################################## # NO CUT ENZYMES ( 162 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI Alw44I AlwNI AocI ApaI ApaLI AscI AseI AsnI Asp718I AspI AvaI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BclI BfrI BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsiYI BslI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FauI FbaI FseI FspI HaeIII HphI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MslI MspA1I MunI NaeI NarI NcoI NdeI NgoMIV NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI Sau96I SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccII 672 CGCG CG^CG AcsI 404 GAATTC [GA]^AATT[CT] AcyI 542 GGCGTC G[GA]^CG[CT]C AhdI 614 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 160 GATCC GGATC(4/5) Aor51HI 463 AGCGCT AGC^GCT ApoI 404 GAATTC [GA]^AATT[CT] Asp700I 400 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 614 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 266 GAGCTC G[AT]GC[AT]^C BanII 266 GAGCTC G[GA]GC[CT]^C BciVI 186 GTATCC GTATCC(6/5) BcnI 17 CCCGG CC^[GC]GG BglI 534 GCCGCTAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 453 AGATCT A^GATCT BmyI 266 GAGCTC G[AGT]GC[ACT]^C BsaHI 542 GGCGTC G[GA]^CG[CT]C BsgI 666 GTGCAG GTGCAG(16/14) BsiHKAI 266 GAGCTC G[AT]GC[AT]^C Bsp1286I 266 GAGCTC G[AGT]GC[ACT]^C BsrDI 506 CATTGC GCAATG(2/0) BsrI 143 ACTGG ACTGG(1/-1) BstF5I 41 GGATG GGATG(2/0) BstNI 190 CCAGG CC^[AT]GG BstUI 672 CGCG CG^CG Eam1105I 614 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 463 AGCGCT AGC^GCT EcoRI 404 GAATTC G^AATTC EcoRII 190 CCAGG ^CC[AT]GG FokI 41 GGATG GGATG(9/13) HaeII 463 AGCGCT [GA]GCGC^[CT] HapII 18 CCGG C^CGG HgaI 543 GCGTC GACGC(5/10) Hin1I 542 GGCGTC G[GA]^CG[CT]C HindIII 559 AAGCTT A^AGCTT HpaI 52 GTTAAC GTT^AAC HpaII 18 CCGG C^CGG MaeII 380 ACGT A^CGT MaeIII 622 GTCAC ^GT[ACGT]AC MspI 18 CCGG C^CGG MvaI 190 CCAGG CC^[AT]GG MvnI 672 CGCG CG^CG MwoI 534 GCCGCTAAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NciI 17 CCCGG CC^[GC]GG NheI 135 GCTAGC G^CTAGC NspI 174 GCATGC [GA]CATG^[CT] PshAI 652 GACAGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 266 GAGCTC GAGCT^C SapI 529 GAAGAGC GCTCTTC(1/4) SphI 174 GCATGC GCATG^C Tsp45I 622 GTCAC ^GT[GC]AC XmnI 400 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AciI 535 CCGC CCGC(-3/-1) 673 GCGG BbvI 63 GCAGC GCAGC(8/12) 668 GCAGC BsaJI 152 CCAAGG C^C[ACGT][ACGT]GG 163 CCTTGG BseRI 128 GAGGAG GAGGAG(10/8) 553 GAGGAG BssKI 17 CCCGG ^CC[ACGT]GG 190 CCAGG BstYI 159 AGATCC [GA]^GATC[CT] 453 AGATCT CfoI 464 GCGC GCG^C 671 GCGC DdeI 467 CTCAG C^T[ACGT]AG 538 CTAAG DpnII 160 GATC ^GATC 454 GATC EarI 211 GAAGAG CTCTTC(1/4) 529 GAAGAG EcoT14I 152 CCAAGG C^C[AT][AT]GG 163 CCTTGG HhaI 464 GCGC GCG^C 671 GCGC HinP1I 464 GCGC G^CGC 671 GCGC HincII 52 GTTAAC GT[CT]^[GA]AC 649 GTTGAC HindII 52 GTTAAC GT[CT]^[GA]AC 649 GTTGAC HinfI 311 GAATC G^A[ACGT]TC 495 GAATC Ksp632I 211 GAAGAG CTCTTC(1/4) 529 GAAGAG MboI 160 GATC ^GATC 454 GATC MflI 159 AGATCC [GA]^GATC[CT] 453 AGATCT NdeII 160 GATC ^GATC 454 GATC Sau3AI 160 GATC ^GATC 454 GATC ScrFI 17 CCCGG CC^[ACGT]GG 190 CCAGG SfcI 89 CTATAG C^T[GA][CT]AG 479 CTACAG SmlI 259 CTTGAG C^T[CT][GA]AG 436 CTTGAG StyI 152 CCAAGG C^C[AT][AT]GG 163 CCTTGG TfiI 311 GAATC G^A[AT]TC 495 GAATC TseI 63 GCAGC G^C[AT]GC 668 GCAGC Tsp509I 405 AATT ^AATT 491 AATT XhoII 159 AGATCC [GA]^GATC[CT] 453 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 15 enzymes) # ######################################### AfaI AluI BfaI BsmFI Cac8I Fnu4HI ItaI MaeI MboII MnlI MseI NlaIII RsaI Tru9I TspRI