(Circular) (Six-base) MAPSORT of: pFN21AE9514.seq Check: 6475 from: 1 to: 5890 pFN21AE9514 5890 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2552 2552 Size: 5890 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3700 278 Size: 53 83 186 936 2164 2468 Fragments arranged by size: 2468 2164 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3233 1937 Size: 1296 4594 Acc65I G'GTAC_C Cuts at: 3211 3211 Size: 5890 AclI AA'CG_TT Cuts at: 1563 4017 4390 1563 Size: 2454 373 3063 Fragments arranged by size: 3063 2454 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3953 5337 5605 1632 Size: 2321 1384 268 1917 Fragments arranged by size: 2321 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5693 AflIII A'CryG_T Cuts at: 1203 4789 5766 1203 Size: 3586 977 1327 Fragments arranged by size: 3586 1327 977 AgeI A'CCGG_T Cuts at: 2141 4721 2141 Size: 2580 3310 AhdI GACnn_n'nnGTC Cuts at: 4619 4619 Size: 5890 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5890 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5890 AlwNI CAG_nnn'CTG Cuts at: 3135 3144 3633 5205 3135 Size: 9 489 1572 3820 Fragments arranged by size: 3820 1572 489 9 ApaLI G'TGCA_C Cuts at: 3947 5103 3947 Size: 1156 4734 ApoI r'AATT_y Cuts at: 1493 2314 2492 3199 3425 3577 5686 1493 Size: 821 178 707 226 152 2109 1697 Fragments arranged by size: 2109 1697 821 707 226 178 152 AseI AT'TA_AT Cuts at: 160 1032 4444 160 Size: 872 3412 1606 Fragments arranged by size: 3412 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5890 AvaI C'yCGr_G Cuts at: 1870 1943 3215 1870 Size: 73 1272 4545 Fragments arranged by size: 4545 1272 73 AvrII C'CTAG_G Cuts at: 2582 2582 Size: 5890 BamHI G'GATC_C Cuts at: 3220 3587 3220 Size: 367 5523 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2391 3211 4666 618 Size: 325 217 627 604 820 1455 1842 Fragments arranged by size: 1842 1455 820 627 604 325 217 BanII G_rGCy'C Cuts at: 1391 2832 3209 1391 Size: 1441 377 4072 Fragments arranged by size: 4072 1441 377 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2551 928 Size: 971 652 4267 Fragments arranged by size: 4267 971 652 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2836 2870 4081 4115 1903 Size: 34 899 34 1211 34 3678 Fragments arranged by size: 3678 1211 899 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2052 2110 2351 3782 4992 2052 Size: 58 241 1431 1210 2950 Fragments arranged by size: 2950 1431 1210 241 58 BclI T'GATC_A Cuts at: 1548 2568 1548 Size: 1020 4870 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4501 136 Size: 107 122 71 631 605 2829 1525 Fragments arranged by size: 2829 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5885 5885 Size: 5890 BlpI GC'TnA_GC Cuts at: 3307 3307 Size: 5890 Bme1580I G_kGCm'C Cuts at: 1790 3951 5107 1790 Size: 2161 1156 2573 Fragments arranged by size: 2573 2161 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5890 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3621 4579 442 Size: 416 53 83 399 24 2204 958 1753 Fragments arranged by size: 2204 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5890 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2575 4550 1239 Size: 1336 1975 2579 Fragments arranged by size: 2579 1975 1336 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2572 4015 4880 5178 2572 Size: 1443 865 298 3284 Fragments arranged by size: 3284 1443 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2150 2435 2639 4553 882 Size: 617 651 285 204 1914 2219 Fragments arranged by size: 2219 1914 651 617 285 204 BsaAI yAC'GTr Cuts at: 493 1204 3681 493 Size: 711 2477 2702 Fragments arranged by size: 2702 2477 711 BsaBI GATnn'nnATC Cuts at: 2573 5884 2573 Size: 3311 2579 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3697 4079 4714 275 Size: 53 83 186 936 2164 382 635 1451 Fragments arranged by size: 2164 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2141 4323 4721 4995 5142 5553 2141 Size: 2182 398 274 147 411 2478 Fragments arranged by size: 2478 2182 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5665 Fragments arranged by size: 5665 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5890 BseYI C'CCAG_C Cuts at: 2652 3119 5093 5589 2652 Size: 467 1974 496 2953 Fragments arranged by size: 2953 1974 496 467 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2137 2223 3045 1163 Size: 573 401 86 822 4008 Fragments arranged by size: 4008 822 573 401 86 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3340 4101 5129 1540 Size: 275 186 1339 761 1028 2301 Fragments arranged by size: 2301 1339 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2972 3951 4036 5107 2972 Size: 979 85 1071 3755 Fragments arranged by size: 3755 1071 979 85 BsmI GAATG_Cn' Cuts at: 2126 3401 3494 2126 Size: 1275 93 4522 Fragments arranged by size: 4522 1275 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2832 2972 3209 3951 4036 5107 1391 Size: 399 1042 140 237 742 85 1071 2174 Fragments arranged by size: 2174 1071 1042 742 399 237 140 85 BspEI T'CCGG_A Cuts at: 5553 5553 Size: 5890 BspHI T'CATG_A Cuts at: 3172 3779 5509 3172 Size: 607 1730 3553 Fragments arranged by size: 3553 1730 607 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2552 2875 3245 844 Size: 1708 323 370 3489 Fragments arranged by size: 3489 1708 370 323 BsrBI CCG'CTC Cuts at: 1394 1823 3777 1394 Size: 429 1954 3507 Fragments arranged by size: 3507 1954 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3069 4385 4559 65 Size: 1183 1821 1316 174 1396 Fragments arranged by size: 1821 1396 1316 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2141 4534 4721 1700 Size: 110 331 2393 187 2869 Fragments arranged by size: 2869 2393 331 187 110 BsrGI T'GTAC_A Cuts at: 96 2868 96 Size: 2772 3118 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5890 BssSI C'ACGA_G Cuts at: 3950 4962 3950 Size: 1012 4878 BstAPI GCAn_nnn'nTGC Cuts at: 794 3144 794 Size: 2350 3540 BstBI TT'CG_AA Cuts at: 4694 4694 Size: 5890 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5890 BstXI CCAn_nnnn'nTGG Cuts at: 2266 2860 2266 Size: 594 5296 BstYI r'GATC_y Cuts at: 1972 2369 3125 3220 3587 3972 3989 5441 5527 Size: 397 756 95 367 385 17 1452 86 Cuts at: 5527 5539 5885 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 756 397 385 367 346 95 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2445 513 Size: 552 1380 3958 Fragments arranged by size: 3958 1380 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5151 Fragments arranged by size: 5151 683 56 BtsI GCAGTG_nn' Cuts at: 3054 3406 4200 4220 3054 Size: 352 794 20 4724 Fragments arranged by size: 4724 794 352 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5890 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5855 DraI TTT'AAA Cuts at: 3194 3194 Size: 5890 DraIII CAC_nnn'GTG Cuts at: 2765 2765 Size: 5890 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2182 4897 809 Size: 507 866 2715 1802 Fragments arranged by size: 2715 1802 866 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3337 4226 5855 Size: 54 1425 50 275 18 1507 889 1629 Cuts at: 5855 8 Size: 43 Fragments arranged by size: 1629 1507 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3337 1537 Size: 275 1525 4090 Fragments arranged by size: 4090 1525 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2929 3029 3820 1353 Size: 1576 100 791 3423 Fragments arranged by size: 3423 1576 791 100 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4477 4851 4997 387 Size: 1064 138 2888 374 146 1280 Fragments arranged by size: 2888 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2575 3953 4550 5337 5605 1239 Size: 393 943 1378 597 787 268 1524 Fragments arranged by size: 1524 1378 943 787 597 393 268 EcoRI G'AATT_C Cuts at: 3199 3577 3199 Size: 378 5512 FspI TGC'GCA Cuts at: 4396 4396 Size: 5890 HaeII r_GCGC'y Cuts at: 1678 5037 1678 Size: 3359 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 1999 2031 3117 3149 4531 Size: 32 135 32 427 32 1086 32 1382 Cuts at: 4531 4563 4605 4637 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1382 1086 427 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3234 3480 669 Size: 988 281 1296 246 3079 Fragments arranged by size: 3079 1296 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5890 HpaI GTT'AAC Cuts at: 3480 3480 Size: 5890 KpnI G_GTAC'C Cuts at: 3215 3215 Size: 5890 MfeI C'AATT_G Cuts at: 3489 3489 Size: 5890 MluI A'CGCG_T Cuts at: 5766 5766 Size: 5890 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2281 3107 5004 5188 1305 Size: 976 826 1897 184 2007 Fragments arranged by size: 2007 1897 976 826 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4068 Fragments arranged by size: 4068 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3848 4207 4366 518 Size: 648 2682 359 159 2042 Fragments arranged by size: 2682 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2182 3306 3983 5131 5376 1606 Size: 576 1124 677 1148 245 2120 Fragments arranged by size: 2120 1148 1124 677 576 245 NcoI C'CATG_G Cuts at: 513 1065 2445 513 Size: 552 1380 3958 Fragments arranged by size: 3958 1380 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5890 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5890 NotI GC'GGCC_GC Cuts at: 3337 3337 Size: 5890 NspI r_CATG'y Cuts at: 1132 1229 2668 3248 4793 1132 Size: 97 1439 580 1545 2229 Fragments arranged by size: 2229 1545 1439 580 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5890 PciI A'CATG_T Cuts at: 4789 4789 Size: 5890 PflMI CCAn_nnn'nTGG Cuts at: 1108 2668 3042 1108 Size: 1560 374 3956 Fragments arranged by size: 3956 1560 374 PmeI GTTT'AAAC Cuts at: 3194 3194 Size: 5890 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3959 3991 5472 5504 3959 Size: 32 1481 32 4345 Fragments arranged by size: 4345 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5890 PsiI TTA'TAA Cuts at: 3460 3460 Size: 5890 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2897 2929 2897 Size: 32 5858 PstI C_TGCA'G Cuts at: 830 3140 3152 3242 830 Size: 2310 12 90 3478 Fragments arranged by size: 3478 2310 90 12 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5890 PvuII CAG'CTG Cuts at: 2182 2182 Size: 5890 SalI G'TCGA_C Cuts at: 1936 3232 1936 Size: 1296 4594 SbfI CC_TGCA'GG Cuts at: 3242 3242 Size: 5890 ScaI AGT'ACT Cuts at: 4138 5850 4138 Size: 1712 4178 SfcI C'TryA_G Cuts at: 826 1045 2400 2899 3136 3148 3238 4373 5054 Size: 219 1355 499 237 12 90 1135 681 Cuts at: 5054 5245 826 Size: 191 1471 Fragments arranged by size: 1471 1355 1135 681 499 237 219 191 90 12 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5890 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5890 SmaI CCC'GGG Cuts at: 1872 3217 1872 Size: 1345 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2551 3994 4895 5157 820 Size: 197 926 608 1443 901 262 1553 Fragments arranged by size: 1553 1443 926 901 608 262 197 SnaBI TAC'GTA Cuts at: 493 3681 493 Size: 3188 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5890 SphI G_CATG'C Cuts at: 1132 3248 1132 Size: 2116 3774 SspI AAT'ATT Cuts at: 5 52 3814 5 Size: 47 3762 2081 Fragments arranged by size: 3762 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5890 StyI C'CwwG_G Cuts at: 513 1065 2412 2445 2582 3329 513 Size: 552 1347 33 137 747 3074 Fragments arranged by size: 3074 1347 747 552 137 33 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3917 3934 4087 4272 4717 325 Size: 1255 2337 17 153 185 445 1498 Fragments arranged by size: 2337 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2868 4136 5848 Size: 274 80 33 51 1443 891 1268 1712 Cuts at: 5848 96 Size: 138 Fragments arranged by size: 1712 1443 1268 891 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5850 5882 5850 Size: 32 5858 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5890 XbaI T'CTAG_A Cuts at: 3226 3226 Size: 5890 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2263 2297 2263 Size: 34 5856 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5890 XmaI C'CCGG_G Cuts at: 1870 3215 1870 Size: 1345 4545 XmnI GAAnn'nnTTC Cuts at: 4019 4019 Size: 5890 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3698 276 Size: 53 83 186 936 2164 2468 Fragments arranged by size: 2468 2164 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI