Restriction map for
pFN21AE9514
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII BalI BamHI
BbeI BbrPI BbvCI BcnI BfrI
BglI BglII BlpI Bpu1102I BsaAI
BsaHI BseAI BseRI BsiEI BsiWI
BsmBI BsoBI BspDI BspEI BspLU11I
BsrBI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraII EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FseI FspI
HaeII Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MfeI
MluI MluNI MroI MscI MseI
MslI MunI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tru9I Tth111I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccII 145 CGCG CG^CG
AciI 144 CCGC CCGC(-3/-1)
AgeI 136 ACCGGT A^CCGGT
AspHI 963 GAGCAC G[AT]GC[AT]^C
AvrII 577 CCTAGG C^CTAGG
BanI 386 GGCACC G^G[CT][GA]CC
BanII 823 GGGCTC G[GA]GC[CT]^C
BbsI 553 GTCTTC GAAGAC(2/6)
BcgI 842 GCAGCTCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 563 TGATCA T^GATCA
BlnI 577 CCTAGG C^CTAGG
BpmI 585 CTCCAG CTGGAG(16/14)
BpuAI 553 GTCTTC GAAGAC(2/6)
BsaBI 564 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 136 ACCGGT [AT]^CCGG[AT]
BsiHKAI 963 GAGCAC G[AT]GC[AT]^C
BsmFI 163 GTCCC GGGAC(10/14)
BsmI 121 GCATTC GAATGC(1/-1)
Bsp1407I 863 TGTACA T^GTACA
BspHI 1167 TCATGA T^CATGA
BsrDI 1065 CATTGC GCAATG(2/0)
BsrFI 136 ACCGGT [GA]^CCGG[CT]
BsrGI 863 TGTACA T^GTACA
BstAPI 1133 GCAGTATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 145 CGCG CG^CG
Cac8I 838 GCCAGC GC[ACGT]^[ACGT]GC
CfoI 53 GCGC GCG^C
Cfr10I 136 ACCGGT [GA]^CCGG[CT]
DraIII 755 CACTTAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 171 GACACAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 440 CCATGG C^C[GA][CT]GG
FbaI 563 TGATCA T^GATCA
HapII 137 CCGG C^CGG
HgaI 146 GCGTC GACGC(5/10)
HhaI 53 GCGC GCG^C
HinP1I 53 GCGC G^CGC
HpaII 137 CCGG C^CGG
MaeII 1004 ACGT A^CGT
MamI 564 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MnlI 543 CCTC CCTC(7/6)
MspA1I 175 CAGCTG C[AC]G^C[GT]G
MspI 137 CCGG C^CGG
MvnI 145 CGCG CG^CG
NcoI 440 CCATGG C^CATGG
NspI 659 ACATGC [GA]CATG^[CT]
PinAI 136 ACCGGT A^CCGGT
PvuII 175 CAGCTG CAG^CTG
RcaI 1167 TCATGA T^CATGA
SmlI 546 CTTGAG C^T[CT][GA]AG
SspBI 863 TGTACA T^GTACA
TaqI 1022 TCGA T^CGA
TthHB8I 1022 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 309 GAATTT [GA]^AATT[CT]
487 AAATTT
AfaI 864 GTAC GT^AC
883 GTAC
AlwI 365 GATCC GGATC(4/5)
1121 GATCC
AlwNI 1125 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
1134 CAGTATCTG
ApoI 309 GAATTT [GA]^AATT[CT]
487 AAATTT
AvaII 162 GGTCC G^G[AT]CC
270 GGACC
BfaI 578 CTAG C^TAG
1042 CTAG
BmyI 823 GGGCTC G[AGT]GC[ACT]^C
963 GAGCAC
Bsp1286I 823 GGGCTC G[AGT]GC[ACT]^C
963 GAGCAC
BspMI 538 ACCTGC ACCTGC(4/8)
879 GCAGGT
BstXI 254 CCAAACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
848 CCATCGTTTTGG
BstYI 364 AGATCC [GA]^GATC[CT]
1120 AGATCC
DdeI 382 CTCAG C^T[ACGT]AG
757 CTTAG
EarI 918 CTCTTC CTCTTC(1/4)
1018 CTCTTC
Ksp632I 918 CTCTTC CTCTTC(1/4)
1018 CTCTTC
MaeI 578 CTAG C^TAG
1042 CTAG
MflI 364 AGATCC [GA]^GATC[CT]
1120 AGATCC
PflMI 657 CCACATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1031 CCAGTTGGTGG
PleI 3 GACTC GAGTC(4/5)
403 GACTC
PstI 1131 CTGCAG CTGCA^G
1143 CTGCAG
RsaI 864 GTAC GT^AC
883 GTAC
SfaNI 482 GATGC GCATC(5/9)
947 GCATC
Tsp45I 769 GTCAC ^GT[GC]AC
1109 GTGAC
TspRI 109 CAGTG CA[GC]TG[ACGT][ACGT]^
1050 CACTG
Van91I 657 CCACATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1031 CCAGTTGGTGG
XcmI 251 CCACCAAACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
285 CCAGGTAGTTGTTGG
XhoII 364 AGATCC [GA]^GATC[CT]
1120 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AluI BbvI BciVI BsaI BsaJI
BsgI BsiYI BslI BsmAI BsrI
BssKI BstF5I BstNI Cfr13I DpnII
EcoRII EcoT14I Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeIII MboI
MboII MvaI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfcI
StyI TfiI TseI Tsp509I