################################## # NO CUT ENZYMES ( 140 enzymes) # ################################## AatII AccIII AclI AcsI AflII AflIII AgeI AhdI ApaI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BalI BamHI BbrPI BbsI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaI BsaWI BseAI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII Bst1107I BstBI BstXI BstYI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DrdI Eam1105I EarI EcoNI EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI Ksp632I KspI MamI MboII MfeI MflI MluI MluNI MroI MscI MseI MslI MspA1I MunI NaeI NcoI NdeI NgoMIV NheI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TaqI TfiI Tru9I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### Acc65I 518 GGTACC G^GTACC AccI 745 GTCTAC GT^[AC][GT]AC AccII 325 CGCG CG^CG Alw44I 138 GTGCAC G^TGCAC Aor51HI 716 AGCGCT AGC^GCT ApaLI 138 GTGCAC G^TGCAC Asp718I 518 GGTACC G^GTACC AvaI 463 CCCGGG C^[CT]CG[GA]G BbvCI 35 CCTCAGC CCTCAGC(-5/-2) BfaI 587 CTAG C^TAG BglI 169 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 688 CTCCAG CTGGAG(16/14) BsaAI 135 TACGTG [CT]AC^GT[GA] BseRI 294 GAGGAG GAGGAG(10/8) BsiEI 252 CGGCCG CG[GA][CT]^CG BsoBI 463 CCCGGG C^[CT]CG[GA]G BspMI 315 GCAGGT ACCTGC(4/8) BsrDI 534 GCAATG GCAATG(2/0) BsrI 225 CCAGT ACTGG(1/-1) BssSI 697 CTCGTG CACGAG(-5/-1) BstAPI 315 GCAGGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 441 GGTCACC G^GT[ACGT]ACC BstPI 441 GGTCACC G^GT[ACGT]ACC BstUI 325 CGCG CG^CG DsaI 157 CCGTGG C^C[GA][CT]GG EaeI 252 CGGCCG [CT]^GGCC[GA] EagI 252 CGGCCG C^GGCCG EclXI 252 CGGCCG C^GGCCG Eco47III 716 AGCGCT AGC^GCT Eco52I 252 CGGCCG C^GGCCG EcoO65I 441 GGTCACC G^GT[ACGT]ACC HgaI 342 GACGC GACGC(5/10) KpnI 518 GGTACC GGTAC^C MaeI 587 CTAG C^TAG MaeII 136 ACGT A^CGT MvnI 325 CGCG CG^CG NotI 251 GCGGCCGC GC^GGCCGC PshAI 640 GACTCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 391 CTGCAG CTGCA^G SacI 147 GAGCTC GAGCT^C SfaNI 789 GATGC GCATC(5/9) SmaI 463 CCCGGG CCC^GGG Tsp509I 123 AATT ^AATT XmaCI 463 CCCGGG C^CCGGG XmaI 463 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AcyI 188 GGCGCC G[GA]^CG[CT]C 208 GGCGCC AfaI 519 GTAC GT^AC 765 GTAC AlwI 261 GATCC GGATC(4/5) 413 GGATC AlwNI 316 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG 768 CAGAAGCTG AocI 6 CCTGAGG CC^T[ACGT]AGG 291 CCTGAGG AspHI 138 GTGCAC G[AT]GC[AT]^C 147 GAGCTC BanII 18 GGGCTC G[GA]GC[CT]^C 147 GAGCTC BbeI 188 GGCGCC GGCGC^C 208 GGCGCC BsaHI 188 GGCGCC G[GA]^CG[CT]C 208 GGCGCC BsgI 614 GTGCAG GTGCAG(16/14) 675 GTGCAG BsiHKAI 138 GTGCAC G[AT]GC[AT]^C 147 GAGCTC BstF5I 108 GGATG GGATG(2/0) 561 GGATG Bsu36I 6 CCTGAGG CC^T[ACGT]AGG 291 CCTGAGG DpnII 261 GATC ^GATC 414 GATC Eco81I 6 CCTGAGG CC^T[ACGT]AGG 291 CCTGAGG FokI 108 GGATG GGATG(9/13) 561 GGATG Hin1I 188 GGCGCC G[GA]^CG[CT]C 208 GGCGCC HinfI 640 GACTC G^A[ACGT]TC 655 GACTC KasI 188 GGCGCC G^GCGCC 208 GGCGCC MboI 261 GATC ^GATC 414 GATC NarI 188 GGCGCC GG^CGCC 208 GGCGCC NdeII 261 GATC ^GATC 414 GATC PleI 640 GACTC GAGTC(4/5) 655 GACTC RsaI 519 GTAC GT^AC 765 GTAC Sau3AI 261 GATC ^GATC 414 GATC SfoI 188 GGCGCC GGC^GCC 208 GGCGCC StuI 449 AGGCCT AGG^CCT 578 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AciI AluI AvaII BanI BbvI BcnI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BssKI BstNI Cac8I CfoI Cfr13I DdeI DraII EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeIII MnlI MspI MvaI MwoI NciI NlaIII NlaIV PpuMI Sau96I ScrFI SfcI TseI Tsp45I TspRI