(Circular) (Six-base) MAPSORT of: pFN21AE4027.seq Check: 9580 from: 1 to: 5413 pFN21AE4027 5413 bp With 182 enzymes: SgfI * March 28, 2012 13:11 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3223 278 Size: 53 83 186 936 1687 2468 Fragments arranged by size: 2468 1687 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2756 1937 Size: 819 4594 Acc65I G'GTAC_C Cuts at: 2734 2734 Size: 5413 AclI AA'CG_TT Cuts at: 1563 3540 3913 1563 Size: 1977 373 3063 Fragments arranged by size: 3063 1977 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2553 2667 3476 4860 5128 1632 Size: 921 114 809 1384 268 1917 Fragments arranged by size: 1917 1384 921 809 268 114 AfeI AGC'GCT Cuts at: 2470 2470 Size: 5413 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5216 AflIII A'CryG_T Cuts at: 1203 4312 5289 1203 Size: 3109 977 1327 Fragments arranged by size: 3109 1327 977 AgeI A'CCGG_T Cuts at: 4244 4244 Size: 5413 AhdI GACnn_n'nnGTC Cuts at: 2624 4142 2624 Size: 1518 3895 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5413 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2225 1184 Size: 1041 4372 AlwNI CAG_nnn'CTG Cuts at: 3156 4728 3156 Size: 1572 3841 ApaLI G'TGCA_C Cuts at: 3470 4626 3470 Size: 1156 4257 ApoI r'AATT_y Cuts at: 1493 2409 2722 2948 3100 5209 1493 Size: 916 313 226 152 2109 1697 Fragments arranged by size: 2109 1697 916 313 226 152 AseI AT'TA_AT Cuts at: 160 1032 3967 160 Size: 872 2935 1606 Fragments arranged by size: 2935 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5413 AvaI C'yCGr_G Cuts at: 1870 1943 2738 1870 Size: 73 795 4545 Fragments arranged by size: 4545 795 73 BamHI G'GATC_C Cuts at: 2743 3110 2743 Size: 367 5046 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2734 4189 618 Size: 325 217 627 947 1455 1842 Fragments arranged by size: 1842 1455 947 627 325 217 BanII G_rGCy'C Cuts at: 1391 2275 2732 1391 Size: 884 457 4072 Fragments arranged by size: 4072 884 457 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4442 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3604 3638 1903 Size: 34 1667 34 3678 Fragments arranged by size: 3678 1667 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2202 3305 4515 2202 Size: 1103 1210 3100 Fragments arranged by size: 3100 1210 1103 BclI T'GATC_A Cuts at: 1548 1548 Size: 5413 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2545 4024 136 Size: 107 122 71 631 605 873 1479 1525 Fragments arranged by size: 1525 1479 873 631 605 122 107 71 BglII A'GATC_T Cuts at: 2458 5408 2458 Size: 2950 2463 BlpI GC'TnA_GC Cuts at: 2830 2830 Size: 5413 Bme1580I G_kGCm'C Cuts at: 1790 3474 4630 1790 Size: 1684 1156 2573 Fragments arranged by size: 2573 1684 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5413 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3144 4102 442 Size: 416 53 83 399 24 1727 958 1753 Fragments arranged by size: 1753 1727 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2144 1056 Size: 1088 4325 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4073 1239 Size: 2834 2579 Bpu10I CC'TnA_GC Cuts at: 2543 2543 Size: 5413 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2285 2462 3538 4403 4701 2285 Size: 177 1076 865 298 2997 Fragments arranged by size: 2997 1076 865 298 177 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4076 882 Size: 617 2577 2219 Fragments arranged by size: 2577 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3204 493 Size: 711 2000 2702 Fragments arranged by size: 2702 2000 711 BsaBI GATnn'nnATC Cuts at: 5407 5407 Size: 5413 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2548 3220 3602 4237 Size: 53 83 186 936 1015 672 382 635 Cuts at: 4237 275 Size: 1451 Fragments arranged by size: 1451 1015 936 672 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3846 4244 4518 4665 5076 3846 Size: 398 274 147 411 4183 Fragments arranged by size: 4183 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2262 2292 1178 Size: 30 165 30 859 30 4299 Fragments arranged by size: 4299 859 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2148 2573 1186 Size: 962 425 4026 Fragments arranged by size: 4026 962 425 BseYI C'CCAG_C Cuts at: 4616 5112 4616 Size: 496 4917 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2692 1163 Size: 573 956 3884 Fragments arranged by size: 3884 956 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2863 3624 4652 1540 Size: 275 186 862 761 1028 2301 Fragments arranged by size: 2301 1028 862 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2275 3474 3559 4630 2275 Size: 1199 85 1071 3058 Fragments arranged by size: 3058 1199 1071 85 BsmI GAATG_Cn' Cuts at: 2924 3017 2924 Size: 93 5320 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2275 2732 3474 3559 4630 1391 Size: 399 485 457 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 485 457 399 85 BspEI T'CCGG_A Cuts at: 5076 5076 Size: 5413 BspHI T'CATG_A Cuts at: 3302 5032 3302 Size: 1730 3683 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2768 844 Size: 1924 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3300 1394 Size: 429 1477 3507 Fragments arranged by size: 3507 1477 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2510 3908 4082 65 Size: 1183 1262 1398 174 1396 Fragments arranged by size: 1398 1396 1262 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4057 4244 1700 Size: 110 2247 187 2869 Fragments arranged by size: 2869 2247 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5413 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5413 BssSI C'ACGA_G Cuts at: 3473 4485 3473 Size: 1012 4401 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5413 BstBI TT'CG_AA Cuts at: 4217 4217 Size: 5413 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5413 BstYI r'GATC_y Cuts at: 1972 2164 2458 2743 3110 3495 3512 4964 5050 Size: 192 294 285 367 385 17 1452 86 Cuts at: 5050 5062 5408 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 294 285 192 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4861 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4674 Fragments arranged by size: 4674 683 56 BtsI GCAGTG_nn' Cuts at: 2929 3723 3743 2929 Size: 794 20 4599 Fragments arranged by size: 4599 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5413 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5378 DraI TTT'AAA Cuts at: 2717 2717 Size: 5413 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4420 809 Size: 507 3104 1802 Fragments arranged by size: 3104 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2860 3749 5378 Size: 54 1425 50 275 18 1030 889 1629 Cuts at: 5378 8 Size: 43 Fragments arranged by size: 1629 1425 1030 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2860 1537 Size: 275 1048 4090 Fragments arranged by size: 4090 1048 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2211 2529 3343 1353 Size: 858 318 814 3423 Fragments arranged by size: 3423 858 814 318 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4000 4374 4520 387 Size: 1064 138 2411 374 146 1280 Fragments arranged by size: 2411 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2273 2273 Size: 5413 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2553 2667 3476 4073 4860 5128 1239 Size: 393 921 114 809 597 787 268 1524 Fragments arranged by size: 1524 921 809 787 597 393 268 114 EcoRI G'AATT_C Cuts at: 2409 2722 3100 2409 Size: 313 378 4722 Fragments arranged by size: 4722 378 313 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2087 2087 Size: 5413 FspI TGC'GCA Cuts at: 3919 3919 Size: 5413 HaeII r_GCGC'y Cuts at: 1678 2472 4560 1678 Size: 794 2088 2531 Fragments arranged by size: 2531 2088 794 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2610 2642 4054 4086 4128 Size: 32 135 32 1038 32 1412 32 42 Cuts at: 4128 4160 1373 Size: 32 2626 Fragments arranged by size: 2626 1412 1038 