(Circular) (Six-base) MAPSORT of: pFN21AE3096.seq Check: 5964 from: 1 to: 5506 pFN21AE3096 5506 bp With 182 enzymes: SgfI * March 28, 2012 12:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2311 2311 Size: 5506 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3316 278 Size: 53 83 186 936 1780 2468 Fragments arranged by size: 2468 1780 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2751 2849 1937 Size: 814 98 4594 Fragments arranged by size: 4594 814 98 Acc65I G'GTAC_C Cuts at: 2523 2827 2523 Size: 304 5202 AclI AA'CG_TT Cuts at: 1563 3633 4006 1563 Size: 2070 373 3063 Fragments arranged by size: 3063 2070 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2255 3569 4953 5221 1632 Size: 623 1314 1384 268 1917 Fragments arranged by size: 1917 1384 1314 623 268 AfeI AGC'GCT Cuts at: 2723 2723 Size: 5506 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5309 AflIII A'CryG_T Cuts at: 1203 4405 5382 1203 Size: 3202 977 1327 Fragments arranged by size: 3202 1327 977 AgeI A'CCGG_T Cuts at: 4337 4337 Size: 5506 AhdI GACnn_n'nnGTC Cuts at: 4235 4235 Size: 5506 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5506 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5506 AlwNI CAG_nnn'CTG Cuts at: 2326 2778 3249 4821 2326 Size: 452 471 1572 3011 Fragments arranged by size: 3011 1572 471 452 ApaLI G'TGCA_C Cuts at: 2143 3563 4719 2143 Size: 1420 1156 2930 Fragments arranged by size: 2930 1420 1156 ApoI r'AATT_y Cuts at: 1493 2815 3041 3193 5302 1493 Size: 1322 226 152 2109 1697 Fragments arranged by size: 2109 1697 1322 226 152 AseI AT'TA_AT Cuts at: 160 1032 4060 160 Size: 872 3028 1606 Fragments arranged by size: 3028 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5506 AvaI C'yCGr_G Cuts at: 1870 1943 2468 2831 1870 Size: 73 525 363 4545 Fragments arranged by size: 4545 525 363 73 BamHI G'GATC_C Cuts at: 2836 3203 2836 Size: 367 5139 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2193 2213 2523 2827 4282 Size: 325 217 627 406 20 310 304 1455 Cuts at: 4282 618 Size: 1842 Fragments arranged by size: 1842 1455 627 406 325 310 304 217 20 BanII G_rGCy'C Cuts at: 1391 2027 2156 2825 1391 Size: 636 129 669 4072 Fragments arranged by size: 4072 669 636 129 BbeI G_GCGC'C Cuts at: 2197 2217 2197 Size: 20 5486 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4535 BbvCI CC'TCA_GC Cuts at: 2041 2041 Size: 5506 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3697 3731 1903 Size: 34 1760 34 3678 Fragments arranged by size: 3678 1760 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3398 4608 3398 Size: 1210 4296 BclI T'GATC_A Cuts at: 1548 1548 Size: 5506 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2180 4117 136 Size: 107 122 71 631 605 508 1937 1525 Fragments arranged by size: 1937 1525 631 605 508 122 107 71 BglII A'GATC_T Cuts at: 5501 5501 Size: 5506 BlpI GC'TnA_GC Cuts at: 2923 2923 Size: 5506 Bme1580I G_kGCm'C Cuts at: 1790 2147 3567 4723 1790 Size: 357 1420 1156 2573 Fragments arranged by size: 2573 1420 1156 357 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5506 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3237 4195 442 Size: 416 53 83 399 24 1820 958 1753 Fragments arranged by size: 1820 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5506 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2708 2708 Size: 5506 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2678 4166 1239 Size: 1439 1488 2579 Fragments arranged by size: 2579 1488 1439 Bpu10I CC'TnA_GC Cuts at: 2041 2041 Size: 5506 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3631 4496 4794 3631 Size: 865 298 4343 Fragments arranged by size: 4343 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4169 882 Size: 617 2670 2219 Fragments arranged by size: 2670 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2142 3297 493 Size: 711 938 1155 2702 Fragments arranged by size: 2702 1155 938 711 BsaBI GATnn'nnATC Cuts at: 5500 5500 Size: 5506 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2194 2214 3313 3695 Size: 53 83 186 936 661 20 1099 382 Cuts at: 3695 4330 275 Size: 635 1451 Fragments arranged by size: 1451 1099 936 661 635 382 186 83 53 20 BsaWI w'CCGG_w Cuts at: 3939 4337 4611 4758 5169 3939 Size: 398 274 147 411 4276 Fragments arranged by size: 4276 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2348 2378 1178 Size: 30 165 30 945 30 4306 Fragments arranged by size: 4306 945 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2314 1186 Size: 1128 4378 BseYI C'CCAG_C Cuts at: 2157 2316 2493 4709 5205 2157 Size: 159 177 2216 496 2458 Fragments arranged by size: 2458 2216 496 177 159 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2640 2701 1163 Size: 573 904 61 3968 Fragments arranged by size: 3968 904 573 61 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2260 2956 3717 4745 1540 Size: 275 186 259 696 761 1028 2301 Fragments arranged by size: 2301 1028 761 696 275 259 186 BsiHKAI G_wGCw'C Cuts at: 2147 2156 3567 3652 4723 2147 Size: 9 1411 85 1071 2930 Fragments arranged by size: 2930 1411 1071 85 9 BsmI GAATG_Cn' Cuts at: 3017 3110 3017 Size: 93 5413 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2027 2147 2156 2825 3567 3652 4723 Size: 399 237 120 9 669 742 85 1071 Cuts at: 4723 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 669 399 237 120 85 9 BspEI T'CCGG_A Cuts at: 5169 5169 Size: 5506 BspHI T'CATG_A Cuts at: 3395 5125 3395 Size: 1730 3776 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2311 2861 844 Size: 1467 550 3489 Fragments arranged by size: 3489 1467 550 BsrBI CCG'CTC Cuts at: 1394 1823 3393 1394 Size: 429 1570 3507 Fragments arranged by size: 3507 1570 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2546 4001 4175 65 Size: 1183 1298 1455 174 1396 Fragments arranged by size: 1455 1396 1298 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4150 4337 1700 Size: 110 2340 187 2869 Fragments arranged by size: 2869 2340 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5506 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5506 BssSI C'ACGA_G Cuts at: 2702 3566 4578 2702 Size: 864 1012 3630 Fragments arranged by size: 3630 1012 864 BstAPI GCAn_nnn'nTGC Cuts at: 794 2326 794 Size: 1532 3974 BstBI TT'CG_AA Cuts at: 4310 4310 Size: 5506 BstEII G'GTnAC_C Cuts at: 1184 2446 1184 Size: 1262 4244 BstYI r'GATC_y Cuts at: 1972 2836 3203 3588 3605 5057 5143 5155 5501 Size: 864 367 385 17 1452 86 12 346 Cuts at: 5501 1972 Size: 1977 Fragments arranged by size: 1977 1452 864 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2012 2297 2012 Size: 285 5221 BtgI C'CryG_G Cuts at: 513 1065 2162 513 Size: 552 1097 3857 Fragments arranged by size: 3857 1097 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2500 487 Size: 683 56 1274 3493 Fragments arranged by size: 3493 1274 683 56 BtsI GCAGTG_nn' Cuts at: 2241 2251 3022 3816 3836 2241 Size: 10 771 794 20 3911 Fragments arranged by size: 3911 794 771 20 10 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5506 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5471 DraI TTT'AAA Cuts at: 2810 2810 Size: 5506 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4513 809 Size: 507 3197 1802 Fragments arranged by size: 3197 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2257 2953 3842 Size: 54 1425 50 275 18 427 696 889 Cuts at: 3842 5471 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 696 427 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2257 2953 1537 Size: 275 445 696 4090 Fragments arranged by size: 4090 696 445 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3436 1353 Size: 2083 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4093 4467 4613 387 Size: 1064 138 2504 374 146 1280 Fragments arranged by size: 2504 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2154 2154 Size: 5506 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2255 2678 3569 4166 4953 5221 1239 Size: 393 623 423 891 597 787 268 1524 Fragments arranged by size: 1524 891 787 623 597 423 393 268 EcoO109I rG'GnC_Cy Cuts at: 2031 2186 2472 2669 2698 2031 Size: 155 286 197 29 4839 Fragments arranged by size: 4839 286 197 155 29 EcoRI G'AATT_C Cuts at: 2815 3193 2815 Size: 378 5128 FspI TGC'GCA Cuts at: 4012 4012 Size: 5506 HaeII r_GCGC'y Cuts at: 1678 2197 2217 2725 4653 1678 Size: 519 20 508 1928 2531 Fragments arranged by size: 2531 1928 519 508 20 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4147 4179 4221 4253 1373 Size: 32 135 32 2575 32 42 32 2626 Fragments arranged by size: 2626 2575 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2850 3096 669 Size: 988 281 912 246 3079 Fragments arranged by size: 3079 988 912 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5506 HpaI GTT'AAC Cuts at: 3096 3096 Size: 5506 KasI G'GCGC_C Cuts at: 2193 2213 2193 Size: 20 5486 KpnI G_GTAC'C Cuts at: 2527 2831 2527 Size: 304 5202 MfeI C'AATT_G Cuts at: 3105 3105 Size: 5506 MluI A'CGCG_T Cuts at: 5382 5382 Size: 5506 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4620 4804 1305 Size: 3315 184 2007 Fragments arranged by size: 3315 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3684 Fragments arranged by size: 3684 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3464 3823 3982 518 Size: 648 2298 359 159 2042 Fragments arranged by size: 2298 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2922 3599 4747 4992 1606 Size: 1316 677 1148 245 2120 Fragments arranged by size: 2120 1316 1148 677 245 NarI GG'CG_CC Cuts at: 2194 2214 2194 Size: 20 5486 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4954 NdeI CA'TA_TG Cuts at: 387 387 Size: 5506 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5506 NotI GC'GGCC_GC Cuts at: 2257 2953 2257 Size: 696 4810 NspI r_CATG'y Cuts at: 1132 1229 2864 4409 1132 Size: 97 1635 1545 2229 Fragments arranged by size: 2229 1635 1545 97 PasI CC'CwG_GG Cuts at: 1348 2281 2632 1348 Size: 933 351 4222 Fragments arranged by size: 4222 933 351 PciI A'CATG_T Cuts at: 4405 4405 Size: 5506 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5506 PfoI T'CCnGG_A Cuts at: 2366 2366 Size: 5506 PmeI GTTT'AAAC Cuts at: 2810 2810 Size: 5506 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3575 3607 5088 5120 3575 Size: 32 1481 32 3961 Fragments arranged by size: 3961 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2031 2186 2472 2669 2031 Size: 155 286 197 4868 Fragments arranged by size: 4868 286 197 155 PshAI GACnn'nnGTC Cuts at: 1613 2649 1613 Size: 1036 4470 PsiI TTA'TAA Cuts at: 3076 3076 Size: 5506 PstI C_TGCA'G Cuts at: 830 2400 2858 830 Size: 1570 458 3478 Fragments arranged by size: 3478 1570 458 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5506 SacI G_AGCT'C Cuts at: 2156 2156 Size: 5506 SalI G'TCGA_C Cuts at: 1936 2848 1936 Size: 912 4594 SanDI GG'GwC_CC Cuts at: 2186 2669 2186 Size: 483 5023 SbfI CC_TGCA'GG Cuts at: 2858 2858 Size: 5506 ScaI AGT'ACT Cuts at: 3754 5466 3754 Size: 1712 3794 SfcI C'TryA_G Cuts at: 826 1045 2088 2396 2402 2854 3989 4670 4861 Size: 219 1043 308 6 452 1135 681 191 Cuts at: 4861 826 Size: 1471 Fragments arranged by size: 1471 1135 1043 681 452 308 219 191 6 SfoI GGC'GCC Cuts at: 2195 2215 2195 Size: 20 5486 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5506 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5506 SmaI CCC'GGG Cuts at: 1872 2470 2833 1872 Size: 598 363 4545 Fragments arranged by size: 4545 598 363 SmlI C'TyrA_G Cuts at: 820 1017 1943 3610 4511 4773 820 Size: 197 926 1667 901 262 1553 Fragments arranged by size: 1667 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3297 493 Size: 2804 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5506 SphI G_CATG'C Cuts at: 1132 2864 1132 Size: 1732 3774 SspI AAT'ATT Cuts at: 5 52 3430 5 Size: 47 3378 2081 Fragments arranged by size: 3378 2081 47 StuI AGG'CCT Cuts at: 1517 2456 2585 1517 Size: 939 129 4438 Fragments arranged by size: 4438 939 129 StyI C'CwwG_G Cuts at: 513 1065 2945 513 Size: 552 1880 3074 Fragments arranged by size: 3074 1880 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2330 2465 3533 3550 3703 3888 4333 Size: 1255 750 135 1068 17 153 185 445 Cuts at: 4333 325 Size: 1498 Fragments arranged by size: 1498 1255 1068 750 445 185 153 135 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2769 3752 5464 Size: 274 80 33 51 1443 792 983 1712 Cuts at: 5464 96 Size: 138 Fragments arranged by size: 1712 1443 983 792 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2064 2096 2558 2590 2661 2693 5466 5498 2064 Size: 32 462 32 71 32 2773 32 2072 Fragments arranged by size: 2773 2072 462 71 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5506 XbaI T'CTAG_A Cuts at: 2842 2842 Size: 5506 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5506 XmaI C'CCGG_G Cuts at: 1870 2468 2831 1870 Size: 598 363 4545 Fragments arranged by size: 4545 598 363 XmnI GAAnn'nnTTC Cuts at: 3635 3635 Size: 5506 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3314 276 Size: 53 83 186 936 1780 2468 Fragments arranged by size: 2468 1780 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SapI SexAI SfiI SrfI SwaI XcmI