################################## # NO CUT ENZYMES ( 171 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AfaI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII BalI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BseAI BsgI BsiEI BsiHKAI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MseI MspA1I MunI MvnI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### AflIII 599 ACATGT A^C[GA][CT]GT BamHI 770 GGATCC G^GATCC BbsI 66 GTCTTC GAAGAC(2/6) BcnI 396 CCCGG CC^[GC]GG BpmI 382 CTGGAG CTGGAG(16/14) BpuAI 66 GTCTTC GAAGAC(2/6) BsaWI 335 ACCGGA [AT]^CCGG[AT] BseRI 559 GAGGAG GAGGAG(10/8) BsmBI 693 CGTCTC CGTCTC(1/5) BspLU11I 599 ACATGT A^CATGT BsrI 95 CCAGT ACTGG(1/-1) BstYI 770 GGATCC [GA]^GATC[CT] MaeII 761 ACGT A^CGT MboII 67 TCTTC GAAGA(8/7) MflI 770 GGATCC [GA]^GATC[CT] MslI 361 CACGACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 610 GCCGGC GCC^GGC NciI 396 CCCGG CC^[GC]GG NgoMIV 610 GCCGGC G^CCGGC NspI 599 ACATGT [GA]CATG^[CT] PpuMI 209 GGGTCCT [GA]G^G[AT]CC[CT] PstI 388 CTGCAG CTGCA^G SfaNI 689 GCATC GCATC(5/9) SfcI 388 CTGCAG C^T[GA][CT]AG SmlI 753 CTCAAG C^T[CT][GA]AG SpeI 159 ACTAGT A^CTAGT Tsp45I 647 GTGAC ^GT[GC]AC Tsp509I 511 AATT ^AATT XhoII 770 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AccI 574 GTCTAC GT^[AC][GT]AC 739 GTCTAC AlwI 771 GATCC GGATC(4/5) 770 GGATC ApaI 657 GGGCCC GGGCC^C 822 GGGCCC AvrII 255 CCTAGG C^CTAGG 270 CCTAGG BanI 460 GGCACC G^G[CT][GA]CC 487 GGCACC BlnI 255 CCTAGG C^CTAGG 270 CCTAGG BsmFI 91 GGGAC GGGAC(10/14) 393 GTCCC BspMI 121 ACCTGC ACCTGC(4/8) 294 GCAGGT BsrFI 610 GCCGGC [GA]^CCGG[CT] 728 ACCGGC BstF5I 417 CATCC GGATG(2/0) 540 CATCC Cfr10I 610 GCCGGC [GA]^CCGG[CT] 728 ACCGGC DdeI 137 CTCAG C^T[ACGT]AG 552 CTCAG FokI 417 CATCC GGATG(9/13) 540 CATCC MaeIII 647 GTGAC ^GT[ACGT]AC 671 GTAAC ######################################### # 3 OR MORE CUT ENZYMES ( 44 enzymes) # ######################################### AciI AluI AvaII BanII BbvI BfaI BmyI BsaJI BsiYI BslI BsmAI Bsp1286I BssKI BstNI Cac8I Cfr13I DpnII DraII EcoO109I EcoRII EcoT14I Fnu4HI HaeIII HapII HinfI HpaII HphI ItaI MaeI MboI MnlI MspI MvaI MwoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI StyI TseI TspRI