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2059 2656 2757 3003 669 Size: 988 281 121 597 101 246 3079 Fragments arranged by size: 3079 988 597 281 246 121 101 HindIII A'AGCT_T Cuts at: 748 2564 748 Size: 1816 3597 HpaI GTT'AAC Cuts at: 2059 3003 2059 Size: 944 4469 KpnI G_GTAC'C Cuts at: 2738 2738 Size: 5413 MfeI C'AATT_G Cuts at: 3012 3012 Size: 5413 MluI A'CGCG_T Cuts at: 5289 5289 Size: 5413 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4527 4711 1305 Size: 3222 184 2007 Fragments arranged by size: 3222 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3591 Fragments arranged by size: 3591 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3371 3730 3889 518 Size: 648 2205 359 159 2042 Fragments arranged by size: 2205 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2829 3506 4654 4899 1606 Size: 1223 677 1148 245 2120 Fragments arranged by size: 2120 1223 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4861 NdeI CA'TA_TG Cuts at: 387 387 Size: 5413 NheI G'CTAG_C Cuts at: 1052 2140 1052 Size: 1088 4325 NotI GC'GGCC_GC Cuts at: 2860 2860 Size: 5413 NspI r_CATG'y Cuts at: 1132 1229 2183 2771 4316 1132 Size: 97 954 588 1545 2229 Fragments arranged by size: 2229 1545 954 588 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5413 PciI A'CATG_T Cuts at: 4312 4312 Size: 5413 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5413 PmeI GTTT'AAAC Cuts at: 2717 2717 Size: 5413 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3482 3514 4995 5027 3482 Size: 32 1481 32 3868 Fragments arranged by size: 3868 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2661 1613 Size: 1048 4365 PsiI TTA'TAA Cuts at: 2983 2983 Size: 5413 PstI C_TGCA'G Cuts at: 830 2765 830 Size: 1935 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5413 SacI G_AGCT'C Cuts at: 2275 2275 Size: 5413 SalI G'TCGA_C Cuts at: 1936 2755 1936 Size: 819 4594 SapI GCTCTTCn'nnn_ Cuts at: 2529 2529 Size: 5413 SbfI CC_TGCA'GG Cuts at: 2765 2765 Size: 5413 ScaI AGT'ACT Cuts at: 3661 5373 3661 Size: 1712 3701 SfcI C'TryA_G Cuts at: 826 1045 2094 2484 2761 3896 4577 4768 826 Size: 219 1049 390 277 1135 681 191 1471 Fragments arranged by size: 1471 1135 1049 681 390 277 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5413 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5413 SmaI CCC'GGG Cuts at: 1872 2740 1872 Size: 868 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2264 2441 3517 4418 4680 820 Size: 197 926 321 177 1076 901 262 1553 Fragments arranged by size: 1553 1076 926 901 321 262 197 177 SnaBI TAC'GTA Cuts at: 493 3204 493 Size: 2711 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5413 SphI G_CATG'C Cuts at: 1132 2183 2771 1132 Size: 1051 588 3774 Fragments arranged by size: 3774 1051 588 SspI AAT'ATT Cuts at: 5 52 3337 5 Size: 47 3285 2081 Fragments arranged by size: 3285 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5413 StyI C'CwwG_G Cuts at: 513 1065 2157 2168 2852 513 Size: 552 1092 11 684 3074 Fragments arranged by size: 3074 1092 684 552 11 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3440 3457 3610 3795 4240 325 Size: 1255 1860 17 153 185 445 1498 Fragments arranged by size: 1860 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2049 2113 3659 Size: 274 80 33 51 1443 72 64 1546 Cuts at: 3659 5371 96 Size: 1712 138 Fragments arranged by size: 1712 1546 1443 274 138 80 72 64 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5373 5405 5373 Size: 32 5381 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5413 XbaI T'CTAG_A Cuts at: 2749 2749 Size: 5413 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5413 XmaI C'CCGG_G Cuts at: 1870 2738 1870 Size: 868 4545 XmnI GAAnn'nnTTC Cuts at: 2409 3542 2409 Size: 1133 4280 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3221 276 Size: 53 83 186 936 1687 2468 Fragments arranged by size: 2468 1687 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI