCGC'y Cuts at: 0 154 932 1005 1185 1329 1386 Size: 154 778 73 180 144 57 Fragments arranged by size: 778 180 154 144 73 57 MscI TGG'CCA Cuts at: 0 52 1386 Size: 52 1334 MslI CAynn'nnrTG Cuts at: 0 698 1386 Size: 698 688 MspA1I CmG'CkG Cuts at: 0 174 249 516 780 873 1148 1386 Size: 174 75 267 264 93 275 238 Fragments arranged by size: 275 267 264 238 174 93 75 NcoI C'CATG_G Cuts at: 0 344 1386 Size: 344 1042 NspI r_CATG'y Cuts at: 0 644 1386 Size: 644 742 PasI CC'CwG_GG Cuts at: 0 1125 1386 Size: 1125 261 PfoI T'CCnGG_A Cuts at: 0 65 611 1386 Size: 65 546 775 Fragments arranged by size: 775 546 65 PpuMI rG'GwC_Cy Cuts at: 0 177 844 882 1262 1386 Size: 177 667 38 380 124 Fragments arranged by size: 667 380 177 124 38 PshAI GACnn'nnGTC Cuts at: 0 274 1217 1386 Size: 274 943 169 Fragments arranged by size: 943 274 169 PspOMI G'GGCC_C Cuts at: 0 4 197 243 525 1025 1151 1175 1386 Size: 4 193 46 282 500 126 24 211 Fragments arranged by size: 500 282 211 193 126 46 24 4 PstI C_TGCA'G Cuts at: 0 582 806 928 1386 Size: 582 224 122 458 Fragments arranged by size: 582 458 224 122 PvuII CAG'CTG Cuts at: 0 516 780 1386 Size: 516 264 606 Fragments arranged by size: 606 516 264 SacII CC_GC'GG Cuts at: 0 250 1386 Size: 250 1136 SanDI GG'GwC_CC Cuts at: 0 882 1262 1386 Size: 882 380 124 Fragments arranged by size: 882 380 124 SapI GCTCTTCn'nnn_ Cuts at: 0 1373 1386 Size: 1373 13 SfcI C'TryA_G Cuts at: 0 578 802 924 1386 Size: 578 224 122 462 Fragments arranged by size: 578 462 224 122 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1120 1386 Size: 1120 266 SmlI C'TyrA_G Cuts at: 0 265 1386 Size: 265 1121 StuI AGG'CCT Cuts at: 0 735 812 1386 Size: 735 77 574 Fragments arranged by size: 735 574 77 StyI C'CwwG_G Cuts at: 0 344 736 1386 Size: 344 392 650 Fragments arranged by size: 650 392 344 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 106 878 1057 1335 1386 Size: 106 772 179 278 51 Fragments arranged by size: 772 278 179 106 51 TatI w'GTAC_w Cuts at: 0 488 1386 Size: 488 898 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 63 1386 Size: 63 1323 XmnI GAAnn'nnTTC Cuts at: 0 475 1386 Size: 475 911 Enzymes that do cut: AarI AcuI AfeI AleI AlfI AlwNI ApaI ApaLI AvaI AvrII BanII BbvCI BcgI BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI BsrFI BssSI BstAPI BstXI Bsu36I BtgI BtsI DrdI EaeI EarI Eco57MI EcoNI EcoO109I HaeII MscI MslI MspA1I NcoI NspI PasI PfoI PpuMI PshAI PspOMI PstI PvuII SacII SanDI SapI SfcI SfiI SmlI StuI StyI TaqII TatI XcmI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AloI ApoI AscI AseI AsiSI BaeI BamHI BanI BbeI BbsI BciVI BclI BfrBI BglII BmtI BplI BsaBI BsaWI BsiEI BsiWI BspEI BspHI BsrBI BsrGI BssHII BstBI BstEII BstYI BstZ17I BtgZI ClaI CspCI DraI DraIII EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PflMI PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII SacI SalI SbfI ScaI SexAI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI XhoI XmaI ZraI